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UniProtKB - A7LXS9 (BGH2A_BACO1)
Protein
Beta-galactosidase BoGH2A
Gene
BACOVA_02645
Organism
Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153)
Status
Functioni
Catalyzes the hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides in xyloglucan degradation, converting 'L' units to 'X' units.
1 PublicationMiscellaneous
Gut bacteria supply the human body with energy from dietary polysaccharides through glycosidases that are absent in the human genome. Xyloglucans are a ubiquitous family of highly branched plant cell wall polysaccharides present in the vegetables we consume. Enzymes involved in xyloglucan degradation mediate the conversion of otherwise indigestible plant polysaccharides to short-chain fatty acids (PubMed:24463512).1 Publication
Catalytic activityi
- Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.1 Publication EC:3.2.1.23
Kineticsi
kcat is 4.0 sec(-1) for Gal-beta-PNP.
- KM=0.087 mM for Gal-beta-PNP1 Publication
pH dependencei
Optimum pH is 6.0-7.0.1 Publication
: xyloglucan degradation Pathwayi
This protein is involved in the pathway xyloglucan degradation, which is part of Glucan metabolism.1 PublicationView all proteins of this organism that are known to be involved in the pathway xyloglucan degradation and in Glucan metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 437 | Proton donorBy similarity | 1 | |
Active sitei | 544 | NucleophileBy similarity | 1 |
GO - Molecular functioni
- beta-galactosidase activity Source: UniProtKB
GO - Biological processi
- symbiotic process benefiting host Source: UniProtKB
- xyloglucan catabolic process Source: UniProtKB
Keywordsi
Molecular function | Glycosidase, Hydrolase |
Biological process | Carbohydrate metabolism, Polysaccharide degradation |
Enzyme and pathway databases
SABIO-RKi | A7LXS9 |
UniPathwayi | UPA01045 |
Protein family/group databases
CAZyi | GH2, Glycoside Hydrolase Family 2 |
Names & Taxonomyi
Protein namesi | Recommended name: Beta-galactosidase BoGH2A (EC:3.2.1.23)Short name: Beta-gal Alternative name(s): Glycosyl hydrolase family protein 2A Short name: BoGH2A |
Gene namesi | ORF Names:BACOVA_02645 |
Organismi | Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) |
Taxonomic identifieri | 411476 [NCBI] |
Taxonomic lineagei | Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane Curated; Lipid-anchor Curated
Note: Cell inner membrane localization is predicted by analogy with the archetypal sus locus.1 Publication
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 19 | Sequence analysisAdd BLAST | 19 | |
ChainiPRO_0000425891 | 20 – 851 | Beta-galactosidase BoGH2AAdd BLAST | 832 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 20 | N-palmitoyl cysteineSequence analysis | 1 | |
Lipidationi | 20 | S-diacylglycerol cysteineSequence analysis | 1 |
Keywords - PTMi
Lipoprotein, PalmitateProteomic databases
PRIDEi | A7LXS9 |
Family & Domainsi
Sequence similaritiesi
Belongs to the glycosyl hydrolase 2 family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | COG3250, Bacteria |
HOGENOMi | CLU_006501_0_1_10 |
Family and domain databases
Gene3Di | 2.60.40.10, 3 hits |
InterProi | View protein in InterPro IPR036156, Beta-gal/glucu_dom_sf IPR032311, DUF4982 IPR008979, Galactose-bd-like_sf IPR040605, Glyco_hydro2_dom5 IPR006101, Glyco_hydro_2 IPR023232, Glyco_hydro_2_AS IPR006103, Glyco_hydro_2_cat IPR006102, Glyco_hydro_2_Ig-like IPR006104, Glyco_hydro_2_N IPR017853, Glycoside_hydrolase_SF IPR013783, Ig-like_fold IPR008964, Invasin/intimin_cell_adhesion |
Pfami | View protein in Pfam PF16355, DUF4982, 1 hit PF18565, Glyco_hydro2_C5, 1 hit PF00703, Glyco_hydro_2, 1 hit PF02836, Glyco_hydro_2_C, 1 hit PF02837, Glyco_hydro_2_N, 1 hit |
PRINTSi | PR00132, GLHYDRLASE2 |
SUPFAMi | SSF49303, SSF49303, 1 hit SSF49373, SSF49373, 1 hit SSF49785, SSF49785, 1 hit SSF51445, SSF51445, 1 hit |
PROSITEi | View protein in PROSITE PS00608, GLYCOSYL_HYDROL_F2_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
A7LXS9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MMIGKLKYLM LGGCLILGSC LALGGCLMLL GACSSSSLVS PRERSDFNAD
60 70 80 90 100
WRFHLGDGLQ AAQPGFADND WRVLDLPHDW AIEGDFSQEN PSGTGGGALP
110 120 130 140 150
GGVGWYRKTF SVDKADAGKI FRIEFDGVYM NSEVFINGVS LGVRPYGYIS
160 170 180 190 200
FSYDLTPYLK WDEPNVLAVR VDNAEQPNSR WYSGCGIYRN VWLSKTGPIH
210 220 230 240 250
VGGWGTYVTT SSVDEKQAVL NLATTLVNES DTNENVTVCS SLQDAEGREV
260 270 280 290 300
AETRSSGEAE AGKEVVFTQQ LTVKQPQLWD IDTPYLYTLV TKVMRNEECM
310 320 330 340 350
DRYTTPVGIR TFSLDARKGF TLNGRQTKIN GVCMHHDLGC LGAAVNTRAI
360 370 380 390 400
ERHLQILKEM GCNGIRCSHN PPAPELLDLC DRMGFIVMDE AFDMWRKKKT
410 420 430 440 450
AHDYARYFNE WHERDLNDFI LRDRNHPSVF MWSIGNEVLE QWSDAKADTL
460 470 480 490 500
SLEEANLILN FGHSSEMLAK EGEESVNSLL TKKLVSFVKG LDPTRPVTAG
510 520 530 540 550
CNEPNSGNHL FRSGVLDVIG YNYHNKDIPN VPANFPDKPF IITESNSALM
560 570 580 590 600
TRGYYRMPSD RMFIWPKRWD KSFADSTFAC SSYENCHVPW GNTHEESLKL
610 620 630 640 650
VRDNDFISGQ YVWTGFDYIG EPTPYGWPAR SSYFGIVDLA GFPKDVYYLY
660 670 680 690 700
QSEWTDKQVL HLFPHWNWTP GQEIDMWCYY NQADEVELFV NGKSQGVKRK
710 720 730 740 750
DLDNLHVAWR VKFEPGTVKV IARESGKVVA EKEICTAGKP AEIRLTPDRS
760 770 780 790 800
ILTADGKDLC FVTVEVLDEK GNLCPDADNL VNFTVQGNGF IAGVDNGNPV
810 820 830 840 850
SMERFKDEKR KAFYGKCLVV IQNDGKPGKA KLTATSEGLR QAVLKISAEE
L
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AAXF02000049 Genomic DNA Translation: EDO11436.1 |
Genome annotation databases
EnsemblBacteriai | EDO11436; EDO11436; BACOVA_02645 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AAXF02000049 Genomic DNA Translation: EDO11436.1 |
3D structure databases
AlphaFoldDBi | A7LXS9 |
SMRi | A7LXS9 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 411476.BACOVA_02645 |
Protein family/group databases
CAZyi | GH2, Glycoside Hydrolase Family 2 |
Proteomic databases
PRIDEi | A7LXS9 |
Genome annotation databases
EnsemblBacteriai | EDO11436; EDO11436; BACOVA_02645 |
Phylogenomic databases
eggNOGi | COG3250, Bacteria |
HOGENOMi | CLU_006501_0_1_10 |
Enzyme and pathway databases
UniPathwayi | UPA01045 |
SABIO-RKi | A7LXS9 |
Family and domain databases
Gene3Di | 2.60.40.10, 3 hits |
InterProi | View protein in InterPro IPR036156, Beta-gal/glucu_dom_sf IPR032311, DUF4982 IPR008979, Galactose-bd-like_sf IPR040605, Glyco_hydro2_dom5 IPR006101, Glyco_hydro_2 IPR023232, Glyco_hydro_2_AS IPR006103, Glyco_hydro_2_cat IPR006102, Glyco_hydro_2_Ig-like IPR006104, Glyco_hydro_2_N IPR017853, Glycoside_hydrolase_SF IPR013783, Ig-like_fold IPR008964, Invasin/intimin_cell_adhesion |
Pfami | View protein in Pfam PF16355, DUF4982, 1 hit PF18565, Glyco_hydro2_C5, 1 hit PF00703, Glyco_hydro_2, 1 hit PF02836, Glyco_hydro_2_C, 1 hit PF02837, Glyco_hydro_2_N, 1 hit |
PRINTSi | PR00132, GLHYDRLASE2 |
SUPFAMi | SSF49303, SSF49303, 1 hit SSF49373, SSF49373, 1 hit SSF49785, SSF49785, 1 hit SSF51445, SSF51445, 1 hit |
PROSITEi | View protein in PROSITE PS00608, GLYCOSYL_HYDROL_F2_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | BGH2A_BACO1 | |
Accessioni | A7LXS9Primary (citable) accession number: A7LXS9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 19, 2014 |
Last sequence update: | October 2, 2007 | |
Last modified: | May 25, 2022 | |
This is version 68 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Documents
- Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families