UniProtKB - A5PN09 (UBP20_DANRE)
Ubiquitin carboxyl-terminal hydrolase 20
usp20
Functioni
Deubiquitinating enzyme involved in beta-2 adrenergic receptor (adrb2) recycling. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination beta-2 adrenergic receptor (adrb2). Plays a central role in adrb2 recycling and resensitization after prolonged agonist stimulation by constitutively binding adrb2, mediating deubiquitination of adrb2 and inhibiting lysosomal trafficking of adrb2. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (By similarity).
By similarityCatalytic activityi
- Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal). EC:3.4.19.12
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 8 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 10 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 30 | Zinc 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 33 | Zinc 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 43 | Zinc 3PROSITE-ProRule annotation | 1 | |
Metal bindingi | 48 | Zinc 3PROSITE-ProRule annotation | 1 | |
Metal bindingi | 53 | Zinc 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 60 | Zinc 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 64 | Zinc 3PROSITE-ProRule annotation | 1 | |
Metal bindingi | 70 | Zinc 3PROSITE-ProRule annotation | 1 | |
Metal bindingi | 83 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 86 | Zinc 1PROSITE-ProRule annotation | 1 | |
Active sitei | 157 | NucleophilePROSITE-ProRule annotation | 1 | |
Active sitei | 643 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 6 – 108 | UBP-typePROSITE-ProRule annotationAdd BLAST | 103 |
GO - Molecular functioni
- cysteine-type endopeptidase activity Source: UniProtKB
- thiol-dependent deubiquitinase Source: UniProtKB
- zinc ion binding Source: InterPro
GO - Biological processi
- central nervous system morphogenesis Source: ZFIN
- cranial skeletal system development Source: ZFIN
- endocytosis Source: UniProtKB-KW
- protein deubiquitination Source: UniProtKB
- protein K48-linked deubiquitination Source: UniProtKB
- protein K63-linked deubiquitination Source: UniProtKB
- regulation of G protein-coupled receptor signaling pathway Source: UniProtKB
- ubiquitin-dependent protein catabolic process Source: InterPro
Keywordsi
Molecular function | Hydrolase, Protease, Thiol protease |
Biological process | Endocytosis, Ubl conjugation pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-DRE-5689880, Ub-specific processing proteases |
Protein family/group databases
MEROPSi | C19.025 |
Names & Taxonomyi
Protein namesi | Recommended name: Ubiquitin carboxyl-terminal hydrolase 20 (EC:3.4.19.12)Alternative name(s): Deubiquitinating enzyme 20 Ubiquitin thioesterase 20 Ubiquitin-specific-processing protease 20 |
Gene namesi | Name:usp20 ORF Names:dkey-125i20.3 |
Organismi | Danio rerio (Zebrafish) (Brachydanio rerio) |
Taxonomic identifieri | 7955 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Danionidae › Danioninae › Danio |
Proteomesi |
|
Organism-specific databases
ZFINi | ZDB-GENE-040426-1219, usp20 |
Subcellular locationi
Cytoskeleton
- centrosome By similarity
Other locations
- perinuclear region By similarity
Cytoskeleton
- centrosome Source: UniProtKB
Other locations
- cytoplasm Source: GO_Central
- perinuclear region of cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cytoplasm, CytoskeletonPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000390420 | 1 – 911 | Ubiquitin carboxyl-terminal hydrolase 20Add BLAST | 911 |
Proteomic databases
PaxDbi | A5PN09 |
Structurei
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 148 – 685 | USPAdd BLAST | 538 | |
Domaini | 687 – 780 | DUSP 1PROSITE-ProRule annotationAdd BLAST | 94 | |
Domaini | 789 – 891 | DUSP 2PROSITE-ProRule annotationAdd BLAST | 103 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 279 – 309 | DisorderedSequence analysisAdd BLAST | 31 | |
Regioni | 327 – 420 | DisorderedSequence analysisAdd BLAST | 94 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 327 – 357 | Basic and acidic residuesSequence analysisAdd BLAST | 31 | |
Compositional biasi | 382 – 420 | Polar residuesSequence analysisAdd BLAST | 39 |
Domaini
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 6 – 108 | UBP-typePROSITE-ProRule annotationAdd BLAST | 103 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG1870, Eukaryota |
GeneTreei | ENSGT00940000158829 |
InParanoidi | A5PN09 |
OrthoDBi | 147564at2759 |
PhylomeDBi | A5PN09 |
Family and domain databases
Gene3Di | 3.30.2230.10, 2 hits 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR035927, DUSP-like_sf IPR038765, Papain-like_cys_pep_sf IPR006615, Pept_C19_DUSP IPR001394, Peptidase_C19_UCH IPR018200, USP_CS IPR028889, USP_dom IPR013083, Znf_RING/FYVE/PHD IPR001607, Znf_UBP |
Pfami | View protein in Pfam PF06337, DUSP, 2 hits PF00443, UCH, 2 hits PF02148, zf-UBP, 1 hit |
SMARTi | View protein in SMART SM00695, DUSP, 2 hits SM00290, ZnF_UBP, 1 hit |
SUPFAMi | SSF143791, SSF143791, 2 hits SSF54001, SSF54001, 1 hit |
PROSITEi | View protein in PROSITE PS51283, DUSP, 2 hits PS00972, USP_1, 1 hit PS00973, USP_2, 1 hit PS50235, USP_3, 1 hit PS50271, ZF_UBP, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MTDSGDLCPH LDSIGEVTKE ELIQKSKGTC QSCGVGGPNL WACLQCDCPY
60 70 80 90 100
VGCGESYSDH STIHAQAKKH NLTVNLTTFR VWCYVCEREV FLEPKPVTPV
110 120 130 140 150
SSAHRCKPHD QDPVSQTTCY PLKAVPIAVA DEEGSESEED ELKPRGLTGM
160 170 180 190 200
KNIGNSCYMN AALQALSNCP PLTQFFQDCS GLVRTDKKPA LCKSYQKLIS
210 220 230 240 250
ELWHKKRPSY VVPTTLFHGI KLVNPMFRGY AQQDTQEFLR CLMDQLHEEL
260 270 280 290 300
KEPLFDCSGG ISEVEPDLSL DSCNLVDGDR SPSEDEFLSC DSGSGSERGD
310 320 330 340 350
GERAGGEAEL LIQDECVAVR GTGGISEKER LKERRGEERT REMDEDADVD
360 370 380 390 400
TAAQDGQAER ETETATPATA VPAPGNTEPD NEASMHCPSS RPCSPAHSVQ
410 420 430 440 450
ELHSRLSSNP PRSSPLRTGP TYTFKKAQML LSTKKKKQSR FRSVISDIFD
460 470 480 490 500
GSILSLVQCL TCDRVSTTVE TFQDLSLPIP GKEDLAKLHS SIHQSAPVKA
510 520 530 540 550
GVCTDGYAAQ GWISYIMDSI RRFVVSCIPS WFWGPMVTLE DCLAAFFAAD
560 570 580 590 600
ELKGDNMYSC ERCKKLRNGV KYCKVLRLPE ILCIHLKRFR HEVMYSFKIN
610 620 630 640 650
SHVSFPLEGL DLKPFLAKES PSQITTYDLL SVICHHGTAG SGHYIAYCQN
660 670 680 690 700
VINGQWYEFD DQYVTEVHET VVQNAEAYVL FYRKSSEESV RERQRVVALA
710 720 730 740 750
NLKEPSLLQF YISREWLNKF NTFTEPGPIT NHTFLCQHGG IPPTKYHYVD
760 770 780 790 800
DLVVILPQNV WEYLYNRFGG GPAVNHLYVC AICQVEIETL AKRRKLEIDT
810 820 830 840 850
FIKLNKEFQA EEAPTVILCI SMQWFREWEN FVKGKDNEPP GPIDNSKIAV
860 870 880 890 900
MKGGHIQLKQ GADYGQISEE TWQYLLSIYG GGPEIAVRQT ISPPDTDTHG
910
ERKIEAETRA L
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE7F0E7 | E7F0E7_DANRE | Ubiquitinyl hydrolase 1 | usp20 | 373 | Annotation score: | ||
A0A0R4IBG7 | A0A0R4IBG7_DANRE | Ubiquitin carboxyl-terminal hydrola... | usp20 | 85 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 115 | S → L in CAN88765 (PubMed:23594743).Curated | 1 | |
Sequence conflicti | 115 | S → L in CAN88088 (PubMed:23594743).Curated | 1 | |
Sequence conflicti | 429 | M → I in CAN88765 (PubMed:23594743).Curated | 1 | |
Sequence conflicti | 429 | M → I in CAN88088 (PubMed:23594743).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BX927184 Genomic DNA Translation: CAN88489.1 BX005029, CR382323 Genomic DNA Translation: CAN88088.1 CR382323, BX005029 Genomic DNA Translation: CAN88765.1 Sequence problems. |
RefSeqi | NP_957281.2, NM_200987.2 XP_009299733.1, XM_009301458.2 |
Genome annotation databases
Ensembli | ENSDART00000006021; ENSDARP00000014964; ENSDARG00000027501 |
GeneIDi | 393962 |
KEGGi | dre:393962 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BX927184 Genomic DNA Translation: CAN88489.1 BX005029, CR382323 Genomic DNA Translation: CAN88088.1 CR382323, BX005029 Genomic DNA Translation: CAN88765.1 Sequence problems. |
RefSeqi | NP_957281.2, NM_200987.2 XP_009299733.1, XM_009301458.2 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 7955.ENSDARP00000014964 |
Protein family/group databases
MEROPSi | C19.025 |
Proteomic databases
PaxDbi | A5PN09 |
Genome annotation databases
Ensembli | ENSDART00000006021; ENSDARP00000014964; ENSDARG00000027501 |
GeneIDi | 393962 |
KEGGi | dre:393962 |
Organism-specific databases
CTDi | 10868 |
ZFINi | ZDB-GENE-040426-1219, usp20 |
Phylogenomic databases
eggNOGi | KOG1870, Eukaryota |
GeneTreei | ENSGT00940000158829 |
InParanoidi | A5PN09 |
OrthoDBi | 147564at2759 |
PhylomeDBi | A5PN09 |
Enzyme and pathway databases
Reactomei | R-DRE-5689880, Ub-specific processing proteases |
Miscellaneous databases
PROi | PR:A5PN09 |
Gene expression databases
ExpressionAtlasi | A5PN09, baseline |
Family and domain databases
Gene3Di | 3.30.2230.10, 2 hits 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR035927, DUSP-like_sf IPR038765, Papain-like_cys_pep_sf IPR006615, Pept_C19_DUSP IPR001394, Peptidase_C19_UCH IPR018200, USP_CS IPR028889, USP_dom IPR013083, Znf_RING/FYVE/PHD IPR001607, Znf_UBP |
Pfami | View protein in Pfam PF06337, DUSP, 2 hits PF00443, UCH, 2 hits PF02148, zf-UBP, 1 hit |
SMARTi | View protein in SMART SM00695, DUSP, 2 hits SM00290, ZnF_UBP, 1 hit |
SUPFAMi | SSF143791, SSF143791, 2 hits SSF54001, SSF54001, 1 hit |
PROSITEi | View protein in PROSITE PS51283, DUSP, 2 hits PS00972, USP_1, 1 hit PS00973, USP_2, 1 hit PS50235, USP_3, 1 hit PS50271, ZF_UBP, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | UBP20_DANRE | |
Accessioni | A5PN09Primary (citable) accession number: A5PN09 Secondary accession number(s): A5PM59 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 15, 2009 |
Last sequence update: | July 10, 2007 | |
Last modified: | February 23, 2022 | |
This is version 89 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - SIMILARITY comments
Index of protein domains and families