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Protein

Biotin synthase

Gene

bioB

Organism
Geobacter uraniireducens (strain Rf4) (Geobacter uraniumreducens)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
  • [2Fe-2S] clusterUniRule annotationNote: Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

Pathwayi: biotin biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes biotin from 7,8-diaminononanoate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. ATP-dependent dethiobiotin synthetase BioD (bioD)
  2. Biotin synthase (bioB)
This subpathway is part of the pathway biotin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes biotin from 7,8-diaminononanoate, the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi66Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi70Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi73Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi143Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi203Iron-sulfur 2 (2Fe-2S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processBiotin biosynthesis
Ligand2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciGURA351605:G1G8X-2314-MONOMER
UniPathwayiUPA00078; UER00162

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
Gene namesi
Name:bioBUniRule annotation
Ordered Locus Names:Gura_2242
OrganismiGeobacter uraniireducens (strain Rf4) (Geobacter uraniumreducens)
Taxonomic identifieri351605 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfuromonadalesGeobacteraceaeGeobacter
Proteomesi
  • UP000006695 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003814071 – 329Biotin synthaseAdd BLAST329

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi351605.Gura_2242

Structurei

3D structure databases

ProteinModelPortaliA5G3Q7
SMRiA5G3Q7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CZF Bacteria
COG0502 LUCA
HOGENOMiHOG000239958
KOiK01012
OMAiADRFCMG
OrthoDBiPOG091H01DF

Family and domain databases

Gene3Di3.20.20.701 hit
HAMAPiMF_01694 BioB, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR010722 BATS_dom
IPR034416 BATS_domain_containing
IPR002684 Biotin_synth/BioAB
IPR024177 Biotin_synthase
IPR006638 Elp3/MiaB/NifB
IPR007197 rSAM
PANTHERiPTHR22976 PTHR22976, 1 hit
PfamiView protein in Pfam
PF06968 BATS, 1 hit
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF001619 Biotin_synth, 1 hit
SFLDiSFLDG01060 BATS_domain_containing, 1 hit
SFLDG01278 biotin_synthase_like, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00876 BATS, 1 hit
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00433 bioB, 1 hit

Sequencei

Sequence statusi: Complete.

A5G3Q7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENTIEALTG RIINGEAITH AEALELSQME GSALFPLFCV ANRIKEHFVG
60 70 80 90 100
DKVYLCSIIN AKSGRCPENC SFCAQSAHHK TDAPVYSLVD EDKMVACAHE
110 120 130 140 150
AEKNGSSCYG IITSGTSINK GEELTRICRA VKRIRQETGI TPSCSLGIID
160 170 180 190 200
YETALALKDA GVETYHHNLE TARSFFPNVC TTHDYEEDVE TVRVARKAGM
210 220 230 240 250
KVCCGGIFGL GESIPQRVEM ALTLRELDVD SVPLNFLNPI PGTRLENAVN
260 270 280 290 300
ITPMECLQTI ALFRMILPSK KISVCGGREK NLRDLQSWIF FAGASGTMIG
310 320
NYLTTTGRAA EEDWQMLKDL NLSAANCCD
Length:329
Mass (Da):36,042
Last modified:June 12, 2007 - v1
Checksum:i0A2A262A58C9CE4F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000698 Genomic DNA Translation: ABQ26425.1
RefSeqiWP_011939121.1, NC_009483.1

Genome annotation databases

EnsemblBacteriaiABQ26425; ABQ26425; Gura_2242
KEGGigur:Gura_2242

Similar proteinsi

Entry informationi

Entry nameiBIOB_GEOUR
AccessioniPrimary (citable) accession number: A5G3Q7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: June 12, 2007
Last modified: February 28, 2018
This is version 70 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome