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Entry version 64 (12 Aug 2020)
Sequence version 1 (10 Jul 2007)
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Protein

Carbamoyl-phosphate synthase

Gene

Msm_0361

Organism
Methanobrevibacter smithii (strain ATCC 35061 / DSM 861 / OCM 144 / PS)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes the synthesis of carbamoyl phosphate from ATP, ammonium and bicarbonate. Proceeds via a three-step mechanism, i.e. the phosphorylation of hydrogencarbonate to carboxyphosphate, a nucleophilic attack of ammonia on carboxyphosphate yielding carbamate, and the phosphorylation of carbamate forming carbamoyl phosphate. In M.smithii, the predominant archaeon in the human gut, one function of this enzyme may be to sequester ammonia, a scarce nutrient in the intestine which is the major source of nitrogen in M.smithii for the biosynthesis of nucleotides, amino acids, and many other metabolites.1 Publication

Miscellaneous

Several lines of evidence suggest that obesity is associated with elevated levels of intestinal methanogenic archaea. Consequently, this enzyme may be an attractive drug target in the treatment of human obesity.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Mg2+PROSITE-ProRule annotation, Mn2+PROSITE-ProRule annotationNote: Binds 2 magnesium or manganese ions per subunit.PROSITE-ProRule annotation

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

kcat is 0.043 sec(-1) towards ATP (at 38 degrees Celsius and pH 8.0). kcat is 0.052 sec(-1) towards NH4+ (at 38 degrees Celsius and pH 8.0). kcat is 0.044 sec(-1) towards hydrogencarbonate (at 38 degrees Celsius and pH 8.0).1 Publication
  1. KM=0.61 mM for ATP (at 38 degrees Celsius and pH 8.0)1 Publication
  2. KM=15.6 mM for NH4+ (at 38 degrees Celsius and pH 8.0)1 Publication
  3. KM=14.5 mM for hydrogencarbonate (at 38 degrees Celsius and pH 8.0)1 Publication
  1. Vmax=62 nmol/min/mg enzyme towards ATP (at 38 degrees Celsius and pH 8.0)1 Publication
  2. Vmax=75 nmol/min/mg enzyme towards NH4+ (at 38 degrees Celsius and pH 8.0)1 Publication
  3. Vmax=64 nmol/min/mg enzyme towards hydrogencarbonate (at 38 degrees Celsius and pH 8.0)1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the 'Description' field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi253Magnesium or manganese 1PROSITE-ProRule annotation1
Metal bindingi267Magnesium or manganese 1PROSITE-ProRule annotation1
Metal bindingi267Magnesium or manganese 2PROSITE-ProRule annotation1
Metal bindingi269Magnesium or manganese 2PROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi137 – 186ATPPROSITE-ProRule annotationAdd BLAST50

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionLigase
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
MSMI420247:GHWZ-366-MONOMER

BRENDA Comprehensive Enzyme Information System

More...
BRENDAi
6.3.4.16, 11234

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Carbamoyl-phosphate synthaseUniRule annotation1 Publication (EC:6.3.4.16UniRule annotation1 Publication)
Alternative name(s):
Carbamoyl phosphate synthetase1 PublicationUniRule annotation
Short name:
CPSase1 PublicationUniRule annotation
SYN31 Publication
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Ordered Locus Names:Msm_0361Imported
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiMethanobrevibacter smithii (strain ATCC 35061 / DSM 861 / OCM 144 / PS)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri420247 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiArchaeaEuryarchaeotaMethanomada groupMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanobrevibacter
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000001992 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00004480041 – 367Carbamoyl-phosphate synthaseAdd BLAST367

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Forms homodimers and homotetramers (dimers of dimers).

1 Publication

Protein-protein interaction databases

STRING: functional protein association networks

More...
STRINGi
420247.Msm_0361

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
A5UK38

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini111 – 296ATP-graspPROSITE-ProRule annotationAdd BLAST186

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

Lacks the domain involved in allosteric regulation present in CarB CPSases.1 Publication

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the small carbamoyl-phosphate synthase family.UniRule annotationCurated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
arCOG01596, Archaea

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_734905_0_0_2

KEGG Orthology (KO)

More...
KOi
K01961

Identification of Orthologs from Complete Genome Data

More...
OMAi
YQRITIR

Family and domain databases

HAMAP database of protein families

More...
HAMAPi
MF_02221, CPSase, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR011761, ATP-grasp
IPR003806, ATP-grasp_PylC-type

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF02655, ATP-grasp_3, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS50975, ATP_GRASP, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

A5UK38-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKILFIGSRL YDDIDYYVRE NGIESIITES NEDAINLDLP DQVFIVPRGM
60 70 80 90 100
DSPKQIAISQ NVDAVVPLIG IDPPLIEVAK MKEELEAETD IPVIAANVRA
110 120 130 140 150
VRLTSDKIKT KEFYNEIGVP TPQYQILAKD DFESKLKMEF PVVLKQGQGQ
160 170 180 190 200
GGKDIKVAES LDDVKEYFEE FDHALCEKFI EGSEISIEVL GYNGEYVPLS
210 220 230 240 250
PIYKGETTLE GIHPLNKIKT APCLVEGLDN NLVQRTAYKV AKNLGSDGIF
260 270 280 290 300
EMDFMFSKDE QQLYAIEVNT RPNGTRYLTT ATCGVNSLCE LVNMAAGKFS
310 320 330 340 350
IKDIQDKLEY YYATEIPIGR YKGPDLNEPL KSFKNNDWVV HGPEGYQRIT
360
IRADSKEQLD RYVEDLI
Length:367
Mass (Da):41,403
Last modified:July 10, 2007 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i088D98457C6E387D
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
CP000678 Genomic DNA Translation: ABQ86566.1

NCBI Reference Sequences

More...
RefSeqi
WP_011953845.1, NC_009515.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
ABQ86566; ABQ86566; Msm_0361

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
5216509

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
msi:Msm_0361

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|420247.28.peg.362

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000678 Genomic DNA Translation: ABQ86566.1
RefSeqiWP_011953845.1, NC_009515.1

3D structure databases

SMRiA5UK38
ModBaseiSearch...

Protein-protein interaction databases

STRINGi420247.Msm_0361

Genome annotation databases

EnsemblBacteriaiABQ86566; ABQ86566; Msm_0361
GeneIDi5216509
KEGGimsi:Msm_0361
PATRICifig|420247.28.peg.362

Phylogenomic databases

eggNOGiarCOG01596, Archaea
HOGENOMiCLU_734905_0_0_2
KOiK01961
OMAiYQRITIR

Enzyme and pathway databases

BioCyciMSMI420247:GHWZ-366-MONOMER
BRENDAi6.3.4.16, 11234

Family and domain databases

HAMAPiMF_02221, CPSase, 1 hit
InterProiView protein in InterPro
IPR011761, ATP-grasp
IPR003806, ATP-grasp_PylC-type
PfamiView protein in Pfam
PF02655, ATP-grasp_3, 1 hit
PROSITEiView protein in PROSITE
PS50975, ATP_GRASP, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiCPS_METS3
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: A5UK38
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 16, 2019
Last sequence update: July 10, 2007
Last modified: August 12, 2020
This is version 64 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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