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Protein

Thiamine thiazole synthase

Gene

thi4

Organism
Thermosipho melanesiensis (strain DSM 12029 / CIP 104789 / BI429)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the biosynthesis of the thiazole moiety of thiamine. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylate (ADT), an adenylated thiazole intermediate, using free sulfide as a source of sulfur.UniRule annotation

Cofactori

Fe2+UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei34NADUniRule annotation1
Binding sitei61NAD; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei124NAD; via amide nitrogen and carbonyl oxygenUniRule annotation1
Metal bindingi152Iron; shared with adjacent protomerUniRule annotation1
Metal bindingi167IronUniRule annotation1
Binding sitei170NAD; via carbonyl oxygenUniRule annotation1
Binding sitei219NAD; via amide nitrogenUniRule annotation1
Binding sitei229GlycineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi53 – 54NADUniRule annotation2
Nucleotide bindingi150 – 152NAD; shared with adjacent protomerUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyaseUniRule annotation
Biological processThiamine biosynthesisUniRule annotation
LigandIronUniRule annotation, Metal-bindingUniRule annotation, NADUniRule annotation

Enzyme and pathway databases

BioCyciTMEL391009:G1G91-1649-MONOMER
UniPathwayiUPA00060

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine thiazole synthaseUniRule annotation (EC:4.-.-.-UniRule annotation)
Gene namesi
Name:thi4UniRule annotation
Ordered Locus Names:Tmel_1590Imported
OrganismiThermosipho melanesiensis (strain DSM 12029 / CIP 104789 / BI429)Imported
Taxonomic identifieri391009 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesFervidobacteriaceaeThermosipho
Proteomesi
  • UP000001110 Componenti: Chromosome

Interactioni

Subunit structurei

Homooctamer; tetramer of dimers.UniRule annotation

Protein-protein interaction databases

STRINGi391009.Tmel_1590

Structurei

3D structure databases

ProteinModelPortaliA6LND3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the THI4 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105IQJ Bacteria
COG1635 LUCA
HOGENOMiHOG000106048
KOiK22699
OMAiMWGGGMM
OrthoDBiPOG091H09WG

Family and domain databases

Gene3Di3.50.50.60, 1 hit
HAMAPiMF_00304 Thi4, 1 hit
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR002922 Thi4_fam
IPR022828 Thi4_prok
SUPFAMiSSF51905 SSF51905, 1 hit
TIGRFAMsiTIGR00292 TIGR00292, 1 hit

Sequencei

Sequence statusi: Complete.

A6LND3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWDLEISKII VNGFFEKFND ALDVDVAIVG GGPSALTASY FLTKNGFKVV
60 70 80 90 100
IFEEKNDPGG GTWGGGMLFN ELVVEEELEW MLKEFGMNYK RLNGFISIDS
110 120 130 140 150
VHFASSLLYN TTKVGTKIFN NVIVEDILME ENRLCGVVIN WAPVIKQRLH
160 170 180 190 200
VDPITVKAKY VVDGTGHPAS VVQMIIDRNL EVELPLDKIR EFPMNAKEGE
210 220 230 240 250
NFVLKNTKEV FPGLFVMGMA AVSVGGGPRM GPIFGGMLKS GEKVANAIVE

KLSVEVSK
Length:258
Mass (Da):28,408
Last modified:July 24, 2007 - v1
Checksum:iE8408D49A2BF1DA9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000716 Genomic DNA Translation: ABR31434.1
RefSeqiWP_012057793.1, NC_009616.1

Genome annotation databases

EnsemblBacteriaiABR31434; ABR31434; Tmel_1590
KEGGitme:Tmel_1590

Similar proteinsi

Entry informationi

Entry nameiA6LND3_THEM4
AccessioniPrimary (citable) accession number: A6LND3
Entry historyiIntegrated into UniProtKB/TrEMBL: July 24, 2007
Last sequence update: July 24, 2007
Last modified: July 18, 2018
This is version 74 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

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