UniProtKB - A5U3Q3 (A85B_MYCTA)
Diacylglycerol acyltransferase/mycolyltransferase Ag85B
fbpB
Functioni
The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria for fibronectin, a large adhesive glycoprotein, which facilitates the attachment of M.tuberculosis to murine alveolar macrophages (AMs). They also help to maintain the integrity of the cell wall by catalyzing the transfer of mycolic acids to cell wall arabinogalactan and through the synthesis of alpha,alpha-trehalose dimycolate (TDM, cord factor). They catalyze the transfer of a mycoloyl residue from one molecule of alpha,alpha-trehalose monomycolate (TMM) to another TMM, leading to the formation of TDM (By similarity).
By similarityCatalytic activityi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 166 | NucleophileBy similarity | 1 | |
Binding sitei | 166 | SubstrateBy similarity | 1 | |
Binding sitei | 194 | SubstrateBy similarity | 1 | |
Active sitei | 270 | By similarity | 1 | |
Binding sitei | 279 | SubstrateBy similarity | 1 | |
Active sitei | 302 | By similarity | 1 |
GO - Molecular functioni
- diacylglycerol O-acyltransferase activity Source: UniProtKB-EC
- trehalose O-mycolyltransferase activity Source: UniProtKB-EC
Keywordsi
Molecular function | Acyltransferase, Transferase |
Protein family/group databases
ESTHERi | myctu-a85b, A85-Mycolyl-transferase |
Names & Taxonomyi
Protein namesi | Recommended name: Diacylglycerol acyltransferase/mycolyltransferase Ag85B (EC:2.3.1.122, EC:2.3.1.20)Short name: DGAT Alternative name(s): 30 kDa extracellular protein Acyl-CoA:diacylglycerol acyltransferase Antigen 85 complex B Short name: 85B Short name: Ag85B Extracellular alpha-antigen Fibronectin-binding protein B Short name: Fbps B |
Gene namesi | Name:fbpB Ordered Locus Names:MRA_1897 |
Organismi | Mycobacterium tuberculosis (strain ATCC 25177 / H37Ra) |
Taxonomic identifieri | 419947 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
Subcellular locationi
Extracellular region or secreted
- Secreted By similarity
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 40 | Sequence analysisAdd BLAST | 40 | |
ChainiPRO_0000300058 | 41 – 325 | Diacylglycerol acyltransferase/mycolyltransferase Ag85BAdd BLAST | 285 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 127 ↔ 132 | By similarity |
Keywords - PTMi
Disulfide bondFamily & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 82 – 83 | Substrate bindingBy similarity | 2 | |
Regioni | 98 – 108 | Fibronectin-bindingBy similarityAdd BLAST | 11 | |
Regioni | 272 – 275 | Substrate bindingBy similarity | 4 | |
Regioni | 302 – 304 | Substrate bindingBy similarity | 3 |
Sequence similaritiesi
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | COG0627, Bacteria |
HOGENOMi | CLU_026624_3_1_11 |
OMAi | GANMPAE |
OrthoDBi | 1828423at2 |
Family and domain databases
Gene3Di | 3.40.50.1820, 1 hit |
InterProi | View protein in InterPro IPR029058, AB_hydrolase IPR000801, Esterase-like |
Pfami | View protein in Pfam PF00756, Esterase, 1 hit |
SUPFAMi | SSF53474, SSF53474, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MTDVSRKIRA WGRRLMIGTA AAVVLPGLVG LAGGAATAGA FSRPGLPVEY
60 70 80 90 100
LQVPSPSMGR DIKVQFQSGG NNSPAVYLLD GLRAQDDYNG WDINTPAFEW
110 120 130 140 150
YYQSGLSIVM PVGGQSSFYS DWYSPACGKA GCQTYKWETF LTSELPQWLS
160 170 180 190 200
ANRAVKPTGS AAIGLSMAGS SAMILAAYHP QQFIYAGSLS ALLDPSQGMG
210 220 230 240 250
PSLIGLAMGD AGGYKAADMW GPSSDPAWER NDPTQQIPKL VANNTRLWVY
260 270 280 290 300
CGNGTPNELG GANIPAEFLE NFVRSSNLKF QDAYNAAGGH NAVFNFPPNG
310 320
THSWEYWGAQ LNAMKGDLQS SLGAG
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000611 Genomic DNA Translation: ABQ73653.1 AF198032 Genomic DNA Translation: AAF13448.1 |
RefSeqi | WP_003409456.1, NZ_CP016972.1 |
Genome annotation databases
EnsemblBacteriai | ABQ73653; ABQ73653; MRA_1897 |
GeneIDi | 45425859 |
KEGGi | mra:MRA_1897 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000611 Genomic DNA Translation: ABQ73653.1 AF198032 Genomic DNA Translation: AAF13448.1 |
RefSeqi | WP_003409456.1, NZ_CP016972.1 |
3D structure databases
SMRi | A5U3Q3 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 419947.MRA_1897 |
Protein family/group databases
ESTHERi | myctu-a85b, A85-Mycolyl-transferase |
Genome annotation databases
EnsemblBacteriai | ABQ73653; ABQ73653; MRA_1897 |
GeneIDi | 45425859 |
KEGGi | mra:MRA_1897 |
Phylogenomic databases
eggNOGi | COG0627, Bacteria |
HOGENOMi | CLU_026624_3_1_11 |
OMAi | GANMPAE |
OrthoDBi | 1828423at2 |
Family and domain databases
Gene3Di | 3.40.50.1820, 1 hit |
InterProi | View protein in InterPro IPR029058, AB_hydrolase IPR000801, Esterase-like |
Pfami | View protein in Pfam PF00756, Esterase, 1 hit |
SUPFAMi | SSF53474, SSF53474, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | A85B_MYCTA | |
Accessioni | A5U3Q3Primary (citable) accession number: A5U3Q3 Secondary accession number(s): P31952, Q9RMI0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 11, 2007 |
Last sequence update: | July 10, 2007 | |
Last modified: | September 29, 2021 | |
This is version 65 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |