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Protein

Neutrophil cytosol factor 2

Gene

Ncf2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).By similarity

GO - Molecular functioni

GO - Biological processi

  • aging Source: RGD
  • cellular response to hormone stimulus Source: RGD
  • phagocytosis Source: InterPro
  • positive regulation of blood pressure Source: RGD
  • positive regulation of neuron apoptotic process Source: RGD
  • respiratory burst Source: InterPro
  • response to activity Source: RGD
  • response to drug Source: RGD
  • response to glucose Source: RGD
  • response to hyperoxia Source: RGD
  • response to laminar fluid shear stress Source: RGD
  • response to lipid Source: RGD
  • response to lipopolysaccharide Source: RGD
  • response to organic cyclic compound Source: RGD
  • response to progesterone Source: RGD

Names & Taxonomyi

Protein namesi
Recommended name:
Neutrophil cytosol factor 2
Short name:
NCF-2
Alternative name(s):
Neutrophil NADPH oxidase factor 2
Gene namesi
Name:Ncf2Imported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1309424 Ncf2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003122281 – 527Neutrophil cytosol factor 2Add BLAST527

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei225PhosphothreonineBy similarity1
Modified residuei316PhosphoserineBy similarity1
Modified residuei400PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiA7E3N2
PeptideAtlasiA7E3N2
PRIDEiA7E3N2

PTM databases

iPTMnetiA7E3N2
PhosphoSitePlusiA7E3N2

Interactioni

Subunit structurei

Component of an NADPH oxidase complex composed of a heterodimer formed by the membrane proteins CYBA and CYBB and the cytosolic subunits NCF1, NCF2 and NCF4. Interacts with NCF4. Interacts (via the C-terminal SH3 domain) with NCF1 (via C-terminus). Interacts with SYTL1 and RAC1. May interact with NOXO1. Interacts with S100A8 and calprotectin (S100A8/9) (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000060733

Structurei

3D structure databases

ProteinModelPortaliA7E3N2
SMRiA7E3N2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati37 – 70TPR 1Sequence analysisAdd BLAST34
Repeati72 – 104TPR 2Sequence analysisAdd BLAST33
Repeati119 – 152TPR 3Sequence analysisAdd BLAST34
Domaini232 – 291SH3 1PROSITE-ProRule annotationAdd BLAST60
Domaini352 – 430PB1PROSITE-ProRule annotationAdd BLAST79
Domaini458 – 517SH3 2PROSITE-ProRule annotationAdd BLAST60

Domaini

The OPR/PB1 domain mediates the association with NCF4/p40-PHOX.By similarity

Sequence similaritiesi

Belongs to the NCF2/NOXA1 family.Curated

Keywords - Domaini

Repeat, SH3 domain, TPR repeat

Phylogenomic databases

eggNOGiKOG4225 Eukaryota
ENOG41110AD LUCA
HOGENOMiHOG000237312
HOVERGENiHBG001521
InParanoidiA7E3N2
KOiK08010
OrthoDBiEOG091G04T7
PhylomeDBiA7E3N2
TreeFamiTF329087

Family and domain databases

CDDicd06406 PB1_P67, 1 hit
cd11871 SH3_p67phox_N, 1 hit
Gene3Di1.25.40.10, 1 hit
InterProiView protein in InterPro
IPR034889 P67-PHOX
IPR035546 p67phox_SH3_1
IPR000270 PB1_dom
IPR034885 PB1_P67
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
IPR013026 TPR-contain_dom
IPR011990 TPR-like_helical_dom_sf
IPR019734 TPR_repeat
PANTHERiPTHR15175:SF3 PTHR15175:SF3, 1 hit
PfamiView protein in Pfam
PF00564 PB1, 1 hit
PF00018 SH3_1, 2 hits
PF13181 TPR_8, 1 hit
SMARTiView protein in SMART
SM00666 PB1, 1 hit
SM00326 SH3, 2 hits
SM00028 TPR, 3 hits
SUPFAMiSSF48452 SSF48452, 1 hit
SSF50044 SSF50044, 2 hits
PROSITEiView protein in PROSITE
PS51745 PB1, 1 hit
PS50002 SH3, 2 hits
PS50293 TPR_REGION, 1 hit

Sequencei

Sequence statusi: Complete.

A7E3N2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLAEAIRLW NEGVQAADKK DWKGALEAFS EVQDPHSRIC FNIGCMYTIL
60 70 80 90 100
DNLQEAEQAF TKSINRDKHL AVAYFQRGML YYSMEKYRPA SVGRKAALLF
110 120 130 140 150
LGSYNLVARI IVGYPLSPGK VLYNIALMHA KKEEWKKAEE QLALATNMKS
160 170 180 190 200
EPRHSKIDKA MESIWKRCPT SHLPLDPPQV TMALWFEEGG VGKRSVVASV
210 220 230 240 250
VHQDNFSGFA PLQPQSAEPP PRPKTPEIFR ALEGEAHRVL FGFVPETPEE
260 270 280 290 300
LQVMPGNIVF VLKKGSDNWA TVMFNGQKGL VPCNYLEPVE LRIHPQSQPQ
310 320 330 340 350
EDTSLESDIP PPPNSSPPER LQLSPGWCQQ LGPLRCPPFL LHQEVKRSVP
360 370 380 390 400
MPYMLKVHYK YTVVMETQLG LPYSQLRNMV SKKLELLPEH TKLSYQRRDS
410 420 430 440 450
PELLLLSEES MKDAWAQVKN YCLTLWCEHT VGDQGFVDEP KEKENSDADN
460 470 480 490 500
RTTEPQPKEG TQVVAIFSYD ATQPEDLEFV EGDVILVLSH VNEEWLEGEC
510 520
KGKIGIFPKA FVEGCAAKNL EGTPREV
Length:527
Mass (Da):59,573
Last modified:September 11, 2007 - v1
Checksum:iE025D50A0BA222E5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03084702 Genomic DNA No translation available.
AABR03085295 Genomic DNA No translation available.
AABR03088578 Genomic DNA No translation available.
BR000294 mRNA Translation: FAA00361.1
RefSeqiNP_001094454.1, NM_001100984.1
UniGeneiRn.162331

Genome annotation databases

GeneIDi364018
KEGGirno:364018
UCSCiRGD:1309424 rat

Similar proteinsi

Entry informationi

Entry nameiNCF2_RAT
AccessioniPrimary (citable) accession number: A7E3N2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: September 11, 2007
Last modified: May 23, 2018
This is version 83 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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