Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ethanolamine kinase 2

Gene

Etnk2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Highly specific for ethanolamine phosphorylation. Does not have choline kinase activity.1 Publication

Caution

According to PubMed:16861741, disruption of the gene causes increased prevalence of placental thrombosis, reduced litter size and increased pup mortality. No such effect was observed by PubMed:18755794. One possible explanation lies in the fact that PubMed:16861741 observed a remarkable 100% survival of control pups and 16% mortality for mutant pups, while PubMed:18755794 observed 16% mortality for both wild-type and mutant pups. According to PubMed:18755794, the construct used in PubMed:16861741 may lead to expression of a truncated transcript that might have deleterious effects.Curated

Catalytic activityi

ATP + ethanolamine = ADP + O-phosphoethanolamine.1 Publication

Pathwayi: phosphatidylethanolamine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes phosphatidylethanolamine from ethanolamine.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Choline/ethanolamine kinase (Chkb), Choline kinase alpha (Chka), Ethanolamine kinase 1 (Etnk1), Ethanolamine kinase 2 (Etnk2)
  2. Ethanolamine-phosphate cytidylyltransferase (Pcyt2)
  3. Choline/ethanolaminephosphotransferase 1 (Cept1), Ethanolaminephosphotransferase 1 (Selenoi)
This subpathway is part of the pathway phosphatidylethanolamine biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes phosphatidylethanolamine from ethanolamine, the pathway phosphatidylethanolamine biosynthesis and in Phospholipid metabolism.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ethanolamine kinase activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processLipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.1.82 3474
ReactomeiR-MMU-1483213 Synthesis of PE
UniPathwayi
UPA00558;UER00741

Names & Taxonomyi

Protein namesi
Recommended name:
Ethanolamine kinase 2 (EC:2.7.1.82)
Short name:
EKI 2
Alternative name(s):
Ethanolamine kinase-like protein
Gene namesi
Name:Etnk2
Synonyms:Tuc1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:2443760 Etnk2

Pathology & Biotechi

Disruption phenotypei

No visible phenotype in adults. No significant effect is seen on liver phospholipid metabolism, neural development, or testicular function. No abnormalities are detected in embryonic and adult testis morphology, differentiation, function, or fertility. PubMed:16861741 shows maternal-specific failure to support late embryonic development, resulting in reduced perinatal size and survival and suggesting compromised placental function.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003939621 – 385Ethanolamine kinase 2Add BLAST385

Proteomic databases

MaxQBiA7MCT6
PaxDbiA7MCT6
PRIDEiA7MCT6

PTM databases

iPTMnetiA7MCT6
PhosphoSitePlusiA7MCT6

Expressioni

Tissue specificityi

Expressed in testis and liver. Low expression in ovary and kidney.3 Publications

Developmental stagei

The expression is restricted to the gonads during the sex determination period and throughout embryogenesis. In developing testis, the expression is found only in the Sertoli cells. The expression is strongest at day 11.5, more intense in the testis than the ovary. From 12.5 dpc the expression in the ovary is reduced and disappears. The expression at day 13.5 dpc is restricted to the testis cords.1 Publication

Gene expression databases

BgeeiENSMUSG00000070644 Expressed in 67 organ(s), highest expression level in liver
ExpressionAtlasiA7MCT6 baseline and differential
GenevisibleiA7MCT6 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000114272

Structurei

3D structure databases

ProteinModelPortaliA7MCT6
SMRiA7MCT6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the choline/ethanolamine kinase family.Curated

Phylogenomic databases

eggNOGiKOG4720 Eukaryota
COG0510 LUCA
GeneTreeiENSGT00530000062991
HOGENOMiHOG000004856
HOVERGENiHBG018981
InParanoidiA7MCT6
KOiK00894
OMAiIQELRPH
OrthoDBiEOG091G0FYJ
PhylomeDBiA7MCT6
TreeFamiTF313549

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
SUPFAMiSSF56112 SSF56112, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.iShow all

Isoform 1 (identifier: A7MCT6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAVPPSAPVP CSPFYLRRQE PCPQCSWSME EKAVASAGCW EPPGPPRAAV
60 70 80 90 100
PCFSVTVEQD DILPGALRLI RELRPHWKPE QVRTKRFKDG ITNKLLACYV
110 120 130 140 150
EEDMRDCVLV RVYGERTELL VDRENEVRNF QLLRAHGCAP KLYCTFQNGL
160 170 180 190 200
CYEYVQGVAL GPEHIREPQL FRLIALEMAK IHTIHANGSL PKPTLWHKMH
210 220 230 240 250
RYFTLVKDEI SPSLSADVPK VEVLEQELAW LKEHLSQLDS PVVFCHNDLL
260 270 280 290 300
CKNIIYDSDK GRVCFIDYEY AGYNYQAFDI GNHFNEFAGV NVVDYSRYPA
310 320 330 340 350
RETQVQWLRY YLEAQKGTAA SPREVERLYA QVNKFALASH FFWALWALIQ
360 370 380
NQYSTISFDF LRYAVIRFNQ YFKVKPQVSA LEMPK
Length:385
Mass (Da):44,567
Last modified:October 2, 2007 - v1
Checksum:i46C062858F7FFD29
GO
Isoform 2 (identifier: A7MCT6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     290-385: VNVVDYSRYP...PQVSALEMPK → RGGLL

Show »
Length:294
Mass (Da):33,620
Checksum:i3076342DA02B10CF
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H9KV13H9KV13_MOUSE
Ethanolamine kinase 2
Etnk2
282Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti166R → Q in AAP47267 (PubMed:15161093).Curated1
Sequence conflicti292V → E in AAP47267 (PubMed:15161093).Curated1
Sequence conflicti336A → S in AAP47267 (PubMed:15161093).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_039099290 – 385VNVVD…LEMPK → RGGLL in isoform 2. 1 PublicationAdd BLAST96

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC068906 Genomic DNA No translation available.
BC145351 mRNA Translation: AAI45352.1
BC152310 mRNA Translation: AAI52311.1
AY212244 mRNA Translation: AAP47267.1
CCDSiCCDS15296.2 [A7MCT6-1]
RefSeqiNP_780652.2, NM_175443.5 [A7MCT6-1]
UniGeneiMm.52111

Genome annotation databases

EnsembliENSMUST00000135222; ENSMUSP00000114272; ENSMUSG00000070644 [A7MCT6-1]
GeneIDi214253
KEGGimmu:214253
UCSCiuc007cqg.2 mouse [A7MCT6-1]
uc011wsa.2 mouse [A7MCT6-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiEKI2_MOUSE
AccessioniPrimary (citable) accession number: A7MCT6
Secondary accession number(s): B7ZNN6, D3Z771, Q6XRG2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 18, 2010
Last sequence update: October 2, 2007
Last modified: September 12, 2018
This is version 75 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again