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Protein

S-methyl-5'-thioadenosine phosphorylase

Gene

SS1G_02233

Organism
Sclerotinia sclerotiorum (strain ATCC 18683 / 1980 / Ss-1) (White mold) (Whetzelinia sclerotiorum)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.UniRule annotation

Catalytic activityi

S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate.UniRule annotation

Pathwayi: L-methionine biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (phosphorylase route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. S-methyl-5'-thioadenosine phosphorylase (SS1G_02233), S-methyl-5'-thioadenosine phosphorylase (sscle_04g033010)
This subpathway is part of the pathway L-methionine biosynthesis via salvage pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (phosphorylase route), the pathway L-methionine biosynthesis via salvage pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei21PhosphateUniRule annotation1
Sitei180Important for substrate specificityUniRule annotation1
Binding sitei198Substrate; via amide nitrogenUniRule annotation1
Binding sitei199PhosphateUniRule annotation1
Sitei234Important for substrate specificityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processPurine salvage

Enzyme and pathway databases

UniPathwayi
UPA00904;UER00873

Names & Taxonomyi

Protein namesi
Recommended name:
S-methyl-5'-thioadenosine phosphorylaseUniRule annotation (EC:2.4.2.28UniRule annotation)
Alternative name(s):
5'-methylthioadenosine phosphorylaseUniRule annotation
Short name:
MTA phosphorylaseUniRule annotation
Short name:
MTAPUniRule annotation
Short name:
MTAPaseUniRule annotation
Gene namesi
ORF Names:SS1G_02233
OrganismiSclerotinia sclerotiorum (strain ATCC 18683 / 1980 / Ss-1) (White mold) (Whetzelinia sclerotiorum)
Taxonomic identifieri665079 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaLeotiomycetesHelotialesSclerotiniaceaeSclerotinia
Proteomesi
  • UP000001312 Componenti: Unassembled WGS sequence

Organism-specific databases

EuPathDBiFungiDB:SS1G_02233

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004151351 – 306S-methyl-5'-thioadenosine phosphorylaseAdd BLAST306

Interactioni

Subunit structurei

Homotrimer.UniRule annotation

Protein-protein interaction databases

STRINGi5180.EDN99379

Structurei

3D structure databases

ProteinModelPortaliA7EAA1
SMRiA7EAA1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni63 – 64Phosphate bindingUniRule annotation2
Regioni96 – 97Phosphate bindingUniRule annotation2
Regioni222 – 224Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.UniRule annotation

Phylogenomic databases

InParanoidiA7EAA1
KOiK00772
OMAiNLYRSWG
OrthoDBiEOG092C46SL

Family and domain databases

HAMAPiMF_01963 MTAP, 1 hit
InterProiView protein in InterPro
IPR010044 MTAP
IPR000845 Nucleoside_phosphorylase_d
IPR035994 Nucleoside_phosphorylase_sf
IPR018099 Purine_phosphorylase-2_CS
PANTHERiPTHR42679 PTHR42679, 1 hit
PfamiView protein in Pfam
PF01048 PNP_UDP_1, 1 hit
SUPFAMiSSF53167 SSF53167, 1 hit
TIGRFAMsiTIGR01694 MTAP, 1 hit
PROSITEiView protein in PROSITE
PS01240 PNP_MTAP_2, 1 hit

Sequencei

Sequence statusi: Complete.

A7EAA1-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MVESLPTTFS DSVHIAVIGG TGLQSLEGFI PIATINPLTP WGYPSAPIHI
60 70 80 90 100
LSHNNTPIAF LSRHGTHHEL APHEIPNRAN IAALRSMGVR TIIAFSAVGS
110 120 130 140 150
LREEIKPRDF VVPDQVIDRT KGVRPFTFFE KGVVGHVGFA DPFDERIAKV
160 170 180 190 200
VRECGHALEG EGIVLHDKGT IICMEGPAFS TRAESHMYRS WGGSVINMSA
210 220 230 240 250
LPEAKLAREA EMVYQMICMA TDYDCWHSTA DVDVEMVMGH MHANGQNAKR
260 270 280 290 300
LVGAVLDALN MRWSTIDSIR RRKNKPELAN AQQHQIWRGR TYHFAWSISA

CYFSSE
Length:306
Mass (Da):33,972
Last modified:September 11, 2007 - v1
Checksum:i12F799D117340BF0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH476623 Genomic DNA Translation: EDN99379.1
RefSeqiXP_001596017.1, XM_001595967.1

Genome annotation databases

EnsemblFungiiEDN99379; EDN99379; SS1G_02233
GeneIDi5492464
KEGGissl:SS1G_02233

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH476623 Genomic DNA Translation: EDN99379.1
RefSeqiXP_001596017.1, XM_001595967.1

3D structure databases

ProteinModelPortaliA7EAA1
SMRiA7EAA1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi5180.EDN99379

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiEDN99379; EDN99379; SS1G_02233
GeneIDi5492464
KEGGissl:SS1G_02233

Organism-specific databases

EuPathDBiFungiDB:SS1G_02233

Phylogenomic databases

InParanoidiA7EAA1
KOiK00772
OMAiNLYRSWG
OrthoDBiEOG092C46SL

Enzyme and pathway databases

UniPathwayi
UPA00904;UER00873

Family and domain databases

HAMAPiMF_01963 MTAP, 1 hit
InterProiView protein in InterPro
IPR010044 MTAP
IPR000845 Nucleoside_phosphorylase_d
IPR035994 Nucleoside_phosphorylase_sf
IPR018099 Purine_phosphorylase-2_CS
PANTHERiPTHR42679 PTHR42679, 1 hit
PfamiView protein in Pfam
PF01048 PNP_UDP_1, 1 hit
SUPFAMiSSF53167 SSF53167, 1 hit
TIGRFAMsiTIGR01694 MTAP, 1 hit
PROSITEiView protein in PROSITE
PS01240 PNP_MTAP_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMTAP_SCLS1
AccessioniPrimary (citable) accession number: A7EAA1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: September 11, 2007
Last modified: October 10, 2018
This is version 61 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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