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Protein

Endochitinase 46

Gene

chit46

Organism
Trichoderma harzianum (Hypocrea lixii)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Secreted chitinase involved in the degradation of chitin, a component of the cell walls of fungi and exoskeletal elements of some animals (including worms and arthropods). Plays a morphogenetic role during apical growth, cell division and differentiation (cell wall morphogenesis). Acts also as an antifungal agent. Involved in the degradation and further assimilation of phytopathogenic fungi, namely mycoparasitism, the major mechanism accounting for the antagonistic activity against phytopathogenic fungi displayed by Trichoderma.1 Publication

Catalytic activityi

Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei172Proton donorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism, Chitin degradation, Polysaccharide degradation
LigandChitin-binding

Protein family/group databases

CAZyiGH18 Glycoside Hydrolase Family 18

Names & Taxonomyi

Protein namesi
Recommended name:
Endochitinase 46 (EC:3.2.1.14)
Alternative name(s):
46 kDa endochitinase
Chitinase 46
Gene namesi
Name:chit46
OrganismiTrichoderma harzianum (Hypocrea lixii)
Taxonomic identifieri5544 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesHypocreomycetidaeHypocrealesHypocreaceaeTrichoderma

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Biotechnological usei

The antagonistic activity of Trichoderma harzianum is used for the control of several soil borne plant pathogenic fungi.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
PropeptideiPRO_000042989223 – 35By similarityAdd BLAST13
ChainiPRO_000042989336 – 430Endochitinase 46Add BLAST395

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi219N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Cleavage on pair of basic residues, Glycoprotein, Zymogen

Structurei

3D structure databases

ProteinModelPortaliA6N6J0
SMRiA6N6J0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi310 – 317Poly-Gly8

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.10.50.10, 1 hit
InterProiView protein in InterPro
IPR011583 Chitinase_II
IPR029070 Chitinase_insertion_sf
IPR001223 Glyco_hydro18_cat
IPR001579 Glyco_hydro_18_chit_AS
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF00704 Glyco_hydro_18, 1 hit
SMARTiView protein in SMART
SM00636 Glyco_18, 1 hit
SUPFAMiSSF51445 SSF51445, 2 hits
SSF54556 SSF54556, 1 hit
PROSITEiView protein in PROSITE
PS01095 CHITINASE_18, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A6N6J0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSFLGKSVA LLAALQATLS SASPLATEER SIEKRANGYA NSVYFTNWGI
60 70 80 90 100
YDRNFQPADL VASDVTHVIY SFMNLQADGT VVSGDTYADF EKHYADDSWN
110 120 130 140 150
DVGTNAYGCA KQLFKVKKAN RGLKVLLSIG GWTWSTNFPS AASTDANRKN
160 170 180 190 200
FAKTAITFMK DWGFDGIDVD WEYPADATQA SNMVLLLKEV RSQLDAYAAQ
210 220 230 240 250
YAPGYHFLLT IAAPAGKDNY SKLRLADLGQ VLDYINLMAY DYAGSFSPLT
260 270 280 290 300
GHDANLFANP SNPNATPFNT DSAVKDYIKG GVPANKIVLG MPIYGRSFQN
310 320 330 340 350
TAGIGQTYNG VGGGGGGSTG SWEAGIWDYK ALPKAGATIQ YDSVAKGYYS
360 370 380 390 400
YNAGTKELIS FDTPDMINTK VAYLKSLGLG GSMFWEASAD KKGADSLIGT
410 420 430
SHRALGGLDT TQNLLSYPNS KYDNIRNGLN
Length:430
Mass (Da):46,380
Last modified:July 24, 2007 - v1
Checksum:iCB09A51FBACFB31B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF613224 Genomic DNA Translation: ABR21205.1

Similar proteinsi

Entry informationi

Entry nameiCHI46_TRIHA
AccessioniPrimary (citable) accession number: A6N6J0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 9, 2014
Last sequence update: July 24, 2007
Last modified: November 22, 2017
This is version 34 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

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