UniProtKB - A5K0N4 (KGP_PLAVS)
cGMP-dependent protein kinase
PKG
Functioni
Serine/threonine protein kinase which acts as a downstream effector of the second messenger cGMP. Controls the release of Ca2+ from intracellular stores by regulating phosphoinositide biosynthesis. Ca2+ signals are essential for merozoite and sporozoite invasion and egress from host hepatocytes and erythrocytes, and, in the mosquito vector, for gametocyte activation, and ookinete and sporozoite motility (By similarity).
During the host liver stage, regulates the initial invasion of host hepatocytes by sporozoites by regulating sporozoite motility and microneme exocytosis. Following parasite development in the hepatocytes, required for the release of merosomes, a vesicle containing the mature merozoites (By similarity).
During the asexual blood stage, required for the progression from schizont to the ring stage following merozoite invasion of host erythrocytes and for merozoite egress. Regulates merozoite egress by promoting the release of exonemes and micronemes which contain proteins essential for egress. Phosphorylates CDPK1 predominantly at the late schizont stage; phosphorylation at 'Ser-64' regulates CDPK1 protein-protein interaction and phosphorylation at 'Thr-231' may regulate CDPK1 kinase activity. In the mosquito vector, required for the initiation of gametogenesis induced by xanthurenic acid, specifically the gametocyte differentiation from the crescent-shaped form to the spherical form (By similarity).
Required for the gliding motility of ookinetes to reach and penetrate the midgut epithelium by promoting Ca2+-mediated activation of CDPK1 and CDPK4. Also required for microneme secretion in ookinete by promoting Ca2+-mediated activation of CDPK3 (By similarity).
By similarityCatalytic activityi
Cofactori
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 106 | 3',5'-cGMP 1; allosteric activatorBy similarity | 1 | |
Binding sitei | 115 | 3',5'-cGMP 1; via amide nitrogen; allosteric activatorBy similarity | 1 | |
Binding sitei | 116 | 3',5'-cGMP 1; allosteric activatorBy similarity | 1 | |
Binding sitei | 118 | 3',5'-cGMP 1; via amide nitrogen; allosteric activatorBy similarity | 1 | |
Binding sitei | 125 | 3',5'-cGMP 1; allosteric activatorBy similarity | 1 | |
Binding sitei | 126 | 3',5'-cGMP 1; allosteric activatorBy similarity | 1 | |
Binding sitei | 466 | 3',5'-cGMP 2; allosteric activatorBy similarity | 1 | |
Binding sitei | 475 | 3',5'-cGMP 2; via amide nitrogen; allosteric activatorBy similarity | 1 | |
Binding sitei | 476 | 3',5'-cGMP 2; allosteric activatorBy similarity | 1 | |
Sitei | 477 | Part of a catalytic triad required for cGMP binding and cGMP-dependent kinase activityBy similarity | 1 | |
Binding sitei | 478 | 3',5'-cGMP 2; via amide nitrogen; allosteric activatorBy similarity | 1 | |
Binding sitei | 485 | 3',5'-cGMP 2; allosteric activatorBy similarity | 1 | |
Binding sitei | 486 | 3',5'-cGMP 2; allosteric activatorBy similarity | 1 | |
Sitei | 525 | Part of a catalytic triad required for cGMP binding and cGMP-dependent kinase activityBy similarity | 1 | |
Sitei | 526 | Part of a catalytic triad required for cGMP binding and cGMP-dependent kinase activityBy similarity | 1 | |
Binding sitei | 563 | ATPPROSITE-ProRule annotationCombined sources1 Publication | 1 | |
Active sitei | 657 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 540 – 548 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- cGMP binding Source: UniProtKB-KW
- cGMP-dependent protein kinase activity Source: UniProtKB-EC
- metal ion binding Source: UniProtKB-KW
- protein serine/threonine/tyrosine kinase activity Source: RHEA
- protein serine kinase activity Source: RHEA
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Ligand | ATP-binding, cGMP, cGMP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:PKGCurated ORF Names:PVX_084705Imported |
Organismi | Plasmodium vivax (strain Salvador I)Imported |
Taxonomic identifieri | 126793 [NCBI] |
Taxonomic lineagei | Eukaryota › Sar › Alveolata › Apicomplexa › Aconoidasida › Haemosporida › Plasmodiidae › Plasmodium › Plasmodium (Plasmodium) › |
Proteomesi |
|
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
Cytoplasm and Cytosol
- Cytoplasm By similarity
Note: Predominantly localizes to the cytoplasm during schizogony.By similarity
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cytoplasm, Endoplasmic reticulum, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000451909 | 1 – 846 | cGMP-dependent protein kinaseAdd BLAST | 846 |
Post-translational modificationi
Interactioni
Subunit structurei
Monomer.
By similarityProtein-protein interaction databases
STRINGi | 126793.A5K0N4 |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | A5K0N4 |
SMRi | A5K0N4 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 534 – 791 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 258 | |
Domaini | 792 – 846 | AGC-kinase C-terminalPROSITE-ProRule annotationAdd BLAST | 55 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 22 | Autoinhibitory segmentBy similarityAdd BLAST | 22 | |
Regioni | 51 – 166 | cNMP-binding domain 1PROSITE-ProRule annotationAdd BLAST | 116 | |
Regioni | 169 – 268 | cNMP-binding domain 2PROSITE-ProRule annotationAdd BLAST | 100 | |
Regioni | 288 – 391 | cNMP-binding domain 3PROSITE-ProRule annotationAdd BLAST | 104 | |
Regioni | 411 – 510 | cNMP-binding domain 4PROSITE-ProRule annotationAdd BLAST | 100 | |
Regioni | 824 – 846 | DisorderedSequence analysisAdd BLAST | 23 |
Domaini
Sequence similaritiesi
Phylogenomic databases
InParanoidi | A5K0N4 |
OMAi | SKNPDGH |
Family and domain databases
CDDi | cd00038, CAP_ED, 3 hits cd05572, STKc_cGK, 1 hit |
Gene3Di | 2.60.120.10, 4 hits |
InterProi | View protein in InterPro IPR000961, AGC-kinase_C IPR002374, cGMP_dep_kinase IPR018490, cNMP-bd-like IPR018488, cNMP-bd_CS IPR000595, cNMP-bd_dom IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR014710, RmlC-like_jellyroll IPR008271, Ser/Thr_kinase_AS IPR035014, STKc_cGK |
Pfami | View protein in Pfam PF00027, cNMP_binding, 3 hits PF00069, Pkinase, 1 hit |
PIRSFi | PIRSF000559, cGMP-dep_kinase, 1 hit |
SMARTi | View protein in SMART SM00100, cNMP, 4 hits SM00220, S_TKc, 1 hit |
SUPFAMi | SSF51206, SSF51206, 4 hits SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS51285, AGC_KINASE_CTER, 1 hit PS00888, CNMP_BINDING_1, 3 hits PS00889, CNMP_BINDING_2, 3 hits PS50042, CNMP_BINDING_3, 4 hits PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MRCNERNKKK AIFSNDDFSG EDTLMEDHLQ LREKLSEDIE MIKASLKNNL
60 70 80 90 100
VCSTLNDNEI LTLSNYMQFF VFKGGDLVIK QGEKGSYFFI INSGKFDVYV
110 120 130 140 150
NDKKVKSMGK GSSFGEAALI HNTQRSATIM AETDGTLWGV QRSTFRATLK
160 170 180 190 200
QLSNRNFNEN RSFIDSVSVF DMLTEAQKNM ITNACVIQMF KPGETIVKQG
210 220 230 240 250
DYGDVLFILK EGKATVFIND KEIRVLNKGS YFGERALLYD EPRSATIIAK
260 270 280 290 300
EPTACASICR KLLNIVLGNL QVVLFRNIMT EALQQSEIFR QFSAEQLNDL
310 320 330 340 350
ADTAIVRDYP ANYHILHKDK VKSVKYLIVL EGKVELFLDD ESIGILTRGK
360 370 380 390 400
SFGDQYVLNQ KQKFRHTVKS LDVCKIALIT ESCLADCLGD NNIDASIDHN
410 420 430 440 450
NKKSIIKKMY IFRYLSEQQC NLLIEAFRTT RYEEGDYIIQ EGEVGSRFYI
460 470 480 490 500
IKNGEVEVTK NGKRLRTLGK NDYFGERALL YDEPRTASII SKATSVECWF
510 520 530 540 550
VDKSVFLQII QGPMLTHLEE RIKMQDTKVE MHELETERII GRGTFGTVKL
560 570 580 590 600
VHHKPTQIRY ALKCVSKRSI ISLNQQNNIK LEREITAEND HPFIIRLVRT
610 620 630 640 650
FKDSNCFYFL TELVTGGELY DAIRKLGLLS KPQAQFYLGS IILAIEYLHE
660 670 680 690 700
RNIVYRDLKP ENILLDKQGY VKLIDFGCAK KIQGRAYTLV GTPHYMAPEV
710 720 730 740 750
ILGKGYGCTV DIWALGVCLY EFICGPLPFG NDQEDQLEIF RDILTGQLTF
760 770 780 790 800
PDYVSDQDSI NLMKRLLCRL PQGRIGCSIN GFKDIKEHAF FGNFNWDKLA
810 820 830 840
GRLLEPPLVS KGETYAEDID IKQIEEEDAL NEGEPLDGDD SWDVDF
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AAKM01000002 Genomic DNA Translation: EDL46881.1 |
RefSeqi | XP_001616608.1, XM_001616558.1 |
Genome annotation databases
EnsemblProtistsi | EDL46881; EDL46881; PVX_084705 |
GeneIDi | 5475916 |
KEGGi | pvx:PVX_084705 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AAKM01000002 Genomic DNA Translation: EDL46881.1 |
RefSeqi | XP_001616608.1, XM_001616558.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4RZ7 | X-ray | 2.35 | A | 1-846 | [»] | |
5DYL | X-ray | 2.40 | A | 1-846 | [»] | |
5DZC | X-ray | 2.30 | A | 1-846 | [»] | |
5EZR | X-ray | 2.50 | A | 1-846 | [»] | |
5F0A | X-ray | 2.60 | A | 1-846 | [»] | |
5FET | X-ray | 3.07 | A | 1-846 | [»] | |
AlphaFoldDBi | A5K0N4 | |||||
SMRi | A5K0N4 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 126793.A5K0N4 |
Genome annotation databases
EnsemblProtistsi | EDL46881; EDL46881; PVX_084705 |
GeneIDi | 5475916 |
KEGGi | pvx:PVX_084705 |
Phylogenomic databases
InParanoidi | A5K0N4 |
OMAi | SKNPDGH |
Family and domain databases
CDDi | cd00038, CAP_ED, 3 hits cd05572, STKc_cGK, 1 hit |
Gene3Di | 2.60.120.10, 4 hits |
InterProi | View protein in InterPro IPR000961, AGC-kinase_C IPR002374, cGMP_dep_kinase IPR018490, cNMP-bd-like IPR018488, cNMP-bd_CS IPR000595, cNMP-bd_dom IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR014710, RmlC-like_jellyroll IPR008271, Ser/Thr_kinase_AS IPR035014, STKc_cGK |
Pfami | View protein in Pfam PF00027, cNMP_binding, 3 hits PF00069, Pkinase, 1 hit |
PIRSFi | PIRSF000559, cGMP-dep_kinase, 1 hit |
SMARTi | View protein in SMART SM00100, cNMP, 4 hits SM00220, S_TKc, 1 hit |
SUPFAMi | SSF51206, SSF51206, 4 hits SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS51285, AGC_KINASE_CTER, 1 hit PS00888, CNMP_BINDING_1, 3 hits PS00889, CNMP_BINDING_2, 3 hits PS50042, CNMP_BINDING_3, 4 hits PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | KGP_PLAVS | |
Accessioni | A5K0N4Primary (citable) accession number: A5K0N4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 10, 2021 |
Last sequence update: | July 10, 2007 | |
Last modified: | May 25, 2022 | |
This is version 101 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families