Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Acetyl-coenzyme A synthetase

Gene

acsA

Organism
Pyrobaculum arsenaticum (strain DSM 13514 / JCM 11321)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA.UniRule annotation

Catalytic activityi

ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei326Coenzyme AUniRule annotation1
Binding sitei517ATPUniRule annotation1
Binding sitei532ATPUniRule annotation1
Binding sitei543ATPUniRule annotation1
Metal bindingi554Magnesium; via carbonyl oxygenUniRule annotation1
Metal bindingi556Magnesium; via carbonyl oxygenUniRule annotation1
Metal bindingi559Magnesium; via carbonyl oxygenUniRule annotation1
Binding sitei601Coenzyme AUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi402 – 404ATPUniRule annotation3
Nucleotide bindingi426 – 431ATPUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Acetyl-coenzyme A synthetaseUniRule annotation (EC:6.2.1.1UniRule annotation)
Short name:
AcCoA synthetaseUniRule annotation
Short name:
AcsUniRule annotation
Alternative name(s):
Acetate--CoA ligaseUniRule annotation
Acyl-activating enzymeUniRule annotation
Gene namesi
Name:acsAUniRule annotation
Ordered Locus Names:Pars_0948
OrganismiPyrobaculum arsenaticum (strain DSM 13514 / JCM 11321)
Taxonomic identifieri340102 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiThermoprotealesThermoproteaceaePyrobaculum
Proteomesi
  • UP000001567 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000653071 – 670Acetyl-coenzyme A synthetaseAdd BLAST670

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei626N6-acetyllysineUniRule annotation1

Post-translational modificationi

Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme.UniRule annotation

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiA4WJG1

Structurei

3D structure databases

ProteinModelPortaliA4WJG1
SMRiA4WJG1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni205 – 208Coenzyme A bindingUniRule annotation4

Sequence similaritiesi

Belongs to the ATP-dependent AMP-binding enzyme family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000229981
KOiK01895
OMAiDHWWHDL
OrthoDBiPOG093Z0181

Family and domain databases

CDDicd05966 ACS, 1 hit
HAMAPiMF_01123 Ac_CoA_synth, 1 hit
InterProiView protein in InterPro
IPR011904 Ac_CoA_lig
IPR032387 ACAS_N
IPR025110 AMP-bd_C
IPR020845 AMP-binding_CS
IPR000873 AMP-dep_Synth/Lig
PfamiView protein in Pfam
PF16177 ACAS_N, 1 hit
PF00501 AMP-binding, 1 hit
PF13193 AMP-binding_C, 1 hit
TIGRFAMsiTIGR02188 Ac_CoA_lig_AcsA, 1 hit
PROSITEiView protein in PROSITE
PS00455 AMP_BINDING, 1 hit

Sequencei

Sequence statusi: Complete.

A4WJG1-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSLKSLEKET ELPFDEYVLN DKWRHKYTPI DTYFKFHQQA IENLESFWES
60 70 80 90 100
VAKELEWFKP WDKVLDASNP PFYKWFVGGR LNLSYLAVDR HVKTWRKNKL
110 120 130 140 150
AIEWEGEPLD ESGYPIDRRK LTYYDLFREV NRVACMLKCN FGVKKGDRVT
160 170 180 190 200
LYLPMVPELP ITMLAAWRIG AITNVVFSGF SADALAERIN DSQSRIVITA
210 220 230 240 250
DGFWRRGKVV RLKEVVDSAL EKAPGVENVI VLPRLGLKDV PMTEGRDFWW
260 270 280 290 300
NRLMQGIPPN TYIEPEPVES EHPSFILYTS GTTGKPKGIV HDTGGWAVHV
310 320 330 340 350
YATMKWVFDI RDDDIYWCTA DIGWVTGHSY VVLGPLLMGT TEIIYEGAPD
360 370 380 390 400
YPQPDRWWSI IERYGVTILY TSPTAIRMFM RYGEAWPGKH DLSTLRIIHS
410 420 430 440 450
VGEPINPEAW RWAYRVLGNE KVAFGSTWWM TETGGIVISH APGLYLVPMK
460 470 480 490 500
PGTNGPPLPG FEVDVVDENG NPVPPGVKGY LVIKKPWPGM LHGIWGDPER
510 520 530 540 550
YVKTYWSRFP GMFYVGDYAI KDKDGYIWVL GRADEVIKVA GHRLGTYELE
560 570 580 590 600
SAFVAHPAVA EAAVVGVPDA IKGEVPIAFV VLKQGVTPTE ELRKELREHI
610 620 630 640 650
RKSIGPIAEP AQIFFVTKLP KTRSGKIMRR LLKAVATGAP LGDVTTLEDE
660 670
TSVEEAKKAY EELKAEMAGP
Length:670
Mass (Da):75,997
Last modified:May 29, 2007 - v1
Checksum:iEAB6B22635B6CF63
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000660 Genomic DNA Translation: ABP50528.1

Genome annotation databases

EnsemblBacteriaiABP50528; ABP50528; Pars_0948
KEGGipas:Pars_0948

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000660 Genomic DNA Translation: ABP50528.1

3D structure databases

ProteinModelPortaliA4WJG1
SMRiA4WJG1
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiA4WJG1

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABP50528; ABP50528; Pars_0948
KEGGipas:Pars_0948

Phylogenomic databases

HOGENOMiHOG000229981
KOiK01895
OMAiDHWWHDL
OrthoDBiPOG093Z0181

Family and domain databases

CDDicd05966 ACS, 1 hit
HAMAPiMF_01123 Ac_CoA_synth, 1 hit
InterProiView protein in InterPro
IPR011904 Ac_CoA_lig
IPR032387 ACAS_N
IPR025110 AMP-bd_C
IPR020845 AMP-binding_CS
IPR000873 AMP-dep_Synth/Lig
PfamiView protein in Pfam
PF16177 ACAS_N, 1 hit
PF00501 AMP-binding, 1 hit
PF13193 AMP-binding_C, 1 hit
TIGRFAMsiTIGR02188 Ac_CoA_lig_AcsA, 1 hit
PROSITEiView protein in PROSITE
PS00455 AMP_BINDING, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiACSA_PYRAR
AccessioniPrimary (citable) accession number: A4WJG1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: May 29, 2007
Last modified: October 10, 2018
This is version 65 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again