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Protein

Cannabidiolic acid synthase

Gene

CBDAS

Organism
Cannabis sativa (Hemp) (Marijuana)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the stereoselective oxidative cyclization of the monoterpene moiety in cannabigerolic acid (CBGA), producing cannabidiolate (CBDA), the major cannabioid in fiber-type Cannabis plants. Can also use cannabinerolic acid as substrate, but not cannabigerol or cannabinerol.1 Publication

Miscellaneous

CBDA synthase might contribute to the self-defense of Cannabis plants by producing both CBDA and hydrogen peroxide, but since these reaction products are toxic to the plant itself, CBDA synthase is probably secreted from secretory cells into the storage cavity to avoid cellular damage.By similarity

Catalytic activityi

Cannabigerolate + O2 = cannabidiolate + H2O2.2 Publications

Activity regulationi

Inhibited by Hg2+.1 Publication

Kineticsi

  1. KM=0.137 mM for cannabigerolic acid1 Publication
  2. KM=0.206 mM for cannabinerolic acid1 Publication
  1. Vmax=2.57 nmol/sec/mg enzyme with cannabigerolic acid as substrate1 Publication
  2. Vmax=0.39 nmol/sec/mg enzyme with cannabinerolic acid as substrate1 Publication

pH dependencei

Optimum pH is 5.0.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei114FAD (covalent; via 2 links, pros nitrogen)By similarity1
Binding sitei176FAD (covalent; via 2 links)By similarity1
Binding sitei291SubstrateBy similarity1
Binding sitei416SubstrateBy similarity1
Active sitei483Proton acceptorBy similarity1

GO - Molecular functioni

Keywordsi

Molecular functionOxidoreductase
LigandFAD, Flavoprotein

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-12034
BRENDAi1.21.3.8 1159

Names & Taxonomyi

Protein namesi
Recommended name:
Cannabidiolic acid synthase (EC:1.21.3.8)
Alternative name(s):
CBDA synthase
Gene namesi
Name:CBDAS
OrganismiCannabis sativa (Hemp) (Marijuana)
Taxonomic identifieri3483 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsRosalesCannabaceaeCannabis

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Apoplast, Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi114H → A: Loss of FAD binding and loss of catalytic activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 282 PublicationsAdd BLAST28
ChainiPRO_000042114429 – 544Cannabidiolic acid synthaseAdd BLAST516

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi37 ↔ 99By similarity
Glycosylationi45N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi65N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi168N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi296N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi304N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi328N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi498N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

Glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Tissue specificityi

Expressed in young leaves.1 Publication

Interactioni

Subunit structurei

Monomer.1 Publication

Structurei

3D structure databases

ProteinModelPortaliA6P6V9
SMRiA6P6V9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini77 – 251FAD-binding PCMH-typePROSITE-ProRule annotationAdd BLAST175

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi186 – 191Poly-Gly6

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

KOiK20502

Family and domain databases

Gene3Di3.30.43.10, 1 hit
InterProiView protein in InterPro
IPR012951 BBE
IPR016166 FAD-bd_PCMH
IPR036318 FAD-bd_PCMH-like_sf
IPR016167 FAD-bd_PCMH_sub1
IPR006094 Oxid_FAD_bind_N
PfamiView protein in Pfam
PF08031 BBE, 1 hit
PF01565 FAD_binding_4, 1 hit
SUPFAMiSSF56176 SSF56176, 1 hit
PROSITEiView protein in PROSITE
PS51387 FAD_PCMH, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A6P6V9-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKCSTFSFWF VCKIIFFFFS FNIQTSIANP RENFLKCFSQ YIPNNATNLK
60 70 80 90 100
LVYTQNNPLY MSVLNSTIHN LRFTSDTTPK PLVIVTPSHV SHIQGTILCS
110 120 130 140 150
KKVGLQIRTR SGGHDSEGMS YISQVPFVIV DLRNMRSIKI DVHSQTAWVE
160 170 180 190 200
AGATLGEVYY WVNEKNENLS LAAGYCPTVC AGGHFGGGGY GPLMRNYGLA
210 220 230 240 250
ADNIIDAHLV NVHGKVLDRK SMGEDLFWAL RGGGAESFGI IVAWKIRLVA
260 270 280 290 300
VPKSTMFSVK KIMEIHELVK LVNKWQNIAY KYDKDLLLMT HFITRNITDN
310 320 330 340 350
QGKNKTAIHT YFSSVFLGGV DSLVDLMNKS FPELGIKKTD CRQLSWIDTI
360 370 380 390 400
IFYSGVVNYD TDNFNKEILL DRSAGQNGAF KIKLDYVKKP IPESVFVQIL
410 420 430 440 450
EKLYEEDIGA GMYALYPYGG IMDEISESAI PFPHRAGILY ELWYICSWEK
460 470 480 490 500
QEDNEKHLNW IRNIYNFMTP YVSKNPRLAY LNYRDLDIGI NDPKNPNNYT
510 520 530 540
QARIWGEKYF GKNFDRLVKV KTLVDPNNFF RNEQSIPPLP RHRH
Length:544
Mass (Da):62,237
Last modified:August 21, 2007 - v1
Checksum:i199378287274F156
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB292682 mRNA Translation: BAF65033.1

Genome annotation databases

KEGGiag:BAF65033

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB292682 mRNA Translation: BAF65033.1

3D structure databases

ProteinModelPortaliA6P6V9
SMRiA6P6V9
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:BAF65033

Phylogenomic databases

KOiK20502

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-12034
BRENDAi1.21.3.8 1159

Family and domain databases

Gene3Di3.30.43.10, 1 hit
InterProiView protein in InterPro
IPR012951 BBE
IPR016166 FAD-bd_PCMH
IPR036318 FAD-bd_PCMH-like_sf
IPR016167 FAD-bd_PCMH_sub1
IPR006094 Oxid_FAD_bind_N
PfamiView protein in Pfam
PF08031 BBE, 1 hit
PF01565 FAD_binding_4, 1 hit
SUPFAMiSSF56176 SSF56176, 1 hit
PROSITEiView protein in PROSITE
PS51387 FAD_PCMH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCBDAS_CANSA
AccessioniPrimary (citable) accession number: A6P6V9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 6, 2013
Last sequence update: August 21, 2007
Last modified: October 10, 2018
This is version 44 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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