UniProtKB - A5IU64 (DAGK_STAA9)
Protein
Diacylglycerol kinase
Gene
dagK
Organism
Staphylococcus aureus (strain JH9)
Status
Functioni
Catalyzes the phosphorylation of diacylglycerol (DAG) into phosphatidic acid. Is a key enzyme involved in the production of lipoteichoic acid by reintroducing DAG formed from the breakdown of membrane phospholipids into the phosphatidylglycerol biosynthetic pathway.By similarity
Catalytic activityi
- EC:2.7.1.107By similarity
Cofactori
Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit. This ion appears to have a structural role and is required for catalytic activity.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 41 | ATPPROSITE-ProRule annotation | 1 | |
Binding sitei | 94 | ATPPROSITE-ProRule annotation | 1 | |
Metal bindingi | 213 | Magnesium; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 216 | MagnesiumBy similarity | 1 | |
Metal bindingi | 218 | Magnesium; via carbonyl oxygenBy similarity | 1 | |
Active sitei | 273 | Proton acceptorBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 10 – 14 | ATPPROSITE-ProRule annotation | 5 | |
Nucleotide bindingi | 67 – 73 | ATPPROSITE-ProRule annotation | 7 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- diacylglycerol kinase activity Source: UniProtKB-EC
- metal ion binding Source: UniProtKB-KW
- NAD+ kinase activity Source: InterPro
GO - Biological processi
- phospholipid biosynthetic process Source: UniProtKB-KW
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Diacylglycerol kinase (EC:2.7.1.107By similarity)Short name: DAG kinase Short name: DAGK |
Gene namesi | Name:dagK Ordered Locus Names:SaurJH9_1952 |
Organismi | Staphylococcus aureus (strain JH9) |
Taxonomic identifieri | 359786 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus › |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000386489 | 1 – 315 | Diacylglycerol kinaseAdd BLAST | 315 |
Interactioni
Subunit structurei
Homodimer.
By similarityFamily & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 132 | DAGKcPROSITE-ProRule annotationAdd BLAST | 132 |
Sequence similaritiesi
Belongs to the diacylglycerol/lipid kinase family.Curated
Phylogenomic databases
HOGENOMi | CLU_045532_1_0_9 |
OMAi | GFDSRVN |
Family and domain databases
Gene3Di | 3.40.50.10330, 1 hit |
InterProi | View protein in InterPro IPR017438, ATP-NAD_kinase_N IPR005218, Diacylglycerol/lipid_kinase IPR001206, Diacylglycerol_kinase_cat_dom IPR016064, NAD/diacylglycerol_kinase_sf |
Pfami | View protein in Pfam PF00781, DAGK_cat, 1 hit |
SMARTi | View protein in SMART SM00046, DAGKc, 1 hit |
SUPFAMi | SSF111331, SSF111331, 1 hit |
TIGRFAMsi | TIGR00147, TIGR00147, 1 hit |
PROSITEi | View protein in PROSITE PS50146, DAGK, 1 hit |
i Sequence
Sequence statusi: Complete.
A5IU64-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRKRARIIYN PTSGKELFKR ELPDALIKLE KAGYETSAYA TEKIGDATLE
60 70 80 90 100
AERAMHENYD VLIAAGGDGT LNEVVNGIAE KPNRPKLGVI PMGTVNDFGR
110 120 130 140 150
ALHIPNDIMG ALDVIIEGHS TKVDIGKMNN RYFINLAAGG QLTQVSYETP
160 170 180 190 200
SKLKSIVGPF AYYIKGFEML PQMKAVDLRI EYDGNVFQGE ALLFFLGLTN
210 220 230 240 250
SMAGFEKLVP DAKLDDGYFT LIIVEKSNLA ELGHIMTLAS RGEHTKHPKV
260 270 280 290 300
IYEKAKAINI SSFTDLQLNV DGEYGGKLPA NFLNLERHID VFAPNDIVNE
310
ELINNDHVDD NLIEE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000703 Genomic DNA Translation: ABQ49737.1 |
RefSeqi | WP_001231451.1, NC_009487.1 |
Genome annotation databases
GeneIDi | 59700694 |
KEGGi | saj:SaurJH9_1952 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000703 Genomic DNA Translation: ABQ49737.1 |
RefSeqi | WP_001231451.1, NC_009487.1 |
3D structure databases
SMRi | A5IU64 |
ModBasei | Search... |
Genome annotation databases
GeneIDi | 59700694 |
KEGGi | saj:SaurJH9_1952 |
Phylogenomic databases
HOGENOMi | CLU_045532_1_0_9 |
OMAi | GFDSRVN |
Family and domain databases
Gene3Di | 3.40.50.10330, 1 hit |
InterProi | View protein in InterPro IPR017438, ATP-NAD_kinase_N IPR005218, Diacylglycerol/lipid_kinase IPR001206, Diacylglycerol_kinase_cat_dom IPR016064, NAD/diacylglycerol_kinase_sf |
Pfami | View protein in Pfam PF00781, DAGK_cat, 1 hit |
SMARTi | View protein in SMART SM00046, DAGKc, 1 hit |
SUPFAMi | SSF111331, SSF111331, 1 hit |
TIGRFAMsi | TIGR00147, TIGR00147, 1 hit |
PROSITEi | View protein in PROSITE PS50146, DAGK, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DAGK_STAA9 | |
Accessioni | A5IU64Primary (citable) accession number: A5IU64 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 13, 2009 |
Last sequence update: | June 26, 2007 | |
Last modified: | April 7, 2021 | |
This is version 79 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |