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UniProtKB - A6T923 (HPXO_KLEP7)
Protein
FAD-dependent urate hydroxylase
Gene
hpxO
Organism
Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)
Status
Functioni
Catalyzes the hydroxylation of urate to 5-hydroxyisourate (HIU).
1 PublicationCatalytic activityi
- EC:1.14.13.1131 Publication
Cofactori
FAD1 Publication
Kineticsi
kcat is 42 sec(-1).1 Publication
- KM=42 µM for urate1 Publication
: urate degradation Pathwayi
This protein is involved in the pathway urate degradation, which is part of Purine metabolism.1 PublicationView all proteins of this organism that are known to be involved in the pathway urate degradation and in Purine metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 11 | FAD; via amide nitrogenCombined sources1 Publication | 1 | |
Binding sitei | 43 | FADCombined sources1 Publication | 1 | |
Binding sitei | 125 | FAD; via amide nitrogen and carbonyl oxygenCombined sources1 Publication | 1 | |
Binding sitei | 178 | SubstrateCombined sources1 Publication | 1 | |
Binding sitei | 204 | SubstrateCombined sources1 Publication | 1 | |
Sitei | 204 | Involved in substrate activation for the transfer of oxygen from the flavin hydroperoxide1 Publication | 1 | |
Binding sitei | 285 | FADCombined sources1 Publication | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 30 – 31 | FADCombined sources1 Publication | 2 | |
Nucleotide bindingi | 295 – 299 | FADCombined sources1 Publication | 5 |
GO - Molecular functioni
- FAD binding Source: UniProtKB
- FAD-dependent urate hydroxylase activity Source: UniProtKB-EC
- oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen Source: UniProtKB
- urate oxidase activity Source: CACAO
GO - Biological processi
- purine nucleobase metabolic process Source: UniProtKB-KW
- urate catabolic process Source: UniProtKB
Keywordsi
Molecular function | Monooxygenase, Oxidoreductase |
Biological process | Purine metabolism |
Ligand | FAD, Flavoprotein, NAD |
Enzyme and pathway databases
BRENDAi | 1.14.13.113, 2814 |
UniPathwayi | UPA00394 |
Names & Taxonomyi
Protein namesi | Recommended name: FAD-dependent urate hydroxylase1 Publication (EC:1.14.13.1131 Publication)Alternative name(s): FAD-dependent urate oxidase1 Publication |
Gene namesi | Name:hpxO1 Publication Ordered Locus Names:KPN78578_16330 ORF Names:KPN_01663 |
Organismi | Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578) |
Taxonomic identifieri | 272620 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Klebsiella › |
Proteomesi |
|
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 204 | R → Q: 160-fold decrease in catalytic activity. Results in the uncoupling of the NADH oxidation and urate hydroxylation reactions. 1 Publication | 1 | |
Mutagenesisi | 216 | Y → F: 5-fold decrease in catalytic activity. 2-fold decrease in affinity for urate and increase in affinity for NADH. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000418845 | 1 – 384 | FAD-dependent urate hydroxylaseAdd BLAST | 384 |
Interactioni
Subunit structurei
Monomer.
1 PublicationProtein-protein interaction databases
STRINGi | 272620.KPN_01663 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | A6T923 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 216 – 218 | Substrate bindingCombined sources1 Publication | 3 |
Sequence similaritiesi
Belongs to the FAD-dependent urate hydroxylase family.Curated
Phylogenomic databases
HOGENOMi | CLU_009665_19_5_6 |
OMAi | RHVMTYT |
Family and domain databases
Gene3Di | 3.50.50.60, 1 hit |
InterProi | View protein in InterPro IPR002938, FAD-bd IPR036188, FAD/NAD-bd_sf |
Pfami | View protein in Pfam PF01494, FAD_binding_3, 1 hit |
SUPFAMi | SSF51905, SSF51905, 1 hit |
i Sequence
Sequence statusi: Complete.
A6T923-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKAIVIGAGI GGLSAAVALK QSGIDCDVYE AVKEIKPVGA AISVWPNGVK
60 70 80 90 100
CMAHLGMGDI METFGGPLRR MAYRDFRSGE NMTQFSLAPL IERTGSRPCP
110 120 130 140 150
VSRAELQREM LDYWGRDSVQ FGKRVTRCEE DADGVTVWFT DGSSASGDLL
160 170 180 190 200
IAADGSHSAL RPWVLGFTPQ RRYAGYVNWN GLVEIDEALA PGDQWTTFVG
210 220 230 240 250
EGKRVSLMPV SAGRFYFFFD VPLPAGLAED RDTLRADLSR YFAGWAPPVQ
260 270 280 290 300
KLIAALDPQT TNRIEIHDIE PFSRLVRGRV ALLGDAGHST TPDIGQGGCA
310 320 330 340 350
AMEDAVVLGA VFRQTRDIAA ALREYEAQRC DRVRDLVLKA RKRCDITHGK
360 370 380
DMQLTEAWYQ ELREETGERI INGMCDTILS GPLG
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000647 Genomic DNA Translation: ABR77094.1 |
RefSeqi | WP_002904783.1, NC_009648.1 |
Genome annotation databases
EnsemblBacteriai | ABR77094; ABR77094; KPN_01663 |
KEGGi | kpn:KPN_01663 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000647 Genomic DNA Translation: ABR77094.1 |
RefSeqi | WP_002904783.1, NC_009648.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3RP6 | X-ray | 2.20 | A | 1-384 | [»] | |
3RP7 | X-ray | 2.04 | A | 1-384 | [»] | |
3RP8 | X-ray | 1.97 | A | 1-384 | [»] | |
SMRi | A6T923 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 272620.KPN_01663 |
Genome annotation databases
EnsemblBacteriai | ABR77094; ABR77094; KPN_01663 |
KEGGi | kpn:KPN_01663 |
Phylogenomic databases
HOGENOMi | CLU_009665_19_5_6 |
OMAi | RHVMTYT |
Enzyme and pathway databases
UniPathwayi | UPA00394 |
BRENDAi | 1.14.13.113, 2814 |
Family and domain databases
Gene3Di | 3.50.50.60, 1 hit |
InterProi | View protein in InterPro IPR002938, FAD-bd IPR036188, FAD/NAD-bd_sf |
Pfami | View protein in Pfam PF01494, FAD_binding_3, 1 hit |
SUPFAMi | SSF51905, SSF51905, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | HPXO_KLEP7 | |
Accessioni | A6T923Primary (citable) accession number: A6T923 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 5, 2012 |
Last sequence update: | August 21, 2007 | |
Last modified: | February 23, 2022 | |
This is version 75 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families