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Protein

Calpain-8

Gene

CAPN8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Calcium-regulated non-lysosomal thiol-protease. Involved in membrane trafficking in the gastric surface mucus cells (pit cells) and may involve the membrane trafficking of mucus cells via interactions with coat protein. Proteolytically cleaves the beta-subunit of coatomer complex (By similarity).By similarity

Catalytic activityi

Broad endopeptidase specificity.

Cofactori

Ca2+CuratedNote: Binds 2 calcium ions.Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei105By similarity1
Active sitei262By similarity1
Active sitei286By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi588 – 5991PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi618 – 6292PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

  • calcium-dependent cysteine-type endopeptidase activity Source: GO_Central
  • calcium ion binding Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
LigandCalcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-HSA-1474228 Degradation of the extracellular matrix

Protein family/group databases

MEROPSiC02.007

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain-8 (EC:3.4.22.53)
Alternative name(s):
New calpain 2
Short name:
nCL-2
Stomach-specific M-type calpain
Gene namesi
Name:CAPN8
Synonyms:NCL2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000203697.11
HGNCiHGNC:1485 CAPN8
neXtProtiNX_A6NHC0

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000203697

Polymorphism and mutation databases

BioMutaiCAPN8

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003492801 – 703Calpain-8Add BLAST703

Post-translational modificationi

Undergoes autolytic cleavage between Ala-5 and Ala-6 which gives rise to fragments extending from Ala-6 to the C-terminus, Ala-6 to the EF-hand 2 domain and from Ala-6 to the beginning of domain III.By similarity

Keywords - PTMi

Autocatalytic cleavage

Proteomic databases

MaxQBiA6NHC0
PRIDEiA6NHC0
ProteomicsDBi1188

PTM databases

iPTMnetiA6NHC0
PhosphoSitePlusiA6NHC0

Expressioni

Tissue specificityi

Stomach.

Gene expression databases

BgeeiENSG00000203697 Expressed in 114 organ(s), highest expression level in fundus of stomach
CleanExiHS_CAPN8
ExpressionAtlasiA6NHC0 baseline and differential
GenevisibleiA6NHC0 HS

Organism-specific databases

HPAiHPA073939

Interactioni

Subunit structurei

Monomer and homooligomer. Interacts with COPS1/GPS1, COPB1, EYA2, NME2, NME4 and TOMM70 (By similarity).By similarity

Protein-protein interaction databases

BioGridi132835, 4 interactors

Structurei

3D structure databases

ProteinModelPortaliA6NHC0
SMRiA6NHC0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini45 – 344Calpain catalyticPROSITE-ProRule annotationAdd BLAST300
Domaini575 – 610EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini605 – 640EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini670 – 703EF-hand 3PROSITE-ProRule annotationAdd BLAST34

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni356 – 379Domain IIIAdd BLAST24

Domaini

The domain III mediates oligomerization.By similarity

Sequence similaritiesi

Belongs to the peptidase C2 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00760000118971
HOGENOMiHOG000232035
HOVERGENiHBG012645
InParanoidiA6NHC0
KOiK08577
OMAiDGEFCLR
OrthoDBiEOG091G049E
PhylomeDBiA6NHC0
TreeFamiTF314748

Family and domain databases

CDDicd00214 Calpain_III, 1 hit
cd00044 CysPc, 1 hit
InterProiView protein in InterPro
IPR033883 C2_III
IPR022684 Calpain_cysteine_protease
IPR022682 Calpain_domain_III
IPR022683 Calpain_III
IPR036213 Calpain_III_sf
IPR029543 CAPN8
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
IPR038765 Papain_like_cys_pep_sf
IPR000169 Pept_cys_AS
IPR001300 Peptidase_C2_calpain_cat
PANTHERiPTHR10183:SF374 PTHR10183:SF374, 1 hit
PfamiView protein in Pfam
PF01067 Calpain_III, 1 hit
PF00648 Peptidase_C2, 1 hit
PRINTSiPR00704 CALPAIN
SMARTiView protein in SMART
SM00720 calpain_III, 1 hit
SM00230 CysPc, 1 hit
SUPFAMiSSF47473 SSF47473, 1 hit
SSF49758 SSF49758, 1 hit
SSF54001 SSF54001, 1 hit
PROSITEiView protein in PROSITE
PS50203 CALPAIN_CAT, 1 hit
PS00018 EF_HAND_1, 2 hits
PS50222 EF_HAND_2, 3 hits
PS00139 THIOL_PROTEASE_CYS, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 7 potential isoforms that are computationally mapped.Show allAlign All

A6NHC0-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAAQAAGVSR QRAATQGLGS NQNALKYLGQ DFKTLRQQCL DSGVLFKDPE
60 70 80 90 100
FPACPSALGY KDLGPGSPQT QGIIWKRPTE LCPSPQFIVG GATRTDICQG
110 120 130 140 150
GLGDCWLLAA IASLTLNEEL LYRVVPRDQD FQENYAGIFH FQFWQYGEWV
160 170 180 190 200
EVVIDDRLPT KNGQLLFLHS EQGNEFWSAL LEKAYAKLNG CYEALAGGST
210 220 230 240 250
VEGFEDFTGG ISEFYDLKKP PANLYQIIRK ALCAGSLLGC SIDVSSAAEA
260 270 280 290 300
EAITSQKLVK SHAYSVTGVE EVNFQGHPEK LIRLRNPWGE VEWSGAWSDD
310 320 330 340 350
APEWNHIDPR RKEELDKKVE DGEFWMSLSD FVRQFSRLEI CNLSPDSLSS
360 370 380 390 400
EEVHKWNLVL FNGHWTRGST AGGCQNYPAT YWTNPQFKIR LDEVDEDQEE
410 420 430 440 450
SIGEPCCTVL LGLMQKNRRW RKRIGQGMLS IGYAVYQVPK ELESHTDAHL
460 470 480 490 500
GRDFFLAYQP SARTSTYVNL REVSGRARLP PGEYLVVPST FEPFKDGEFC
510 520 530 540 550
LRVFSEKKAQ ALEIGDVVAG NPYEPHPSEV DQEDDQFRRL FEKLAGKDSE
560 570 580 590 600
ITANALKILL NEAFSKRTDI KFDGFNINTC REMISLLDSN GTGTLGAVEF
610 620 630 640 650
KTLWLKIQKY LEIYWETDYN HSGTIDAHEM RTALRKAGFT LNSQVQQTIA
660 670 680 690 700
LRYACSKLGI NFDSFVACMI RLETLFKLFS LLDEDKDGMV QLSLAEWLCC

VLV
Length:703
Mass (Da):79,144
Last modified:August 10, 2010 - v3
Checksum:i2A6DFFC0814894A8
GO

Computationally mapped potential isoform sequencesi

There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
B1B154B1B154_HUMAN
Calpain-8
CAPN8
381Annotation score:
H0YEJ4H0YEJ4_HUMAN
Calpain-8
CAPN8
173Annotation score:
B1B153B1B153_HUMAN
Calpain-8
CAPN8
437Annotation score:
A3QK37A3QK37_HUMAN
Calpain-8
CAPN8
156Annotation score:
A3QK36A3QK36_HUMAN
Calpain-8
CAPN8
101Annotation score:
H0YDN4H0YDN4_HUMAN
Calpain-8
CAPN8 hCG_2036808
38Annotation score:
H0YEQ4H0YEQ4_HUMAN
Calpain-8
CAPN8
20Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti245S → Y in AAI57894 (PubMed:15489334).Curated1
Sequence conflicti592T → M in AAI57894 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC099065 Genomic DNA No translation available.
CR628412 Genomic DNA No translation available.
CR848842 Genomic DNA No translation available.
BC157893 mRNA Translation: AAI57894.1
CCDSiCCDS73038.1
RefSeqiNP_001137434.1, NM_001143962.1
UniGeneiHs.291487

Genome annotation databases

EnsembliENST00000366872; ENSP00000355837; ENSG00000203697
GeneIDi388743
KEGGihsa:388743
UCSCiuc031vow.1 human

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC099065 Genomic DNA No translation available.
CR628412 Genomic DNA No translation available.
CR848842 Genomic DNA No translation available.
BC157893 mRNA Translation: AAI57894.1
CCDSiCCDS73038.1
RefSeqiNP_001137434.1, NM_001143962.1
UniGeneiHs.291487

3D structure databases

ProteinModelPortaliA6NHC0
SMRiA6NHC0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi132835, 4 interactors

Protein family/group databases

MEROPSiC02.007

PTM databases

iPTMnetiA6NHC0
PhosphoSitePlusiA6NHC0

Polymorphism and mutation databases

BioMutaiCAPN8

Proteomic databases

MaxQBiA6NHC0
PRIDEiA6NHC0
ProteomicsDBi1188

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000366872; ENSP00000355837; ENSG00000203697
GeneIDi388743
KEGGihsa:388743
UCSCiuc031vow.1 human

Organism-specific databases

CTDi388743
EuPathDBiHostDB:ENSG00000203697.11
GeneCardsiCAPN8
HGNCiHGNC:1485 CAPN8
HPAiHPA073939
neXtProtiNX_A6NHC0
OpenTargetsiENSG00000203697
GenAtlasiSearch...

Phylogenomic databases

GeneTreeiENSGT00760000118971
HOGENOMiHOG000232035
HOVERGENiHBG012645
InParanoidiA6NHC0
KOiK08577
OMAiDGEFCLR
OrthoDBiEOG091G049E
PhylomeDBiA6NHC0
TreeFamiTF314748

Enzyme and pathway databases

ReactomeiR-HSA-1474228 Degradation of the extracellular matrix

Miscellaneous databases

ChiTaRSiCAPN8 human
GenomeRNAii388743
PROiPR:A6NHC0

Gene expression databases

BgeeiENSG00000203697 Expressed in 114 organ(s), highest expression level in fundus of stomach
CleanExiHS_CAPN8
ExpressionAtlasiA6NHC0 baseline and differential
GenevisibleiA6NHC0 HS

Family and domain databases

CDDicd00214 Calpain_III, 1 hit
cd00044 CysPc, 1 hit
InterProiView protein in InterPro
IPR033883 C2_III
IPR022684 Calpain_cysteine_protease
IPR022682 Calpain_domain_III
IPR022683 Calpain_III
IPR036213 Calpain_III_sf
IPR029543 CAPN8
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
IPR038765 Papain_like_cys_pep_sf
IPR000169 Pept_cys_AS
IPR001300 Peptidase_C2_calpain_cat
PANTHERiPTHR10183:SF374 PTHR10183:SF374, 1 hit
PfamiView protein in Pfam
PF01067 Calpain_III, 1 hit
PF00648 Peptidase_C2, 1 hit
PRINTSiPR00704 CALPAIN
SMARTiView protein in SMART
SM00720 calpain_III, 1 hit
SM00230 CysPc, 1 hit
SUPFAMiSSF47473 SSF47473, 1 hit
SSF49758 SSF49758, 1 hit
SSF54001 SSF54001, 1 hit
PROSITEiView protein in PROSITE
PS50203 CALPAIN_CAT, 1 hit
PS00018 EF_HAND_1, 2 hits
PS50222 EF_HAND_2, 3 hits
PS00139 THIOL_PROTEASE_CYS, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCAN8_HUMAN
AccessioniPrimary (citable) accession number: A6NHC0
Secondary accession number(s): B2RXL2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: August 10, 2010
Last modified: September 12, 2018
This is version 94 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  3. Peptidase families
    Classification of peptidase families and list of entries
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Main funding by: National Institutes of Health

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