UniProtKB - A6NNW6 (ENO4_HUMAN)
Protein
Enolase 4
Gene
ENO4
Organism
Homo sapiens (Human)
Status
Functioni
May be required for sperm motility and function.By similarity
Caution
Although it belongs to the enolase family, Leu-362 is present instead of the conserved Glu which is expected to be an active site residue.Curated
Catalytic activityi
- EC:4.2.1.11By similarity
: glycolysis Pathwayi
This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.By similarityProteins known to be involved in the 5 steps of the subpathway in this organism are:
- Glyceraldehyde-3-phosphate dehydrogenase (HEL-S-278), Glyceraldehyde-3-phosphate dehydrogenase, Glyceraldehyde-3-phosphate dehydrogenase (HEL-S-162eP), Glyceraldehyde-3-phosphate dehydrogenase, testis-specific (GAPDHS), Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
- Phosphoglycerate kinase, Phosphoglycerate kinase 2 (PGK2), Phosphoglycerate kinase, Phosphoglycerate kinase, Phosphoglycerate kinase 1 (PGK1), Phosphoglycerate kinase (HEL-S-68p), Phosphoglycerate kinase (HEL-S-272), Phosphoglycerate kinase, Phosphoglycerate kinase, Phosphoglycerate kinase
- no protein annotated in this organism
- Beta-enolase (ENO3), Alpha-enolase (ENO1), 2-phospho-D-glycerate hydro-lyase, 2-phospho-D-glycerate hydro-lyase (ENO4), 2-phospho-D-glycerate hydro-lyase, 2-phospho-D-glycerate hydro-lyase (HEL-S-17), 2-phospho-D-glycerate hydro-lyase (ENO4), 2-phospho-D-glycerate hydro-lyase (ENO1), 2-phospho-D-glycerate hydro-lyase (ENO3), 2-phospho-D-glycerate hydro-lyase (ENO2), 2-phospho-D-glycerate hydro-lyase (ENO1), 2-phospho-D-glycerate hydro-lyase (ENO3), 2-phospho-D-glycerate hydro-lyase (ENO2), Enolase 4 (ENO4), 2-phospho-D-glycerate hydro-lyase (ENO1), 2-phospho-D-glycerate hydro-lyase (ENO3), 2-phospho-D-glycerate hydro-lyase (ENO1), 2-phospho-D-glycerate hydro-lyase, 2-phospho-D-glycerate hydro-lyase, 2-phospho-D-glycerate hydro-lyase, 2-phospho-D-glycerate hydro-lyase (ENO3), 2-phospho-D-glycerate hydro-lyase (ENO2), 2-phospho-D-glycerate hydro-lyase (ENO2), 2-phospho-D-glycerate hydro-lyase (EDARADD), Gamma-enolase (ENO2)
- Pyruvate kinase, Pyruvate kinase PKLR (PKLR), Pyruvate kinase, Pyruvate kinase, Pyruvate kinase PKM (PKM), Pyruvate kinase (PKM2), Pyruvate kinase (PKLR), Pyruvate kinase (PKM), Pyruvate kinase (PKM2), Pyruvate kinase (PKM), Pyruvate kinase (HEL-S-30), Pyruvate kinase (PKM), Pyruvate kinase (PKM), Pyruvate kinase (PKM), Pyruvate kinase (PKM), Pyruvate kinase (PKM2), Pyruvate kinase (PKM), Pyruvate kinase (PKLR), Pyruvate kinase (PKLR), Pyruvate kinase (PKM2), Pyruvate kinase, Pyruvate kinase (PKM)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 300 | SubstrateBy similarity | 1 | |
Active sitei | 497 | Proton acceptorBy similarity | 1 | |
Binding sitei | 548 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- magnesium ion binding Source: InterPro
- phosphopyruvate hydratase activity Source: GO_Central
GO - Biological processi
- glycolytic process Source: GO_Central
- regulation of vacuole fusion, non-autophagic Source: GO_Central
Keywordsi
Molecular function | Lyase |
Biological process | Glycolysis |
Enzyme and pathway databases
PathwayCommonsi | A6NNW6 |
UniPathwayi | UPA00109;UER00187 |
Names & Taxonomyi
Protein namesi | Recommended name: Enolase 4Curated (EC:4.2.1.11By similarity)Alternative name(s): 2-phospho-D-glycerate hydro-lyase |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000188316.13 |
HGNCi | HGNC:31670, ENO4 |
neXtProti | NX_A6NNW6 |
Subcellular locationi
Cytosol
- phosphopyruvate hydratase complex Source: GO_Central
Pathology & Biotechi
Organism-specific databases
DisGeNETi | 387712 |
OpenTargetsi | ENSG00000188316 |
Miscellaneous databases
Pharosi | A6NNW6, Tdark |
Polymorphism and mutation databases
BioMutai | ENO4 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000348454 | 1 – 625 | Enolase 4Add BLAST | 625 |
Post-translational modificationi
Synthesized as an approximately 70-kDa precursor, which then undergoes proteolytic cleavage to an approximately 60-kDa enzyme; HOATZ associates directly or indirectly with ENO4 to mediate this process before its transport to mature flagella.By similarity
Proteomic databases
EPDi | A6NNW6 |
MassIVEi | A6NNW6 |
PaxDbi | A6NNW6 |
PeptideAtlasi | A6NNW6 |
PRIDEi | A6NNW6 |
ProteomicsDBi | 1254 1644 [A6NNW6-2] |
PTM databases
iPTMneti | A6NNW6 |
PhosphoSitePlusi | A6NNW6 |
Expressioni
Gene expression databases
Bgeei | ENSG00000188316, Expressed in nucleus accumbens and 106 other tissues |
Organism-specific databases
HPAi | ENSG00000188316, Tissue enhanced (brain, epididymis, fallopian tube) |
Interactioni
Subunit structurei
Interacts with ENO1 and AKAP4.
By similarityProtein-protein interaction databases
IntActi | A6NNW6, 2 interactors |
STRINGi | 9606.ENSP00000345555 |
Miscellaneous databases
RNActi | A6NNW6, protein |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 189 – 231 | Pro-richAdd BLAST | 43 |
Sequence similaritiesi
Belongs to the enolase family.Curated
Phylogenomic databases
eggNOGi | KOG2670, Eukaryota |
GeneTreei | ENSGT00950000182805 |
HOGENOMi | CLU_1079780_0_0_1 |
InParanoidi | A6NNW6 |
OrthoDBi | 1097401at2759 |
PhylomeDBi | A6NNW6 |
TreeFami | TF354238 |
Family and domain databases
Gene3Di | 3.20.20.120, 1 hit 3.30.390.10, 1 hit |
InterProi | View protein in InterPro IPR000941, Enolase IPR036849, Enolase-like_C_sf IPR029017, Enolase-like_N IPR020810, Enolase_C IPR020811, Enolase_N |
PANTHERi | PTHR11902, PTHR11902, 1 hit |
Pfami | View protein in Pfam PF00113, Enolase_C, 1 hit |
SMARTi | View protein in SMART SM01192, Enolase_C, 1 hit SM01193, Enolase_N, 1 hit |
SUPFAMi | SSF51604, SSF51604, 1 hit SSF54826, SSF54826, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform 3 (identifier: A6NNW6-3) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MEEEGGGRSC GTTRELQKLK QQAMEYYREN DVPRRLEELL NSTFYLQPAD
60 70 80 90 100
VYGHLANCFS KLAKPPTICK IVGKDVLDGL GLPTLQVDIF CTIQNFPKNV
110 120 130 140 150
CSVVISTHFE VHENALPELA KAEEAERASA VSTAVQWVNS TITHELQGMA
160 170 180 190 200
PSDQAEVDHL LRIFFASKVQ EDKGRKELEK SLEYSTVPTP LPPVPPPPPP
210 220 230 240 250
PPPTKKKGQK PGRKDTITEK PIAPAEPVEP VLSGSMAIGA VSLAVAKACA
260 270 280 290 300
MLLNKPLYLN IALLKHNQEQ PTTLSMPLLM VSLVSCGKSS SGKLNLMKEV
310 320 330 340 350
ICIPHPELTT KQGVEMLMEM QKHINKIIEM PSPPKAETKK GHDGSKRGQQ
360 370 380 390 400
QITGKMSHLG CLTINCDSIE QPLLLIQEIC ANLGLELGTN LHLAINCAGH
410 420 430 440 450
ELMDYNKGKY EVIMGTYKNA AEMVDLYVDL INKYPSIIAL IDPFRKEDSE
460 470 480 490 500
QWDSIYHALG SRCYIIAGTA SKSISKLLEQ GNISIPKSNG LIIKHTNQTT
510 520 530 540 550
MSDLVEITNL IDSKKHITVF GSTEGESSDD SLVDLAVGLG VRFIKLGGLS
560 570 580 590 600
RGERVTKYNR LLTIEEELVQ NGTLGFKEEH TFFYFNEEAE KAAEALEAAA
610 620
AREPLVPTFP TQGVEESAET GASSG
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A5H1ZRS3 | A0A5H1ZRS3_HUMAN | 2-phospho-D-glycerate hydro-lyase | ENO4 | 628 | Annotation score: | ||
A0A5H1ZRQ0 | A0A5H1ZRQ0_HUMAN | 2-phospho-D-glycerate hydro-lyase | ENO4 | 422 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 32 | V → I in BX647301 (PubMed:17974005).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_059949 | 1 – 235 | Missing in isoform 4. Add BLAST | 235 | |
Alternative sequenceiVSP_059947 | 56 – 330 | Missing in isoform 2. Add BLAST | 275 | |
Alternative sequenceiVSP_059948 | 406 – 448 | NKGKY…FRKED → D in isoform 2. Add BLAST | 43 | |
Alternative sequenceiVSP_059951 | 575 – 598 | GFKEE…EALEA → ATSSSSCDARIGGQEQETMT LVKV in isoform 4. Add BLAST | 24 | |
Alternative sequenceiVSP_059950 | 599 – 625 | Missing in isoform 4. Add BLAST | 27 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BX647301 mRNA No translation available. AC023283 Genomic DNA No translation available. |
CCDSi | CCDS73206.1 [A6NNW6-3] |
RefSeqi | NP_001229628.1, NM_001242699.1 [A6NNW6-3] |
Genome annotation databases
Ensembli | ENST00000341276; ENSP00000345555; ENSG00000188316 [A6NNW6-3] ENST00000409522; ENSP00000387194; ENSG00000188316 [A6NNW6-2] |
GeneIDi | 387712 |
KEGGi | hsa:387712 |
UCSCi | uc001lcw.4, human [A6NNW6-3] uc057wfb.1, human uc057wfd.1, human |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BX647301 mRNA No translation available. AC023283 Genomic DNA No translation available. |
CCDSi | CCDS73206.1 [A6NNW6-3] |
RefSeqi | NP_001229628.1, NM_001242699.1 [A6NNW6-3] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
IntActi | A6NNW6, 2 interactors |
STRINGi | 9606.ENSP00000345555 |
PTM databases
iPTMneti | A6NNW6 |
PhosphoSitePlusi | A6NNW6 |
Polymorphism and mutation databases
BioMutai | ENO4 |
Proteomic databases
EPDi | A6NNW6 |
MassIVEi | A6NNW6 |
PaxDbi | A6NNW6 |
PeptideAtlasi | A6NNW6 |
PRIDEi | A6NNW6 |
ProteomicsDBi | 1254 1644 [A6NNW6-2] |
Protocols and materials databases
Antibodypediai | 49029, 79 antibodies |
Genome annotation databases
Ensembli | ENST00000341276; ENSP00000345555; ENSG00000188316 [A6NNW6-3] ENST00000409522; ENSP00000387194; ENSG00000188316 [A6NNW6-2] |
GeneIDi | 387712 |
KEGGi | hsa:387712 |
UCSCi | uc001lcw.4, human [A6NNW6-3] uc057wfb.1, human uc057wfd.1, human |
Organism-specific databases
CTDi | 387712 |
DisGeNETi | 387712 |
EuPathDBi | HostDB:ENSG00000188316.13 |
GeneCardsi | ENO4 |
HGNCi | HGNC:31670, ENO4 |
HPAi | ENSG00000188316, Tissue enhanced (brain, epididymis, fallopian tube) |
neXtProti | NX_A6NNW6 |
OpenTargetsi | ENSG00000188316 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2670, Eukaryota |
GeneTreei | ENSGT00950000182805 |
HOGENOMi | CLU_1079780_0_0_1 |
InParanoidi | A6NNW6 |
OrthoDBi | 1097401at2759 |
PhylomeDBi | A6NNW6 |
TreeFami | TF354238 |
Enzyme and pathway databases
UniPathwayi | UPA00109;UER00187 |
PathwayCommonsi | A6NNW6 |
Miscellaneous databases
BioGRID-ORCSi | 387712, 2 hits in 110 CRISPR screens |
ChiTaRSi | ENO4, human |
GenomeRNAii | 387712 |
Pharosi | A6NNW6, Tdark |
PROi | PR:A6NNW6 |
RNActi | A6NNW6, protein |
Gene expression databases
Bgeei | ENSG00000188316, Expressed in nucleus accumbens and 106 other tissues |
Family and domain databases
Gene3Di | 3.20.20.120, 1 hit 3.30.390.10, 1 hit |
InterProi | View protein in InterPro IPR000941, Enolase IPR036849, Enolase-like_C_sf IPR029017, Enolase-like_N IPR020810, Enolase_C IPR020811, Enolase_N |
PANTHERi | PTHR11902, PTHR11902, 1 hit |
Pfami | View protein in Pfam PF00113, Enolase_C, 1 hit |
SMARTi | View protein in SMART SM01192, Enolase_C, 1 hit SM01193, Enolase_N, 1 hit |
SUPFAMi | SSF51604, SSF51604, 1 hit SSF54826, SSF54826, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ENO4_HUMAN | |
Accessioni | A6NNW6Primary (citable) accession number: A6NNW6 Secondary accession number(s): A0A087WZY6 J3KNX1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 2, 2008 |
Last sequence update: | December 5, 2018 | |
Last modified: | December 2, 2020 | |
This is version 101 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families - Human chromosome 10
Human chromosome 10: entries, gene names and cross-references to MIM