UniProtKB - A6NJ78 (MET15_HUMAN)
Protein
12S rRNA N4-methylcytidine (m4C) methyltransferase
Gene
METTL15
Organism
Homo sapiens (Human)
Status
Functioni
N4-methylcytidine (m4C) methyltransferase responsible for the methylation of position C839 in mitochondrial 12S rRNA (PubMed:31665743). Involved in the stabilization of 12S rRNA folding, therefore facilitating the assembly of the mitochondrial small ribosomal subunits (PubMed:31665743).1 Publication
Catalytic activityi
- cytidine839 in 12S rRNA + S-adenosyl-L-methionine = H+ + N4-methylcytidine839 in 12S rRNA + S-adenosyl-L-homocysteine1 PublicationThis reaction proceeds in the forward1 Publication direction.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 119 | S-adenosyl-L-methionineBy similarity | 1 | |
Binding sitei | 146 | S-adenosyl-L-methionine; via amide nitrogenBy similarity | 1 | |
Binding sitei | 169 | S-adenosyl-L-methionineBy similarity | 1 | |
Binding sitei | 176 | S-adenosyl-L-methionineBy similarity | 1 |
GO - Molecular functioni
- rRNA (cytosine-N4-)-methyltransferase activity Source: GO_Central
GO - Biological processi
- rRNA base methylation Source: GO_Central
Keywordsi
Molecular function | Methyltransferase, Transferase |
Ligand | S-adenosyl-L-methionine |
Enzyme and pathway databases
PathwayCommonsi | A6NJ78 |
Names & Taxonomyi
Protein namesi | Recommended name: 12S rRNA N4-methylcytidine (m4C) methyltransferase (EC:2.1.1.-1 Publication)Short name: 12S rRNA m4C methyltransferase Alternative name(s): Methyltransferase 5 domain-containing protein 1 Methyltransferase-like protein 15 |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000169519.20 |
HGNCi | HGNC:26606, METTL15 |
MIMi | 618711, gene |
neXtProti | NX_A6NJ78 |
Subcellular locationi
Mitochondrion
- Mitochondrion matrix 1 Publication
Mitochondrion
- mitochondrial matrix Source: UniProtKB-SubCell
Keywords - Cellular componenti
MitochondrionPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 119 – 120 | DR → AA: Absence of methylation of 12S rRNA position C839 and decreased methylation at position C841. 1 Publication | 2 | |
Mutagenesisi | 298 | E → A: Decreased methylation of 12S rRNA positions C839 and C841. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 196074 |
OpenTargetsi | ENSG00000169519 |
PharmGKBi | PA142671458 |
Miscellaneous databases
Pharosi | A6NJ78, Tdark |
Polymorphism and mutation databases
BioMutai | METTL15 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – ? | MitochondrionSequence analysis | ||
ChainiPRO_0000308332 | ? – 407 | 12S rRNA N4-methylcytidine (m4C) methyltransferase |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 358 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | A6NJ78 |
jPOSTi | A6NJ78 |
MassIVEi | A6NJ78 |
MaxQBi | A6NJ78 |
PaxDbi | A6NJ78 |
PeptideAtlasi | A6NJ78 |
PRIDEi | A6NJ78 |
ProteomicsDBi | 1313 [A6NJ78-1] 1314 [A6NJ78-2] 1315 [A6NJ78-3] 1316 [A6NJ78-4] |
PTM databases
iPTMneti | A6NJ78 |
PhosphoSitePlusi | A6NJ78 |
Expressioni
Gene expression databases
Bgeei | ENSG00000169519, Expressed in adrenal tissue and 196 other tissues |
ExpressionAtlasi | A6NJ78, baseline and differential |
Genevisiblei | A6NJ78, HS |
Organism-specific databases
HPAi | ENSG00000169519, Low tissue specificity |
Interactioni
Binary interactionsi
Hide detailsIsoform 4 [A6NJ78-4]
Protein-protein interaction databases
BioGRIDi | 128190, 37 interactors |
IntActi | A6NJ78, 32 interactors |
STRINGi | 9606.ENSP00000384369 |
Miscellaneous databases
RNActi | A6NJ78, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 100 – 102 | S-adenosyl-L-methionine bindingBy similarity | 3 |
Sequence similaritiesi
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG2782, Eukaryota |
GeneTreei | ENSGT00390000014756 |
HOGENOMi | CLU_038422_1_0_1 |
InParanoidi | A6NJ78 |
OMAi | NPAKRTF |
OrthoDBi | 809593at2759 |
PhylomeDBi | A6NJ78 |
TreeFami | TF106425 |
Family and domain databases
Gene3Di | 1.10.150.170, 1 hit |
HAMAPi | MF_01007, 16SrRNA_methyltr_H, 1 hit |
InterProi | View protein in InterPro IPR002903, RsmH IPR023397, SAM-dep_MeTrfase_MraW_recog IPR029063, SAM-dependent_MTases |
PANTHERi | PTHR11265, PTHR11265, 1 hit |
Pfami | View protein in Pfam PF01795, Methyltransf_5, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit SSF81799, SSF81799, 1 hit |
TIGRFAMsi | TIGR00006, TIGR00006, 1 hit |
s (4+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: A6NJ78-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MLRYPYFCRM YKECLSCWLE SGIPNLGVWP NRIHTTAEKY REYEAREQTD
60 70 80 90 100
QTQAQELHRS QDRDFETMAK LHIPVMVDEV VHCLSPQKGQ IFLDMTFGSG
110 120 130 140 150
GHTKAILQKE SDIVLYALDR DPTAYALAEH LSELYPKQIR AMLGQFSQAE
160 170 180 190 200
ALLMKAGVQP GTFDGVLMDL GCSSMQLDTP ERGFSLRKDG PLDMRMDGGR
210 220 230 240 250
YPDMPTAADV VNALDQQALA SILRTYGEEK HAKKIASAIV QARSIYPITR
260 270 280 290 300
TQQLASIVAG AFPPSAIYTR KDLLQRSTHI ATKTFQALRI FVNNELNELY
310 320 330 340 350
TGLKTAQKFL RPGGRLVALS FHSLEDRIVK RFLLGISMTE RFNLSVRQQV
360 370 380 390 400
MKTSQLGSDH ENTEEVSMRR APLMWELIHK KVLSPQDQDV QDNPRGRSAK
LRAAIKL
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF8WD83 | F8WD83_HUMAN | 12S rRNA N4-methylcytidine (m4C) me... | METTL15 | 164 | Annotation score: | ||
A0A0U1RR76 | A0A0U1RR76_HUMAN | 12S rRNA N4-methylcytidine (m4C) me... | METTL15 | 274 | Annotation score: | ||
B5MC64 | B5MC64_HUMAN | 12S rRNA N4-methylcytidine (m4C) me... | METTL15 | 93 | Annotation score: | ||
A0A0U1RR64 | A0A0U1RR64_HUMAN | 12S rRNA N4-methylcytidine (m4C) me... | METTL15 | 107 | Annotation score: | ||
A0A0U1RRD2 | A0A0U1RRD2_HUMAN | 12S rRNA N4-methylcytidine (m4C) me... | METTL15 | 149 | Annotation score: | ||
A0A0U1RRF2 | A0A0U1RRF2_HUMAN | 12S rRNA N4-methylcytidine (m4C) me... | METTL15 | 90 | Annotation score: |
Sequence cautioni
The sequence AAH30997 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 191 | P → S in AAH30997 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 194 | M → V in BAC03631 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_036801 | 31 | N → K2 PublicationsCorresponds to variant dbSNP:rs2883478Ensembl. | 1 | |
Natural variantiVAR_036802 | 149 | A → T. Corresponds to variant dbSNP:rs11823114Ensembl. | 1 | |
Natural variantiVAR_059446 | 267 | I → F. Corresponds to variant dbSNP:rs11030280Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_028970 | 91 – 99 | IFLDMTFGS → SLGVRSVGI in isoform 3. 1 Publication | 9 | |
Alternative sequenceiVSP_028971 | 100 – 407 | Missing in isoform 3. 1 PublicationAdd BLAST | 308 | |
Alternative sequenceiVSP_028972 | 201 – 320 | YPDMP…LVALS → STGTCIYPKNIRGGEACQEN RFSNCSGTQHLPHHQNPAAC QHRCRSISSLCYLYTERLTT AIYPYCHQDFPGSSHICEQ in isoform 4. 1 PublicationAdd BLAST | 120 | |
Alternative sequenceiVSP_028973 | 260 – 273 | GAFPP…TRKDL → EYSETYFPVAQTLY in isoform 2. 1 PublicationAdd BLAST | 14 | |
Alternative sequenceiVSP_028974 | 274 – 407 | Missing in isoform 2. 1 PublicationAdd BLAST | 134 | |
Alternative sequenceiVSP_028975 | 321 – 407 | Missing in isoform 4. 1 PublicationAdd BLAST | 87 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK091298 mRNA Translation: BAC03631.1 AC023206 Genomic DNA No translation available. AC087376 Genomic DNA No translation available. CH471064 Genomic DNA Translation: EAW68267.1 CH471064 Genomic DNA Translation: EAW68266.1 BC030997 mRNA Translation: AAH30997.1 Different initiation. BC105287 mRNA Translation: AAI05288.1 |
CCDSi | CCDS31450.1 [A6NJ78-2] CCDS44559.1 [A6NJ78-1] CCDS73269.1 [A6NJ78-4] |
RefSeqi | NP_001107000.1, NM_001113528.1 [A6NJ78-1] NP_001284704.1, NM_001297775.1 [A6NJ78-4] NP_689849.2, NM_152636.2 [A6NJ78-2] XP_016872786.1, XM_017017297.1 [A6NJ78-1] |
Genome annotation databases
Ensembli | ENST00000303459; ENSP00000307251; ENSG00000169519 [A6NJ78-2] ENST00000406787; ENSP00000385507; ENSG00000169519 [A6NJ78-4] ENST00000407364; ENSP00000384369; ENSG00000169519 [A6NJ78-1] ENST00000437814; ENSP00000392806; ENSG00000169519 [A6NJ78-3] |
GeneIDi | 196074 |
KEGGi | hsa:196074 |
UCSCi | uc001mse.3, human [A6NJ78-1] |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK091298 mRNA Translation: BAC03631.1 AC023206 Genomic DNA No translation available. AC087376 Genomic DNA No translation available. CH471064 Genomic DNA Translation: EAW68267.1 CH471064 Genomic DNA Translation: EAW68266.1 BC030997 mRNA Translation: AAH30997.1 Different initiation. BC105287 mRNA Translation: AAI05288.1 |
CCDSi | CCDS31450.1 [A6NJ78-2] CCDS44559.1 [A6NJ78-1] CCDS73269.1 [A6NJ78-4] |
RefSeqi | NP_001107000.1, NM_001113528.1 [A6NJ78-1] NP_001284704.1, NM_001297775.1 [A6NJ78-4] NP_689849.2, NM_152636.2 [A6NJ78-2] XP_016872786.1, XM_017017297.1 [A6NJ78-1] |
3D structure databases
SMRi | A6NJ78 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 128190, 37 interactors |
IntActi | A6NJ78, 32 interactors |
STRINGi | 9606.ENSP00000384369 |
PTM databases
iPTMneti | A6NJ78 |
PhosphoSitePlusi | A6NJ78 |
Polymorphism and mutation databases
BioMutai | METTL15 |
Proteomic databases
EPDi | A6NJ78 |
jPOSTi | A6NJ78 |
MassIVEi | A6NJ78 |
MaxQBi | A6NJ78 |
PaxDbi | A6NJ78 |
PeptideAtlasi | A6NJ78 |
PRIDEi | A6NJ78 |
ProteomicsDBi | 1313 [A6NJ78-1] 1314 [A6NJ78-2] 1315 [A6NJ78-3] 1316 [A6NJ78-4] |
Protocols and materials databases
Antibodypediai | 25483, 44 antibodies |
DNASUi | 196074 |
Genome annotation databases
Ensembli | ENST00000303459; ENSP00000307251; ENSG00000169519 [A6NJ78-2] ENST00000406787; ENSP00000385507; ENSG00000169519 [A6NJ78-4] ENST00000407364; ENSP00000384369; ENSG00000169519 [A6NJ78-1] ENST00000437814; ENSP00000392806; ENSG00000169519 [A6NJ78-3] |
GeneIDi | 196074 |
KEGGi | hsa:196074 |
UCSCi | uc001mse.3, human [A6NJ78-1] |
Organism-specific databases
CTDi | 196074 |
DisGeNETi | 196074 |
EuPathDBi | HostDB:ENSG00000169519.20 |
GeneCardsi | METTL15 |
HGNCi | HGNC:26606, METTL15 |
HPAi | ENSG00000169519, Low tissue specificity |
MIMi | 618711, gene |
neXtProti | NX_A6NJ78 |
OpenTargetsi | ENSG00000169519 |
PharmGKBi | PA142671458 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2782, Eukaryota |
GeneTreei | ENSGT00390000014756 |
HOGENOMi | CLU_038422_1_0_1 |
InParanoidi | A6NJ78 |
OMAi | NPAKRTF |
OrthoDBi | 809593at2759 |
PhylomeDBi | A6NJ78 |
TreeFami | TF106425 |
Enzyme and pathway databases
PathwayCommonsi | A6NJ78 |
Miscellaneous databases
BioGRID-ORCSi | 196074, 70 hits in 821 CRISPR screens |
ChiTaRSi | METTL15, human |
GenomeRNAii | 196074 |
Pharosi | A6NJ78, Tdark |
PROi | PR:A6NJ78 |
RNActi | A6NJ78, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000169519, Expressed in adrenal tissue and 196 other tissues |
ExpressionAtlasi | A6NJ78, baseline and differential |
Genevisiblei | A6NJ78, HS |
Family and domain databases
Gene3Di | 1.10.150.170, 1 hit |
HAMAPi | MF_01007, 16SrRNA_methyltr_H, 1 hit |
InterProi | View protein in InterPro IPR002903, RsmH IPR023397, SAM-dep_MeTrfase_MraW_recog IPR029063, SAM-dependent_MTases |
PANTHERi | PTHR11265, PTHR11265, 1 hit |
Pfami | View protein in Pfam PF01795, Methyltransf_5, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit SSF81799, SSF81799, 1 hit |
TIGRFAMsi | TIGR00006, TIGR00006, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MET15_HUMAN | |
Accessioni | A6NJ78Primary (citable) accession number: A6NJ78 Secondary accession number(s): A8MRS5 Q8NBA7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 23, 2007 |
Last sequence update: | July 24, 2007 | |
Last modified: | December 2, 2020 | |
This is version 114 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Human chromosome 11
Human chromosome 11: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations