Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Oxamate amidohydrolase proenzyme

Gene

hpxW

Organism
Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the uric acid degradation pathway. Catalyzes the conversion of oxamate to oxalate.1 Publication

Catalytic activityi

Oxamate + H2O = oxalate + ammonia.1 Publication

Kineticsi

Kcat is 5.5 sec(-1) with oxamate as substrate.1 Publication

      Sites

      Feature keyPosition(s)DescriptionActionsGraphical viewLength
      Active sitei342Nucleophile1 Publication1
      Sitei360Important for catalytic activity1 Publication1

      GO - Molecular functioni

      Keywordsi

      Molecular functionHydrolase, Protease

      Enzyme and pathway databases

      BioCyciKPNE272620:G1G8J-1900-MONOMER

      Protein family/group databases

      MEROPSiT03.A09

      Names & Taxonomyi

      Protein namesi
      Recommended name:
      Oxamate amidohydrolase proenzyme1 Publication (EC:3.5.1.1261 Publication)
      Cleaved into the following 2 chains:
      Alternative name(s):
      Oxamate amidohydrolase alpha chain1 Publication
      Alternative name(s):
      Oxamate amidohydrolase beta chain1 Publication
      Gene namesi
      Name:hpxW1 Publication
      ORF Names:KPN_01768Imported
      OrganismiKlebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)
      Taxonomic identifieri272620 [NCBI]
      Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeKlebsiella
      Proteomesi
      • UP000000265 Componenti: Chromosome

      Pathology & Biotechi

      Mutagenesis

      Feature keyPosition(s)DescriptionActionsGraphical viewLength
      Mutagenesisi342T → A: Unable to autoprocess into a mature heterodimer. Lack of amidohydrolase activity. 1 Publication1
      Mutagenesisi360S → A: Forms a heterodimer, however lacks amidohydrolase activity. 1 Publication1

      PTM / Processingi

      Molecule processing

      Feature keyPosition(s)DescriptionActionsGraphical viewLength
      ChainiPRO_00004427671 – 341Oxamate amidohydrolase large chainAdd BLAST341
      ChainiPRO_0000442768342 – 528Oxamate amidohydrolase small chainAdd BLAST187

      Post-translational modificationi

      Cleaved by autocatalysis into a large (alpha) and a small (beta) subunit.1 Publication

      Keywords - PTMi

      Zymogen

      Interactioni

      Subunit structurei

      Heterodimer that consists of a 35.5 kDa large (alpha) subunit and a 20 kDa small (beta) subunit, which are synthesized from a single polypeptide.1 Publication

      Protein-protein interaction databases

      STRINGi272620.KPN_01768

      Structurei

      Secondary structure

      1528
      Legend: HelixTurnBeta strandPDB Structure known for this area
      Show more details

      3D structure databases

      ProteinModelPortaliA6T9C8
      SMRiA6T9C8
      ModBaseiSearch...
      MobiDBiSearch...

      Family & Domainsi

      Region

      Feature keyPosition(s)DescriptionActionsGraphical viewLength
      Regioni424 – 425Substrate binding1 Publication2

      Sequence similaritiesi

      Belongs to the gamma-glutamyltransferase family.Curated

      Phylogenomic databases

      eggNOGiENOG4105CFB Bacteria
      COG0405 LUCA
      HOGENOMiHOG000175618
      KOiK22602
      OMAiGRTWGQS

      Family and domain databases

      InterProiView protein in InterPro
      IPR029055 Ntn_hydrolases_N
      SUPFAMiSSF56235 SSF56235, 1 hit

      Sequencei

      Sequence statusi: Complete.

      Sequence processingi: The displayed sequence is further processed into a mature form.

      A6T9C8-1 [UniParc]FASTAAdd to basket
      « Hide
              10         20         30         40         50
      MHSSNVSTHG MAVAPHHLAS QSALAILREG GSAIEAMVAA AAAIAVVYPH
      60 70 80 90 100
      MNGLGGDGFW LIVPPEGDPI AIDASGAAGS LATLEAYAGQ RHIPNRGPQA
      110 120 130 140 150
      ALTVAGTVSG WVEALRISRD LTGRALPVAR LLADAIGYAE DGIPVTASQA
      160 170 180 190 200
      HATASKLEEL RHQPGFSETW LVAGEAPRPG SRFRQPALAG TLRMLASDGL
      210 220 230 240 250
      DSFYRGPLAE RLAQGMAALG MPITLGDLQA HRARRPGPLT LQHQQGTLWN
      260 270 280 290 300
      LAPPTQGLVS LAILGITDRL KMADADDAQT VHRIVEATKR AFALRDAHIT
      310 320 330 340 350
      DPRHLDVDVQ QLLTPEALQP LADSIDDASA SPWGGGKGPG DTVWMGVVDN
      360 370 380 390 400
      SGLAVSFIQS IYHEFGSGVV LPDTGIVWQN RGAAFSLDPQ HLLALAPGKQ
      410 420 430 440 450
      PFHTLNPAAA RLNDGRVMVY GSMGGDGQPQ TQAALFTRYI LQGVPLQESI
      460 470 480 490 500
      SRPRWLLGRT WGQSSDSLKL EGRFAPACIA RLRELGHDVE VLADFSEAMG
      510 520
      HAGAIVRHPN GLLEGATDPR SNGAAAGY
      Length:528
      Mass (Da):55,542
      Last modified:August 21, 2007 - v1
      Checksum:i5375362CBACF6316
      GO

      Sequence databases

      Select the link destinations:
      EMBLi
      GenBanki
      DDBJi
      Links Updated
      CP000647 Genomic DNA Translation: ABR77199.1
      RefSeqiWP_015958435.1, NC_009648.1

      Genome annotation databases

      EnsemblBacteriaiABR77199; ABR77199; KPN_01768
      KEGGikpn:KPN_01768

      Similar proteinsi

      Cross-referencesi

      Sequence databases

      Select the link destinations:
      EMBLi
      GenBanki
      DDBJi
      Links Updated
      CP000647 Genomic DNA Translation: ABR77199.1
      RefSeqiWP_015958435.1, NC_009648.1

      3D structure databases

      Select the link destinations:
      PDBei
      RCSB PDBi
      PDBji
      Links Updated
      PDB entryMethodResolution (Å)ChainPositionsPDBsum
      5HFTX-ray2.65A/C1-341[»]
      B/D342-528[»]
      ProteinModelPortaliA6T9C8
      SMRiA6T9C8
      ModBaseiSearch...
      MobiDBiSearch...

      Protein-protein interaction databases

      STRINGi272620.KPN_01768

      Protein family/group databases

      MEROPSiT03.A09

      Protocols and materials databases

      Structural Biology KnowledgebaseSearch...

      Genome annotation databases

      EnsemblBacteriaiABR77199; ABR77199; KPN_01768
      KEGGikpn:KPN_01768

      Phylogenomic databases

      eggNOGiENOG4105CFB Bacteria
      COG0405 LUCA
      HOGENOMiHOG000175618
      KOiK22602
      OMAiGRTWGQS

      Enzyme and pathway databases

      BioCyciKPNE272620:G1G8J-1900-MONOMER

      Family and domain databases

      InterProiView protein in InterPro
      IPR029055 Ntn_hydrolases_N
      SUPFAMiSSF56235 SSF56235, 1 hit
      ProtoNetiSearch...

      Entry informationi

      Entry nameiHPXW_KLEP7
      AccessioniPrimary (citable) accession number: A6T9C8
      Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2017
      Last sequence update: August 21, 2007
      Last modified: June 20, 2018
      This is version 59 of the entry and version 1 of the sequence. See complete history.
      Entry statusiReviewed (UniProtKB/Swiss-Prot)
      Annotation programProkaryotic Protein Annotation Program

      Miscellaneousi

      Keywords - Technical termi

      3D-structure, Complete proteome, Reference proteome

      Documents

      1. SIMILARITY comments
        Index of protein domains and families
      2. PDB cross-references
        Index of Protein Data Bank (PDB) cross-references
      UniProt is an ELIXIR core data resource
      Main funding by: National Institutes of Health

      We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

      Do not show this banner again