UniProtKB - A5PK26 (ACAP1_BOVIN)
Protein
Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1
Gene
ACAP1
Organism
Bos taurus (Bovine)
Status
Functioni
GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface. Required for regulated export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration (By similarity).By similarity
Activity regulationi
GAP activity stimulated by phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphatidic acid.By similarity
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 420 – 443 | C4-typePROSITE-ProRule annotationAdd BLAST | 24 |
GO - Molecular functioni
- GTPase activator activity Source: InterPro
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- signal transduction Source: UniProtKB-KW
Keywordsi
Molecular function | Transducer |
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1Alternative name(s): Centaurin-beta-1 Short name: Cnt-b1 |
Gene namesi | Name:ACAP1 Synonyms:CENTB1 |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Subcellular locationi
Endosome
- Recycling endosome membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
Endosome
- recycling endosome membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Endosome, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000306383 | 1 – 745 | Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1Add BLAST | 745 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 485 | 3'-nitrotyrosineBy similarity | 1 | |
Modified residuei | 555 | Phosphoserine; by PKBBy similarity | 1 |
Post-translational modificationi
Phosphorylation at Ser-555 by PKB is required for interaction with ITGB1, export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.By similarity
Keywords - PTMi
Nitration, PhosphoproteinProteomic databases
PaxDbi | A5PK26 |
PRIDEi | A5PK26 |
Interactioni
Subunit structurei
Banana-shaped homodimer laterally assembling into tetramers, the tetramers further pack helically onto the membrane.
Interacts with GTP-bound ARF6.
Interacts with third cytoplasmic loop of SLC2A4/GLUT4.
Interacts with CLTC.
Interacts with GULP1.
Forms a complex with GDP-bound ARF6 and GULP1.
Interacts with ITGB1; required for ITGB1 recycling (By similarity).
By similarityProtein-protein interaction databases
STRINGi | 9913.ENSBTAP00000034967 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 226 | BARSequence analysisAdd BLAST | 226 | |
Domaini | 265 – 360 | PHPROSITE-ProRule annotationAdd BLAST | 96 | |
Domaini | 405 – 527 | Arf-GAPPROSITE-ProRule annotationAdd BLAST | 123 | |
Repeati | 607 – 640 | ANK 1Sequence analysisAdd BLAST | 34 | |
Repeati | 644 – 673 | ANK 2Sequence analysisAdd BLAST | 30 | |
Repeati | 677 – 707 | ANK 3Sequence analysisAdd BLAST | 31 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 382 | Required for formation of endosomal tubules when overexpressed with PIP5K1CBy similarityAdd BLAST | 382 | |
Regioni | 405 – 745 | Required for interaction with GULP1By similarityAdd BLAST | 341 | |
Regioni | 525 – 567 | Prevents interaction with ITGB1 when S-554 is not phosphorylatedBy similarityAdd BLAST | 43 |
Domaini
PH domain binds phospholipids including phosphatidic acid, phosphatidylinositol 3-phosphate, phosphatidylinositol 3,5-bisphosphate (PIP2) and phosphatidylinositol 3,4,5-trisphosphate (PIP3). May mediate ACAP1-binding to PIP2 or PIP3 containing membranes. Only one PH domain of one ACAP1 dimer inserts into the membrane, while the other PH domain acts primaryly to interact with adjacent ACAP1 dimers (By similarity).By similarity
The BAR domain mediates homodimerization, it can neither bind membrane nor impart curvature, but instead requires the neighboring PH domain to achieve these functions (By similarity).By similarity
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 420 – 443 | C4-typePROSITE-ProRule annotationAdd BLAST | 24 |
Keywords - Domaini
ANK repeat, Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG0521, Eukaryota |
InParanoidi | A5PK26 |
OrthoDBi | 751525at2759 |
Family and domain databases
Gene3Di | 1.20.1270.60, 1 hit 1.25.40.20, 1 hit 2.30.29.30, 1 hit 3.30.40.160, 1 hit |
InterProi | View protein in InterPro IPR027267, AH/BAR_dom_sf IPR002110, Ankyrin_rpt IPR020683, Ankyrin_rpt-contain_dom IPR036770, Ankyrin_rpt-contain_sf IPR037278, ARFGAP/RecO IPR001164, ArfGAP_dom IPR038508, ArfGAP_dom_sf IPR011993, PH-like_dom_sf IPR001849, PH_domain |
Pfami | View protein in Pfam PF12796, Ank_2, 1 hit PF01412, ArfGap, 1 hit PF00169, PH, 1 hit |
PRINTSi | PR00405, REVINTRACTNG |
SMARTi | View protein in SMART SM00248, ANK, 3 hits SM00105, ArfGap, 1 hit SM00233, PH, 1 hit |
SUPFAMi | SSF103657, SSF103657, 1 hit SSF48403, SSF48403, 1 hit SSF57863, SSF57863, 1 hit |
PROSITEi | View protein in PROSITE PS50297, ANK_REP_REGION, 1 hit PS50088, ANK_REPEAT, 1 hit PS50115, ARFGAP, 1 hit PS50003, PH_DOMAIN, 1 hit |
i Sequence
Sequence statusi: Complete.
A5PK26-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTVKLDFEEC LKDSPRFRAS VELVEAEVSE LETRLEKLLK LGNGLLESGR
60 70 80 90 100
HYLAASRAFI VGICDLAHLG PPEPMMAECL DKFTQSLSHK LDSHAELLDA
110 120 130 140 150
TQHTLQRQIQ TLVKEGLRSF REAGRDFWRG AESLEAALTH NAEVPRRRAQ
160 170 180 190 200
EAEEAGAALK VARAGYRGRA LDYALQINVI EDKRKFDIME FVLRLVEAQA
210 220 230 240 250
THFQQGHEEL SQLAQYRKEL GGQLHQLVLN SAREKRDMEQ RHVLLKQKEL
260 270 280 290 300
GGEEPEPSLK EGPGGLVMEG HLFKRASNAF KTWSRRWFTI QSNQLVYQKR
310 320 330 340 350
YKDPVTVVVD DLRLCTVKLC PDSERRFCFE VVSPSKSCLL QSDSERLMQL
360 370 380 390 400
WVSAVQSSIA TAFSQARLDD SPRGLGQGSG HLAISSAATL GPGGLTRGRE
410 420 430 440 450
PGGVGHVAAQ VQSVDGNAQC CDCREPAPEW ASINLGVTLC IQCSGIHRSL
460 470 480 490 500
GVHFSKVRSL TLDSWEPELV KLMCELGNVV INQIYEARVE AMAVKKPGPS
510 520 530 540 550
CSRQEKEAWI HAKYVEKKFL TKLPEIRGRR GGRGPPRGHP PVPPKPGLIR
560 570 580 590 600
PKPGSFRSKP EPPSEDLQSL HPGALLFRAA GHPPSLPTMA DALAHGADVN
610 620 630 640 650
WVNGGQENAT PLIQATAAVR VLNSLLACEF LLQNGANVNQ VDNQGRGPLH
660 670 680 690 700
HATILGHTGL ACLFLKRGAD LGVRDSEGRD PLTIAVETAN ADIVTLLRLA
710 720 730 740
KMREADAAQG QAGDETYLDI FRDFSLMASD DPEKLSRRSH DLHTL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC142328 mRNA Translation: AAI42329.1 |
RefSeqi | NP_001096704.1, NM_001103234.1 |
Genome annotation databases
GeneIDi | 508582 |
KEGGi | bta:508582 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC142328 mRNA Translation: AAI42329.1 |
RefSeqi | NP_001096704.1, NM_001103234.1 |
3D structure databases
SMRi | A5PK26 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9913.ENSBTAP00000034967 |
Proteomic databases
PaxDbi | A5PK26 |
PRIDEi | A5PK26 |
Genome annotation databases
GeneIDi | 508582 |
KEGGi | bta:508582 |
Organism-specific databases
CTDi | 9744 |
Phylogenomic databases
eggNOGi | KOG0521, Eukaryota |
InParanoidi | A5PK26 |
OrthoDBi | 751525at2759 |
Family and domain databases
Gene3Di | 1.20.1270.60, 1 hit 1.25.40.20, 1 hit 2.30.29.30, 1 hit 3.30.40.160, 1 hit |
InterProi | View protein in InterPro IPR027267, AH/BAR_dom_sf IPR002110, Ankyrin_rpt IPR020683, Ankyrin_rpt-contain_dom IPR036770, Ankyrin_rpt-contain_sf IPR037278, ARFGAP/RecO IPR001164, ArfGAP_dom IPR038508, ArfGAP_dom_sf IPR011993, PH-like_dom_sf IPR001849, PH_domain |
Pfami | View protein in Pfam PF12796, Ank_2, 1 hit PF01412, ArfGap, 1 hit PF00169, PH, 1 hit |
PRINTSi | PR00405, REVINTRACTNG |
SMARTi | View protein in SMART SM00248, ANK, 3 hits SM00105, ArfGap, 1 hit SM00233, PH, 1 hit |
SUPFAMi | SSF103657, SSF103657, 1 hit SSF48403, SSF48403, 1 hit SSF57863, SSF57863, 1 hit |
PROSITEi | View protein in PROSITE PS50297, ANK_REP_REGION, 1 hit PS50088, ANK_REPEAT, 1 hit PS50115, ARFGAP, 1 hit PS50003, PH_DOMAIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ACAP1_BOVIN | |
Accessioni | A5PK26Primary (citable) accession number: A5PK26 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 2, 2007 |
Last sequence update: | July 10, 2007 | |
Last modified: | December 2, 2020 | |
This is version 87 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |