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UniProtKB - A6QP15 (HOT_BOVIN)
Protein
Hydroxyacid-oxoacid transhydrogenase, mitochondrial
Gene
ADHFE1
Organism
Bos taurus (Bovine)
Status
Functioni
Catalyzes the cofactor-independent reversible oxidation of gamma-hydroxybutyrate (GHB) to succinic semialdehyde (SSA) coupled to reduction of 2-ketoglutarate (2-KG) to D-2-hydroxyglutarate (D-2-HG). L-3-hydroxybutyrate (L-3-OHB) is also a substrate for HOT when using 2-KG as hydrogen acceptor, resulting in the formation of D-2-HG (By similarity).
By similarityCatalytic activityi
GO - Molecular functioni
- alcohol dehydrogenase (NAD+) activity Source: GO_Central
- hydroxyacid-oxoacid transhydrogenase activity Source: UniProtKB
- metal ion binding Source: InterPro
GO - Biological processi
- glutamate catabolic process via 2-oxoglutarate Source: UniProtKB
- lipid metabolic process Source: UniProtKB-KW
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Lipid metabolism |
Names & Taxonomyi
Protein namesi | Recommended name: Hydroxyacid-oxoacid transhydrogenase, mitochondrial (EC:1.1.99.24)Short name: HOT Alternative name(s): Alcohol dehydrogenase iron-containing protein 1 Short name: ADHFe1 |
Gene namesi | Name:ADHFE1 |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Organism-specific databases
VEuPathDBi | HostDB:ENSBTAG00000004476 |
VGNCi | VGNC:25674, ADHFE1 |
Subcellular locationi
Mitochondrion
- Mitochondrion By similarity
Mitochondrion
- mitochondrion Source: UniProtKB
Keywords - Cellular componenti
MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – ? | MitochondrionSequence analysis | ||
ChainiPRO_0000322995 | ? – 466 | Hydroxyacid-oxoacid transhydrogenase, mitochondrial |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 444 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 451 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
PaxDbi | A6QP15 |
PeptideAtlasi | A6QP15 |
Family & Domainsi
Sequence similaritiesi
Belongs to the iron-containing alcohol dehydrogenase family. Hydroxyacid-oxoacid transhydrogenase subfamily.Curated
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG3857, Eukaryota |
GeneTreei | ENSGT00390000003849 |
InParanoidi | A6QP15 |
OMAi | IRFGPGC |
OrthoDBi | 776004at2759 |
Family and domain databases
CDDi | cd08190, HOT, 1 hit |
InterProi | View protein in InterPro IPR001670, ADH_Fe/GldA IPR039697, Alcohol_dehydrogenase_Fe IPR042157, HOT |
PANTHERi | PTHR11496, PTHR11496, 1 hit |
Pfami | View protein in Pfam PF00465, Fe-ADH, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
A6QP15-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAAARSRVVH LLRLLQRAAC QCPSHSHTYS QAPGLSPSGK TTDYAFEMAV
60 70 80 90 100
STIRYGAGVT KEVGMDLQSM GAKNVCLMTD KNLSQLPPVQ TVMDSLVKNG
110 120 130 140 150
INFKVYDHVR VEPTDTSFME AIEFAKKGAF DAFLAVGGGS TIDTCKAANL
160 170 180 190 200
YSSSPDSDFL DYVNAPIGKG KPVTVPLKPL IAVPTTSGTG SETTGVAIFD
210 220 230 240 250
YEHLKVKTGI ASRAIKPTLG LIDPLHTLHM PERVVANSGF DVLCHALESY
260 270 280 290 300
TALPYHMRSP CPSSPITRPA YQGSNPISDI WAVHALRIVA KYLKRAIRNP
310 320 330 340 350
DDLEARSNMH LASAFAGIGF GNAGVHLCHG MSYPISGLVK TYKAKDYNVD
360 370 380 390 400
HPLVPHGLSV VLTSPAVFTF TSQMFPERHL EVAEILGADT RTARRPDAGP
410 420 430 440 450
VLADTLRKFL FDLDVDDGLA AIGYSKADIP ELVKGTLPQE RVTKLAPRPQ
460
SEEDLSALFE ASMKLY
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC149097 mRNA Translation: AAI49098.1 |
RefSeqi | NP_001095357.1, NM_001101887.1 |
Genome annotation databases
Ensembli | ENSBTAT00000005874; ENSBTAP00000005874; ENSBTAG00000004476 |
GeneIDi | 507711 |
KEGGi | bta:507711 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC149097 mRNA Translation: AAI49098.1 |
RefSeqi | NP_001095357.1, NM_001101887.1 |
3D structure databases
AlphaFoldDBi | A6QP15 |
SMRi | A6QP15 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9913.ENSBTAP00000005874 |
Proteomic databases
PaxDbi | A6QP15 |
PeptideAtlasi | A6QP15 |
Genome annotation databases
Ensembli | ENSBTAT00000005874; ENSBTAP00000005874; ENSBTAG00000004476 |
GeneIDi | 507711 |
KEGGi | bta:507711 |
Organism-specific databases
CTDi | 137872 |
VEuPathDBi | HostDB:ENSBTAG00000004476 |
VGNCi | VGNC:25674, ADHFE1 |
Phylogenomic databases
eggNOGi | KOG3857, Eukaryota |
GeneTreei | ENSGT00390000003849 |
InParanoidi | A6QP15 |
OMAi | IRFGPGC |
OrthoDBi | 776004at2759 |
Gene expression databases
ExpressionAtlasi | A6QP15, baseline and differential |
Family and domain databases
CDDi | cd08190, HOT, 1 hit |
InterProi | View protein in InterPro IPR001670, ADH_Fe/GldA IPR039697, Alcohol_dehydrogenase_Fe IPR042157, HOT |
PANTHERi | PTHR11496, PTHR11496, 1 hit |
Pfami | View protein in Pfam PF00465, Fe-ADH, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | HOT_BOVIN | |
Accessioni | A6QP15Primary (citable) accession number: A6QP15 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 18, 2008 |
Last sequence update: | August 21, 2007 | |
Last modified: | May 25, 2022 | |
This is version 73 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families