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Protein

Sister chromatid cohesion protein SCC2

Gene

SCC2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Essential protein required for cell fate determination during embryogenesis (PubMed:19228337, PubMed:15266054, PubMed:28137757). Involved in sister chromatid cohesion during meiosis and mitosis (PubMed:19228337, PubMed:19533160). Forms a complex with SCC4, which is required for the association of the cohesin complex with chromosomes (PubMed:28137757). Plays a structural role in chromatin, especially in centromere organization, chromosomal axis formation, and distribution of the cohesin subunit SCC3 on chromosomes (PubMed:19228337).4 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri699 – 748PHD-typePROSITE-ProRule annotationAdd BLAST50

GO - Molecular functioni

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • centromere complex assembly Source: TAIR
  • embryo development ending in seed dormancy Source: UniProtKB
  • meiotic sister chromatid cohesion Source: TAIR
  • regulation of gene expression Source: InterPro
  • sister chromatid cohesion Source: TAIR

Keywordsi

Biological processCell cycle, Cell division, Chromosome partition, Meiosis, Mitosis
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-ATH-2470946 Cohesin Loading onto Chromatin

Names & Taxonomyi

Protein namesi
Recommended name:
Sister chromatid cohesion protein SCC21 Publication
Alternative name(s):
Protein EMBRYO DEFECTIVE 27731 Publication
Protein SISTER-CHROMATID COHESION 21 Publication
Short name:
AtSCC21 Publication
Gene namesi
Name:SCC21 Publication
Synonyms:EMB27731 Publication
Ordered Locus Names:At5g15540Imported
ORF Names:T20K14.150Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G15540
TAIRilocus:2180942 AT5G15540

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Nucleus

Pathology & Biotechi

Disruption phenotypei

Defective embryo arrested at preglobular/early globular stage with the formation of giant endosperm nuclei (PubMed:19228337, PubMed:15266054, PubMed:28137757). Suspensor overproliferation phenotype preceded by ectopic auxin maxima distribution (PubMed:28137757). Reduced sister chromatid cohesion (PubMed:19533160). In conditional RNAi plants, sterility, arising from several defects in meiotic chromosome organization (e.g. failure of homologous pairing, loss of sister-chromatid cohesion, mixed segregation of chromosomes and chromosome fragmentation) and leading to shrunken and inviable pollen grains, and degeneration of the embryo sac. In the meiocytes, aberrant distribution of the cohesin subunit SCC3 on chromosomes, and defects in chromosomal axis formation (PubMed:19228337).4 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004406501 – 1846Sister chromatid cohesion protein SCC2Add BLAST1846

Proteomic databases

PaxDbiA5HEI1
PRIDEiA5HEI1

PTM databases

iPTMnetiA5HEI1

Expressioni

Tissue specificityi

Expressed in leaves, inflorescence and siliques.1 Publication

Developmental stagei

Detected throughout the embryo, covering all stages of development from pre-globular to torpedo stages. Also detected in the suspensor and endosperm.1 Publication

Gene expression databases

ExpressionAtlasiA5HEI1 baseline and differential

Interactioni

Subunit structurei

Interacts with SCC4 to form the cohesin loading complex.1 Publication

Protein-protein interaction databases

STRINGi3702.AT5G15540.1

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati243 – 282HEAT 1Sequence analysisAdd BLAST40
Repeati298 – 335HEAT 2Sequence analysisAdd BLAST38
Repeati438 – 460HEAT 3Sequence analysisAdd BLAST23
Repeati461 – 499HEAT 4Sequence analysisAdd BLAST39
Repeati532 – 572HEAT 5Sequence analysisAdd BLAST41
Repeati613 – 631HEAT 6Sequence analysisAdd BLAST19
Repeati632 – 672HEAT 7Sequence analysisAdd BLAST41
Repeati781 – 818HEAT 8Sequence analysisAdd BLAST38
Repeati869 – 906HEAT 9Sequence analysisAdd BLAST38
Repeati945 – 982HEAT 10Sequence analysisAdd BLAST38
Repeati984 – 1021HEAT 11Sequence analysisAdd BLAST38
Repeati1053 – 1097HEAT 12Sequence analysisAdd BLAST45
Repeati1113 – 1152HEAT 13Sequence analysisAdd BLAST40
Repeati1174 – 1213HEAT 14Sequence analysisAdd BLAST40
Repeati1227 – 1267HEAT 15Sequence analysisAdd BLAST41
Repeati1277 – 1314HEAT 16Sequence analysisAdd BLAST38
Repeati1427 – 1464HEAT 17Sequence analysisAdd BLAST38
Repeati1475 – 1512HEAT 18Sequence analysisAdd BLAST38
Repeati1533 – 1575HEAT 19Sequence analysisAdd BLAST43

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1813 – 1821Asp-richPROSITE-ProRule annotation9

Sequence similaritiesi

Belongs to the SCC2/Nipped-B family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri699 – 748PHD-typePROSITE-ProRule annotationAdd BLAST50

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1020 Eukaryota
ENOG410XP32 LUCA
HOGENOMiHOG000276888
KOiK06672
OMAiKAMKCLT
OrthoDBiEOG093600IF

Family and domain databases

Gene3Di1.25.10.10, 1 hit
3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR011989 ARM-like
IPR016024 ARM-type_fold
IPR026003 Cohesin_HEAT
IPR024986 Nipped-B_C
IPR033031 SCC2/Nipped-B
IPR019786 Zinc_finger_PHD-type_CS
IPR011011 Znf_FYVE_PHD
IPR001965 Znf_PHD
IPR019787 Znf_PHD-finger
IPR013083 Znf_RING/FYVE/PHD
PANTHERiPTHR21704 PTHR21704, 1 hit
PfamiView protein in Pfam
PF12765 Cohesin_HEAT, 1 hit
PF12830 Nipped-B_C, 1 hit
SMARTiView protein in SMART
SM00249 PHD, 1 hit
SUPFAMiSSF48371 SSF48371, 4 hits
SSF57903 SSF57903, 1 hit
PROSITEiView protein in PROSITE
PS01359 ZF_PHD_1, 1 hit
PS50016 ZF_PHD_2, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: A5HEI1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSNPSSSGLG SSSGLTHFGI GLANTVQSEV TPYLPLPSLP IFCGAAEPGE
60 70 80 90 100
FKLFDEVGQG SGYRSLDRSE ILAQSSRIAN MLHETDVSYL DLRNEARAPD
110 120 130 140 150
CNSGEHFQLY DLVLRCNPGA FEYVTPGPTC DPLFTNEGPQ KIISEPSVPV
160 170 180 190 200
KMQRQTDTHL ARSIEPEPVK RVLRPNHVED HSWQHETLTN QSPKDVTAYD
210 220 230 240 250
SRPETITMNE LSASKKPKGK KKRKDDLSSV QPDPSVLQES IVQNFCEMLE
260 270 280 290 300
DFCGRAEVPG DDRDEAEWSS VPVDEVRVLI NELMTIRSKM LLHMVPVDIL
310 320 330 340 350
SRLLRTLDHQ IHRAEGLSIY SEHSDSDSVL LVLGALESIH ASLAVMANSD
360 370 380 390 400
MPKQLYKEEI IERILEFSRH QMMAVMSAYD PSYRTGSKPA ENLAFEGDDD
410 420 430 440 450
DDNPDHDMGS ASKRRRIVKN SKVKKASVNR ISGAVNTALQ KLCTILGLLK
460 470 480 490 500
DLLLVERLSD SCILQLLKTS ITTFLVENIQ ILQLKAISLI GGIYNSYSQH
510 520 530 540 550
RTYVIDEISQ LLWKLPSSKR ALRAYLLPDE EQRQIQMVTA LLIQLVHNST
560 570 580 590 600
SLPETSRQAA SGNSILETSV DVGYLTKCHE AATETCCLFW TRVLERFTSF
610 620 630 640 650
KGQDASEIKL IIENLVMDLL TALNLPEYPS VSPILEVLCV ILLHNAGLKS
660 670 680 690 700
KDVSARIMAI ELLGTIAARL KRDAVLCSKD RFWTLLESDS EISVDQVCTK
710 720 730 740 750
DCTFCLGKRA GNLLVCQICQ RRFHGDCLGL KELDISSRNW HCPLCVCKRQ
760 770 780 790 800
LLVLQSYCKT DTKGTGKLES EESIENPSMI TKTEVVQQML LNYLQDVGSA
810 820 830 840 850
DDVHTFICWF YLCLWYKDVP KSQNKFKYYI ARLKAKSIIR NSGATTSFLT
860 870 880 890 900
RDAIKQITLA LGMNSSFSRG FDKILNMLLA SLRENAPNIR AKALRAVSII
910 920 930 940 950
VEADPEVLCD KRVQLAVEGR FCDSAISVRE AALELVGRHI ASHPDVGIKY
960 970 980 990 1000
FEKVAERIKD TGVSVRKRAI KIIRDMCTSN PNFSEFTSAC AEILSRISDD
1010 1020 1030 1040 1050
ESSVQDLVCK TFYEFWFEEP PGHHTQFASD ASSIPLELEK KTKQMVGLLS
1060 1070 1080 1090 1100
RTPNQQLLVT IIKRALALDF FPQAAKAAGI NPVALASVRR RCELMCKCLL
1110 1120 1130 1140 1150
EKILQVEEMS REEGEVQVLP YVLVLHAFCL VDPGLCTPAS DPTKFVITLQ
1160 1170 1180 1190 1200
PYLKSQADSR TGAQLLESII FIIDSVLPLI RKLPLSVTED LEQDLKHMIV
1210 1220 1230 1240 1250
RHSFLTVVHA CVRCLCSVSK LAGKGVSIVE HLLQFFFKRL EAQGSDNTQI
1260 1270 1280 1290 1300
AGRSLFCLGL LIRHGNSLIS TSGGKNFNLS GCLNLFKRHL RTEDIALKVR
1310 1320 1330 1340 1350
SLQALGFILI ARPEYMLEED IGKIIETTLA DEANGRMKMQ ALQNMYEYLL
1360 1370 1380 1390 1400
DAEKQLGSEK ASDNTVNSVE QGGHNVPVAA GAGDTNICGG IVQLFWDKIL
1410 1420 1430 1440 1450
GRCLDFDDQI RQTSLKIVEV VLRQGLVHPI TCVPYLIALE TDPQEANQKL
1460 1470 1480 1490 1500
AHHLLMNMHE KYPAFFESRL GDGLQMSFIF MQSISQVTSE PNQSLQQKGS
1510 1520 1530 1540 1550
TNMLGKNDHA SSTLTQARLG VSRIYKLIRG NRVSRNKFMT SIVRKFDNPT
1560 1570 1580 1590 1600
WNGSVISFLK YCTETLALLP FTSPDEPLYL VYSINRVMQI RAGAVESNLK
1610 1620 1630 1640 1650
ALLHKDSAKT QHGNGAYQQD PIPGHMNMMD LNTRIQEEPR HWNSYGHATL
1660 1670 1680 1690 1700
IDLNGSVYQD SRDQFTSYQV HNGKADVHKM TSSDPPELST DDLQKIQVDC
1710 1720 1730 1740 1750
LAAIAIQLLL KLKRYLKVTY SLNDDRCQAY SPTEPLKPGD PLSRQSVAFD
1760 1770 1780 1790 1800
LSETRTDLPS TYQDLVQRYQ EFKNAMREDT VDFTIYSTNV KRKRPTPRKT
1810 1820 1830 1840
SRSAKKTVAY NEDDDDDDND DRGWHGGGGR GAARRLNYST RSSNRR
Length:1,846
Mass (Da):206,759
Last modified:June 12, 2007 - v1
Checksum:iE810C627DB8776A7
GO
Isoform 2 (identifier: A5HEI1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     122-126: EYVTP → DLS

Note: Derived from EST data. No experimental confirmation available.Imported
Show »
Length:1,844
Mass (Da):206,484
Checksum:i62A195ED636A2129
GO
Isoform 3 (identifier: A5HEI1-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     393-1692: Missing.

Note: No experimental confirmation available.Imported
Show »
Length:546
Mass (Da):61,354
Checksum:i0A19BD018424E7CC
GO

Sequence cautioni

The sequence CAC01753 differs from that shown. Reason: Erroneous gene model prediction.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_058980122 – 126EYVTP → DLS in isoform 2. 5
Alternative sequenceiVSP_058981393 – 1692Missing in isoform 3. Add BLAST1300

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF520004 mRNA Translation: ABQ12620.1
EU046617 mRNA Translation: ABS88747.1
AL391143 Genomic DNA Translation: CAC01753.1 Sequence problems.
CP002688 Genomic DNA Translation: AED92174.1
CP002688 Genomic DNA Translation: AED92175.1
PIRiT51532
RefSeqiNP_001190317.1, NM_001203388.1 [A5HEI1-2]
NP_197058.2, NM_121558.4 [A5HEI1-1]
UniGeneiAt.31788

Genome annotation databases

EnsemblPlantsiAT5G15540.1; AT5G15540.1; AT5G15540 [A5HEI1-1]
AT5G15540.2; AT5G15540.2; AT5G15540 [A5HEI1-2]
GeneIDi831407
GrameneiAT5G15540.1; AT5G15540.1; AT5G15540 [A5HEI1-1]
AT5G15540.2; AT5G15540.2; AT5G15540 [A5HEI1-2]
KEGGiath:AT5G15540

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSCC2_ARATH
AccessioniPrimary (citable) accession number: A5HEI1
Secondary accession number(s): A7UDC4, F4K9U3, Q9LF28
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 5, 2017
Last sequence update: June 12, 2007
Last modified: July 18, 2018
This is version 102 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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