UniProtKB - A7MB62 (ARP2_BOVIN)
Actin-related protein 2
ACTR2
Functioni
ATP-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility. Seems to contact the pointed end of the daughter actin filament. In podocytes, required for the formation of lamellipodia downstream of AVIL and PLCE1 regulation. In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA. The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs).
By similarityRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 160 – 162 | ATP2 Publications | 3 | |
Nucleotide bindingi | 214 – 218 | ATP2 Publications | 5 | |
Nucleotide bindingi | 305 – 310 | ATP2 Publications | 6 |
GO - Molecular functioni
- actin binding Source: UniProtKB-KW
- ATP binding Source: UniProtKB-KW
GO - Biological processi
- Arp2/3 complex-mediated actin nucleation Source: UniProtKB
- positive regulation of double-strand break repair via homologous recombination Source: UniProtKB
- positive regulation of lamellipodium assembly Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: UniProtKB
Keywordsi
Molecular function | Actin-binding |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Actin-related protein 2Alternative name(s): Actin-like protein 2 |
Gene namesi | Name:ACTR2 Synonyms:ARP2 |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Organism-specific databases
VEuPathDBi | HostDB:ENSBTAG00000009761 |
VGNCi | VGNC:25586, ACTR2 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Cytoskeleton
- cytoskeleton By similarity
Other locations
- Cell projection By similarity
Cytoskeleton
- Arp2/3 protein complex Source: UniProtKB
Cytosol
- cytosol Source: Reactome
Nucleus
- nucleus Source: UniProtKB
Other locations
- cell projection Source: UniProtKB-SubCell
- cytoplasm Source: UniProtKB
- site of double-strand break Source: UniProtKB
Keywords - Cellular componenti
Cell projection, Cytoplasm, Cytoskeleton, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000327246 | 1 – 394 | Actin-related protein 2Add BLAST | 394 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Modified residuei | 299 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 322 | N6-acetyllysineBy similarity | 1 |
Keywords - PTMi
AcetylationProteomic databases
PaxDbi | A7MB62 |
PeptideAtlasi | A7MB62 |
PRIDEi | A7MB62 |
Interactioni
Subunit structurei
Binary interactionsi
A7MB62
With | #Exp. | IntAct |
---|---|---|
WASL [Q95107] | 2 | EBI-6162748,EBI-6162776 |
GO - Molecular functioni
- actin binding Source: UniProtKB-KW
Protein-protein interaction databases
BioGRIDi | 195053, 3 interactors |
DIPi | DIP-29789N |
IntActi | A7MB62, 4 interactors |
STRINGi | 9913.ENSBTAP00000012872 |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | A7MB62 |
SMRi | A7MB62 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | A7MB62 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0677, Eukaryota |
GeneTreei | ENSGT00940000154556 |
InParanoidi | A7MB62 |
OMAi | WEDMQHL |
OrthoDBi | 649708at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR004000, Actin IPR020902, Actin/actin-like_CS IPR043129, ATPase_NBD |
PANTHERi | PTHR11937, PTHR11937, 1 hit |
Pfami | View protein in Pfam PF00022, Actin, 1 hit |
PRINTSi | PR00190, ACTIN |
SMARTi | View protein in SMART SM00268, ACTIN, 1 hit |
SUPFAMi | SSF53067, SSF53067, 2 hits |
PROSITEi | View protein in PROSITE PS01132, ACTINS_ACT_LIKE, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MDSQGRKVVV CDNGTGFVKC GYAGSNFPEH IFPALVGRPI IRSTTKVGNI
60 70 80 90 100
EIKDLMVGDE ASELRSMLEV NYPMENGIVR NWDDMKHLWD YTFGPEKLNI
110 120 130 140 150
DTRNCKILLT EPPMNPTKNR EKIVEVMFET YQFSGVYVAI QAVLTLYAQG
160 170 180 190 200
LLTGVVVDSG DGVTHICPVY EGFSLPHLTR RLDIAGRDIT RYLIKLLLLR
210 220 230 240 250
GYAFNHSADF ETVRMIKEKL CYVGYNIEQE QKLALETTVL VESYTLPDGR
260 270 280 290 300
IIKVGGERFE APEALFQPHL INVEGVGVAE LLFNTIQAAD IDTRSEFYKH
310 320 330 340 350
IVLSGGSTMY PGLPSRLERE LKQLYLERVL KGDVEKLSKF KIRIEDPPRR
360 370 380 390
KHMVFLGGAV LADIMKDKDN FWMTRQEYQE KGVRVLEKLG VTVR
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A3Q1LXR9 | A0A3Q1LXR9_BOVIN | Actin-related protein 2 | ACTR2 | 387 | Annotation score: | ||
A0A3Q1M2H2 | A0A3Q1M2H2_BOVIN | Actin-related protein 2 | ACTR2 | 406 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC151356 mRNA Translation: AAI51357.1 |
RefSeqi | NP_001095683.1, NM_001102213.1 |
Genome annotation databases
Ensembli | ENSBTAT00000012872; ENSBTAP00000012872; ENSBTAG00000009761 |
GeneIDi | 538486 |
KEGGi | bta:538486 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC151356 mRNA Translation: AAI51357.1 |
RefSeqi | NP_001095683.1, NM_001102213.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1K8K | X-ray | 2.00 | B | 1-394 | [»] | |
1TYQ | X-ray | 2.55 | B | 1-394 | [»] | |
1U2V | X-ray | 2.55 | B | 1-394 | [»] | |
2P9I | X-ray | 2.46 | B | 1-394 | [»] | |
2P9K | X-ray | 2.59 | B | 1-394 | [»] | |
2P9L | X-ray | 2.65 | B | 1-394 | [»] | |
2P9N | X-ray | 2.85 | B | 1-394 | [»] | |
2P9P | X-ray | 2.90 | B | 1-394 | [»] | |
2P9S | X-ray | 2.68 | B | 1-394 | [»] | |
2P9U | X-ray | 2.75 | B | 1-394 | [»] | |
3DXK | X-ray | 2.70 | B | 1-394 | [»] | |
3DXM | X-ray | 2.85 | B | 1-394 | [»] | |
3RSE | X-ray | 2.65 | B | 1-394 | [»] | |
3UKR | X-ray | 2.48 | B | 1-394 | [»] | |
3UKU | X-ray | 2.75 | B | 1-394 | [»] | |
3ULE | X-ray | 2.50 | B | 1-394 | [»] | |
4JD2 | X-ray | 3.08 | B | 1-394 | [»] | |
4XEI | X-ray | 3.87 | B | 1-394 | [»] | |
4XF2 | X-ray | 5.00 | B/U | 1-394 | [»] | |
6DEC | X-ray | 4.60 | B/I | 1-394 | [»] | |
AlphaFoldDBi | A7MB62 | |||||
SMRi | A7MB62 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 195053, 3 interactors |
DIPi | DIP-29789N |
IntActi | A7MB62, 4 interactors |
STRINGi | 9913.ENSBTAP00000012872 |
Proteomic databases
PaxDbi | A7MB62 |
PeptideAtlasi | A7MB62 |
PRIDEi | A7MB62 |
Genome annotation databases
Ensembli | ENSBTAT00000012872; ENSBTAP00000012872; ENSBTAG00000009761 |
GeneIDi | 538486 |
KEGGi | bta:538486 |
Organism-specific databases
CTDi | 10097 |
VEuPathDBi | HostDB:ENSBTAG00000009761 |
VGNCi | VGNC:25586, ACTR2 |
Phylogenomic databases
eggNOGi | KOG0677, Eukaryota |
GeneTreei | ENSGT00940000154556 |
InParanoidi | A7MB62 |
OMAi | WEDMQHL |
OrthoDBi | 649708at2759 |
Miscellaneous databases
EvolutionaryTracei | A7MB62 |
PROi | PR:A7MB62 |
Gene expression databases
ExpressionAtlasi | A7MB62, baseline and differential |
Family and domain databases
InterProi | View protein in InterPro IPR004000, Actin IPR020902, Actin/actin-like_CS IPR043129, ATPase_NBD |
PANTHERi | PTHR11937, PTHR11937, 1 hit |
Pfami | View protein in Pfam PF00022, Actin, 1 hit |
PRINTSi | PR00190, ACTIN |
SMARTi | View protein in SMART SM00268, ACTIN, 1 hit |
SUPFAMi | SSF53067, SSF53067, 2 hits |
PROSITEi | View protein in PROSITE PS01132, ACTINS_ACT_LIKE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ARP2_BOVIN | |
Accessioni | A7MB62Primary (citable) accession number: A7MB62 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 8, 2008 |
Last sequence update: | October 2, 2007 | |
Last modified: | May 25, 2022 | |
This is version 92 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families