UniProtKB - A5H0J2 (DUR1_LACKL)
Protein
Urea amidolyase
Gene
DUR1,2
Organism
Lachancea kluyveri (Yeast) (Saccharomyces kluyveri)
Status
Functioni
Involved in uracil catabolism. Hydrolysis of urea to ammonia and CO2.1 Publication
Catalytic activityi
Cofactori
biotinBy similarity
: urea degradation Pathwayi
This protein is involved in step 1 and 2 of the subpathway that synthesizes CO(2) and NH(3) from urea (allophanate route).Proteins known to be involved in the 2 steps of the subpathway in this organism are:
- Urea amidolyase (DUR1,2)
- Urea amidolyase (DUR1,2)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CO(2) and NH(3) from urea (allophanate route), the pathway urea degradation and in Nitrogen metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 740 | ATPBy similarity | 1 | |
Binding sitei | 823 | ATPBy similarity | 1 | |
Binding sitei | 858 | ATPBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 115 – 122 | ATPPROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- allophanate hydrolase activity Source: UniProtKB-EC
- ATP binding Source: UniProtKB-KW
- metal ion binding Source: InterPro
- urea carboxylase activity Source: UniProtKB-EC
GO - Biological processi
- urea catabolic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Hydrolase, Ligase, Multifunctional enzyme |
Ligand | ATP-binding, Biotin, Nucleotide-binding |
Enzyme and pathway databases
UniPathwayi | UPA00258;UER00371 UPA00258;UER00372 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:DUR1,2 Synonyms:PYD13,15, URC3,5 |
Organismi | Lachancea kluyveri (Yeast) (Saccharomyces kluyveri) |
Taxonomic identifieri | 4934 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Lachancea |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000367271 | 1 – 1830 | Urea amidolyaseAdd BLAST | 1830 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1796 | N6-biotinyllysinePROSITE-ProRule annotationBy similarity | 1 |
Proteomic databases
PRIDEi | A5H0J2 |
Interactioni
Subunit structurei
Monomer.
By similarityFamily & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 625 – 1068 | Biotin carboxylationAdd BLAST | 444 | |
Domaini | 744 – 941 | ATP-graspPROSITE-ProRule annotationAdd BLAST | 198 | |
Domaini | 1752 – 1830 | Biotinyl-bindingPROSITE-ProRule annotationAdd BLAST | 79 |
Sequence similaritiesi
Belongs to the DUR1,2 family.Curated
Family and domain databases
Gene3Di | 2.40.100.10, 2 hits 3.90.1300.10, 1 hit |
InterProi | View protein in InterPro IPR014085, Allophanate_hydrolase IPR023631, Amidase_dom IPR036928, AS_sf IPR011761, ATP-grasp IPR005481, BC-like_N IPR001882, Biotin_BS IPR011764, Biotin_carboxylation_dom IPR005482, Biotin_COase_C IPR000089, Biotin_lipoyl IPR005479, CbamoylP_synth_lsu-like_ATP-bd IPR003778, CT_A_B IPR003833, CT_C_D IPR029000, Cyclophilin-like_dom_sf IPR016185, PreATP-grasp_dom_sf IPR011054, Rudment_hybrid_motif IPR011053, Single_hybrid_motif IPR014084, Urea_COase |
Pfami | View protein in Pfam PF01425, Amidase, 1 hit PF02785, Biotin_carb_C, 1 hit PF00289, Biotin_carb_N, 1 hit PF00364, Biotin_lipoyl, 1 hit PF02786, CPSase_L_D2, 1 hit PF02626, CT_A_B, 1 hit PF02682, CT_C_D, 1 hit |
SMARTi | View protein in SMART SM00796, AHS1, 1 hit SM00797, AHS2, 1 hit SM00878, Biotin_carb_C, 1 hit |
SUPFAMi | SSF50891, SSF50891, 2 hits SSF51230, SSF51230, 1 hit SSF51246, SSF51246, 1 hit SSF52440, SSF52440, 1 hit SSF75304, SSF75304, 1 hit |
TIGRFAMsi | TIGR02713, allophanate_hyd, 1 hit TIGR00724, urea_amlyse_rel, 1 hit TIGR02712, urea_carbox, 1 hit |
PROSITEi | View protein in PROSITE PS50975, ATP_GRASP, 1 hit PS50979, BC, 1 hit PS00188, BIOTIN, 1 hit PS50968, BIOTINYL_LIPOYL, 1 hit PS00866, CPSASE_1, 1 hit PS00867, CPSASE_2, 1 hit |
i Sequence
Sequence statusi: Complete.
A5H0J2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSVDTLGWSA QDWIDFHGKS TPEHSYNTLL SLLKSQKSAP EDPAWISLIN
60 70 80 90 100
EANLAHQWKV LQSKANKQQL PLYGVPIAVK DNIDSKGSPT TAACPAFEYN
110 120 130 140 150
PSADSTVVAL LKDAGAIVIG KTNLDQFATG LVGTRSPYGK TPCVFSDKHV
160 170 180 190 200
SGGSSAGSAS AVGRGIVPIA LGTDTAGSGR VPAALNNLIG LKPTKGLFSC
210 220 230 240 250
SGVVPACKSL DCVSVFAMNL SDAERCFKVM AKPDLENDEY SRPLPSNPLQ
260 270 280 290 300
KYPKNVTIAI PKEVPWYGET ENPKLYAKAI ENLKVAGASI VTIDFEPLLA
310 320 330 340 350
LARCLYEGAW VAERYEATKD FFATNPPESS LDPTVTSIIK TATKYDAADS
360 370 380 390 400
FRYEYQRQGI LQKVDQTLKD IDVLCVPTCP LNPTFEEVAA EPVLVNSRQG
410 420 430 440 450
TWTNFVNLAD MAALAVPAGF RPDGLPQGVT LIGKKFTDFA LLELANRYFK
460 470 480 490 500
VAFPQGSRTF GKFIDRQVTT KDDELRGPDI SPEDSVKLAV VGAHLKGLPL
510 520 530 540 550
YWQLEKVNAT YLGSPKTSKN YKLYALPKTG PILKPGLRRV GEETGSQIQL
560 570 580 590 600
EVYSVPKENF GEFISMVPEP LGIGSVELES GEWVKSFICE EFGYTQKGTV
610 620 630 640 650
DITKYGGFKK YIDFLKQEEA KVKKPFETVL IANRGEIAVR IIKTLKKLNI
660 670 680 690 700
RSVAVYSDPD KYSQHVIDAD LGVALNGRTA AETYLDIDKI IKAAKDTNAQ
710 720 730 740 750
AIIPGYGFLS ENAEFADKCV EEGIVFVGPS GEAIRKLGLK HSAREIAEKA
760 770 780 790 800
GVPLVPGSGL VTSAKEAKEI ANKLEYPVMV KSTAGGGGIG LQKVDSENEI
810 820 830 840 850
ERVFETVQHQ GKAYFGDSGV FLERFVENAR HVEIQMMGDG YGKAIAIGER
860 870 880 890 900
DCSLQRRNQK IIEETPAPNL GETTRTKMRQ AAESLGSLLK YKCAGTVEFI
910 920 930 940 950
YDERRDEFYF LEVNARLQVE HPITEMVTGL DLVEWMLRIA ADDAPDFESA
960 970 980 990 1000
NIVVTGASIE ARLYAENPAK DFRPSPGLLT DVHFPEWARV DTWVSKGTTV
1010 1020 1030 1040 1050
SAEYDPTLAK IIVHGKDRND AIMKMNKALN ETVVYGCITN IDYLRSIASS
1060 1070 1080 1090 1100
EMFKTAKVAT KILDSYDYKP CAFEVTSPGA YTTVQDYPGR VGYWRIGVPP
1110 1120 1130 1140 1150
SGPMDAYSFR LANRIVGNHY KAPAIELTLN GPKILFHTET IIAISGGIAA
1160 1170 1180 1190 1200
CSLNDKPIEQ NKPIQVNRGD HLAIGKLSVG CRAYLAIRGG IDVPEYLGSR
1210 1220 1230 1240 1250
STFALGNMGG YNGRVLKLGD VLFLNQPELA SSSLPGPAYE PQAPPANLLP
1260 1270 1280 1290 1300
KISDDKEWTI GVTCGPHGSP DFFKPESVEE FFSEKWKVHY NSNRFGVRLI
1310 1320 1330 1340 1350
GPKPKWARKD GGEGGLHPSN AHDYVYSLGA INFTGDEPVI ITSDGPSLGG
1360 1370 1380 1390 1400
FVCQAVVPEA ELWKVGQVKP GDSIQFVPIS YQVARQLKES QDAAIETLED
1410 1420 1430 1440 1450
GKLQTLTSDL ILPTYEDPVL VQLPKKSNLS PKVTYRQAGD RYILVEYGEN
1460 1470 1480 1490 1500
QMDLNIAYRI NQLINLVGKH KTVGIVEMSQ GVRSVLIEYD GYKISQGALL
1510 1520 1530 1540 1550
DTLVAYESEI QFDKNWSIKS KIFKLPLAFE DSKTLECVTR YQETIRSKAP
1560 1570 1580 1590 1600
WLPNNVDFVA EVNDITHKDV ENMLYSARFL VLGLGDVFLG APCAVPLDPR
1610 1620 1630 1640 1650
HRFLGSKYNP SRTYTKNGVV GIGGMYMCIY AMDSPGGYQL VGRTIPIWDK
1660 1670 1680 1690 1700
LKLGSHSQEH PWLLTPFDQV EFYPVSEEEL DRFTEDCENG KFPVQVEESV
1710 1720 1730 1740 1750
FDHKNYLKWI NENIESITEF QKSQGGAKAD EFARLIQVAN QELESSTTNK
1760 1770 1780 1790 1800
SAVEEEYPED AEMVYSEYSG RFWKPMVSAG DTVTKGDGLV IVEAMKTEMV
1810 1820 1830
VPAKKSGKVL KIVHKNGDMV DAGDLVAVIQ
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DQ512718 Genomic DNA Translation: ABF58890.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DQ512718 Genomic DNA Translation: ABF58890.1 |
3D structure databases
SMRi | A5H0J2 |
ModBasei | Search... |
Proteomic databases
PRIDEi | A5H0J2 |
Enzyme and pathway databases
UniPathwayi | UPA00258;UER00371 UPA00258;UER00372 |
Family and domain databases
Gene3Di | 2.40.100.10, 2 hits 3.90.1300.10, 1 hit |
InterProi | View protein in InterPro IPR014085, Allophanate_hydrolase IPR023631, Amidase_dom IPR036928, AS_sf IPR011761, ATP-grasp IPR005481, BC-like_N IPR001882, Biotin_BS IPR011764, Biotin_carboxylation_dom IPR005482, Biotin_COase_C IPR000089, Biotin_lipoyl IPR005479, CbamoylP_synth_lsu-like_ATP-bd IPR003778, CT_A_B IPR003833, CT_C_D IPR029000, Cyclophilin-like_dom_sf IPR016185, PreATP-grasp_dom_sf IPR011054, Rudment_hybrid_motif IPR011053, Single_hybrid_motif IPR014084, Urea_COase |
Pfami | View protein in Pfam PF01425, Amidase, 1 hit PF02785, Biotin_carb_C, 1 hit PF00289, Biotin_carb_N, 1 hit PF00364, Biotin_lipoyl, 1 hit PF02786, CPSase_L_D2, 1 hit PF02626, CT_A_B, 1 hit PF02682, CT_C_D, 1 hit |
SMARTi | View protein in SMART SM00796, AHS1, 1 hit SM00797, AHS2, 1 hit SM00878, Biotin_carb_C, 1 hit |
SUPFAMi | SSF50891, SSF50891, 2 hits SSF51230, SSF51230, 1 hit SSF51246, SSF51246, 1 hit SSF52440, SSF52440, 1 hit SSF75304, SSF75304, 1 hit |
TIGRFAMsi | TIGR02713, allophanate_hyd, 1 hit TIGR00724, urea_amlyse_rel, 1 hit TIGR02712, urea_carbox, 1 hit |
PROSITEi | View protein in PROSITE PS50975, ATP_GRASP, 1 hit PS50979, BC, 1 hit PS00188, BIOTIN, 1 hit PS50968, BIOTINYL_LIPOYL, 1 hit PS00866, CPSASE_1, 1 hit PS00867, CPSASE_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DUR1_LACKL | |
Accessioni | A5H0J2Primary (citable) accession number: A5H0J2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 24, 2009 |
Last sequence update: | June 12, 2007 | |
Last modified: | April 22, 2020 | |
This is version 78 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Documents
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families