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Protein

Enolase 4

Gene

ENO4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

May be required for sperm motility and function.By similarity

Caution

Although it belongs to the enolase family, Leu-362 is present instead of the conserved Glu which is expected to be an active site residue.Curated

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.By similarity

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.By similarity
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase, Glyceraldehyde-3-phosphate dehydrogenase (HEL-S-162eP), Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), Glyceraldehyde-3-phosphate dehydrogenase (HEL-S-278), Glyceraldehyde-3-phosphate dehydrogenase, testis-specific (GAPDHS), Glyceraldehyde-3-phosphate dehydrogenase (epd)
  2. Phosphoglycerate kinase 2 (PGK2), Phosphoglycerate kinase, Phosphoglycerate kinase, Phosphoglycerate kinase (HEL-S-68p), Phosphoglycerate kinase (HEL-S-272), Phosphoglycerate kinase, Phosphoglycerate kinase, Phosphoglycerate kinase (pgk), Phosphoglycerate kinase 1 (PGK1), Phosphoglycerate kinase
  3. no protein annotated in this organism
  4. Alpha-enolase (ENO1), Beta-enolase (ENO3), Enolase 4 (ENO4), Gamma-enolase (ENO2)
  5. Pyruvate kinase, Pyruvate kinase PKM (PKM), Pyruvate kinase (PKM2), Pyruvate kinase (PKM), Pyruvate kinase (PKM2), Pyruvate kinase (pyk), Pyruvate kinase (PKM), Pyruvate kinase, Pyruvate kinase (PKM), Pyruvate kinase, Pyruvate kinase PKLR (PKLR), Pyruvate kinase, Pyruvate kinase (PKM2), Pyruvate kinase (HEL-S-30), Pyruvate kinase (PKM), Pyruvate kinase (PKM2)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei300SubstrateBy similarity1
Active sitei497Proton acceptorBy similarity1
Binding sitei548SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processGlycolysis

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187

Names & Taxonomyi

Protein namesi
Recommended name:
Enolase 4 (EC:4.2.1.11)
Alternative name(s):
2-phospho-D-glycerate hydro-lyase
Gene namesi
Name:ENO4
Synonyms:C10orf134
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

EuPathDBiHostDB:ENSG00000188316.13
HGNCiHGNC:31670 ENO4
neXtProtiNX_A6NNW6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Organism-specific databases

DisGeNETi387712
OpenTargetsiENSG00000188316

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003484541 – 628Enolase 4Add BLAST628

Proteomic databases

EPDiA6NNW6
PaxDbiA6NNW6
PRIDEiA6NNW6
ProteomicsDBi1643
1644 [A6NNW6-2]

PTM databases

iPTMnetiA6NNW6
PhosphoSitePlusiA6NNW6

Expressioni

Gene expression databases

BgeeiENSG00000188316
ExpressionAtlasiA6NNW6 baseline and differential

Organism-specific databases

HPAiHPA037938
HPA061509

Interactioni

Subunit structurei

Interacts with ENO1 and AKAP4.By similarity

Protein-protein interaction databases

STRINGi9606.ENSP00000345555

Structurei

3D structure databases

ProteinModelPortaliA6NNW6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi189 – 231Pro-richAdd BLAST43

Sequence similaritiesi

Belongs to the enolase family.Curated

Phylogenomic databases

eggNOGiKOG2670 Eukaryota
COG0148 LUCA
GeneTreeiENSGT00910000144064
HOGENOMiHOG000168691
HOVERGENiHBG107850
InParanoidiA6NNW6
PhylomeDBiA6NNW6
TreeFamiTF354238

Family and domain databases

Gene3Di3.20.20.120, 1 hit
3.30.390.10, 1 hit
InterProiView protein in InterPro
IPR000941 Enolase
IPR036849 Enolase-like_C_sf
IPR029017 Enolase-like_N
IPR020810 Enolase_C
IPR020811 Enolase_N
PANTHERiPTHR11902 PTHR11902, 1 hit
PfamiView protein in Pfam
PF00113 Enolase_C, 1 hit
PF03952 Enolase_N, 1 hit
SMARTiView protein in SMART
SM01192 Enolase_C, 1 hit
SM01193 Enolase_N, 1 hit
SUPFAMiSSF51604 SSF51604, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: A6NNW6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEEEGGGRSC GTTRELQKLK QQAMEYYREN DVPRRLEELL NSTFYLQPAD
60 70 80 90 100
VYGHLKANCF SKLAKPPTIC KIVGKDVLDG LGLPTLQVDI FCTIQNFPKN
110 120 130 140 150
VCSVVISTHF EVHENALPEL AKAEEAERAS AVSTAVQWVN STITHELQGM
160 170 180 190 200
APSDQAEVDH LLRIFFASKV QEDKGRKELE KSLEYSTVPT PLPPVPPPPP
210 220 230 240 250
PPPPTKKKGQ KPGRKDTITE KPIAPAEPVE PVLSGSMAIG AVSLAVAKAC
260 270 280 290 300
AMLLNKPLYL NIALLKHNQE QPTTLSMPLL MVSLVSCGKS SSGKLNLMKE
310 320 330 340 350
VICIPHPELT TKQGVEMLME MQKHINKIIE MMPPSPPKAE TKKGHDGSKR
360 370 380 390 400
GQQQITGKMS HLGCLTINCD SIEQPLLLIQ EICANLGLEL GTNLHLAINC
410 420 430 440 450
AGHELMDYNK GKYEVIMGTY KNAAEMVDLY VDLINKYPSI IALIDPFRKE
460 470 480 490 500
DSEQWDSIYH ALGSRCYIIA GTASKSISKL LEQGNISIPK SNGLIIKHTN
510 520 530 540 550
QTTMSDLVEI TNLIDSKKHI TVFGSTEGES SDDSLVDLAV GLGVRFIKLG
560 570 580 590 600
GLSRGERVTK YNRLLTIEEE LVQNGTLGFK EEHTFFYFNE EAEKAAEALE
610 620
AAAAREPLVP TFPTQGVEES AETGASSG
Length:628
Mass (Da):68,821
Last modified:January 15, 2008 - v2
Checksum:iB18C91E2B81A19DA
GO
Isoform 2 (identifier: A6NNW6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     56-333: Missing.
     409-450: Missing.

Show »
Length:308
Mass (Da):33,793
Checksum:i454F89A2A13D18AC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti32V → I in BX647301 (PubMed:17974005).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_03826356 – 333Missing in isoform 2. 1 PublicationAdd BLAST278
Alternative sequenceiVSP_038264409 – 450Missing in isoform 2. 1 PublicationAdd BLAST42

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX647301 mRNA No translation available.
AC023283 Genomic DNA No translation available.
UniGeneiHs.693248

Genome annotation databases

EnsembliENST00000409522; ENSP00000387194; ENSG00000188316 [A6NNW6-2]
UCSCiuc001lcw.4 human [A6NNW6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiENO4_HUMAN
AccessioniPrimary (citable) accession number: A6NNW6
Secondary accession number(s): B8ZZN9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: January 15, 2008
Last modified: June 20, 2018
This is version 82 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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