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Protein

Biotin synthase

Gene

bioB

Organism
Caldicellulosiruptor saccharolyticus (strain ATCC 43494 / DSM 8903 / Tp8T 6331)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
  • [2Fe-2S] clusterUniRule annotationNote: Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

Pathwayi: biotin biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes biotin from 7,8-diaminononanoate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. ATP-dependent dethiobiotin synthetase BioD (bioD)
  2. Biotin synthase (bioB)
This subpathway is part of the pathway biotin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes biotin from 7,8-diaminononanoate, the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi73Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi77Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi80Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi117Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi149Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi209Iron-sulfur 2 (2Fe-2S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processBiotin biosynthesis
Ligand2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciCSAC351627:G1G8P-325-MONOMER
UniPathwayiUPA00078; UER00162

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
Gene namesi
Name:bioBUniRule annotation
Ordered Locus Names:Csac_0307
OrganismiCaldicellulosiruptor saccharolyticus (strain ATCC 43494 / DSM 8903 / Tp8T 6331)
Taxonomic identifieri351627 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacterales Family III. Incertae SedisCaldicellulosiruptor
Proteomesi
  • UP000000256 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003812791 – 330Biotin synthaseAdd BLAST330

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi351627.Csac_0307

Structurei

3D structure databases

ProteinModelPortaliA4XGB8
SMRiA4XGB8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CZF Bacteria
COG0502 LUCA
HOGENOMiHOG000239958
KOiK01012
OMAiADRFCMG
OrthoDBiPOG091H01DF

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_01694 BioB, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR010722 BATS_dom
IPR034416 BATS_domain_containing
IPR002684 Biotin_synth/BioAB
IPR024177 Biotin_synthase
IPR006638 Elp3/MiaB/NifB
IPR007197 rSAM
PANTHERiPTHR22976 PTHR22976, 1 hit
PfamiView protein in Pfam
PF06968 BATS, 1 hit
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF001619 Biotin_synth, 1 hit
SFLDiSFLDG01060 BATS_domain_containing, 1 hit
SFLDG01278 biotin_synthase_like, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00876 BATS, 1 hit
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00433 bioB, 1 hit

Sequencei

Sequence statusi: Complete.

A4XGB8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLNFLQSVQF AKEIEKKIIE YEQDIDISEA TMLYEIAKHD FDLVKNLADT
60 70 80 90 100
IKRYYFKNNI ELCSIYPAKV GMCTEDCKFC SQSIYHSCKI EIKDVASVDE
110 120 130 140 150
VIEYLENIIA FPVKRFSLVT SGGELNDSEF ERIIDIISYI SKNYDIHLCA
160 170 180 190 200
SLGFLTKERA KKLMLAGVTR YHNNLETSST YFKNICSTHT QAQKIETLKI
210 220 230 240 250
AKETGLEICS GGIISMGEDM IERIKLAFEL RELKVDSVPI NILNPIKGTP
260 270 280 290 300
FEDMKIMDQN EIFITLALFR IVLPRKTILL AGGKENALKD MEKIAYKCGI
310 320 330
NGCMVGNYLT TQGMGIKEKV EMLESLGLEV
Length:330
Mass (Da):37,494
Last modified:May 29, 2007 - v1
Checksum:iA9E7096310F5FA68
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000679 Genomic DNA Translation: ABP65953.1
RefSeqiWP_011915916.1, NC_009437.1

Genome annotation databases

EnsemblBacteriaiABP65953; ABP65953; Csac_0307
KEGGicsc:Csac_0307

Similar proteinsi

Entry informationi

Entry nameiBIOB_CALS8
AccessioniPrimary (citable) accession number: A4XGB8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: May 29, 2007
Last modified: May 23, 2018
This is version 68 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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