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Protein

Fatty acid oxidation complex subunit alpha

Gene

fadJ

Organism
Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of a hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities.UniRule annotation

Catalytic activityi

(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.UniRule annotation
(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.UniRule annotation
(S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA.UniRule annotation

Pathwayi: fatty acid beta-oxidation

This protein is involved in the pathway fatty acid beta-oxidation, which is part of Lipid metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway fatty acid beta-oxidation and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei119Important for catalytic activityUniRule annotation1
Sitei141Important for catalytic activityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase, Lyase, Multifunctional enzyme, Oxidoreductase
Biological processFatty acid metabolism, Lipid degradation, Lipid metabolism
LigandNAD

Enzyme and pathway databases

UniPathwayi
UPA00659

Names & Taxonomyi

Protein namesi
Recommended name:
Fatty acid oxidation complex subunit alphaUniRule annotation
Including the following 2 domains:
Enoyl-CoA hydratase/3-hydroxybutyryl-CoA epimeraseUniRule annotation (EC:4.2.1.17UniRule annotation, EC:5.1.2.3UniRule annotation)
3-hydroxyacyl-CoA dehydrogenaseUniRule annotation (EC:1.1.1.35UniRule annotation)
Gene namesi
Name:fadJUniRule annotation
Ordered Locus Names:VC0395_A0565, VC395_1061
OrganismiVibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395)
Taxonomic identifieri345073 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000000249 Componenti: Chromosome 2

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000736321 – 708Fatty acid oxidation complex subunit alphaAdd BLAST708

Interactioni

Subunit structurei

Heterotetramer of two alpha chains (FadJ) and two beta chains (FadI).UniRule annotation

Protein-protein interaction databases

STRINGi345073.VC0395_A0565

Structurei

3D structure databases

ProteinModelPortaliA5F2P2
SMRiA5F2P2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 191Enoyl-CoA hydrataseUniRule annotationAdd BLAST191
Regioni311 – 7083-hydroxyacyl-CoA dehydrogenaseUniRule annotationAdd BLAST398

Sequence similaritiesi

In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.UniRule annotation
In the central section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DYT Bacteria
COG1024 LUCA
COG1250 LUCA
HOGENOMiHOG000261346
KOiK01782
OMAiPFRYMDT
OrthoDBiPOG091H00UW

Family and domain databases

HAMAPiMF_01617 FadJ, 1 hit
InterProiView protein in InterPro
IPR006180 3-OHacyl-CoA_DH_CS
IPR006176 3-OHacyl-CoA_DH_NAD-bd
IPR006108 3HC_DH_C
IPR008927 6-PGluconate_DH-like_C_sf
IPR029045 ClpP/crotonase-like_dom_sf
IPR018376 Enoyl-CoA_hyd/isom_CS
IPR001753 Enoyl-CoA_hydra/iso
IPR012802 FadJ
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF00725 3HCDH, 2 hits
PF02737 3HCDH_N, 1 hit
PF00378 ECH_1, 1 hit
SUPFAMiSSF48179 SSF48179, 2 hits
SSF51735 SSF51735, 1 hit
SSF52096 SSF52096, 1 hit
TIGRFAMsiTIGR02440 FadJ, 1 hit
PROSITEiView protein in PROSITE
PS00067 3HCDH, 1 hit
PS00166 ENOYL_COA_HYDRATASE, 1 hit

Sequencei

Sequence statusi: Complete.

A5F2P2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDNNNAFQLS FDEQHYAWLA IDVPGEKMNT LQAAFAEEMQ AVFATLNEKR
60 70 80 90 100
GQIKGLIIHS LKPDNFIAGA DVRMLEACQS VHEAQALASQ GQQMFQQLAD
110 120 130 140 150
LPFPVVAAIH GPCLGGGLEL ALACDYRVCT EDEVTRLGLP EVMLGLLPGS
160 170 180 190 200
GGTQRLPRLI GLLPALDLIL TGKQLRAKKA KKLGVVDACV PHSVLLDVAK
210 220 230 240 250
RLLEEKGHKK RAQVTLPIKE KLLANTGLGR KLIFDQAAKK TQQKTRGNYP
260 270 280 290 300
AAQAILEVIQ YGLEKGMHAG LEYEAKRFAE LVMTRESKAL RSIFFATTEM
310 320 330 340 350
KKDLGADAKP APVAAVGVLG GGLMGAGISH VTVAKAKTSV RIKDVANDGV
360 370 380 390 400
LNALNYNYKL FDKQRQRKIL TKAQLQAQMS QLSGGTGFVG FDRCDVVIEA
410 420 430 440 450
VFEDLKLKQQ MVADIEANAK PTTIFATNTS SLPIHQIASQ AQRPQNIVGL
460 470 480 490 500
HYFSPVEKMP LVEVIPHATT SDETIATVVT LARKQGKTPI VVKDCAGFYV
510 520 530 540 550
NRILAPYMNE AAQVLMAGEP IEKLDAALLD FGFPVGPITL LDEVGVDIGA
560 570 580 590 600
KIMPILVKEL GPRFQGPDVF DVLLKDNRKG RKSGKGFYTY KGSKKKEVDK
610 620 630 640 650
SVYKLLKLTP ESKLNDKEIA MRCLLPMLNE AVRCLDEGII RSARDGDMGA
660 670 680 690 700
IFGIGFPPFL GGPFRYMDTL GLTKVVEMMN QHTEKYGERF APCDGLLTRA

GLGEKFYP
Length:708
Mass (Da):77,299
Last modified:June 12, 2007 - v1
Checksum:i77AD9F2EDDA527A4
GO

Sequence cautioni

The sequence ACP09073 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000627 Genomic DNA Translation: ABQ20084.1
CP001235 Genomic DNA Translation: ACP09073.1 Different initiation.
RefSeqiWP_000369797.1, NC_012582.1

Genome annotation databases

EnsemblBacteriaiABQ20084; ABQ20084; VC0395_A0565
ACP09073; ACP09073; VC395_1061
KEGGivco:VC0395_A0565
vcr:VC395_1061
PATRICifig|345073.21.peg.1029

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000627 Genomic DNA Translation: ABQ20084.1
CP001235 Genomic DNA Translation: ACP09073.1 Different initiation.
RefSeqiWP_000369797.1, NC_012582.1

3D structure databases

ProteinModelPortaliA5F2P2
SMRiA5F2P2
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi345073.VC0395_A0565

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABQ20084; ABQ20084; VC0395_A0565
ACP09073; ACP09073; VC395_1061
KEGGivco:VC0395_A0565
vcr:VC395_1061
PATRICifig|345073.21.peg.1029

Phylogenomic databases

eggNOGiENOG4105DYT Bacteria
COG1024 LUCA
COG1250 LUCA
HOGENOMiHOG000261346
KOiK01782
OMAiPFRYMDT
OrthoDBiPOG091H00UW

Enzyme and pathway databases

UniPathwayi
UPA00659

Family and domain databases

HAMAPiMF_01617 FadJ, 1 hit
InterProiView protein in InterPro
IPR006180 3-OHacyl-CoA_DH_CS
IPR006176 3-OHacyl-CoA_DH_NAD-bd
IPR006108 3HC_DH_C
IPR008927 6-PGluconate_DH-like_C_sf
IPR029045 ClpP/crotonase-like_dom_sf
IPR018376 Enoyl-CoA_hyd/isom_CS
IPR001753 Enoyl-CoA_hydra/iso
IPR012802 FadJ
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF00725 3HCDH, 2 hits
PF02737 3HCDH_N, 1 hit
PF00378 ECH_1, 1 hit
SUPFAMiSSF48179 SSF48179, 2 hits
SSF51735 SSF51735, 1 hit
SSF52096 SSF52096, 1 hit
TIGRFAMsiTIGR02440 FadJ, 1 hit
PROSITEiView protein in PROSITE
PS00067 3HCDH, 1 hit
PS00166 ENOYL_COA_HYDRATASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiFADJ_VIBC3
AccessioniPrimary (citable) accession number: A5F2P2
Secondary accession number(s): C3LZ57
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: June 12, 2007
Last modified: October 10, 2018
This is version 77 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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