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Protein

NADH-cytochrome b5 reductase 1

Gene

CBR1

Organism
Saccharomyces cerevisiae (strain YJM789) (Baker's yeast)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Electron donor reductase for cytochrome b5. The cytochrome b5/NADH cytochrome b5 reductase electron transfer system supports the catalytic activity of several sterol biosynthetic enzymes. Plays a role in bud morphology (By similarity).By similarity

Catalytic activityi

NADH + 2 ferricytochrome b5 = NAD+ + H+ + 2 ferrocytochrome b5.

Cofactori

FADBy similarity

Enzyme regulationi

Competitively inhibited by NAD+. Inhibited by mercurials such as p-chloromercuribenzoate (PCMB) and HgCl2. Enzymatic activity increases under anaerobic conditions (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi148 – 180FADBy similarityAdd BLAST33

GO - Molecular functioni

Keywordsi

Molecular functionOxidoreductase
LigandFAD, Flavoprotein, NAD

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-cytochrome b5 reductase 1 (EC:1.6.2.2)
Alternative name(s):
Microsomal cytochrome b reductase
P35
Gene namesi
Name:CBR1
ORF Names:SCY_2742
OrganismiSaccharomyces cerevisiae (strain YJM789) (Baker's yeast)
Taxonomic identifieri307796 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000007060 Componenti: Unassembled WGS sequence

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei7 – 27HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003301671 – 284NADH-cytochrome b5 reductase 1Add BLAST284

Proteomic databases

PRIDEiA6ZVM6
TopDownProteomicsiA6ZVM6

Expressioni

Inductioni

Protein levels are highest during exponential growth phase and lowest in stationary phase.By similarity

Interactioni

Subunit structurei

Monomer. Interacts with STE20.By similarity

Structurei

3D structure databases

ProteinModelPortaliA6ZVM6
SMRiA6ZVM6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini38 – 142FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST105

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

OrthoDBiEOG092C43G8

Family and domain databases

Gene3Di3.40.50.80, 1 hit
InterProiView protein in InterPro
IPR017927 Fd_Rdtase_FAD-bd
IPR001709 Flavoprot_Pyr_Nucl_cyt_Rdtase
IPR039261 FNR_nucleotide_bd
IPR001834 NADH-Cyt_B5_reductase
IPR008333 OxRdtase_FAD-bd_dom
IPR001433 OxRdtase_FAD/NAD-bd
IPR017938 Riboflavin_synthase-like_b-brl
PfamiView protein in Pfam
PF00970 FAD_binding_6, 1 hit
PF00175 NAD_binding_1, 1 hit
PRINTSiPR00406 CYTB5RDTASE
PR00371 FPNCR
SUPFAMiSSF52343 SSF52343, 1 hit
SSF63380 SSF63380, 1 hit
PROSITEiView protein in PROSITE
PS51384 FAD_FR, 1 hit

Sequencei

Sequence statusi: Complete.

A6ZVM6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAIDAQKLVV VIVIVVVPLL FKFIIGPKTK PVLDPKRNDF QSFPLVEKTI
60 70 80 90 100
LTHNTSMYKF GLPHADDVLG LPIGQHIVIK ANINGKDITR SYTPTSLDGD
110 120 130 140 150
TKGNFELLVK SYPTGNVSKM IGELKIGDSI QIKGPRGNYH YERNCRSHLG
160 170 180 190 200
MIAGGTGIAP MYQIMKAIAM DSHDTTKVSL VFGNVHEEDI LLKKELEALV
210 220 230 240 250
AMKPSQFKIV YYLDSPDRED WAGGVGYITK DVIKEHLPAA TVDNVQILIC
260 270 280
GPPAMVASVR RSTVDLGFRR SKPLSKMEDQ VFVF
Length:284
Mass (Da):31,422
Last modified:April 29, 2008 - v2
Checksum:iCF24B16DD34A7A12
GO

Sequence cautioni

The sequence EDN61451 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFW02000124 Genomic DNA Translation: EDN61451.1 Different initiation.

Genome annotation databases

EnsemblFungiiEDN61451; EDN61451; SCY_2742

Similar proteinsi

Entry informationi

Entry nameiNCB5R_YEAS7
AccessioniPrimary (citable) accession number: A6ZVM6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: April 29, 2008
Last modified: July 18, 2018
This is version 50 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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