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Entry version 64 (29 Sep 2021)
Sequence version 1 (12 Jun 2007)
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Protein

Na(+)/H(+) antiporter NhaD

Gene

nhaD

Organism
Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Na+/H+ antiporter that extrudes sodium in exchange for external protons. Can also transport lithium.

3 Publications

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>pH dependencei

Optimum pH is 8.0. Totally inactive at pH 9.0.1 Publication

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Biological processAntiport, Ion transport, Sodium transport, Transport
LigandSodium

Protein family/group databases

Transport Classification Database

More...
TCDBi
2.A.62.1.6, the nhad na(+):h(+) antiporter (nhad) family

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Na(+)/H(+) antiporter NhaD
Alternative name(s):
Sodium/proton antiporter NhaD
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:nhaD
Ordered Locus Names:VC0395_0225, VC395_A1039
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiVibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri345073 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000249 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 1

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular_location_section">'Subcellular location'</a> section describes the subcellular compartment where each non-membrane region of a membrane-spanning protein is found.<p><a href='/help/topo_dom' target='_top'>More...</a></p>Topological domaini1 – 33PeriplasmicSequence analysisAdd BLAST33
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular_location_section">'Subcellular location'</a> section describes the extent of a membrane-spanning region of the protein. It denotes the presence of both alpha-helical transmembrane regions and the membrane spanning regions of beta-barrel transmembrane proteins.<p><a href='/help/transmem' target='_top'>More...</a></p>Transmembranei34 – 51HelicalSequence analysisAdd BLAST18
Topological domaini52 – 60CytoplasmicSequence analysis9
Transmembranei61 – 78HelicalSequence analysisAdd BLAST18
Topological domaini79 – 94PeriplasmicSequence analysisAdd BLAST16
Transmembranei95 – 112HelicalSequence analysisAdd BLAST18
Topological domaini113 – 132CytoplasmicSequence analysisAdd BLAST20
Transmembranei133 – 150HelicalSequence analysisAdd BLAST18
Topological domaini151 – 154PeriplasmicSequence analysis4
Transmembranei155 – 171HelicalSequence analysisAdd BLAST17
Topological domaini172 – 181CytoplasmicSequence analysis10
Transmembranei182 – 206HelicalSequence analysisAdd BLAST25
Topological domaini207 – 220PeriplasmicSequence analysisAdd BLAST14
Transmembranei221 – 238HelicalSequence analysisAdd BLAST18
Topological domaini239 – 258CytoplasmicSequence analysisAdd BLAST20
Transmembranei259 – 277HelicalSequence analysisAdd BLAST19
Topological domaini278 – 281PeriplasmicSequence analysis4
Transmembranei282 – 299HelicalSequence analysisAdd BLAST18
Topological domaini300 – 344CytoplasmicSequence analysisAdd BLAST45
Transmembranei345 – 362HelicalSequence analysisAdd BLAST18
Topological domaini363 – 378PeriplasmicSequence analysisAdd BLAST16
Transmembranei379 – 403HelicalSequence analysisAdd BLAST25
Topological domaini404 – 413CytoplasmicSequence analysis10
Transmembranei414 – 438HelicalSequence analysisAdd BLAST25
Topological domaini439 – 454PeriplasmicSequence analysisAdd BLAST16
Transmembranei455 – 472HelicalSequence analysisAdd BLAST18
Topological domaini473 – 477CytoplasmicSequence analysis5

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology_and_biotech_section">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi93H → A: Shifts the pH optimum to more acidic pH. 1 Publication1
Mutagenesisi100E → A: No change in activity. Shifts the pH optimum to more alkaline pH. 1 Publication1
Mutagenesisi150S → A: Lack of activity. 1 Publication1
Mutagenesisi154D → G: Lack of activity. 1 Publication1
Mutagenesisi155N → A: Lack of activity. 1 Publication1
Mutagenesisi157T → A: Decrease in activity. 1 Publication1
Mutagenesisi189N → A: Lack of activity. 1 Publication1
Mutagenesisi199D → A: Lack of activity. 1 Publication1
Mutagenesisi201T → A: Lack of activity. 1 Publication1
Mutagenesisi202T → A: Lack of activity. 1 Publication1
Mutagenesisi210H → A: Shifts the pH optimum to more acidic pH. 1 Publication1
Mutagenesisi251E → A: No change in activity. Shifts the pH optimum to more alkaline pH. 1 Publication1
Mutagenesisi274H → A: No change in activity. 1 Publication1
Mutagenesisi278H → A: No change in activity. 1 Publication1
Mutagenesisi342E → A: No change in activity. Shifts the pH optimum to more alkaline pH. 1 Publication1
Mutagenesisi344D → A or N: Lack of activity. 1 Publication1
Mutagenesisi344D → E: Decrease in activity. 1 Publication1
Mutagenesisi345T → A: Lack of activity. 1 Publication1
Mutagenesisi389S → A: Lack of activity. 1 Publication1
Mutagenesisi390S → A: No change in activity. 1 Publication1
Mutagenesisi393D → A: No change in activity. Shifts the pH optimum to more alkaline pH. 1 Publication1
Mutagenesisi393D → E or N: Lack of activity. 1 Publication1
Mutagenesisi394N → G: Lack of activity. 1 Publication1
Mutagenesisi425S → A: Lack of activity. 1 Publication1
Mutagenesisi428S → A: Decrease in activity. 1 Publication1
Mutagenesisi431S → A: Lack of activity. 1 Publication1
Mutagenesisi450H → A: No change in activity. 1 Publication1
Mutagenesisi468H → A: No change in activity. 1 Publication1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00004236611 – 477Na(+)/H(+) antiporter NhaDAdd BLAST477

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

STRING: functional protein association networks

More...
STRINGi
345073.VC395_A1039

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
COG1055, Bacteria

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_029697_0_0_6

Identification of Orthologs from Complete Genome Data

More...
OMAi
DAHKGFS

Family and domain databases

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR004680, Cit_transptr-like_dom

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF03600, CitMHS, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

A5F120-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTGRIALLSL TLFSPLSLAS TPDGQALDFT HSTIGYAALL IFAIAYTLVM
60 70 80 90 100
LEEYLQLRKS KPVLLAAGLI WAMIGYVYQQ TGSTEVARQA LEHNLLEYAE
110 120 130 140 150
LLLFLLVAMT YISAMEERRL FDALKAWMIN RGFNFHTLFW ITGWLAFFIS
160 170 180 190 200
PIADNLTTAL LMCAVVMKVG GENPKFVSLA CINIVIAANA GGAFSPFGDI
210 220 230 240 250
TTLMVWQAGH VSFLEFMDLF LPSLANYLVP ALVMSLFVPH QTPSSIQEVV
260 270 280 290 300
ELKRGAKRIV VLFLFTILSA IGFHAFFHFP PVIGMMMGLA YLQFFGYFLR
310 320 330 340 350
KTLARSLAKK TAIAMAKNDE AALKRIGSVV PFDVFRSISH AEWDTLLFFY
360 370 380 390 400
GVVMCVGGLS LLGYLGLVSE ILYTEWNPIW ANVLVGLLSS VVDNIPVMFA
410 420 430 440 450
VLSMQPEMSL GNWLLVTLTA GVGGSLLSIG SAAGVALMGA AHGKYTFLSH
460 470
LKWTPVILLG YVVSIVLHLL LNHQSFT
Length:477
Mass (Da):52,378
Last modified:June 12, 2007 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i90644FAD3CCE1990
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AF331042 Genomic DNA Translation: AAG48354.2
CP000626 Genomic DNA Translation: ABQ19159.1
CP001236 Genomic DNA Translation: ACP11869.1

Protein sequence database of the Protein Information Resource

More...
PIRi
D82390

NCBI Reference Sequences

More...
RefSeqi
WP_000147723.1, NZ_JAACZH010000003.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
ABQ19159; ABQ19159; VC0395_0225

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
57742369

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
vco:VC0395_0225
vcr:VC395_A1039

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|345073.21.peg.3762

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF331042 Genomic DNA Translation: AAG48354.2
CP000626 Genomic DNA Translation: ABQ19159.1
CP001236 Genomic DNA Translation: ACP11869.1
PIRiD82390
RefSeqiWP_000147723.1, NZ_JAACZH010000003.1

3D structure databases

Database of comparative protein structure models

More...
ModBasei
Search...

SWISS-MODEL Interactive Workspace

More...
SWISS-MODEL-Workspacei
Submit a new modelling project...

Protein-protein interaction databases

STRINGi345073.VC395_A1039

Protein family/group databases

TCDBi2.A.62.1.6, the nhad na(+):h(+) antiporter (nhad) family

Protocols and materials databases

The DNASU plasmid repository

More...
DNASUi
2612475

Genome annotation databases

EnsemblBacteriaiABQ19159; ABQ19159; VC0395_0225
GeneIDi57742369
KEGGivco:VC0395_0225
vcr:VC395_A1039
PATRICifig|345073.21.peg.3762

Phylogenomic databases

eggNOGiCOG1055, Bacteria
HOGENOMiCLU_029697_0_0_6
OMAiDAHKGFS

Family and domain databases

InterProiView protein in InterPro
IPR004680, Cit_transptr-like_dom
PfamiView protein in Pfam
PF03600, CitMHS, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiNHAD_VIBC3
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: A5F120
Secondary accession number(s): Q7DCN4, Q9EYG4, Q9KKT5
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 16, 2013
Last sequence update: June 12, 2007
Last modified: September 29, 2021
This is version 64 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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