Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Small toxic protein TisB

Gene

tisB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Toxic component of a type I toxin-antitoxin (TA) system (PubMed:18761622) (Probable). Overexpression causes cessation of growth, induces stress-response, a number of membrane protein genes, and leads to cell death (PubMed:15620655, PubMed:18761622, PubMed:18710431). Inhibits ATP synthesis, ATP levels drop drastically quickly after induction (PubMed:18761622, PubMed:24513967). Part of the programmed response to DNA damage; damage leads to increased accumulation of the protein which slows or stops bacterial growth, probably allowing DNA repair before cells continue to grow (PubMed:15620655, PubMed:18761622).1 Publication4 Publications

Caution

Originally tisA and tisB were fused into one ORF by frameshift reconstruction. TisA is probably a pseudogene.Curated

GO - Molecular functioni

  • anion channel activity Source: EcoCyc

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoliWiki
  • dormancy process Source: EcoCyc
  • SOS response Source: EcoCyc

Keywordsi

Biological processToxin-antitoxin system

Enzyme and pathway databases

BioCyciEcoCyc:MONOMER0-1922

Protein family/group databases

TCDBi1.C.103.1.1 the pore-forming toxin, tisb (tisb) family

Names & Taxonomyi

Protein namesi
Recommended name:
Small toxic protein TisB
Alternative name(s):
LexA-regulated protein TisB
Gene namesi
Name:tisB
Synonyms:ysdB
Ordered Locus Names:b4618, JW3649
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14402 tisB

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei6 – 28HelicalSequence analysisAdd BLAST23

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Competition experiments between isogenic strains with or without the tisB/istR-1 region were performed. In the presence of DNA-damaging agents deletion strains were disadvantaged and were almost extinct by 4 days.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003118341 – 29Small toxic protein TisBAdd BLAST29

Proteomic databases

PaxDbiA5A627
PRIDEiA5A627

Expressioni

Inductioni

Part of the SOS-response regulon, controlled by LexA; repressed by LexA, induced by DNA damage (PubMed:10760155). Antisense sRNA IstR-1 inhibits toxicity by inhibiting translation of tisAB mRNA; subsequent RNase 3 cleavage leads to a truncated mRNA. IstR-1 sequesters a standby ribosome binding site in the tisAB mRNA; as the levels of IstR-1 decrease during the SOS response this site opens and ribosomes are able to bind to initiate translation further downstream (PubMed:15620655, PubMed:17499044).3 Publications

Interactioni

Protein-protein interaction databases

STRINGi511145.b4618

Family & Domainsi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

KOiK19148

Family and domain databases

InterProiView protein in InterPro
IPR025211 TisB_toxin
PfamiView protein in Pfam
PF13939 TisB_toxin, 1 hit
TIGRFAMsiTIGR04459 TisB_tox, 1 hit

Sequencei

Sequence statusi: Complete.

A5A627-1 [UniParc]FASTAAdd to basket
« Hide
        10         20 
MNLVDIAILI LKLIVAALQL LDAVLKYLK
Length:29
Mass (Da):3,222
Last modified:May 29, 2007 - v1
Checksum:i6F3B7FE3D9FEFC80
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: ABP93456.1
AP009048 Genomic DNA No translation available.
RefSeqiWP_001054909.1, NZ_LN832404.1
YP_001165331.1, NC_000913.3

Genome annotation databases

EnsemblBacteriaiABP93456; ABP93456; b4618
GeneIDi5061527
KEGGieco:b4618
PATRICifig|83333.103.peg.4606

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: ABP93456.1
AP009048 Genomic DNA No translation available.
RefSeqiWP_001054909.1, NZ_LN832404.1
YP_001165331.1, NC_000913.3

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi511145.b4618

Protein family/group databases

TCDBi1.C.103.1.1 the pore-forming toxin, tisb (tisb) family

Proteomic databases

PaxDbiA5A627
PRIDEiA5A627

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABP93456; ABP93456; b4618
GeneIDi5061527
KEGGieco:b4618
PATRICifig|83333.103.peg.4606

Organism-specific databases

EcoGeneiEG14402 tisB

Phylogenomic databases

KOiK19148

Enzyme and pathway databases

BioCyciEcoCyc:MONOMER0-1922

Miscellaneous databases

PROiPR:A5A627

Family and domain databases

InterProiView protein in InterPro
IPR025211 TisB_toxin
PfamiView protein in Pfam
PF13939 TisB_toxin, 1 hit
TIGRFAMsiTIGR04459 TisB_tox, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTISB_ECOLI
AccessioniPrimary (citable) accession number: A5A627
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: May 29, 2007
Last modified: September 12, 2018
This is version 52 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again