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Protein

Sperm-egg fusion protein Juno

Gene

IZUMO1R

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Receptor for IZUMO1 present at the cell surface of oocytes (oolemma), which is essential for species-specific gamete recognition and fertilization. The IZUMO1:IZUMO1R/JUNO interaction is a necessary adhesion event between sperm and egg that is required for fertilization but is not sufficient for cell fusion. The ligand-receptor interaction probably does not act as a membrane 'fusogen'. Does not bind folate.By similarity

GO - Molecular functioni

  • signaling receptor activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionReceptor
Biological processFertilization

Enzyme and pathway databases

ReactomeiR-HSA-163125 Post-translational modification: synthesis of GPI-anchored proteins

Protein family/group databases

TCDBi9.B.92.1.2 the folate receptor (fr) family

Names & Taxonomyi

Protein namesi
Recommended name:
Sperm-egg fusion protein Juno
Alternative name(s):
Folate receptor 4
Folate receptor delta
Short name:
FR-delta
IZUMO1 receptor protein JUNO1 Publication
Gene namesi
Name:IZUMO1RImported
Synonyms:FOLR4, JUNO
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

EuPathDBiHostDB:ENSG00000183560.8
HGNCiHGNC:32565 IZUMO1R
MIMi615737 gene
neXtProtiNX_A6ND01

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi45E → A: Nearly abolishes interaction with IZUMO1. 1 Publication1
Mutagenesisi45E → K: Abolishes interaction with IZUMO1. 1 Publication1
Mutagenesisi62W → A: Nearly abolishes interaction with IZUMO1. 2 Publications1
Mutagenesisi81L → A: Abolishes interaction with IZUMO1. 2 Publications1
Mutagenesisi163K → E: Mildly decreases interaction with IZUMO1. 1 Publication1

Organism-specific databases

OpenTargetsiENSG00000183560

Polymorphism and mutation databases

BioMutaiFOLR4

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000033298720 – 228Sperm-egg fusion protein JunoAdd BLAST209
PropeptideiPRO_0000429472229 – 250Sequence analysisAdd BLAST22

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi27 ↔ 55Combined sources2 Publications
Disulfide bondi47 ↔ 95Combined sources2 Publications
Disulfide bondi56 ↔ 99Combined sources2 Publications
Glycosylationi73N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi79 ↔ 172Combined sources2 Publications
Disulfide bondi86 ↔ 143Combined sources2 Publications
Disulfide bondi132 ↔ 206Combined sources2 Publications
Disulfide bondi136 ↔ 186Combined sources2 Publications
Disulfide bondi149 ↔ 166Combined sources2 Publications
Lipidationi228GPI-anchor amidated serineSequence analysis1

Post-translational modificationi

The protein is rapidly cleaved following fertilization, being only weakly detectable in zona-intact fertilized eggs at telophase II and undetectable at the pronuclear stage. Sheding is probably required to block to polyspermy and ensuring egg fusion with a single sperm.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiA6ND01
PRIDEiA6ND01
ProteomicsDBi872
873 [A6ND01-2]

Expressioni

Gene expression databases

BgeeiENSG00000183560 Expressed in 33 organ(s), highest expression level in lymph node
ExpressionAtlasiA6ND01 baseline and differential

Interactioni

Subunit structurei

Monomer (PubMed:27309808). Interacts with IZUMO1; the interaction is direct. IZUMO1 and IZUMO1R/JUNO form a complex with 1:1 stoichiometry (PubMed:24739963, PubMed:27309818, PubMed:27309808).3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
IZUMO1Q8IYV9-113EBI-16208304,EBI-16208297

Protein-protein interaction databases

DIPiDIP-62034N
IntActiA6ND01, 1 interactor
STRINGi9606.ENSP00000416935

Structurei

Secondary structure

1250
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliA6ND01
SMRiA6ND01
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni62 – 81Important for interaction with IZUMO12 PublicationsAdd BLAST20

Sequence similaritiesi

Belongs to the folate receptor family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IFFP Eukaryota
ENOG4111IU4 LUCA
GeneTreeiENSGT00390000010470
HOGENOMiHOG000006539
HOVERGENiHBG039612
InParanoidiA6ND01
KOiK13649
OMAiGRCLQKW
OrthoDBiEOG091G0GIA
PhylomeDBiA6ND01
TreeFamiTF328532

Family and domain databases

InterProiView protein in InterPro
IPR004269 Folate_rcpt
IPR018143 Folate_rcpt-like
PANTHERiPTHR10517 PTHR10517, 1 hit
PfamiView protein in Pfam
PF03024 Folate_rec, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.iShow all

Isoform 1 (identifier: A6ND01-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MACWWPLLLE LWTVMPTWAG DELLNICMNA KHHKRVPSPE DKLYEECIPW
60 70 80 90 100
KDNACCTLTT SWEAHLDVSP LYNFSLFHCG LLMPGCRKHF IQAICFYECS
110 120 130 140 150
PNLGPWIQPV GSLGWEVAPS GQGERVVNVP LCQEDCEEWW EDCRMSYTCK
160 170 180 190 200
SNWRGGWDWS QGKNRCPKGA QCLPFSHYFP TPADLCEKTW SNSFKASPER
210 220 230 240 250
RNSGRCLQKW FEPAQGNPNV AVARLFASSA PSWELSYTIM VCSLFLPFLS
Note: No experimental confirmation available. Gene prediction based on EST data.
Length:250
Mass (Da):28,672
Last modified:January 11, 2011 - v3
Checksum:iCBF2FD967ABAF6E7
GO
Isoform 2 (identifier: A6ND01-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     110-116: Missing.

Note: No experimental confirmation available. Gene prediction based on similarity to orthologs.
Show »
Length:243
Mass (Da):27,943
Checksum:i814B8F7C842BAFBF
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
J3KNP7J3KNP7_HUMAN
Sperm-egg fusion protein Juno
IZUMO1R
250Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_040470110 – 116Missing in isoform 2. Curated7

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP002784 Genomic DNA No translation available.
CCDSiCCDS81615.1 [A6ND01-1]
RefSeqiNP_001186135.1, NM_001199206.1 [A6ND01-1]
UniGeneiHs.553758

Genome annotation databases

EnsembliENST00000328458; ENSP00000332963; ENSG00000183560 [A6ND01-1]
ENST00000440961; ENSP00000416935; ENSG00000183560 [A6ND01-2]
GeneIDi390243
KEGGihsa:390243
UCSCiuc058gou.1 human [A6ND01-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiJUNO_HUMAN
AccessioniPrimary (citable) accession number: A6ND01
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: January 11, 2011
Last modified: September 12, 2018
This is version 79 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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