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Protein

Thymidylate synthase

Gene

thyA

Organism
Haemophilus influenzae (strain PittEE)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei22dUMPUniRule annotation1
Active sitei160NucleophileUniRule annotation1
Binding sitei1835,10-methylenetetrahydrofolateUniRule annotation1
Binding sitei191dUMPUniRule annotation1
Binding sitei2825,10-methylenetetrahydrofolate; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi180 – 183dUMPUniRule annotation4
Nucleotide bindingi221 – 223dUMPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processNucleotide biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00575

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthaseUniRule annotation (EC:2.1.1.45UniRule annotation)
Short name:
TSUniRule annotation
Short name:
TSaseUniRule annotation
Gene namesi
Name:thyAUniRule annotation
Ordered Locus Names:CGSHiEE_07490
OrganismiHaemophilus influenzae (strain PittEE)
Taxonomic identifieri374930 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000006051 – 283Thymidylate synthaseAdd BLAST283

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA5UDH0
SMRiA5UDH0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000257900
KOiK00560
OMAiKQYLDLC

Family and domain databases

CDDicd00351 TS_Pyrimidine_HMase, 1 hit
Gene3Di3.30.572.10, 2 hits
HAMAPiMF_00008 Thymidy_synth_bact, 1 hit
InterProiView protein in InterPro
IPR023451 Thymidate_synth/dCMP_Mease
IPR036926 Thymidate_synth/dCMP_Mease_sf
IPR000398 Thymidylate_synthase
IPR020940 Thymidylate_synthase_AS
PfamiView protein in Pfam
PF00303 Thymidylat_synt, 1 hit
PRINTSiPR00108 THYMDSNTHASE
SUPFAMiSSF55831 SSF55831, 1 hit
TIGRFAMsiTIGR03284 thym_sym, 1 hit
PROSITEiView protein in PROSITE
PS00091 THYMIDYLATE_SYNTHASE, 1 hit

Sequencei

Sequence statusi: Complete.

A5UDH0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQYLELCRR IVSEGEWVAN ERTGKRCLTV INADLEYDVA NNQFPLITTR
60 70 80 90 100
KSYWKAAIAE FLGYIRGYDN AADFRALGTK TWDANANENA AWLANPHRRG
110 120 130 140 150
VDDMGRVYGV QGRAWRKPNG ETIDQLRKIV NNLTKGIDDR GEILTFFNPG
160 170 180 190 200
EFDLGCLRPC MHTHTFSLVG DTLHLTSYQR SCDVPLGLNF NQIQVFTFLA
210 220 230 240 250
LMAQITGKKA GKAYHKIVNA HIYEDQLELM RDVQLKREPF PLPKLEINPD
260 270 280
IKTLEDLETW VTMDDFKVVG YQSHEPIKYP FSV
Length:283
Mass (Da):32,479
Last modified:July 10, 2007 - v1
Checksum:iC1B34C5651B8D033
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000671 Genomic DNA Translation: ABQ98821.1

Genome annotation databases

EnsemblBacteriaiABQ98821; ABQ98821; CGSHiEE_07490
KEGGihip:CGSHiEE_07490

Similar proteinsi

Entry informationi

Entry nameiTYSY_HAEIE
AccessioniPrimary (citable) accession number: A5UDH0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 10, 2007
Last modified: February 28, 2018
This is version 64 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

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