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UniProtKB - A5TY85 (PKNA_MYCTA)
Protein
Serine/threonine-protein kinase PknA
Gene
pknA
Organism
Mycobacterium tuberculosis (strain ATCC 25177 / H37Ra)
Status
Functioni
Protein kinase that regulates many aspects of mycobacterial physiology. Is a key component of a signal transduction pathway that regulates cell growth, cell shape and cell division via phosphorylation of target proteins such as FtsZ and MurD. Shows a strong preference for Thr versus Ser as the phosphoacceptor.
3 PublicationsCatalytic activityi
Activity regulationi
Activated by magnesium or manganese. Inhibited by vanadate.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 42 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 141 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 19 – 27 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- protein serine/threonine/tyrosine kinase activity Source: RHEA
- protein serine/threonine kinase activity Source: UniProtKB-KW
- protein serine kinase activity Source: RHEA
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:pknA Ordered Locus Names:MRA_0017 |
Organismi | Mycobacterium tuberculosis (strain ATCC 25177 / H37Ra) |
Taxonomic identifieri | 419947 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Single-pass membrane protein By similarity
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 339 | CytoplasmicSequence analysisAdd BLAST | 339 | |
Transmembranei | 340 – 360 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 361 – 431 | ExtracellularSequence analysisAdd BLAST | 71 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 42 | K → M: Lack of autophosphorylation. 2 Publications | 1 | |
Mutagenesisi | 172 | T → A: Strong decrease in autophosphorylation. 1 Publication | 1 | |
Mutagenesisi | 174 | T → A: Decrease in autophosphorylation. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000419742 | 1 – 431 | Serine/threonine-protein kinase PknAAdd BLAST | 431 |
Post-translational modificationi
Autophosphorylated.
Keywords - PTMi
PhosphoproteinInteractioni
Subunit structurei
Interacts with FtsZ and MurD.
2 PublicationsProtein-protein interaction databases
STRINGi | 419947.MRA_0017 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 13 – 272 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 260 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 276 – 333 | DisorderedSequence analysisAdd BLAST | 58 | |
Regioni | 366 – 418 | DisorderedSequence analysisAdd BLAST | 53 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 302 – 316 | Polar residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 366 – 394 | Polar residuesSequence analysisAdd BLAST | 29 | |
Compositional biasi | 403 – 417 | Polar residuesSequence analysisAdd BLAST | 15 |
Domaini
The kinase domain and the juxtamembrane region constitute the catalytic core of PknA. Interaction between core and C-terminal domains is crucial for activity.1 Publication
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG0515, Bacteria |
HOGENOMi | CLU_000288_63_44_11 |
OMAi | CPGAPTT |
OrthoDBi | 1377603at2 |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
i Sequence
Sequence statusi: Complete.
A5TY85-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSPRVGVTLS GRYRLQRLIA TGGMGQVWEA VDNRLGRRVA VKVLKSEFSS
60 70 80 90 100
DPEFIERFRA EARTTAMLNH PGIASVHDYG ESQMNGEGRT AYLVMELVNG
110 120 130 140 150
EPLNSVLKRT GRLSLRHALD MLEQTGRALQ IAHAAGLVHR DVKPGNILIT
160 170 180 190 200
PTGQVKITDF GIAKAVDAAP VTQTGMVMGT AQYIAPEQAL GHDASPASDV
210 220 230 240 250
YSLGVVGYEA VSGKRPFAGD GALTVAMKHI KEPPPPLPPD LPPNVRELIE
260 270 280 290 300
ITLVKNPAMR YRSGGPFADA VAAVRAGRRP PRPSQTPPPG RAAPAAIPSG
310 320 330 340 350
TTARVAANSA GRTAASRRSR PATGGHRPPR RTFSSGQRAL LWAAGVLGAL
360 370 380 390 400
AIIIAVLLVI KAPGDNSPQQ APTPTVTTTG NPPASNTGGT DASPRLNWTE
410 420 430
RGETRHSGLQ SWVVPPTPHS RASLARYEIA Q
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000611 Genomic DNA Translation: ABQ71735.1 |
RefSeqi | WP_003400358.1, NZ_CP016972.1 |
Genome annotation databases
EnsemblBacteriai | ABQ71735; ABQ71735; MRA_0017 |
GeneIDi | 45423974 |
KEGGi | mra:MRA_0017 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000611 Genomic DNA Translation: ABQ71735.1 |
RefSeqi | WP_003400358.1, NZ_CP016972.1 |
3D structure databases
SMRi | A5TY85 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 419947.MRA_0017 |
Genome annotation databases
EnsemblBacteriai | ABQ71735; ABQ71735; MRA_0017 |
GeneIDi | 45423974 |
KEGGi | mra:MRA_0017 |
Phylogenomic databases
eggNOGi | COG0515, Bacteria |
HOGENOMi | CLU_000288_63_44_11 |
OMAi | CPGAPTT |
OrthoDBi | 1377603at2 |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PKNA_MYCTA | |
Accessioni | A5TY85Primary (citable) accession number: A5TY85 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 31, 2012 |
Last sequence update: | July 10, 2007 | |
Last modified: | February 23, 2022 | |
This is version 83 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |