UniProtKB - A6NFQ2 (TCAF2_HUMAN)
Protein
TRPM8 channel-associated factor 2
Gene
TCAF2
Organism
Homo sapiens (Human)
Status
Functioni
Negatively regulates the plasma membrane cation channel TRPM8 activity. Involved in the recruitment of TRPM8 to the cell surface. Promotes prostate cancer cell migration stimulation in a TRPM8-dependent manner.1 Publication
GO - Molecular functioni
- ion channel binding Source: MGI
GO - Biological processi
- negative regulation of anion channel activity Source: UniProtKB
- positive regulation of cell migration Source: UniProtKB
- positive regulation of protein targeting to membrane Source: UniProtKB
- regulation of anion channel activity Source: GO_Central
Keywordsi
Biological process | Transport |
Enzyme and pathway databases
PathwayCommonsi | A6NFQ2 |
Names & Taxonomyi
Protein namesi | Recommended name: TRPM8 channel-associated factor 21 PublicationAlternative name(s): TRP channel-associated factor 21 Publication |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:26878, TCAF2 |
MIMi | 616252, gene |
neXtProti | NX_A6NFQ2 |
VEuPathDBi | HostDB:ENSG00000170379.19 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication
Note: Colocalizes with TRPM8 on the plasma membrane.1 Publication
Plasma Membrane
- plasma membrane Source: UniProtKB
Other locations
- cell junction Source: HPA
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Organism-specific databases
OpenTargetsi | ENSG00000170379 |
PharmGKBi | PA162385772 |
Miscellaneous databases
Pharosi | A6NFQ2, Tbio |
Genetic variation databases
BioMutai | TCAF2 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000320187 | 1 – 919 | TRPM8 channel-associated factor 2Add BLAST | 919 |
Proteomic databases
EPDi | A6NFQ2 |
jPOSTi | A6NFQ2 |
MassIVEi | A6NFQ2 |
MaxQBi | A6NFQ2 |
PaxDbi | A6NFQ2 |
PeptideAtlasi | A6NFQ2 |
PRIDEi | A6NFQ2 |
ProteomicsDBi | 1064 [A6NFQ2-1] 1065 [A6NFQ2-2] 1066 [A6NFQ2-3] |
PTM databases
iPTMneti | A6NFQ2 |
PhosphoSitePlusi | A6NFQ2 |
Expressioni
Tissue specificityi
Isoform 2 is expressed in the prostate and in cancerous prostate samples.1 Publication
Gene expression databases
Bgeei | ENSG00000170379, Expressed in islet of Langerhans and 111 other tissues |
ExpressionAtlasi | A6NFQ2, baseline and differential |
Genevisiblei | A6NFQ2, HS |
Organism-specific databases
HPAi | ENSG00000170379, Low tissue specificity |
Interactioni
Subunit structurei
Isoform 2 interacts with TRPM8 (via N-terminus and C-terminus domains); the interaction inhibits TRPM8 channel activity.
Interacts with TRPV6.
1 PublicationGO - Molecular functioni
- ion channel binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 130260, 26 interactors |
IntActi | A6NFQ2, 20 interactors |
STRINGi | 9606.ENSP00000412724 |
Miscellaneous databases
RNActi | A6NFQ2, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 542 – 841 | Peptidase M60PROSITE-ProRule annotationAdd BLAST | 300 |
Sequence similaritiesi
Belongs to the TCAF family.Curated
Phylogenomic databases
eggNOGi | ENOG502QQUS, Eukaryota |
GeneTreei | ENSGT00390000017365 |
HOGENOMi | CLU_011215_0_0_1 |
InParanoidi | A6NFQ2 |
OMAi | AHEGMLC |
OrthoDBi | 1049811at2759 |
PhylomeDBi | A6NFQ2 |
TreeFami | TF331520 |
Family and domain databases
Gene3Di | 1.10.390.30, 1 hit |
InterProi | View protein in InterPro IPR029062, Class_I_gatase-like IPR035423, M60-like_N IPR042279, Pep_M60_3 IPR031161, Peptidase_M60_dom |
Pfami | View protein in Pfam PF17291, M60-like_N, 1 hit PF13402, Peptidase_M60, 1 hit |
SMARTi | View protein in SMART SM01276, M60-like, 1 hit |
SUPFAMi | SSF52317, SSF52317, 1 hit |
PROSITEi | View protein in PROSITE PS51723, PEPTIDASE_M60, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: A6NFQ2-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MATIAAAAFE ALMDGVTCWD VPRGPIPSEL LLIGEAAFPV MVNDKGQVLI
60 70 80 90 100
AASSYGRGRL VVVSHEGYLS HTGLAPFLLN AVSWLCPCPG APVGVHPSLA
110 120 130 140 150
PLVNILQDAG LEAQVKPEPG EPLGVYCINA YNDTLTATLI QFVKHGGGLL
160 170 180 190 200
IGGQAWYWAS QHGPDKVLSR FPGNKVTSVA GVYFTDTYGD RDRFKVSKKV
210 220 230 240 250
PKIPLHVRYG EDVRQDQQQL LEGISELDIR TGGVPSQLLV HGALAFPLGL
260 270 280 290 300
DASLNCFLAA AHYGRGRVVL AAHECLLCAP KMGPFLLNAV RWLARGQTGK
310 320 330 340 350
VGVNTNLKDL CPLLSEHGLQ CSLEPHLNSD LCVYCCKAYS DKEAKQLQEF
360 370 380 390 400
VAEGGGLLIG GQAWWWASQN PGHCPLAGFP GNIILNCFGL SILPQTLKAG
410 420 430 440 450
CFPVPTPEMR SYHFRKALSQ FQAILNHENG NLEKSCLAKL RVDGAAFLQI
460 470 480 490 500
PAEGVPAYIS LHRLLRKMLR GSGLPAVSRE NPVASDSYEA AVLSLATGLA
510 520 530 540 550
HSGTDCSQLA QGLGTWTCSS SLYPSKHPIT VEINGINPGN NDCWVSTGLY
560 570 580 590 600
LLEGQNAEVS LSEAAASAGL RVQIGCHTDD LTKARKLSRA PVVTHQCWMD
610 620 630 640 650
RTERSVSCLW GGLLYVIVPK GSQLGPVPVT IRGAVPAPYY KLGKTSLEEW
660 670 680 690 700
KRQMQENLAP WGELATDNII LTVPTTNLQA LKDPEPVLRL WDEMMQAVAR
710 720 730 740 750
LAAEPFPFRR PERIVADVQI SAGWMHSGYP IMCHLESVKE IINEMDMRSR
760 770 780 790 800
GVWGPIHELG HNQQRHGWEF PPHTTEATCN LWSVYVHETV LGIPRAQAHE
810 820 830 840 850
ALSPPERERR IKAHLGKGAP LCDWNVWTAL ETYLQLQEAF GWEPFTQLFA
860 870 880 890 900
EYQTLSHLPK DNTGRMNLWV KKFSEKVKKN LVPFFEAWGW PIQKEVADSL
910
ASLPEWQENP MQVYLRARK
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketQ8N1I1 | Q8N1I1_HUMAN | TRPM8 channel-associated factor 2 | TCAF2 | 564 | Annotation score: |
Sequence cautioni
The sequence BAC03376 differs from that shown. Reason: Erroneous initiation.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 72 | T → A in AAN87343 (Ref. 1) Curated | 1 | |
Sequence conflicti | 72 | T → A in AAI17234 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 592 | V → M in AAI17234 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 592 | V → M in BAC03376 (PubMed:12693554).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_042050 | 1 – 207 | MATIA…IPLHV → MKIFQKEFKFILKKCTPIQK YREENGNSSSSPHHQLQSHA SGP in isoform 3. 1 PublicationAdd BLAST | 207 | |
Alternative sequenceiVSP_042051 | 538 | P → PESHSVIQVGMQWRDLSSCN LHLLGLSNSSLSASCVAGTT GTRHHAWLIFVFLVEREFHR K in isoform 3. 1 Publication | 1 | |
Alternative sequenceiVSP_031635 | 836 – 919 | LQEAF…LRARK → VLSRNSGRRG in isoform 2. 2 PublicationsAdd BLAST | 84 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY167570 mRNA Translation: AAN87343.1 AK296318 mRNA Translation: BAG59014.1 AC073264 Genomic DNA No translation available. AC106862 Genomic DNA No translation available. CH236959 Genomic DNA Translation: EAL23793.1 BC113530 mRNA Translation: AAI13531.1 BC117233 mRNA Translation: AAI17234.1 AK090395 mRNA Translation: BAC03376.1 Different initiation. |
CCDSi | CCDS34769.1 [A6NFQ2-2] CCDS47735.2 [A6NFQ2-3] CCDS87559.1 [A6NFQ2-1] |
RefSeqi | NP_001123497.1, NM_001130025.1 NP_001123498.2, NM_001130026.2 [A6NFQ2-3] NP_775949.2, NM_173678.2 [A6NFQ2-2] XP_006715990.1, XM_006715927.3 XP_006715991.1, XM_006715928.3 [A6NFQ2-2] |
Genome annotation databases
Ensembli | ENST00000357344; ENSP00000349902; ENSG00000170379 [A6NFQ2-2] ENST00000411935; ENSP00000389100; ENSG00000170379 [A6NFQ2-3] ENST00000425618; ENSP00000441099; ENSG00000170379 [A6NFQ2-3] ENST00000441159; ENSP00000404265; ENSG00000170379 [A6NFQ2-1] ENST00000444908; ENSP00000412724; ENSG00000170379 [A6NFQ2-2] ENST00000643242; ENSP00000495809; ENSG00000170379 [A6NFQ2-2] |
GeneIDi | 285966 |
KEGGi | hsa:285966 |
UCSCi | uc003wdf.4, human [A6NFQ2-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY167570 mRNA Translation: AAN87343.1 AK296318 mRNA Translation: BAG59014.1 AC073264 Genomic DNA No translation available. AC106862 Genomic DNA No translation available. CH236959 Genomic DNA Translation: EAL23793.1 BC113530 mRNA Translation: AAI13531.1 BC117233 mRNA Translation: AAI17234.1 AK090395 mRNA Translation: BAC03376.1 Different initiation. |
CCDSi | CCDS34769.1 [A6NFQ2-2] CCDS47735.2 [A6NFQ2-3] CCDS87559.1 [A6NFQ2-1] |
RefSeqi | NP_001123497.1, NM_001130025.1 NP_001123498.2, NM_001130026.2 [A6NFQ2-3] NP_775949.2, NM_173678.2 [A6NFQ2-2] XP_006715990.1, XM_006715927.3 XP_006715991.1, XM_006715928.3 [A6NFQ2-2] |
3D structure databases
SMRi | A6NFQ2 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 130260, 26 interactors |
IntActi | A6NFQ2, 20 interactors |
STRINGi | 9606.ENSP00000412724 |
PTM databases
iPTMneti | A6NFQ2 |
PhosphoSitePlusi | A6NFQ2 |
Genetic variation databases
BioMutai | TCAF2 |
Proteomic databases
EPDi | A6NFQ2 |
jPOSTi | A6NFQ2 |
MassIVEi | A6NFQ2 |
MaxQBi | A6NFQ2 |
PaxDbi | A6NFQ2 |
PeptideAtlasi | A6NFQ2 |
PRIDEi | A6NFQ2 |
ProteomicsDBi | 1064 [A6NFQ2-1] 1065 [A6NFQ2-2] 1066 [A6NFQ2-3] |
Protocols and materials databases
Antibodypediai | 51007, 38 antibodies |
DNASUi | 285966 |
Genome annotation databases
Ensembli | ENST00000357344; ENSP00000349902; ENSG00000170379 [A6NFQ2-2] ENST00000411935; ENSP00000389100; ENSG00000170379 [A6NFQ2-3] ENST00000425618; ENSP00000441099; ENSG00000170379 [A6NFQ2-3] ENST00000441159; ENSP00000404265; ENSG00000170379 [A6NFQ2-1] ENST00000444908; ENSP00000412724; ENSG00000170379 [A6NFQ2-2] ENST00000643242; ENSP00000495809; ENSG00000170379 [A6NFQ2-2] |
GeneIDi | 285966 |
KEGGi | hsa:285966 |
UCSCi | uc003wdf.4, human [A6NFQ2-1] |
Organism-specific databases
CTDi | 285966 |
GeneCardsi | TCAF2 |
HGNCi | HGNC:26878, TCAF2 |
HPAi | ENSG00000170379, Low tissue specificity |
MIMi | 616252, gene |
neXtProti | NX_A6NFQ2 |
OpenTargetsi | ENSG00000170379 |
PharmGKBi | PA162385772 |
VEuPathDBi | HostDB:ENSG00000170379.19 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502QQUS, Eukaryota |
GeneTreei | ENSGT00390000017365 |
HOGENOMi | CLU_011215_0_0_1 |
InParanoidi | A6NFQ2 |
OMAi | AHEGMLC |
OrthoDBi | 1049811at2759 |
PhylomeDBi | A6NFQ2 |
TreeFami | TF331520 |
Enzyme and pathway databases
PathwayCommonsi | A6NFQ2 |
Miscellaneous databases
BioGRID-ORCSi | 285966, 2 hits in 849 CRISPR screens |
GenomeRNAii | 285966 |
Pharosi | A6NFQ2, Tbio |
PROi | PR:A6NFQ2 |
RNActi | A6NFQ2, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000170379, Expressed in islet of Langerhans and 111 other tissues |
ExpressionAtlasi | A6NFQ2, baseline and differential |
Genevisiblei | A6NFQ2, HS |
Family and domain databases
Gene3Di | 1.10.390.30, 1 hit |
InterProi | View protein in InterPro IPR029062, Class_I_gatase-like IPR035423, M60-like_N IPR042279, Pep_M60_3 IPR031161, Peptidase_M60_dom |
Pfami | View protein in Pfam PF17291, M60-like_N, 1 hit PF13402, Peptidase_M60, 1 hit |
SMARTi | View protein in SMART SM01276, M60-like, 1 hit |
SUPFAMi | SSF52317, SSF52317, 1 hit |
PROSITEi | View protein in PROSITE PS51723, PEPTIDASE_M60, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TCAF2_HUMAN | |
Accessioni | A6NFQ2Primary (citable) accession number: A6NFQ2 Secondary accession number(s): B4DK02 Q8NF84 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 26, 2008 |
Last sequence update: | January 15, 2008 | |
Last modified: | February 10, 2021 | |
This is version 118 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Human chromosome 7
Human chromosome 7: entries, gene names and cross-references to MIM