UniProtKB - A7MBE0 (S22A1_BOVIN)
Solute carrier family 22 member 1
SLC22A1
Functioni
Translocates a broad array of organic cations with various structures and molecular weights including the model compounds 1-methyl-4-phenylpyridinium (MPP), tetraethylammonium (TEA), N-1-methylnicotinamide (NMN), 4-(4-(dimethylamino)styryl)-N-methylpyridinium (ASP), the endogenous compounds choline, guanidine, histamine, epinephrine, adrenaline, noradrenaline and dopamine, and the drugs quinine, and metformin. The transport of organic cations is inhibited by a broad array of compounds like tetramethylammonium (TMA), cocaine, lidocaine, NMDA receptor antagonists, atropine, prazosin, cimetidine, TEA and NMN, guanidine, cimetidine, choline, procainamide, quinine, tetrabutylammonium, and tetrapentylammonium. Translocates organic cations in an electrogenic and pH-independent manner. Translocates organic cations across the plasma membrane in both directions. Transports the polyamines spermine and spermidine. Transports pramipexole across the basolateral membrane of the proximal tubular epithelial cells. The choline transport is activated by MMTS. Regulated by various intracellular signaling pathways including inhibition by protein kinase A activation, and endogenously activation by the calmodulin complex, the calmodulin-dependent kinase II and LCK tyrosine kinase (By similarity).
By similaritySites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 445 | Involved in affinity and selectivity of cations as well as in translocationBy similarity | 1 |
GO - Molecular functioni
- monoamine transmembrane transporter activity Source: Ensembl
- neurotransmitter transmembrane transporter activity Source: Ensembl
- organic cation transmembrane transporter activity Source: GO_Central
- pyrimidine nucleoside transmembrane transporter activity Source: Ensembl
- quaternary ammonium group transmembrane transporter activity Source: Ensembl
- toxin transmembrane transporter activity Source: Ensembl
- xenobiotic transmembrane transporter activity Source: Ensembl
GO - Biological processi
- dopamine uptake Source: Ensembl
- norepinephrine transport Source: Ensembl
- organic cation transport Source: GO_Central
- serotonin uptake Source: Ensembl
Keywordsi
Biological process | Ion transport, Transport |
Enzyme and pathway databases
Reactomei | R-BTA-112311, Neurotransmitter clearance R-BTA-181430, Norepinephrine Neurotransmitter Release Cycle R-BTA-2161517, Abacavir transmembrane transport R-BTA-442660, Na+/Cl- dependent neurotransmitter transporters R-BTA-549127, Organic cation transport |
Names & Taxonomyi
Protein namesi | Recommended name: Solute carrier family 22 member 1Alternative name(s): Organic cation transporter 1 |
Gene namesi | Name:SLC22A1 Synonyms:OCT1 |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Organism-specific databases
VEuPathDBi | HostDB:ENSBTAG00000008540 |
VGNCi | VGNC:34719, SLC22A1 |
Subcellular locationi
Plasma membrane
Plasma Membrane
- apical plasma membrane Source: Ensembl
- basolateral plasma membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
- presynapse Source: GOC
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 21 | CytoplasmicSequence analysisAdd BLAST | 21 | |
Transmembranei | 22 – 42 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 43 – 144 | ExtracellularSequence analysisAdd BLAST | 102 | |
Transmembranei | 145 – 165 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 166 – 171 | CytoplasmicSequence analysis | 6 | |
Transmembranei | 172 – 192 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 193 – 196 | ExtracellularSequence analysis | 4 | |
Transmembranei | 197 – 219 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 220 – 232 | CytoplasmicSequence analysisAdd BLAST | 13 | |
Transmembranei | 233 – 253 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 254 – 257 | ExtracellularSequence analysis | 4 | |
Transmembranei | 258 – 278 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 279 – 342 | CytoplasmicSequence analysisAdd BLAST | 64 | |
Transmembranei | 343 – 363 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 364 – 371 | ExtracellularSequence analysis | 8 | |
Transmembranei | 372 – 392 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 393 – 398 | CytoplasmicSequence analysis | 6 | |
Transmembranei | 399 – 418 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 419 – 423 | ExtracellularSequence analysis | 5 | |
Transmembranei | 424 – 446 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 447 – 459 | CytoplasmicSequence analysisAdd BLAST | 13 | |
Transmembranei | 460 – 480 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 481 – 487 | ExtracellularSequence analysis | 7 | |
Transmembranei | 488 – 508 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 509 – 563 | CytoplasmicSequence analysisAdd BLAST | 55 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000333874 | 1 – 563 | Solute carrier family 22 member 1Add BLAST | 563 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 71 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 328 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Glycoprotein, PhosphoproteinProteomic databases
PaxDbi | A7MBE0 |
PRIDEi | A7MBE0 |
Expressioni
Gene expression databases
Bgeei | ENSBTAG00000008540, Expressed in cortex of kidney and 22 other tissues |
Interactioni
Protein-protein interaction databases
STRINGi | 9913.ENSBTAP00000004059 |
Chemistry databases
BindingDBi | A7MBE0 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0255, Eukaryota |
GeneTreei | ENSGT00940000162065 |
HOGENOMi | CLU_001265_33_5_1 |
InParanoidi | A7MBE0 |
OMAi | YLWFSCA |
OrthoDBi | 704438at2759 |
TreeFami | TF315847 |
Family and domain databases
Gene3Di | 1.20.1250.20, 1 hit |
InterProi | View protein in InterPro IPR020846, MFS_dom IPR005828, MFS_sugar_transport-like IPR036259, MFS_trans_sf IPR004749, Orgcat_transp/SVOP IPR005829, Sugar_transporter_CS |
Pfami | View protein in Pfam PF00083, Sugar_tr, 1 hit |
SUPFAMi | SSF103473, SSF103473, 1 hit |
TIGRFAMsi | TIGR00898, 2A0119, 1 hit |
PROSITEi | View protein in PROSITE PS50850, MFS, 1 hit PS00216, SUGAR_TRANSPORT_1, 2 hits |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MLTVDDVLEQ VGEFGWFQKQ TFLILCLLSA AFAPIYVGIV FLAFTPDHRC
60 70 80 90 100
RSPGVAELSR RCGWSLAEEL NYTVPGPGPE SQCLRYEVDW NQSTLGCLDP
110 120 130 140 150
LASLATNGSP LPLGPCEQGW VYDTPGSSIV TEFNLVCDDS WKVDLFQSCV
160 170 180 190 200
NLGFFLGSLG VGYIADRFGR KVCLLATTLT CASLGVLTAV APDYTSLLIF
210 220 230 240 250
RLLQGLVSKG SWTAGYTLIT EFVGLGYRRT VAILYQMAFT VGLVLLSGLA
260 270 280 290 300
YILPHWRWLQ LAVSLPIFLL LFRFWFVPES PRWLLSQKRN TEAIKIMDHI
310 320 330 340 350
AQKNGKLPPA DLKMLSLEED VTEKLSPSFI DLFRTPNLRK YTFILMYLWF
360 370 380 390 400
TSSVVYQGLI MHVGATGGNL YLDFLYSALV EFPAGFIILV TIDRFGRRYP
410 420 430 440 450
LATSNLAAGL ACFLMIFIPH DLPWLNIMVA CVGRMGITIV FQMVCLVNAE
460 470 480 490 500
LFPTFIRNLG MMVCSSLCDL GGVLTPFLVF RLMEVWQGSP LILFAALGLV
510 520 530 540 550
AGGMTLLLPE TKGVTLPETI EDAENLQRKA KPKENKIYLQ VQTSELNTQA
560
AERDASQGTA QQK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC151505 mRNA Translation: AAI51506.1 |
RefSeqi | NP_001094568.1, NM_001101098.2 |
Genome annotation databases
Ensembli | ENSBTAT00000004059; ENSBTAP00000004059; ENSBTAG00000008540 |
GeneIDi | 519461 |
KEGGi | bta:519461 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC151505 mRNA Translation: AAI51506.1 |
RefSeqi | NP_001094568.1, NM_001101098.2 |
3D structure databases
AlphaFoldDBi | A7MBE0 |
SMRi | A7MBE0 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9913.ENSBTAP00000004059 |
Chemistry databases
BindingDBi | A7MBE0 |
Proteomic databases
PaxDbi | A7MBE0 |
PRIDEi | A7MBE0 |
Genome annotation databases
Ensembli | ENSBTAT00000004059; ENSBTAP00000004059; ENSBTAG00000008540 |
GeneIDi | 519461 |
KEGGi | bta:519461 |
Organism-specific databases
CTDi | 6580 |
VEuPathDBi | HostDB:ENSBTAG00000008540 |
VGNCi | VGNC:34719, SLC22A1 |
Phylogenomic databases
eggNOGi | KOG0255, Eukaryota |
GeneTreei | ENSGT00940000162065 |
HOGENOMi | CLU_001265_33_5_1 |
InParanoidi | A7MBE0 |
OMAi | YLWFSCA |
OrthoDBi | 704438at2759 |
TreeFami | TF315847 |
Enzyme and pathway databases
Reactomei | R-BTA-112311, Neurotransmitter clearance R-BTA-181430, Norepinephrine Neurotransmitter Release Cycle R-BTA-2161517, Abacavir transmembrane transport R-BTA-442660, Na+/Cl- dependent neurotransmitter transporters R-BTA-549127, Organic cation transport |
Gene expression databases
Bgeei | ENSBTAG00000008540, Expressed in cortex of kidney and 22 other tissues |
Family and domain databases
Gene3Di | 1.20.1250.20, 1 hit |
InterProi | View protein in InterPro IPR020846, MFS_dom IPR005828, MFS_sugar_transport-like IPR036259, MFS_trans_sf IPR004749, Orgcat_transp/SVOP IPR005829, Sugar_transporter_CS |
Pfami | View protein in Pfam PF00083, Sugar_tr, 1 hit |
SUPFAMi | SSF103473, SSF103473, 1 hit |
TIGRFAMsi | TIGR00898, 2A0119, 1 hit |
PROSITEi | View protein in PROSITE PS50850, MFS, 1 hit PS00216, SUGAR_TRANSPORT_1, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | S22A1_BOVIN | |
Accessioni | A7MBE0Primary (citable) accession number: A7MBE0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 20, 2008 |
Last sequence update: | October 2, 2007 | |
Last modified: | May 25, 2022 | |
This is version 97 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families