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Protein

Na(+)-translocating NADH-quinone reductase subunit F

Gene

nqrF

Organism
Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

NQR complex catalyzes the reduction of ubiquinone-1 to ubiquinol by two successive reactions, coupled with the transport of Na+ ions from the cytoplasm to the periplasm. The first step is catalyzed by NqrF, which accepts electrons from NADH and reduces ubiquinone-1 to ubisemiquinone by a one-electron transfer pathway.UniRule annotation

Catalytic activityi

NADH + ubiquinone + n Na+(In) = NAD+ + ubiquinol + n Na+(Out).UniRule annotation1 Publication

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi70Iron-sulfur (2Fe-2S)UniRule annotation1 Publication1
Metal bindingi76Iron-sulfur (2Fe-2S)UniRule annotation1 Publication1
Metal bindingi79Iron-sulfur (2Fe-2S)UniRule annotation1 Publication1
Metal bindingi111Iron-sulfur (2Fe-2S)UniRule annotation1 Publication1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processIon transport, Sodium transport, Transport
Ligand2Fe-2S, FAD, Flavoprotein, Iron, Iron-sulfur, Metal-binding, NAD, Sodium, Ubiquinone

Names & Taxonomyi

Protein namesi
Recommended name:
Na(+)-translocating NADH-quinone reductase subunit FUniRule annotation (EC:1.6.5.8UniRule annotation1 Publication)
Short name:
Na(+)-NQR subunit FUniRule annotation
Short name:
Na(+)-translocating NQR subunit FUniRule annotation
Alternative name(s):
NQR complex subunit FUniRule annotation
NQR-1 subunit FUniRule annotation
Gene namesi
Name:nqrF1 PublicationUniRule annotation
Ordered Locus Names:VC0395_A1879, VC395_2406
OrganismiVibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395)
Taxonomic identifieri345073 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000000249 Componenti: Chromosome 2

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei4 – 24HelicalUniRule annotationAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi70C → A: Loss of the 2Fe-2S center, but does not affect flavin content. Exhibits very low NADH:quinone oxidoreductase activity. 1 Publication1
Mutagenesisi76C → A: Loss of the 2Fe-2S center, but does not affect flavin content. Exhibits very low NADH:quinone oxidoreductase activity. 1 Publication1
Mutagenesisi79C → A: Loss of the 2Fe-2S center, but does not affect flavin content. Exhibits very low NADH:quinone oxidoreductase activity. 1 Publication1
Mutagenesisi111C → A: Loss of the 2Fe-2S center, but does not affect flavin content. Exhibits very low NADH:quinone oxidoreductase activity. 1 Publication1
Mutagenesisi210R → L: Decreases flavin content, but does not affect the 2Fe-2S center. Exhibits very low NADH:quinone oxidoreductase activity. 1 Publication1
Mutagenesisi212Y → L: Decreases flavin content, but does not affect the 2Fe-2S center. Exhibits very low NADH:quinone oxidoreductase activity. 1 Publication1
Mutagenesisi246S → A: Decreases flavin content, but does not affect the 2Fe-2S center. Exhibits very low NADH:quinone oxidoreductase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000805951 – 408Na(+)-translocating NADH-quinone reductase subunit FAdd BLAST408

Proteomic databases

PRIDEiA5F5Y4

Interactioni

Subunit structurei

Composed of six subunits; NqrA, NqrB, NqrC, NqrD, NqrE and NqrF.UniRule annotation2 Publications

Protein-protein interaction databases

STRINGi345073.VC0395_A1879

Structurei

Secondary structure

1408
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliA5F5Y4
SMRiA5F5Y4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini33 – 1272Fe-2S ferredoxin-typeUniRule annotationAdd BLAST95
Domaini130 – 270FAD-binding FR-typeUniRule annotationAdd BLAST141

Sequence similaritiesi

Belongs to the NqrF family.UniRule annotationCurated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105D26 Bacteria
COG2871 LUCA
HOGENOMiHOG000263661
KOiK00351
OMAiYWYGGRS
OrthoDBiPOG091H05VQ

Family and domain databases

Gene3Di3.10.20.30, 1 hit
3.40.50.80, 1 hit
HAMAPiMF_00430 NqrF, 1 hit
InterProiView protein in InterPro
IPR036010 2Fe-2S_ferredoxin-like_sf
IPR001041 2Fe-2S_ferredoxin-type
IPR012675 Beta-grasp_dom_sf
IPR017927 Fd_Rdtase_FAD-bd
IPR039261 FNR_nucleotide-bd
IPR010205 NqrF
IPR008333 OxRdtase_FAD-bd_dom
IPR001433 OxRdtase_FAD/NAD-bd
IPR017938 Riboflavin_synthase-like_b-brl
PfamiView protein in Pfam
PF00970 FAD_binding_6, 1 hit
PF00111 Fer2, 1 hit
PF00175 NAD_binding_1, 1 hit
PIRSFiPIRSF000044 Cis_Diol_DH_RD, 1 hit
SUPFAMiSSF52343 SSF52343, 1 hit
SSF54292 SSF54292, 1 hit
SSF63380 SSF63380, 1 hit
TIGRFAMsiTIGR01941 nqrF, 1 hit
PROSITEiView protein in PROSITE
PS51085 2FE2S_FER_2, 1 hit
PS51384 FAD_FR, 1 hit

Sequencei

Sequence statusi: Complete.

A5F5Y4-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSTIIFGVVM FTLIILALVL VILFAKSKLV PTGDITISIN GDPEKAIVTQ
60 70 80 90 100
PGGKLLTALA GAGVFVSSAC GGGGSCGQCR VKIKSGGGDI LPTELDHISK
110 120 130 140 150
GEAREGERLA CQVAVKADMD LELPEEIFGV KKWECTVISN DNKATFIKEL
160 170 180 190 200
KLAIPDGESV PFRAGGYIQI EAPAHHVKYA DFDVPEKYRG DWDKFNLFRY
210 220 230 240 250
ESKVDEPIIR AYSMANYPEE FGIIMLNVRI ATPPPNNPNV PPGQMSSYIW
260 270 280 290 300
SLKAGDKCTI SGPFGEFFAK DTDAEMVFIG GGAGMAPMRS HIFDQLKRLK
310 320 330 340 350
SKRKMSYWYG ARSKREMFYV EDFDGLAAEN DNFVWHCALS DPQPEDNWTG
360 370 380 390 400
YTGFIHNVLY ENYLKDHEAP EDCEYYMCGP PMMNAAVINM LKNLGVEEEN

ILLDDFGG
Length:408
Mass (Da):45,067
Last modified:June 12, 2007 - v1
Checksum:iF5665E9623CAAD7B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000627 Genomic DNA Translation: ABQ21559.1
CP001235 Genomic DNA Translation: ACP10396.1
RefSeqiWP_000103964.1, NC_012582.1

Genome annotation databases

EnsemblBacteriaiABQ21559; ABQ21559; VC0395_A1879
ACP10396; ACP10396; VC395_2406
KEGGivco:VC0395_A1879
vcr:VC395_2406
PATRICifig|345073.21.peg.2319

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000627 Genomic DNA Translation: ABQ21559.1
CP001235 Genomic DNA Translation: ACP10396.1
RefSeqiWP_000103964.1, NC_012582.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4UAJX-ray2.70A129-408[»]
ProteinModelPortaliA5F5Y4
SMRiA5F5Y4
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi345073.VC0395_A1879

Proteomic databases

PRIDEiA5F5Y4

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABQ21559; ABQ21559; VC0395_A1879
ACP10396; ACP10396; VC395_2406
KEGGivco:VC0395_A1879
vcr:VC395_2406
PATRICifig|345073.21.peg.2319

Phylogenomic databases

eggNOGiENOG4105D26 Bacteria
COG2871 LUCA
HOGENOMiHOG000263661
KOiK00351
OMAiYWYGGRS
OrthoDBiPOG091H05VQ

Family and domain databases

Gene3Di3.10.20.30, 1 hit
3.40.50.80, 1 hit
HAMAPiMF_00430 NqrF, 1 hit
InterProiView protein in InterPro
IPR036010 2Fe-2S_ferredoxin-like_sf
IPR001041 2Fe-2S_ferredoxin-type
IPR012675 Beta-grasp_dom_sf
IPR017927 Fd_Rdtase_FAD-bd
IPR039261 FNR_nucleotide-bd
IPR010205 NqrF
IPR008333 OxRdtase_FAD-bd_dom
IPR001433 OxRdtase_FAD/NAD-bd
IPR017938 Riboflavin_synthase-like_b-brl
PfamiView protein in Pfam
PF00970 FAD_binding_6, 1 hit
PF00111 Fer2, 1 hit
PF00175 NAD_binding_1, 1 hit
PIRSFiPIRSF000044 Cis_Diol_DH_RD, 1 hit
SUPFAMiSSF52343 SSF52343, 1 hit
SSF54292 SSF54292, 1 hit
SSF63380 SSF63380, 1 hit
TIGRFAMsiTIGR01941 nqrF, 1 hit
PROSITEiView protein in PROSITE
PS51085 2FE2S_FER_2, 1 hit
PS51384 FAD_FR, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNQRF_VIBC3
AccessioniPrimary (citable) accession number: A5F5Y4
Secondary accession number(s): C3M414
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: June 12, 2007
Last modified: September 12, 2018
This is version 84 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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