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Protein

S-methyl-5'-thioadenosine phosphorylase

Gene

pnp

Organism
Mycobacterium tuberculosis (strain ATCC 25177 / H37Ra)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.UniRule annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Catalytic activityi

S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate.UniRule annotation

Pathwayi: L-methionine biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (phosphorylase route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. S-methyl-5'-thioadenosine phosphorylase (pnp)
This subpathway is part of the pathway L-methionine biosynthesis via salvage pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (phosphorylase route), the pathway L-methionine biosynthesis via salvage pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei14PhosphateUniRule annotation1
Sitei162Important for substrate specificityUniRule annotation1
Binding sitei180Substrate; via amide nitrogenUniRule annotation1
Binding sitei181PhosphateUniRule annotation1
Sitei217Important for substrate specificityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferaseUniRule annotation, Transferase
Biological processPurine salvageUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00904; UER00873

Names & Taxonomyi

Protein namesi
Recommended name:
S-methyl-5'-thioadenosine phosphorylaseUniRule annotation (EC:2.4.2.28UniRule annotation)
Alternative name(s):
5'-methylthioadenosine phosphorylaseUniRule annotation
Short name:
MTA phosphorylaseUniRule annotation
Short name:
MTAPUniRule annotation
Gene namesi
Name:pnpImported
Synonyms:mtnPUniRule annotation
Ordered Locus Names:MRA_0542Imported
OrganismiMycobacterium tuberculosis (strain ATCC 25177 / H37Ra)Imported
Taxonomic identifieri419947 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001988 Componenti: Chromosome

Interactioni

Subunit structurei

Homohexamer. Dimer of a homotrimer.UniRule annotation

Protein-protein interaction databases

STRINGi419947.MRA_0542

Structurei

3D structure databases

ProteinModelPortaliA5TZR5
SMRiA5TZR5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini9 – 239PNP_UDP_1InterPro annotationAdd BLAST231

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni55 – 56Phosphate bindingUniRule annotation2
Regioni204 – 206Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107USC Bacteria
COG0005 LUCA
HOGENOMiHOG000228987
KOiK00772
OMAiNLYRSWG

Family and domain databases

HAMAPiMF_01963 MTAP, 1 hit
InterProiView protein in InterPro
IPR010044 MTAP
IPR000845 Nucleoside_phosphorylase_d
IPR035994 Nucleoside_phosphorylase_sf
PANTHERiPTHR42679 PTHR42679, 1 hit
PfamiView protein in Pfam
PF01048 PNP_UDP_1, 1 hit
SUPFAMiSSF53167 SSF53167, 1 hit
TIGRFAMsiTIGR01694 MTAP, 1 hit

Sequencei

Sequence statusi: Complete.

A5TZR5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHNNGRMLGV IGGSGFYTFF GSDTRTVNSD TPYGQPSAPI TIGTIGVHDV
60 70 80 90 100
AFLPRHGAHH QYSAHAVPYR ANMWALRALG VRRVFGPCAV GSLDPELEPG
110 120 130 140 150
AVVVPDQLVD RTSGRADTYF DFGGVHAAFA DPYCPTLRAA VTGLPGVVDG
160 170 180 190 200
GTMVVIQGPR FSTRAESQWF AAAGCNLVNM TGYPEAVLAR ELELCYAAIA
210 220 230 240 250
LVTDVDAGVA AGDGVKAADV FAAFGENIEL LKRLVRAAID RVADERTCTH
260
CQHHAGVPLP FELP
Length:264
Mass (Da):27,964
Last modified:July 10, 2007 - v1
Checksum:i3A850EA1B1873662
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000611 Genomic DNA Translation: ABQ72265.1
RefSeqiWP_003402867.1, NZ_CP016972.1

Genome annotation databases

EnsemblBacteriaiABQ72265; ABQ72265; MRA_0542
KEGGimra:MRA_0542

Similar proteinsi

Entry informationi

Entry nameiA5TZR5_MYCTA
AccessioniPrimary (citable) accession number: A5TZR5
Entry historyiIntegrated into UniProtKB/TrEMBL: July 10, 2007
Last sequence update: July 10, 2007
Last modified: October 25, 2017
This is version 68 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

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