METTL15

Functionⁱ

N4-methylcytidine (m4C) methyltransferase responsible for the methylation of position C839 in mitochondrial 12S rRNA (PubMed:31665743).

Involved in the stabilization of 12S rRNA folding, therefore facilitating the assembly of the mitochondrial small ribosomal subunits (PubMed:31665743).

1 Publication

Catalytic activityⁱ

cytidine⁸³⁹ in 12S rRNA
Search proteins in UniProtKB for this molecule.
Search chemical reactions in Rhea for this molecule.
+
S-adenosyl-L-methionine
Search proteins in UniProtKB for this molecule.
Search chemical reactions in Rhea for this molecule.
See the description of this molecule in ChEBI.
=
H⁺
Search proteins in UniProtKB for this molecule.
Search chemical reactions in Rhea for this molecule.
See the description of this molecule in ChEBI.
+
N⁴-methylcytidine⁸³⁹ in 12S rRNA
Search proteins in UniProtKB for this molecule.
Search chemical reactions in Rhea for this molecule.
+
S-adenosyl-L-homocysteine
Search proteins in UniProtKB for this molecule.
Search chemical reactions in Rhea for this molecule.
See the description of this molecule in ChEBI.
1 Publication
Manual assertion based on experiment inⁱ
- Ref.6
  "METTL15 introduces N4-methylcytidine into human mitochondrial 12S rRNA and is required for mitoribosome biogenesis."
  Van Haute L., Hendrick A.G., D'Souza A.R., Powell C.A., Rebelo-Guiomar P., Harbour M.E., Ding S., Fearnley I.M., Andrews B., Minczuk M.
  Nucleic Acids Res. 47:10267-10281(2019) [PubMed] [Europe PMC] [Abstract]
  Cited for: FUNCTION, SUBCELLULAR LOCATION, CATALYTIC ACTIVITY, MUTAGENESIS OF 119-ASP-ARG-120 AND GLU-298.
This reaction proceeds in the
forward
Search for this reaction in UniProtKB.
See the description of this reaction in Rhea.
1 Publication
Manual assertion based on experiment inⁱ
- Ref.6
  "METTL15 introduces N4-methylcytidine into human mitochondrial 12S rRNA and is required for mitoribosome biogenesis."
  Van Haute L., Hendrick A.G., D'Souza A.R., Powell C.A., Rebelo-Guiomar P., Harbour M.E., Ding S., Fearnley I.M., Andrews B., Minczuk M.
  Nucleic Acids Res. 47:10267-10281(2019) [PubMed] [Europe PMC] [Abstract]
  Cited for: FUNCTION, SUBCELLULAR LOCATION, CATALYTIC ACTIVITY, MUTAGENESIS OF 119-ASP-ARG-120 AND GLU-298.
direction.
Source:
Rhea
Search for this reaction in UniProtKB.
See the description of this reaction in Rhea.
. Show »« Hide
cytidine⁸³⁹ in 12S rRNA
CMP residue
zoom
+
S-adenosyl-L-methionine
zoom
=
H⁺
+
N⁴-methylcytidine⁸³⁹ in 12S rRNA
N⁴-methylcytidine 5'-phosphate residue
zoom
+
S-adenosyl-L-homocysteine
zoom

Sites

Feature key	Position(s)	DescriptionActions	Length
Binding siteⁱ	119	S-adenosyl-L-methionineBy similarity	1
Binding siteⁱ	146	S-adenosyl-L-methionine; via amide nitrogenBy similarity	1
Binding siteⁱ	169	S-adenosyl-L-methionineBy similarity	1
Binding siteⁱ	176	S-adenosyl-L-methionineBy similarity	1

GO - Molecular functionⁱ

rRNA (cytosine-N4-)-methyltransferase activity
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- "Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium."
  Gaudet P., Livstone M.S., Lewis S.E., Thomas P.D.
  Brief Bioinform 12:449-462(2011) [PubMed] [Europe PMC] [Abstract]

Complete GO annotation on QuickGO ...

GO - Biological processⁱ

rRNA base methylation
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- "Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium."
  Gaudet P., Livstone M.S., Lewis S.E., Thomas P.D.
  Brief Bioinform 12:449-462(2011) [PubMed] [Europe PMC] [Abstract]

Complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Methyltransferase, Transferase
Ligand	S-adenosyl-L-methionine

Enzyme and pathway databases

PathwayCommonsⁱ	A6NJ78

PathwayCommonsⁱ

A6NJ78

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: 12S rRNA N4-methylcytidine (m4C) methyltransferase (EC:2.1.1.-1 Publication) Short name: 12S rRNA m4C methyltransferase Alternative name(s): Methyltransferase 5 domain-containing protein 1 Methyltransferase-like protein 15
Gene namesⁱ	Name:METTL15Imported Synonyms:METT5D1
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 11

Organism-specific databases

Human Gene Nomenclature Database More...HGNCⁱ	HGNC:26606, METTL15
MIMⁱ	618711, gene
neXtProtⁱ	NX_A6NJ78
VEuPathDBⁱ	HostDB:ENSG00000169519

Keywords - Cellular componentⁱ

Mitochondrion

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Mutagenesisⁱ	119 – 120	DR → AA: Absence of methylation of 12S rRNA position C839 and decreased methylation at position C841. 1 Publication		2
Mutagenesisⁱ	298	E → A: Decreased methylation of 12S rRNA positions C839 and C841. 1 Publication		1

Organism-specific databases

DisGeNET More...DisGeNETⁱ	196074
Open Targets More...OpenTargetsⁱ	ENSG00000169519
PharmGKBⁱ	PA142671458

Miscellaneous databases

Pharosⁱ	A6NJ78, Tdark

Pharosⁱ

A6NJ78, Tdark

Genetic variation databases

BioMutaⁱ	METTL15

BioMutaⁱ

METTL15

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Transit peptideⁱ	1 – ?	MitochondrionSequence analysis
ChainⁱPRO_0000308332	? – 407	12S rRNA N4-methylcytidine (m4C) methyltransferase

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Modified residueⁱ	358	PhosphoserineCombined sources		1

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

EPDⁱ	A6NJ78
jPOSTⁱ	A6NJ78
MassIVEⁱ	A6NJ78
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	A6NJ78
PaxDbⁱ	A6NJ78
PeptideAtlas More...PeptideAtlasⁱ	A6NJ78
PRIDEⁱ	A6NJ78
ProteomicsDBⁱ	1313 [A6NJ78-1] 1314 [A6NJ78-2] 1315 [A6NJ78-3] 1316 [A6NJ78-4]

PTM databases

GlyGenⁱ	A6NJ78, 1 site, 1 O-linked glycan (1 site)
iPTMnetⁱ	A6NJ78
PhosphoSitePlusⁱ	A6NJ78

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSG00000169519, Expressed in adrenal tissue and 197 other tissues
ExpressionAtlasⁱ	A6NJ78, baseline and differential
Genevisibleⁱ	A6NJ78, HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	ENSG00000169519, Low tissue specificity

HPAⁱ

ENSG00000169519, Low tissue specificity

Interactionⁱ

Binary interactionsⁱ

Hide details

Isoform 4 [A6NJ78-4]

With	#Exp.	IntAct
CASP6 [P55212]	3	EBI-10174029,EBI-718729
CIDEC [Q96AQ7]	3	EBI-10174029,EBI-14151404
CSTF2 [P33240]	3	EBI-10174029,EBI-711360
DMWD [G5E9A7]	3	EBI-10174029,EBI-10976677
EIF2S3 [P41091]	3	EBI-10174029,EBI-1054228
FANCL [Q9NW38]	3	EBI-10174029,EBI-2339898
FKBP1A [Q0VDC6]	3	EBI-10174029,EBI-10226858
GLYCTK [Q8IVS8]	3	EBI-10174029,EBI-748515
HOXC8 [P31273]	3	EBI-10174029,EBI-1752118
LAMP2 - isoform LAMP-2B [P13473-2]	3	EBI-10174029,EBI-21591415
MAPKBP1 [O60336]	3	EBI-10174029,EBI-947402
MSRB3 - isoform 2 [Q8IXL7-2]	3	EBI-10174029,EBI-10699187
NEK6 - isoform 4 [Q9HC98-4]	3	EBI-10174029,EBI-11750983
PEF1 [Q9UBV8]	3	EBI-10174029,EBI-724639
PROP1 [O75360]	3	EBI-10174029,EBI-9027467
RAN [P62826]	3	EBI-10174029,EBI-286642
SARS1 [P49591]	3	EBI-10174029,EBI-1053431
SPAG8 - isoform 2 [Q99932-2]	3	EBI-10174029,EBI-11959123
SPRED1 [Q7Z699]	3	EBI-10174029,EBI-5235340
TRIP13 [Q15645]	6	EBI-10174029,EBI-358993
USHBP1 [Q8N6Y0]	3	EBI-10174029,EBI-739895
VAC14 [Q08AM6]	3	EBI-10174029,EBI-2107455

Protein-protein interaction databases

BioGRIDⁱ	128190, 225 interactors
IntActⁱ	A6NJ78, 41 interactors
STRINGⁱ	9606.ENSP00000384369

Miscellaneous databases

RNActⁱ	A6NJ78, protein

RNActⁱ

A6NJ78, protein

Structureⁱ

3D structure databases

SMRⁱ	A6NJ78
ModBaseⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	100 – 102	S-adenosyl-L-methionine bindingBy similarity		3

Sequence similaritiesⁱ

Belongs to the methyltransferase superfamily. RsmH family.Curated

Keywords - Domainⁱ

Transit peptide

Phylogenomic databases

eggNOGⁱ	KOG2782, Eukaryota
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000014756
HOGENOMⁱ	CLU_038422_1_0_1
InParanoidⁱ	A6NJ78
OMAⁱ	NPAKRTF
Database of Orthologous Groups More...OrthoDBⁱ	809593at2759
PhylomeDBⁱ	A6NJ78
TreeFamⁱ	TF106425

Family and domain databases

Gene3Dⁱ	1.10.150.170, 1 hit
HAMAPⁱ	MF_01007, 16SrRNA_methyltr_H, 1 hit
InterProⁱ	View protein in InterPro IPR002903, RsmH IPR023397, SAM-dep_MeTrfase_MraW_recog IPR029063, SAM-dependent_MTases
PANTHERⁱ	PTHR11265, PTHR11265, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01795, Methyltransf_5, 1 hit
SUPFAMⁱ	SSF53335, SSF53335, 1 hit SSF81799, SSF81799, 1 hit
TIGRFAMsⁱ	TIGR00006, TIGR00006, 1 hit

Sequences (4+)ⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsⁱ produced by alternative splicing. Align Add to basket

This entry has 4 described isoforms and 6 potential isoforms that are computationally mapped.Show all Align All

Isoform 1 (identifier: A6NJ78-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the canonicalⁱ sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLRYPYFCRM YKECLSCWLE SGIPNLGVWP NRIHTTAEKY REYEAREQTD 
        60         70         80         90        100
QTQAQELHRS QDRDFETMAK LHIPVMVDEV VHCLSPQKGQ IFLDMTFGSG 
       110        120        130        140        150
GHTKAILQKE SDIVLYALDR DPTAYALAEH LSELYPKQIR AMLGQFSQAE 
       160        170        180        190        200
ALLMKAGVQP GTFDGVLMDL GCSSMQLDTP ERGFSLRKDG PLDMRMDGGR 
       210        220        230        240        250
YPDMPTAADV VNALDQQALA SILRTYGEEK HAKKIASAIV QARSIYPITR 
       260        270        280        290        300
TQQLASIVAG AFPPSAIYTR KDLLQRSTHI ATKTFQALRI FVNNELNELY 
       310        320        330        340        350
TGLKTAQKFL RPGGRLVALS FHSLEDRIVK RFLLGISMTE RFNLSVRQQV 
       360        370        380        390        400
MKTSQLGSDH ENTEEVSMRR APLMWELIHK KVLSPQDQDV QDNPRGRSAK 

LRAAIKL

Length:407

Mass (Da):46,121

Last modified:July 24, 2007 - v1

Checksum:ⁱ1DA02B5842483080

GO

Isoform 2 (identifier: A6NJ78-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
260-273: GAFPPSAIYTRKDL → EYSETYFPVAQTLY
274-407: Missing.

« Hide

        10         20         30         40         50
MLRYPYFCRM YKECLSCWLE SGIPNLGVWP NRIHTTAEKY REYEAREQTD 
        60         70         80         90        100
QTQAQELHRS QDRDFETMAK LHIPVMVDEV VHCLSPQKGQ IFLDMTFGSG 
       110        120        130        140        150
GHTKAILQKE SDIVLYALDR DPTAYALAEH LSELYPKQIR AMLGQFSQAE 
       160        170        180        190        200
ALLMKAGVQP GTFDGVLMDL GCSSMQLDTP ERGFSLRKDG PLDMRMDGGR 
       210        220        230        240        250
YPDMPTAADV VNALDQQALA SILRTYGEEK HAKKIASAIV QARSIYPITR 
       260        270 
TQQLASIVAE YSETYFPVAQ TLY

Show »

Length:273

Mass (Da):30,872

Checksum:ⁱ71BBA524769D6066

GO

Isoform 3 (identifier: A6NJ78-3) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
91-99: IFLDMTFGS → SLGVRSVGI
100-407: Missing.

« Hide

        10         20         30         40         50
MLRYPYFCRM YKECLSCWLE SGIPNLGVWP NRIHTTAEKY REYEAREQTD 
        60         70         80         90 
QTQAQELHRS QDRDFETMAK LHIPVMVDEV VHCLSPQKGQ SLGVRSVGI

Show »

Length:99

Mass (Da):11,631

Checksum:ⁱ0A6B26342AB079D8

GO

Isoform 4 (identifier: A6NJ78-4) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
201-320: YPDMPTAADV...RPGGRLVALS → STGTCIYPKN...FPGSSHICEQ
321-407: Missing.

« Hide

        10         20         30         40         50
MLRYPYFCRM YKECLSCWLE SGIPNLGVWP NRIHTTAEKY REYEAREQTD 
        60         70         80         90        100
QTQAQELHRS QDRDFETMAK LHIPVMVDEV VHCLSPQKGQ IFLDMTFGSG 
       110        120        130        140        150
GHTKAILQKE SDIVLYALDR DPTAYALAEH LSELYPKQIR AMLGQFSQAE 
       160        170        180        190        200
ALLMKAGVQP GTFDGVLMDL GCSSMQLDTP ERGFSLRKDG PLDMRMDGGR 
       210        220        230        240        250
STGTCIYPKN IRGGEACQEN RFSNCSGTQH LPHHQNPAAC QHRCRSISSL 
       260        270 
CYLYTERLTT AIYPYCHQDF PGSSHICEQ

Show »

Length:279

Mass (Da):31,700

Checksum:ⁱB698284087F05A6E

GO

Computationally mapped potential isoform sequencesⁱ

There are 6 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

F8WD83	F8WD83_HUMAN	12S rRNA N4-methylcytidine (m4C) me... 12S rRNA N4-methylcytidine (m4C) methyltransferase	METTL15	164	Annotation score:
A0A0U1RR76	A0A0U1RR76_HUMAN	12S rRNA N4-methylcytidine (m4C) me... 12S rRNA N4-methylcytidine (m4C) methyltransferase	METTL15	274	Annotation score:
B5MC64	B5MC64_HUMAN	12S rRNA N4-methylcytidine (m4C) me... 12S rRNA N4-methylcytidine (m4C) methyltransferase	METTL15	93	Annotation score:
A0A0U1RR64	A0A0U1RR64_HUMAN	12S rRNA N4-methylcytidine (m4C) me... 12S rRNA N4-methylcytidine (m4C) methyltransferase	METTL15	107	Annotation score:
A0A0U1RRD2	A0A0U1RRD2_HUMAN	12S rRNA N4-methylcytidine (m4C) me... 12S rRNA N4-methylcytidine (m4C) methyltransferase	METTL15	149	Annotation score:
A0A0U1RRF2	A0A0U1RRF2_HUMAN	12S rRNA N4-methylcytidine (m4C) me... 12S rRNA N4-methylcytidine (m4C) methyltransferase	METTL15	90	Annotation score:

Sequence cautionⁱ

The sequence AAH30997 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	191	P → S in AAH30997 (PubMed:15489334).Curated		1
Sequence conflictⁱ	194	M → V in BAC03631 (PubMed:14702039).Curated		1

Natural variant

Feature key	Position(s)	DescriptionActions	Length
Natural variantⁱVAR_036801	31	N → K2 PublicationsCorresponds to variant dbSNP:rs2883478Ensembl.	1
Natural variantⁱVAR_036802	149	A → T. Corresponds to variant dbSNP:rs11823114Ensembl.	1
Natural variantⁱVAR_059446	267	I → F. Corresponds to variant dbSNP:rs11030280Ensembl.	1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Length
Alternative sequenceⁱVSP_028970	91 – 99	IFLDMTFGS → SLGVRSVGI in isoform 3. 1 Publication	9
Alternative sequenceⁱVSP_028971	100 – 407	Missing in isoform 3. 1 PublicationAdd BLAST	308
Alternative sequenceⁱVSP_028972	201 – 320	YPDMP…LVALS → STGTCIYPKNIRGGEACQEN RFSNCSGTQHLPHHQNPAAC QHRCRSISSLCYLYTERLTT AIYPYCHQDFPGSSHICEQ in isoform 4. 1 PublicationAdd BLAST	120
Alternative sequenceⁱVSP_028973	260 – 273	GAFPP…TRKDL → EYSETYFPVAQTLY in isoform 2. 1 PublicationAdd BLAST	14
Alternative sequenceⁱVSP_028974	274 – 407	Missing in isoform 2. 1 PublicationAdd BLAST	134
Alternative sequenceⁱVSP_028975	321 – 407	Missing in isoform 4. 1 PublicationAdd BLAST	87

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AK091298 mRNA Translation: BAC03631.1 AC023206 Genomic DNA No translation available. AC087376 Genomic DNA No translation available. CH471064 Genomic DNA Translation: EAW68267.1 CH471064 Genomic DNA Translation: EAW68266.1 BC030997 mRNA Translation: AAH30997.1 Different initiation. BC105287 mRNA Translation: AAI05288.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS31450.1 [A6NJ78-2] CCDS44559.1 [A6NJ78-1] CCDS73269.1 [A6NJ78-4]
NCBI Reference Sequences More...RefSeqⁱ	NP_001107000.1, NM_001113528.1 [A6NJ78-1] NP_001284704.1, NM_001297775.1 [A6NJ78-4] NP_689849.2, NM_152636.2 [A6NJ78-2] XP_016872786.1, XM_017017297.1 [A6NJ78-1]

Genome annotation databases

Ensemblⁱ	ENST00000303459; ENSP00000307251; ENSG00000169519 [A6NJ78-2] ENST00000406787; ENSP00000385507; ENSG00000169519 [A6NJ78-4] ENST00000407364; ENSP00000384369; ENSG00000169519 [A6NJ78-1] ENST00000437814; ENSP00000392806; ENSG00000169519 [A6NJ78-3]
GeneIDⁱ	196074
KEGGⁱ	hsa:196074
UCSC genome browser More...UCSCⁱ	uc001mse.3, human [A6NJ78-1]

Keywords - Coding sequence diversityⁱ

Alternative splicing

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
A6NJ78	12S rRNA N4-methylcytidine (m4C) methyltransferase		HUMAN		90	UniRef100_A6NJ78
+2

Protein	Similar proteins		Species	Score	Length	Source
A6NJ78	12S rRNA N4-methylcytidine methyltransferase		PONAB		407	UniRef90_A6NJ78
12S rRNA N4-methylcytidine (m4C) methyltransferase		HUMAN		90
Methyltransferase like 15		MANLE		450
Testis cDNA, clone: QtsA-14944, similar to human hypothetical protein FLJ33979 (FLJ33979)		MACFA		450
Methyltransferase like 15		COLAP		407
+33
A6NJ78-2	METTL15 isoform 12		PONAB		303	UniRef90_A6NJ78-2
Methyltransferase like 15		MACNE		274
METTL15 isoform 2		PANTR		274
Methyltransferase like 15		PANPA		274
Methyltransferase like 15		CERAT		274
+16
A6NJ78-3	12S rRNA N4-methylcytidine (m4C) methyltransferase		HUMAN		107	UniRef90_A6NJ78-3
Methyltransferase like 15		MACNE		107
Methyltransferase like 15		COLAP		107
Uncharacterized protein		NOMLE		106
Methyltransferase like 15		CERAT		106
+23
A6NJ78-4	Putative methyltransferase-like protein 15P1		HUMAN		234	UniRef90_A6NJ78-4
METTL15 isoform 1		PONAB		279
METTL15 isoform 4		PANTR		279
+7

Protein	Similar proteins		Species	Score	Length	Source
A6NJ78	12S rRNA N4-methylcytidine methyltransferase		PONAB		407	UniRef50_A6NJ78
12S rRNA N4-methylcytidine (m4C) methyltransferase		HUMAN		90
Methyltransferase like 15		MACFA		450
Methyltransferase like 15		MANLE		450
Putative S-adenosyl-L-methionine-dependent methyltransferase METT5D1		MACMU		407
+125
A6NJ78-2	METTL15 isoform 12		PONAB		303	UniRef50_A6NJ78-2
Methyltransferase like 15		MACNE		274
12S rRNA N4-methylcytidine (m4C) methyltransferase		HUMAN		274
Methyltransferase like 15		PANPA		274
Methyltransferase like 15		CERAT		274
+97
A6NJ78-3	Methyltransferase like 15		COLAP		107	UniRef50_A6NJ78-3
Methyltransferase like 15		MACNE		107
12S rRNA N4-methylcytidine (m4C) methyltransferase		HUMAN		107
Methyltransferase like 15		CERAT		106
Methyltransferase like 15		MANLE		106
+23
A6NJ78-4	Putative methyltransferase-like protein 15P1		HUMAN		234	UniRef50_A6NJ78-4
METTL15 isoform 4		PANTR		279
METTL15 isoform 1		PONAB		279
Methyltransferase like 15		pale spear-nosed bat		270
Methyltransferase like 15		SAIBB		279
+28

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AK091298 mRNA Translation: BAC03631.1 AC023206 Genomic DNA No translation available. AC087376 Genomic DNA No translation available. CH471064 Genomic DNA Translation: EAW68267.1 CH471064 Genomic DNA Translation: EAW68266.1 BC030997 mRNA Translation: AAH30997.1 Different initiation. BC105287 mRNA Translation: AAI05288.1
CCDSⁱ	CCDS31450.1 [A6NJ78-2] CCDS44559.1 [A6NJ78-1] CCDS73269.1 [A6NJ78-4]
RefSeqⁱ	NP_001107000.1, NM_001113528.1 [A6NJ78-1] NP_001284704.1, NM_001297775.1 [A6NJ78-4] NP_689849.2, NM_152636.2 [A6NJ78-2] XP_016872786.1, XM_017017297.1 [A6NJ78-1]

3D structure databases

SMRⁱ	A6NJ78
ModBaseⁱ	Search...

Protein-protein interaction databases

BioGRIDⁱ	128190, 225 interactors
IntActⁱ	A6NJ78, 41 interactors
STRINGⁱ	9606.ENSP00000384369

PTM databases

GlyGenⁱ	A6NJ78, 1 site, 1 O-linked glycan (1 site)
iPTMnetⁱ	A6NJ78
PhosphoSitePlusⁱ	A6NJ78

Genetic variation databases

BioMutaⁱ	METTL15

BioMutaⁱ

METTL15

Proteomic databases

EPDⁱ	A6NJ78
jPOSTⁱ	A6NJ78
MassIVEⁱ	A6NJ78
MaxQBⁱ	A6NJ78
PaxDbⁱ	A6NJ78
PeptideAtlasⁱ	A6NJ78
PRIDEⁱ	A6NJ78
ProteomicsDBⁱ	1313 [A6NJ78-1] 1314 [A6NJ78-2] 1315 [A6NJ78-3] 1316 [A6NJ78-4]

Protocols and materials databases

Antibodypediaⁱ	25483, 44 antibodies
The DNASU plasmid repository More...DNASUⁱ	196074

Genome annotation databases

Ensemblⁱ	ENST00000303459; ENSP00000307251; ENSG00000169519 [A6NJ78-2] ENST00000406787; ENSP00000385507; ENSG00000169519 [A6NJ78-4] ENST00000407364; ENSP00000384369; ENSG00000169519 [A6NJ78-1] ENST00000437814; ENSP00000392806; ENSG00000169519 [A6NJ78-3]
GeneIDⁱ	196074
KEGGⁱ	hsa:196074
UCSCⁱ	uc001mse.3, human [A6NJ78-1]

Organism-specific databases

CTDⁱ	196074
DisGeNETⁱ	196074
GeneCardsⁱ	METTL15
HGNCⁱ	HGNC:26606, METTL15
HPAⁱ	ENSG00000169519, Low tissue specificity
MIMⁱ	618711, gene
neXtProtⁱ	NX_A6NJ78
OpenTargetsⁱ	ENSG00000169519
PharmGKBⁱ	PA142671458
VEuPathDBⁱ	HostDB:ENSG00000169519
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG2782, Eukaryota
GeneTreeⁱ	ENSGT00390000014756
HOGENOMⁱ	CLU_038422_1_0_1
InParanoidⁱ	A6NJ78
OMAⁱ	NPAKRTF
OrthoDBⁱ	809593at2759
PhylomeDBⁱ	A6NJ78
TreeFamⁱ	TF106425

Enzyme and pathway databases

PathwayCommonsⁱ	A6NJ78

PathwayCommonsⁱ

A6NJ78

Miscellaneous databases

BioGRID-ORCSⁱ	196074, 76 hits in 986 CRISPR screens
ChiTaRSⁱ	METTL15, human
GenomeRNAiⁱ	196074
Pharosⁱ	A6NJ78, Tdark
Protein Ontology More...PROⁱ	PR:A6NJ78
RNActⁱ	A6NJ78, protein
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000169519, Expressed in adrenal tissue and 197 other tissues
ExpressionAtlasⁱ	A6NJ78, baseline and differential
Genevisibleⁱ	A6NJ78, HS

Family and domain databases

Gene3Dⁱ	1.10.150.170, 1 hit
HAMAPⁱ	MF_01007, 16SrRNA_methyltr_H, 1 hit
InterProⁱ	View protein in InterPro IPR002903, RsmH IPR023397, SAM-dep_MeTrfase_MraW_recog IPR029063, SAM-dependent_MTases
PANTHERⁱ	PTHR11265, PTHR11265, 1 hit
Pfamⁱ	View protein in Pfam PF01795, Methyltransf_5, 1 hit
SUPFAMⁱ	SSF53335, SSF53335, 1 hit SSF81799, SSF81799, 1 hit
TIGRFAMsⁱ	TIGR00006, TIGR00006, 1 hit
MobiDBⁱ	Search...

Entry informationⁱ

Entry nameⁱ	MET15_HUMAN
Accessionⁱ	A6NJ78Primary (citable) accession number: A6NJ78 Secondary accession number(s): A8MRS5 , B7WNU2, Q3MHD3, Q8N601, Q8NBA7
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	October 23, 2007
	Last sequence update:	July 24, 2007
	Last modified:	September 29, 2021
	This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

Reference proteome

Documents

Human chromosome 11
Human chromosome 11: entries, gene names and cross-references to MIM
Human entries with genetic variants
List of human entries with genetic variants
Human variants curated from literature reports
Index of human variants curated from literature reports
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
SIMILARITY comments
Index of protein domains and families

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UniProtKB - A6NJ78 (MET15_HUMAN)

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12S rRNA N4-methylcytidine (m4C) methyltransferase

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Isoform 4 [A6NJ78-4]

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Functionⁱ

Catalytic activityⁱ

GO - Molecular functionⁱ

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Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

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