UniProtKB - A6H7I5 (DYN2_BOVIN)
Protein
Dynamin-2
Gene
DNM2
Organism
Bos taurus (Bovine)
Status
Functioni
Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Plays a role in the regulation of neuron morphology, axon growth and formation of neuronal growth cones (By similarity). Plays an important role in vesicular trafficking processes, in particular endocytosis. Involved in cytokinesis. Regulates maturation of apoptotic cell corpse-containing phagosomes by recruiting PIK3C3 to the phagosome membrane.By similarity
Catalytic activityi
- EC:3.6.5.5
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 38 – 46 | GTPBy similarity | 9 | |
Nucleotide bindingi | 205 – 211 | GTPBy similarity | 7 | |
Nucleotide bindingi | 236 – 239 | GTPBy similarity | 4 |
GO - Molecular functioni
- GTPase activity Source: GO_Central
- GTP binding Source: UniProtKB-KW
- microtubule binding Source: GO_Central
GO - Biological processi
- dynamin family protein polymerization involved in mitochondrial fission Source: GO_Central
- membrane fusion Source: GO_Central
- mitochondrial fission Source: GO_Central
- neuron projection morphogenesis Source: UniProtKB
- phagocytosis Source: UniProtKB-KW
- postsynaptic neurotransmitter receptor internalization Source: GO_Central
- receptor internalization Source: GO_Central
- receptor-mediated endocytosis Source: UniProtKB
- regulation of axon extension Source: UniProtKB
- regulation of synapse structure or activity Source: GO_Central
- synaptic vesicle budding from presynaptic endocytic zone membrane Source: GO_Central
Keywordsi
Molecular function | Hydrolase, Motor protein |
Biological process | Endocytosis, Phagocytosis |
Ligand | GTP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 3.6.5.5, 908 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:DNM2 |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Cytoskeleton
- cytoskeleton By similarity
Other locations
- Cell junction By similarity
- clathrin-coated pit By similarity
- postsynaptic density By similarity
- synapse By similarity
- Midbody By similarity
- phagocytic cup By similarity
- phagosome membrane By similarity; Peripheral membrane protein By similarity
Note: Colocalizes with CTTN at the basis of filopodia in hippocampus neuron growth zones. Microtubule-associated. Also found in the postsynaptic density of neuronal cells. Co-localizes with PIK3C3 and RAB5A to the nascent phagosome.By similarity
Cytoskeleton
- microtubule Source: UniProtKB-KW
- microtubule cytoskeleton Source: GO_Central
Mitochondrion
- mitochondrial membrane Source: GO_Central
Plasma Membrane
- clathrin-coated pit Source: UniProtKB
- phagocytic cup Source: UniProtKB-SubCell
- plasma membrane Source: GO_Central
- postsynaptic endocytic zone membrane Source: GO_Central
Other locations
- axon Source: GO_Central
- cell junction Source: UniProtKB
- cytoplasm Source: GO_Central
- cytoplasmic vesicle Source: GO_Central
- dendritic spine head Source: GO_Central
- membrane Source: GO_Central
- midbody Source: UniProtKB-SubCell
- phagocytic vesicle membrane Source: UniProtKB-SubCell
- postsynaptic density Source: GO_Central
- presynapse Source: GOC
Keywords - Cellular componenti
Cell junction, Cell projection, Coated pit, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Membrane, Microtubule, SynapsePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000319950 | 1 – 866 | Dynamin-2Add BLAST | 866 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 231 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 299 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 593 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 594 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 751 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 760 | Phosphoserine; by CDK1By similarity | 1 |
Post-translational modificationi
Phosphorylation at Ser-760 by CDK1 is greatly increased upon mitotic entry. It regulates cytokinesis downstream of calcineurin, and does not affect clathrin-mediated endocytosis. Dephosphorylated by calcineurin/PP2 (By similarity). Phosphorylated on tyrosine residues after activation of SRC (By similarity).By similarity
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
PaxDbi | A6H7I5 |
PRIDEi | A6H7I5 |
Interactioni
Subunit structurei
Interacts with MYOF.
Interacts with CTTN and ACTN1.
Interacts with SHANK1, SHANK2, SH3BP4 and NOSTRIN.
Interacts with SNX9.
Interacts with SNX33 (via SH3 domain).
Interacts with MYO1E (via SH3 domain).
Interacts with PSTPIP1.
Interacts with CTNND2. May interact with PIK3C3. May be a component of a complex composed of RAB5A (in GDP-bound form), DYN2 and PIK3C3.
Interacts with BIN1 (By similarity).
By similarityGO - Molecular functioni
- microtubule binding Source: GO_Central
Protein-protein interaction databases
STRINGi | 9913.ENSBTAP00000035986 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 28 – 294 | Dynamin-type GPROSITE-ProRule annotationAdd BLAST | 267 | |
Domaini | 515 – 621 | PHPROSITE-ProRule annotationAdd BLAST | 107 | |
Domaini | 649 – 740 | GEDPROSITE-ProRule annotationAdd BLAST | 92 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 38 – 45 | G1 motifPROSITE-ProRule annotation | 8 | |
Regioni | 64 – 66 | G2 motifPROSITE-ProRule annotation | 3 | |
Regioni | 136 – 139 | G3 motifPROSITE-ProRule annotation | 4 | |
Regioni | 205 – 208 | G4 motifPROSITE-ProRule annotation | 4 | |
Regioni | 235 – 238 | G5 motifPROSITE-ProRule annotation | 4 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 743 – 862 | Pro-richAdd BLAST | 120 |
Sequence similaritiesi
Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family.PROSITE-ProRule annotation
Phylogenomic databases
eggNOGi | KOG0446, Eukaryota |
InParanoidi | A6H7I5 |
OrthoDBi | 264244at2759 |
Family and domain databases
CDDi | cd08771, DLP_1, 1 hit |
Gene3Di | 2.30.29.30, 1 hit |
InterProi | View protein in InterPro IPR027188, DNM2 IPR001401, Dynamin_GTPase IPR019762, Dynamin_GTPase_CS IPR022812, Dynamin_SF IPR000375, Dynamin_stalk IPR030381, G_DYNAMIN_dom IPR003130, GED IPR020850, GED_dom IPR027417, P-loop_NTPase IPR011993, PH-like_dom_sf IPR001849, PH_domain |
PANTHERi | PTHR11566, PTHR11566, 1 hit PTHR11566:SF23, PTHR11566:SF23, 1 hit |
Pfami | View protein in Pfam PF01031, Dynamin_M, 1 hit PF00350, Dynamin_N, 1 hit PF02212, GED, 1 hit PF00169, PH, 1 hit |
PRINTSi | PR00195, DYNAMIN |
SMARTi | View protein in SMART SM00053, DYNc, 1 hit SM00302, GED, 1 hit SM00233, PH, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00410, G_DYNAMIN_1, 1 hit PS51718, G_DYNAMIN_2, 1 hit PS51388, GED, 1 hit PS50003, PH_DOMAIN, 1 hit |
i Sequence
Sequence statusi: Complete.
A6H7I5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGNRGMEELI PLVNKLQDAF SSIGQSCHLD LPQIAVVGGQ SAGKSSVLEN
60 70 80 90 100
FVGRDFLPRG SGIVTRRPLI LQLIFSKTEY AEFLHCKSRK FTDFEEVRQE
110 120 130 140 150
IEAETDRVTG TNKGISPVPI NLRIYSPHVL NLTLIDLPGI TKVPVGDQPQ
160 170 180 190 200
DIEYQIKDMI LQFISRESSL ILAVTPANMD LANSDALKLA KEVDPQGLRT
210 220 230 240 250
IGVITKLDLM DEGTDARDVL ENKLLPLRRG YIGVVNRSQK DIEGKKDIRT
260 270 280 290 300
ALAAERKFFL SHPAYRHIAD RMGTPHLQKT LNQQLTNHIR ESLPALRSKL
310 320 330 340 350
QSQLLSLEKE VEEYKNFRPD DPTRKTKALL QMVQQFGVDF EKRIEGSGDQ
360 370 380 390 400
VDTLELSGGA RINRIFHERF PFELVKMEFD EKDLRREISY AIKNIHGVRT
410 420 430 440 450
GLFTPDLAFE AIVKKQVVKL KEPCLKCVDL VIQELINTVR QCTSKLSSYP
460 470 480 490 500
RLREETERIV TTYIREREGR TKDQILLLID IEQSYINTNH EDFIGFANAQ
510 520 530 540 550
QRSTQLNKKR AVPNQVIRRG WLTINNISLM KGGSKEYWFV LTAESLSWYK
560 570 580 590 600
DEEEKEKKYM LPLDNLKIRD VEKGFMSNKH VFAIFNTEQR NVYKDLRQIE
610 620 630 640 650
LACDSQEDVD SWKASFLRAG VYPEKDQAEN EDGAQENTFS MDPQLERQVE
660 670 680 690 700
TIRNLVDSYV AIINKSIRDL MPKTIMHLMI NNTKAFIHYE LLAYLYSSAD
710 720 730 740 750
QSSLMEESAD QAQRRDDMLR MYHALKEALN IIGDISTSTV STPVPPPVDD
760 770 780 790 800
TWIQNTSSHS PTPQRRPVSS VHPPGRPPAV RGPTPGPPLI PVPVGPASFS
810 820 830 840 850
APPIPSRPGP HPGVFANNDP FSAPPQIPSR PARIPPGIPP GVPSRRPPAA
860
PSRPTIIRPA EPSLLD
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC146259 mRNA Translation: AAI46260.1 |
RefSeqi | NP_001092839.1, NM_001099369.1 |
Genome annotation databases
GeneIDi | 511691 |
KEGGi | bta:511691 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC146259 mRNA Translation: AAI46260.1 |
RefSeqi | NP_001092839.1, NM_001099369.1 |
3D structure databases
BMRBi | A6H7I5 |
SMRi | A6H7I5 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9913.ENSBTAP00000035986 |
Proteomic databases
PaxDbi | A6H7I5 |
PRIDEi | A6H7I5 |
Genome annotation databases
GeneIDi | 511691 |
KEGGi | bta:511691 |
Organism-specific databases
CTDi | 1785 |
Phylogenomic databases
eggNOGi | KOG0446, Eukaryota |
InParanoidi | A6H7I5 |
OrthoDBi | 264244at2759 |
Enzyme and pathway databases
BRENDAi | 3.6.5.5, 908 |
Family and domain databases
CDDi | cd08771, DLP_1, 1 hit |
Gene3Di | 2.30.29.30, 1 hit |
InterProi | View protein in InterPro IPR027188, DNM2 IPR001401, Dynamin_GTPase IPR019762, Dynamin_GTPase_CS IPR022812, Dynamin_SF IPR000375, Dynamin_stalk IPR030381, G_DYNAMIN_dom IPR003130, GED IPR020850, GED_dom IPR027417, P-loop_NTPase IPR011993, PH-like_dom_sf IPR001849, PH_domain |
PANTHERi | PTHR11566, PTHR11566, 1 hit PTHR11566:SF23, PTHR11566:SF23, 1 hit |
Pfami | View protein in Pfam PF01031, Dynamin_M, 1 hit PF00350, Dynamin_N, 1 hit PF02212, GED, 1 hit PF00169, PH, 1 hit |
PRINTSi | PR00195, DYNAMIN |
SMARTi | View protein in SMART SM00053, DYNc, 1 hit SM00302, GED, 1 hit SM00233, PH, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00410, G_DYNAMIN_1, 1 hit PS51718, G_DYNAMIN_2, 1 hit PS51388, GED, 1 hit PS50003, PH_DOMAIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DYN2_BOVIN | |
Accessioni | A6H7I5Primary (citable) accession number: A6H7I5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 26, 2008 |
Last sequence update: | July 24, 2007 | |
Last modified: | April 7, 2021 | |
This is version 99 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families