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Protein

L-lactate dehydrogenase

Gene

ldh

Organism
Clostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of lactate to pyruvate.UniRule annotation

Catalytic activityi

(S)-lactate + NAD+ = pyruvate + NADH.UniRule annotation

Pathwayi: pyruvate fermentation to lactate

This protein is involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. L-lactate dehydrogenase (ldh)
This subpathway is part of the pathway pyruvate fermentation to lactate, which is itself part of Fermentation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-lactate from pyruvate, the pathway pyruvate fermentation to lactate and in Fermentation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei18NAD; via amide nitrogenUniRule annotation1
Binding sitei39NADUniRule annotation1
Binding sitei44NADUniRule annotation1
Binding sitei69NADUniRule annotation1
Binding sitei86SubstrateUniRule annotation1
Binding sitei92SubstrateUniRule annotation1
Binding sitei105NADUniRule annotation1
Binding sitei147NADUniRule annotation1
Active sitei179Proton acceptorUniRule annotation1
Binding sitei234SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi83 – 84NADUniRule annotation2
Nucleotide bindingi122 – 124NADUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
LigandNAD

Enzyme and pathway databases

BioCyciCBOT441771:G1G97-1490-MONOMER
UniPathwayiUPA00554; UER00611

Names & Taxonomyi

Protein namesi
Recommended name:
L-lactate dehydrogenaseUniRule annotation (EC:1.1.1.27UniRule annotation)
Short name:
L-LDHUniRule annotation
Gene namesi
Name:ldhUniRule annotation
Ordered Locus Names:CBO1519, CLC_1552
OrganismiClostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A)
Taxonomic identifieri441771 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
Proteomesi
  • UP000001986 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000264991 – 318L-lactate dehydrogenaseAdd BLAST318

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei225PhosphotyrosineUniRule annotation1

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA5I1Z6
SMRiA5I1Z6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni124 – 127Substrate bindingUniRule annotation4
Regioni152 – 155Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. LDH family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000213793
KOiK00016
OMAiMDLMQTA

Family and domain databases

Gene3Di3.90.110.10, 1 hit
HAMAPiMF_00488 Lactate_dehydrog, 1 hit
InterProiView protein in InterPro
IPR001557 L-lactate/malate_DH
IPR011304 L-lactate_DH
IPR018177 L-lactate_DH_AS
IPR022383 Lactate/malate_DH_C
IPR001236 Lactate/malate_DH_N
IPR015955 Lactate_DH/Glyco_Ohase_4_C
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF02866 Ldh_1_C, 1 hit
PF00056 Ldh_1_N, 1 hit
PIRSFiPIRSF000102 Lac_mal_DH, 1 hit
PRINTSiPR00086 LLDHDRGNASE
SUPFAMiSSF51735 SSF51735, 1 hit
SSF56327 SSF56327, 1 hit
TIGRFAMsiTIGR01771 L-LDH-NAD, 1 hit
PROSITEiView protein in PROSITE
PS00064 L_LDH, 1 hit

Sequencei

Sequence statusi: Complete.

A5I1Z6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKKRNTTKI SVIGAGSVGA TTAYALMLSG VATEIVLVDV NKSKTEGEAM
60 70 80 90 100
DLSHGADFVK PVNILSGDYK DTEGSDIVVI TAGAAQKVGE TRLQLINKNI
110 120 130 140 150
NIFKSIIPQV VKYNKDAILL VVSNPVDVLS YVTYKLSGFP KERVIGSGTV
160 170 180 190 200
LDTSRLKHEI GKRYKIDPRN VNTYIMGEHG DSEIATWSVT NIQNIKIDEY
210 220 230 240 250
ANKENLEYND NFRKEVYENV KNAAYEVINR KGATFYAIAL AVTRIVKAIL
260 270 280 290 300
GDEKTILPVS TLVENYYGIK DVYLGMPCIV GGSGIEKALS IDLNKTEASK
310
LVKSAETLKN TLNNASCL
Length:318
Mass (Da):34,734
Last modified:June 26, 2007 - v1
Checksum:i5F81554D68FD8698
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000727 Genomic DNA Translation: ABS36418.1
AM412317 Genomic DNA Translation: CAL83060.1
RefSeqiWP_011949073.1, NC_009698.1
YP_001254029.1, NC_009495.1
YP_001387415.1, NC_009698.1

Genome annotation databases

EnsemblBacteriaiABS36418; ABS36418; CLC_1552
GeneIDi5187351
5399830
KEGGicbh:CLC_1552
cbo:CBO1519
PATRICifig|413999.7.peg.1496

Similar proteinsi

Entry informationi

Entry nameiLDH_CLOBH
AccessioniPrimary (citable) accession number: A5I1Z6
Secondary accession number(s): A7G3Q0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 26, 2007
Last modified: May 23, 2018
This is version 73 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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