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Entry version 69 (02 Jun 2021)
Sequence version 1 (10 Jul 2007)
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Protein

Polyphosphate glucokinase

Gene

ppgK

Organism
Mycobacterium tuberculosis (strain ATCC 25177 / H37Ra)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes the phosphorylation of glucose using polyphosphate or ATP as the phosphoryl donor (PubMed:8381043, PubMed:8703950).

Polyphosphate, rather than ATP, seems to be the major phosphate donor for the enzyme in M.tuberculosis (PubMed:8703950).

GTP, UTP and CTP can replace ATP as phosphoryl donor (PubMed:8381043).

2 Publications

Miscellaneous

The poly(P)- and ATP-dependent glucokinase reactions both follow an ordered Bi-Bi mechanism, with glucose being the second substrate to bind and glucose 6-phosphate being released last. The mechanism of poly(P) utilization is not strictly processive and is most likely nonprocessive, where there is dissociation of poly(P) prior to complete utilization.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

kcat is 199 sec(-1) with polyphosphate type 35 (Sigma) as substrate at pH 7.5. kcat is 208 sec(-1) with polyphosphate type 35 (Sigma) as substrate at pH 8.6. kcat is 108 sec(-1) with ATP as substrate at pH 7.5. kcat is 116 sec(-1) with ATP as substrate at pH 8.6 (PubMed:8703950). kcat is 149 sec(-1) with poly(P)(32) as substrate at pH 8.6. kcat is 60 sec(-1) with ATP as substrate at pH 8.6. kcat is 60 sec(-1) with GTP as substrate at pH 8.6. kcat is 61 sec(-1) with dATP as substrate at pH 8.6. kcat is 37 sec(-1) with UTP as substrate at pH 8.6. kcat is 19 sec(-1) with CTP as substrate at pH 8.6 (PubMed:8381043).2 Publications
  1. KM=18.4 µM for (Phosphate)(32) (at pH 8.6)1 Publication
  2. KM=13.9 µM for polyphosphate type 35 (Sigma) (at pH 7.5)1 Publication
  3. KM=6.1 µM for polyphosphate type 35 (Sigma) (at pH 8.6)1 Publication
  4. KM=0.28 mM for D-glucose (at pH 7.5, in the polyphosphate-dependent glucokinase reaction)1 Publication
  5. KM=0.37 mM for D-glucose (at pH 8.6, in the polyphosphate-dependent glucokinase reaction)1 Publication
  6. KM=0.06 mM for D-glucose (at pH 7.5, in the ATP-dependent glucokinase reaction)1 Publication
  7. KM=0.22 mM for D-glucose (at pH 8.6, in the ATP-dependent glucokinase reaction)1 Publication
  8. KM=0.88 mM for ATP (at pH 7.5)1 Publication
  9. KM=1.4 mM for ATP (at pH 8.6)1 Publication
  10. KM=1.46 mM for ATP (at pH 8.6)1 Publication
  11. KM=0.29 mM for GTP (at pH 8.6)1 Publication
  12. KM=1.43 mM for dATP (at pH 8.6)1 Publication
  13. KM=2.19 mM for UTP (at pH 8.6)1 Publication
  14. KM=8.32 mM for CTP (at pH 8.6)1 Publication

    pH dependencei

    Optimum pH is 9.5 and 9.6-9.5 with poly(P)(32) and ATP as the phosphoryl donors, respectively.1 Publication

    Temperature dependencei

    Optimum temperature is 50 degrees Celsius for both activities.1 Publication

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi24 – 29ATPSequence analysis6

    <p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

    <p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

    Molecular functionKinase, Transferase
    LigandATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    SABIO-RK: Biochemical Reaction Kinetics Database

    More...
    SABIO-RKi
    A5U654

    <p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
    Recommended name:
    Polyphosphate glucokinase1 Publication (EC:2.7.1.632 Publications)
    Short name:
    Poly(P) glucokinase1 Publication
    Alternative name(s):
    ATP-dependent glucokinase1 Publication (EC:2.7.1.22 Publications)
    Polyphosphate--glucose phosphotransferase
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
    Name:ppgK
    Ordered Locus Names:MRA_2730
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiMycobacterium tuberculosis (strain ATCC 25177 / H37Ra)
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri419947 [NCBI]
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
    • UP000001988 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome

    <p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00003064191 – 265Polyphosphate glucokinaseAdd BLAST265

    <p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

    <p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

    Homodimer.

    1 Publication

    Protein-protein interaction databases

    STRING: functional protein association networks

    More...
    STRINGi
    419947.MRA_2730

    <p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

    3D structure databases

    SWISS-MODEL Repository - a database of annotated 3D protein structure models

    More...
    SMRi
    A5U654

    Database of comparative protein structure models

    More...
    ModBasei
    Search...

    <p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni1 – 22DisorderedSequence analysisAdd BLAST22

    Compositional bias

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'Family and Domains' section describes the position of regions of compositional bias within the protein and the particular type of amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi1 – 16Polar residuesSequence analysisAdd BLAST16

    <p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

    Belongs to the ROK (NagC/XylR) family.Curated

    Phylogenomic databases

    evolutionary genealogy of genes: Non-supervised Orthologous Groups

    More...
    eggNOGi
    COG1940, Bacteria

    The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

    More...
    HOGENOMi
    CLU_065796_0_0_11

    Identification of Orthologs from Complete Genome Data

    More...
    OMAi
    HVEMLFS

    Database of Orthologous Groups

    More...
    OrthoDBi
    1130699at2

    Family and domain databases

    Integrated resource of protein families, domains and functional sites

    More...
    InterProi
    View protein in InterPro
    IPR043129, ATPase_NBD
    IPR000600, ROK

    The PANTHER Classification System

    More...
    PANTHERi
    PTHR18964, PTHR18964, 1 hit

    Pfam protein domain database

    More...
    Pfami
    View protein in Pfam
    PF00480, ROK, 1 hit

    Superfamily database of structural and functional annotation

    More...
    SUPFAMi
    SSF53067, SSF53067, 1 hit

    <p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

    <p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

    A5U654-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MTSTGPETSE TPGATTQRHG FGIDVGGSGI KGGIVDLDTG QLIGDRIKLL
    60 70 80 90 100
    TPQPATPLAV AKTIAEVVNG FGWRGPLGVT YPGVVTHGVV RTAANVDKSW
    110 120 130 140 150
    IGTNARDTIG AELGGQQVTI LNDADAAGLA ETRYGAGKNN PGLVVLLTFG
    160 170 180 190 200
    TGIGSAVIHN GTLIPNTEFG HLEVGGKEAE ERAASSVKEK NDWTYPKWAK
    210 220 230 240 250
    QVIRVLIAIE NAIWPDLFIA GGGISRKADK WVPLLENRTP VVPAALQNTA
    260
    GIVGAAMASV ADTTH
    Length:265
    Mass (Da):27,429
    Last modified:July 10, 2007 - v1
    <p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i2F4AFAFAAC3C01E5
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti19H → G AA sequence (PubMed:8617763).Curated1
    Sequence conflicti23I → V AA sequence (PubMed:8617763).Curated1
    Sequence conflicti39T → G AA sequence (PubMed:8617763).Curated1
    Sequence conflicti41 – 42QL → EI AA sequence (PubMed:8617763).Curated2
    Sequence conflicti120I → V AA sequence (PubMed:8617763).Curated1
    Sequence conflicti132 – 133TR → EH AA sequence (PubMed:8617763).Curated2
    Sequence conflicti173 – 175EVG → QDV AA sequence (PubMed:8617763).Curated3

    Sequence databases

    Select the link destinations:

    EMBL nucleotide sequence database

    More...
    EMBLi

    GenBank nucleotide sequence database

    More...
    GenBanki

    DNA Data Bank of Japan; a nucleotide sequence database

    More...
    DDBJi
    Links Updated
    CP000611 Genomic DNA Translation: ABQ74504.1

    NCBI Reference Sequences

    More...
    RefSeqi
    WP_003911949.1, NZ_CP016972.1

    Genome annotation databases

    Ensembl bacterial and archaeal genome annotation project

    More...
    EnsemblBacteriai
    ABQ74504; ABQ74504; MRA_2730

    KEGG: Kyoto Encyclopedia of Genes and Genomes

    More...
    KEGGi
    mra:MRA_2730

    <p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

    <p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CP000611 Genomic DNA Translation: ABQ74504.1
    RefSeqiWP_003911949.1, NZ_CP016972.1

    3D structure databases

    SMRiA5U654
    ModBaseiSearch...

    Protein-protein interaction databases

    STRINGi419947.MRA_2730

    Genome annotation databases

    EnsemblBacteriaiABQ74504; ABQ74504; MRA_2730
    KEGGimra:MRA_2730

    Phylogenomic databases

    eggNOGiCOG1940, Bacteria
    HOGENOMiCLU_065796_0_0_11
    OMAiHVEMLFS
    OrthoDBi1130699at2

    Enzyme and pathway databases

    SABIO-RKiA5U654

    Family and domain databases

    InterProiView protein in InterPro
    IPR043129, ATPase_NBD
    IPR000600, ROK
    PANTHERiPTHR18964, PTHR18964, 1 hit
    PfamiView protein in Pfam
    PF00480, ROK, 1 hit
    SUPFAMiSSF53067, SSF53067, 1 hit

    MobiDB: a database of protein disorder and mobility annotations

    More...
    MobiDBi
    Search...

    <p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

    <p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiPPGK_MYCTA
    <p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: A5U654
    <p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 2, 2007
    Last sequence update: July 10, 2007
    Last modified: June 2, 2021
    This is version 69 of the entry and version 1 of the sequence. See complete history.
    <p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    <p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
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