UniProtKB - A5WVX8 (A5WVX8_DANRE)
Protein
Methionine aminopeptidase 2
Gene
metap2a
Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Functioni
Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).UniRule annotation
Protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. Plays a critical role in the regulation of protein synthesis.UniRule annotation
Catalytic activityi
- Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.UniRule annotationARBA annotation EC:3.4.11.18
Cofactori
Co2+UniRule annotation, Zn2+UniRule annotation, Mn2+UniRule annotation, Fe2+UniRule annotationNote: Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe2+-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 221 | SubstrateUniRule annotation | 1 | |
Metal bindingi | 241 | Divalent metal cation 1UniRule annotation | 1 | |
Metal bindingi | 252 | Divalent metal cation 1UniRule annotation | 1 | |
Metal bindingi | 252 | Divalent metal cation 2; catalyticUniRule annotation | 1 | |
Metal bindingi | 321 | Divalent metal cation 2; catalytic; via tele nitrogenUniRule annotation | 1 | |
Binding sitei | 329 | SubstrateUniRule annotation | 1 | |
Metal bindingi | 354 | Divalent metal cation 2; catalyticUniRule annotation | 1 | |
Metal bindingi | 449 | Divalent metal cation 1UniRule annotation | 1 | |
Metal bindingi | 449 | Divalent metal cation 2; catalyticUniRule annotation | 1 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-UniRule
- metalloaminopeptidase activity Source: UniProtKB-UniRule
GO - Biological processi
- protein initiator methionine removal Source: UniProtKB-UniRule
Keywordsi
Molecular function | AminopeptidaseUniRule annotationARBA annotation, Hydrolase, Protease |
Ligand | Metal-bindingUniRule annotationARBA annotation |
Protein family/group databases
MEROPSi | M24.002 |
Names & Taxonomyi
Protein namesi | Recommended name: Methionine aminopeptidase 2UniRule annotation (EC:3.4.11.18UniRule annotation)Short name: MAP 2UniRule annotation Short name: MetAP 2UniRule annotation Alternative name(s): Initiation factor 2-associated 67 kDa glycoproteinUniRule annotation Peptidase MUniRule annotation Short name: p67UniRule annotation Short name: p67eIF2UniRule annotation |
Gene namesi | |
Organismi | Danio rerio (Zebrafish) (Brachydanio rerio)Imported |
Taxonomic identifieri | 7955 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Danionidae › Danioninae › Danio |
Proteomesi |
|
Organism-specific databases
ZFINi | ZDB-GENE-061013-5, metap2a |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation
Note: About 30% of expressed METAP2 associates with polysomes.UniRule annotation
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmUniRule annotationPTM / Processingi
Post-translational modificationi
Contains approximately 12 O-linked N-acetylglucosamine (GlcNAc) residues. O-glycosylation is required for EIF2S1 binding.UniRule annotation
Keywords - PTMi
GlycoproteinUniRule annotationProteomic databases
PaxDbi | A5WVX8 |
PeptideAtlasi | A5WVX8 |
Expressioni
Gene expression databases
Bgeei | ENSDARG00000069261, Expressed in multi-cellular organism and 25 other tissues |
Interactioni
Subunit structurei
Binds EIF2S1 at low magnesium concentrations.
Interacts strongly with the eIF-2 gamma-subunit EIF2S3.
UniRule annotationProtein-protein interaction databases
STRINGi | 7955.ENSDARP00000109712 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 158 – 455 | Peptidase_M24InterPro annotationAdd BLAST | 298 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 109 | DisorderedSequence analysisAdd BLAST | 109 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 48 – 67 | PolyampholyteSequence analysisAdd BLAST | 20 |
Sequence similaritiesi
Belongs to the peptidase M24 family.ARBA annotation
Belongs to the peptidase M24A family. Methionine aminopeptidase eukaryotic type 2 subfamily.UniRule annotation
Phylogenomic databases
eggNOGi | KOG2775, Eukaryota |
GeneTreei | ENSGT00940000167341 |
HOGENOMi | CLU_015857_7_2_1 |
InParanoidi | A5WVX8 |
OMAi | ALCIGQN |
OrthoDBi | 601484at2759 |
PhylomeDBi | A5WVX8 |
TreeFami | TF300426 |
Family and domain databases
CDDi | cd01088, MetAP2, 1 hit |
Gene3Di | 1.10.10.10, 1 hit 3.90.230.10, 1 hit |
HAMAPi | MF_03175, MetAP_2_euk, 1 hit |
InterProi | View protein in InterPro IPR036005, Creatinase/aminopeptidase-like IPR000994, Pept_M24 IPR001714, Pept_M24_MAP IPR002468, Pept_M24A_MAP2 IPR018349, Pept_M24A_MAP2_BS IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
Pfami | View protein in Pfam PF00557, Peptidase_M24, 1 hit |
PRINTSi | PR00599, MAPEPTIDASE |
SUPFAMi | SSF46785, SSF46785, 1 hit SSF55920, SSF55920, 1 hit |
TIGRFAMsi | TIGR00501, met_pdase_II, 1 hit |
PROSITEi | View protein in PROSITE PS01202, MAP_2, 1 hit |
i Sequence
Sequence statusi: Complete.
A5WVX8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEANGLPVPP VMDTLIVNGE REAEDPAKKK KRKKKKNKSV VSAGHSEQVD
60 70 80 90 100
DGELEVKKPL EQQNLEEKER EEDGEEDGEE ADNTSGKKKK KKPKKKKGPK
110 120 130 140 150
TQTDPPSVPI CDLYPSGVFP VGQECEYPPS QDGRSAAWRM SDEEKRVLDE
160 170 180 190 200
ANEEMWSDFR QAAEAHRQVR RYVKSWIKPG MTMIDICERL EACSRRLIKE
210 220 230 240 250
DGLNAGLAFP TGCSLNNCAA HYTPNAGDPT VLRYDDVCKI DFGTHINGRI
260 270 280 290 300
IDCAFTVTFN PKFDHLLEAV ADATNTGIKC AGIDVRLCDV GESIQEVMES
310 320 330 340 350
YEVEIDGKTY QVKPIRNLNG HSIGQYRIHA GKTVPIVKGG EATRMEEGEV
360 370 380 390 400
YAIETFGSTG KGVVHDDMDC SHYMKNFDVG HVPIRLPRAK HLLNVVNENF
410 420 430 440 450
GTLAFCRRWL DRLGESKYLM ALKNLCDLGI IDPYPPLCDT KGSYTAQFEH
460
TILLRPTCKE VVSRGDDY
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CT573048 Genomic DNA No translation available. |
RefSeqi | NP_001093524.1, NM_001100054.1 |
Genome annotation databases
Ensembli | ENSDART00000125767; ENSDARP00000109712; ENSDARG00000069261 |
GeneIDi | 794589 |
KEGGi | dre:794589 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CT573048 Genomic DNA No translation available. |
RefSeqi | NP_001093524.1, NM_001100054.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 7955.ENSDARP00000109712 |
Protein family/group databases
MEROPSi | M24.002 |
Proteomic databases
PaxDbi | A5WVX8 |
PeptideAtlasi | A5WVX8 |
Genome annotation databases
Ensembli | ENSDART00000125767; ENSDARP00000109712; ENSDARG00000069261 |
GeneIDi | 794589 |
KEGGi | dre:794589 |
Organism-specific databases
CTDi | 794589 |
ZFINi | ZDB-GENE-061013-5, metap2a |
Phylogenomic databases
eggNOGi | KOG2775, Eukaryota |
GeneTreei | ENSGT00940000167341 |
HOGENOMi | CLU_015857_7_2_1 |
InParanoidi | A5WVX8 |
OMAi | ALCIGQN |
OrthoDBi | 601484at2759 |
PhylomeDBi | A5WVX8 |
TreeFami | TF300426 |
Gene expression databases
Bgeei | ENSDARG00000069261, Expressed in multi-cellular organism and 25 other tissues |
Family and domain databases
CDDi | cd01088, MetAP2, 1 hit |
Gene3Di | 1.10.10.10, 1 hit 3.90.230.10, 1 hit |
HAMAPi | MF_03175, MetAP_2_euk, 1 hit |
InterProi | View protein in InterPro IPR036005, Creatinase/aminopeptidase-like IPR000994, Pept_M24 IPR001714, Pept_M24_MAP IPR002468, Pept_M24A_MAP2 IPR018349, Pept_M24A_MAP2_BS IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
Pfami | View protein in Pfam PF00557, Peptidase_M24, 1 hit |
PRINTSi | PR00599, MAPEPTIDASE |
SUPFAMi | SSF46785, SSF46785, 1 hit SSF55920, SSF55920, 1 hit |
TIGRFAMsi | TIGR00501, met_pdase_II, 1 hit |
PROSITEi | View protein in PROSITE PS01202, MAP_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | A5WVX8_DANRE | |
Accessioni | A5WVX8Primary (citable) accession number: A5WVX8 | |
Entry historyi | Integrated into UniProtKB/TrEMBL: | July 10, 2007 |
Last sequence update: | July 10, 2007 | |
Last modified: | April 7, 2021 | |
This is version 109 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Unreviewed (UniProtKB/TrEMBL) |