UniProtKB - A6H6E2 (MMRN2_MOUSE)
Protein
Multimerin-2
Gene
Mmrn2
Organism
Mus musculus (Mouse)
Status
Functioni
Inhibits endothelial cells motility and acts as a negative regulator of angiogenesis; it downregulates KDR activation by binding VEGFA.By similarity
GO - Biological processi
- cell migration involved in sprouting angiogenesis Source: MGI
- negative regulation of cell migration involved in sprouting angiogenesis Source: MGI
- negative regulation of vascular endothelial growth factor receptor signaling pathway Source: MGI
Keywordsi
Biological process | Angiogenesis |
Names & Taxonomyi
Protein namesi | Recommended name: Multimerin-2Imported |
Gene namesi | Name:Mmrn2Imported |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2385618, Mmrn2 |
Subcellular locationi
Extracellular region or secreted
- extracellular matrix By similarity
Extracellular region or secreted
- basement membrane Source: MGI
- collagen-containing extracellular matrix Source: BHF-UCL
- extracellular matrix Source: MGI
- extracellular space Source: UniProtKB
Keywords - Cellular componenti
Extracellular matrix, SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 22 | Sequence analysisAdd BLAST | 22 | |
ChainiPRO_0000367040 | 23 – 943 | Multimerin-2Sequence analysisAdd BLAST | 921 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 58 ↔ 122 | PROSITE-ProRule annotation | ||
Disulfide bondi | 85 ↔ 92 | PROSITE-ProRule annotation | ||
Disulfide bondi | 121 ↔ 130 | PROSITE-ProRule annotation | ||
Glycosylationi | 205 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 214 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 249 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 261 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 350 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 379 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 439 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 472 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 583 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 728 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 766 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 839 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Post-translational modificationi
N- and O-glycosylated.By similarity
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
CPTACi | non-CPTAC-3482 |
MaxQBi | A6H6E2 |
PaxDbi | A6H6E2 |
PeptideAtlasi | A6H6E2 |
PRIDEi | A6H6E2 |
PTM databases
GlyConnecti | 2515, 1 N-Linked glycan (1 site) |
GlyGeni | A6H6E2, 12 sites |
PhosphoSitePlusi | A6H6E2 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000041445, Expressed in brain blood vessel and 234 other tissues |
ExpressionAtlasi | A6H6E2, baseline and differential |
Genevisiblei | A6H6E2, MM |
Interactioni
Subunit structurei
Heteromer of p110, p125, p140 and p200 subunits; disulfide-linked.
Interacts with VEGFA.
By similarityProtein-protein interaction databases
BioGRIDi | 222862, 4 interactors |
ComplexPortali | CPX-451, Multimerin-2 complex |
STRINGi | 10090.ENSMUSP00000107539 |
Miscellaneous databases
RNActi | A6H6E2, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 54 – 132 | EMIPROSITE-ProRule annotationAdd BLAST | 79 | |
Domaini | 815 – 943 | C1qPROSITE-ProRule annotationAdd BLAST | 129 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 391 – 480 | Sequence analysisAdd BLAST | 90 | |
Coiled coili | 551 – 580 | Sequence analysisAdd BLAST | 30 | |
Coiled coili | 688 – 720 | Sequence analysisAdd BLAST | 33 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 356 – 362 | Poly-AlaSequence analysis | 7 |
Keywords - Domaini
Coiled coil, SignalPhylogenomic databases
eggNOGi | ENOG502QUTH, Eukaryota |
GeneTreei | ENSGT01010000222524 |
HOGENOMi | CLU_012255_1_0_1 |
InParanoidi | A6H6E2 |
OMAi | ANQTGHE |
OrthoDBi | 1205089at2759 |
PhylomeDBi | A6H6E2 |
TreeFami | TF336041 |
Family and domain databases
Gene3Di | 2.60.120.40, 1 hit |
InterProi | View protein in InterPro IPR001073, C1q_dom IPR011489, EMI_domain IPR008983, Tumour_necrosis_fac-like_dom |
Pfami | View protein in Pfam PF00386, C1q, 1 hit PF07546, EMI, 1 hit |
SMARTi | View protein in SMART SM00110, C1Q, 1 hit |
SUPFAMi | SSF49842, SSF49842, 1 hit |
PROSITEi | View protein in PROSITE PS50871, C1Q, 1 hit PS51041, EMI, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 12 Publications (identifier: A6H6E2-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MIPTLLLGFG VYLSWGLLGS WAQDPGTKFS HLNRPGMPEG WRLGAEDTSR
60 70 80 90 100
DPIRRNWCPY QKSRLVTFVA ACKTEKFLVH SQQPCPQGAP DCQGVRVMYR
110 120 130 140 150
VAQKPVYQVQ QKVLISVDWR CCPGFQGPDC QDHNPTANPE PTEPSGKLQE
160 170 180 190 200
TWDSMDGFEL GHPVPEFNEI KVPQEQQENL LQNLQNDAQS VEDGFPGSWE
210 220 230 240 250
APPSNLTDEM TEANLTEFEF PGRTSEHLLQ PHIDAFLKAH FSPIWKNFND
260 270 280 290 300
SLHSLSQAIR NLSLDVEANH QAIKMIQEGT VARADFQELG AKFEAKVQQN
310 320 330 340 350
SQRLGQLWQD VEDQLHAQRR SVHHALSDVQ AEVSTKLKQL VKAQELPGAN
360 370 380 390 400
GSLVMASAAA AARPEPESLQ ARLGQLQRNL SALHMVTSQR EEELQSTLKN
410 420 430 440 450
MDSVLKQHAE EIKELYSESD ETFDQISKVE RQVEELLVNH TGLRELRVIL
460 470 480 490 500
MEKSLIMEEN KEEIERQLLE LNLTLQHLHA GHADLIKYVK DCNCQRVNSD
510 520 530 540 550
VDVAPEGHRD VMHTLEETQV SLDEQHQLDG SSLQALQSTV DAMSSAMDAY
560 570 580 590 600
RGEGERARAE RARIRSQLRA LDHAVEALKT AANGTRKEIR LLHGSFTALL
610 620 630 640 650
EDALRHQAVL AALFGEEMID EMSEEAPRPL PLDYEQIRLA LQDAASGLQE
660 670 680 690 700
QAIGWEDLAT RVEALEKAAG GFVEQHPQLA EGLEPSHDSG REEEAMTLAE
710 720 730 740 750
LEQEIRRLSS DVKQIGQCCE ASWAASLNSS LEDLHSMLLD TQHGLRQHRQ
760 770 780 790 800
LFHNLFQNFQ GLVASNISLD LGKLQAMLSK KDKKQPRGPG ESRKRDKKQV
810 820 830 840 850
VMSTDAHAKG LELWETGSPV AFYAGSSEGA TALQMVKFNT TSINVGSSYF
860 870 880 890 900
PEHGYFRAPK RGVYLFAVSI TFGPGPGMGQ LVFEGHHRVP VYSTEQRGGS
910 920 930 940
TATTFAMVEL QKGERAWFEL IQGSATKGSQ PGTAFGGFLM FKT
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A2I3BPZ4 | A0A2I3BPZ4_MOUSE | Multimerin-2 | Mmrn2 | 173 | Annotation score: |
Sequence cautioni
The sequence BAC37291 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 352 | S → G in BAC37291 (PubMed:16141072).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_053050 | 177 – 702 | Missing in isoform 2. 1 PublicationAdd BLAST | 526 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK078470 mRNA Translation: BAC37291.1 Frameshift. CH466573 Genomic DNA Translation: EDL24879.1 BC034871 mRNA Translation: AAH34871.1 BC145845 mRNA Translation: AAI45846.1 |
CCDSi | CCDS36876.1 [A6H6E2-1] |
RefSeqi | NP_694767.3, NM_153127.3 [A6H6E2-1] |
Genome annotation databases
Ensembli | ENSMUST00000111908; ENSMUSP00000107539; ENSMUSG00000041445 [A6H6E2-1] |
GeneIDi | 105450 |
KEGGi | mmu:105450 |
UCSCi | uc007tav.1, mouse [A6H6E2-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK078470 mRNA Translation: BAC37291.1 Frameshift. CH466573 Genomic DNA Translation: EDL24879.1 BC034871 mRNA Translation: AAH34871.1 BC145845 mRNA Translation: AAI45846.1 |
CCDSi | CCDS36876.1 [A6H6E2-1] |
RefSeqi | NP_694767.3, NM_153127.3 [A6H6E2-1] |
3D structure databases
SMRi | A6H6E2 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 222862, 4 interactors |
ComplexPortali | CPX-451, Multimerin-2 complex |
STRINGi | 10090.ENSMUSP00000107539 |
PTM databases
GlyConnecti | 2515, 1 N-Linked glycan (1 site) |
GlyGeni | A6H6E2, 12 sites |
PhosphoSitePlusi | A6H6E2 |
Proteomic databases
CPTACi | non-CPTAC-3482 |
MaxQBi | A6H6E2 |
PaxDbi | A6H6E2 |
PeptideAtlasi | A6H6E2 |
PRIDEi | A6H6E2 |
Protocols and materials databases
Antibodypediai | 16004, 100 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000111908; ENSMUSP00000107539; ENSMUSG00000041445 [A6H6E2-1] |
GeneIDi | 105450 |
KEGGi | mmu:105450 |
UCSCi | uc007tav.1, mouse [A6H6E2-1] |
Organism-specific databases
CTDi | 79812 |
MGIi | MGI:2385618, Mmrn2 |
Phylogenomic databases
eggNOGi | ENOG502QUTH, Eukaryota |
GeneTreei | ENSGT01010000222524 |
HOGENOMi | CLU_012255_1_0_1 |
InParanoidi | A6H6E2 |
OMAi | ANQTGHE |
OrthoDBi | 1205089at2759 |
PhylomeDBi | A6H6E2 |
TreeFami | TF336041 |
Miscellaneous databases
BioGRID-ORCSi | 105450, 2 hits in 17 CRISPR screens |
ChiTaRSi | Mmrn2, mouse |
PROi | PR:A6H6E2 |
RNActi | A6H6E2, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000041445, Expressed in brain blood vessel and 234 other tissues |
ExpressionAtlasi | A6H6E2, baseline and differential |
Genevisiblei | A6H6E2, MM |
Family and domain databases
Gene3Di | 2.60.120.40, 1 hit |
InterProi | View protein in InterPro IPR001073, C1q_dom IPR011489, EMI_domain IPR008983, Tumour_necrosis_fac-like_dom |
Pfami | View protein in Pfam PF00386, C1q, 1 hit PF07546, EMI, 1 hit |
SMARTi | View protein in SMART SM00110, C1Q, 1 hit |
SUPFAMi | SSF49842, SSF49842, 1 hit |
PROSITEi | View protein in PROSITE PS50871, C1Q, 1 hit PS51041, EMI, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MMRN2_MOUSE | |
Accessioni | A6H6E2Primary (citable) accession number: A6H6E2 Secondary accession number(s): Q8BJX4, Q8K1Z7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 24, 2009 |
Last sequence update: | July 24, 2007 | |
Last modified: | December 2, 2020 | |
This is version 95 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot