UniProtKB - A4UVI1 (FADS1_PAPAN)
Acyl-CoA (8-3)-desaturase
FADS1
Functioni
Acts as a front-end fatty acyl-coenzyme A (CoA) desaturase that introduces a cis double bond at carbon 5 located between a preexisting double bond and the carboxyl end of the fatty acyl chain. Involved in biosynthesis of highly unsaturated fatty acids (HUFA) from the essential polyunsaturated fatty acids (PUFA) linoleic acid (LA) (18:2n-6) and alpha-linolenic acid (ALA) (18:3n-3) precursors. Specifically, desaturates dihomo-gamma-linoleoate (DGLA) (20:3n-6) and eicosatetraenoate (ETA) (20:4n-3) to generate arachidonate (AA) (20:4n-6) and eicosapentaenoate (EPA) (20:5n-3), respectively (Probable). As a rate limiting enzyme for DGLA (20:3n-6) and AA (20:4n-6)-derived eicosanoid biosynthesis, controls the metabolism of inflammatory lipids like prostaglandin E2, critical for efficient acute inflammatory response and maintenance of epithelium homeostasis. Contributes to membrane phospholipid biosynthesis by providing AA (20:4n-6) as a major acyl chain esterified into phospholipids. In particular, regulates phosphatidylinositol-4,5-bisphosphate levels, modulating inflammatory cytokine production in T-cells (By similarity).
Also desaturates (11E)-octadecenoate (trans-vaccenoate)(18:1n-9), a metabolite in the biohydrogenation pathway of LA (18:2n-6) (By similarity).
By similarity1 PublicationDoes not exhibit any catalytic activity toward 20:3n-6, but it may enhance FADS2 activity.
1 PublicationCatalytic activityi
- (8Z,11Z,14Z)-eicosatrienoyl-CoA + 2 Fe(II)-[cytochrome b5] + 2 H+ + O2 = (5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + 2 Fe(III)-[cytochrome b5] + 2 H2O1 PublicationEC:1.14.19.441 PublicationThis reaction proceeds in the forward1 Publication direction.
- (8Z,11Z,14Z,17Z)-eicosatetraenoyl-CoA + 2 Fe(II)-[cytochrome b5] + 2 H+ + O2 = (5Z,8Z,11Z,14Z,17Z)-eicosapentaenoyl-CoA + 2 Fe(III)-[cytochrome b5] + 2 H2OBy similarity1 PublicationEC:1.14.19.44By similarity1 PublicationThis reaction proceeds in the forward1 Publication direction.
- (11E)-octadecenoyl-CoA + 2 Fe(II)-[cytochrome b5] + 2 H+ + O2 = (5Z,11E)-octadecadienoyl-CoA + 2 Fe(III)-[cytochrome b5] + 2 H2OBy similarityThis reaction proceeds in the forwardBy similarity direction.
: polyunsaturated fatty acid biosynthesis Pathwayi
This protein is involved in the pathway polyunsaturated fatty acid biosynthesis, which is part of Lipid metabolism.View all proteins of this organism that are known to be involved in the pathway polyunsaturated fatty acid biosynthesis and in Lipid metabolism.
GO - Molecular functioni
- acyl-CoA delta5-desaturase activity Source: UniProtKB
GO - Biological processi
- unsaturated fatty acid biosynthetic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Electron transport, Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Transport |
Enzyme and pathway databases
UniPathwayi | UPA00658 |
Names & Taxonomyi
Protein namesi | Recommended name: Acyl-CoA (8-3)-desaturaseCurated (EC:1.14.19.441 Publication)Alternative name(s): Delta(5) fatty acid desaturase Short name: D5DBy similarity Short name: Delta(5) desaturaseBy similarity Short name: Delta-5 desaturaseBy similarity Fatty acid desaturase 1By similarity |
Gene namesi | Name:FADS1By similarity |
Organismi | Papio anubis (Olive baboon) |
Taxonomic identifieri | 9555 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Papio |
Proteomesi |
|
Subcellular locationi
Mitochondrion
- Mitochondrion 1 Publication
Endoplasmic reticulum
- Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein 1 Publication
Endoplasmic reticulum
- Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein 1 Publication
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB
Mitochondrion
- mitochondrion Source: UniProtKB
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 121 | CytoplasmicSequence analysisAdd BLAST | 121 | |
Transmembranei | 122 – 142 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 143 – 146 | LumenalSequence analysis | 4 | |
Transmembranei | 147 – 167 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 168 – 267 | CytoplasmicSequence analysisAdd BLAST | 100 | |
Transmembranei | 268 – 288 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 289 – 305 | LumenalSequence analysisAdd BLAST | 17 | |
Transmembranei | 306 – 326 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 327 – 444 | CytoplasmicSequence analysisAdd BLAST | 118 |
Keywords - Cellular componenti
Endoplasmic reticulum, Membrane, MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000307098 | 1 – 444 | Acyl-CoA (8-3)-desaturaseAdd BLAST | 444 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 |
Keywords - PTMi
AcetylationExpressioni
Tissue specificityi
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 17 – 94 | Cytochrome b5 heme-bindingPROSITE-ProRule annotationAdd BLAST | 78 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 179 – 183 | Histidine box-1 | 5 | |
Motifi | 216 – 220 | Histidine box-2 | 5 | |
Motifi | 382 – 386 | Histidine box-3 | 5 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG4232, Eukaryota |
OrthoDBi | 1060606at2759 |
Family and domain databases
Gene3Di | 3.10.120.10, 1 hit |
InterProi | View protein in InterPro IPR001199, Cyt_B5-like_heme/steroid-bd IPR036400, Cyt_B5-like_heme/steroid_sf IPR005804, FA_desaturase_dom IPR012171, Fatty_acid_desaturase |
PANTHERi | PTHR19353, PTHR19353, 1 hit |
Pfami | View protein in Pfam PF00173, Cyt-b5, 1 hit PF00487, FA_desaturase, 1 hit |
PIRSFi | PIRSF015921, FA_sphinglp_des, 1 hit |
PRINTSi | PR00363, CYTOCHROMEB5 |
SMARTi | View protein in SMART SM01117, Cyt-b5, 1 hit |
SUPFAMi | SSF55856, SSF55856, 1 hit |
PROSITEi | View protein in PROSITE PS50255, CYTOCHROME_B5_2, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAPDPVAAKT PVQGPTPRYF TWDEVAQRSG CEERWLVIDR KVYDISEFTR
60 70 80 90 100
RHPGGSRVIS HYAGQDATDP FVAFHSNKGL VKKYMNSLLI GELSPEQPSF
110 120 130 140 150
EPTKNKELTD EFRELRATVE QMGLMKANHV FFLLYLLHIL LLDGAAWLTL
160 170 180 190 200
WIFGTSFLPF LLCAVLLTAA QIQAGWLQHD LGHLSVFSTS KWNHLVHHFV
210 220 230 240 250
IGHLKGVPAS WWNHMHFQHH AKPNCFGKDP DINMHPFFFA LGKILSVELG
260 270 280 290 300
KQKKKYMPYN HQHKYFFLIG PPALVPFFFQ WYVFYFVIQR KKWVDLAWMI
310 320 330 340 350
TFYIRLLLTY VPLLGLKAFL GLYFIVRFLE SNWFVWVTQM NHIPMHIDHD
360 370 380 390 400
RNMDWVSTQL QATCNVHKSA FNDWFSGHLN FQIEHHLFPM MPRHNYHKVA
410 420 430 440
PLVQSLCAKH GIEYQSKPLL SAFADIIHSL KESGQLWLDA YLHQ
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 425 | D → N in AFC78250 (PubMed:22619218).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_053444 | 1 – 84 | Missing in isoform 2. 1 PublicationAdd BLAST | 84 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DQ779578 mRNA Translation: ABG77281.1 EF531577 mRNA Translation: ABP06289.1 HQ440212 mRNA Translation: ADQ89961.1 JF518968 mRNA Translation: AFC78250.1 JF518969 mRNA Translation: AFC78251.1 JF518970 mRNA Translation: AFC78252.1 JF518971 mRNA Translation: AFC78253.1 JF518972 mRNA Translation: AFC78254.1 JF518974 mRNA Translation: AFC78256.1 |
RefSeqi | NP_001106097.1, NM_001112627.1 [A4UVI1-1] |
Genome annotation databases
GeneIDi | 100126707 |
KEGGi | panu:100126707 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DQ779578 mRNA Translation: ABG77281.1 EF531577 mRNA Translation: ABP06289.1 HQ440212 mRNA Translation: ADQ89961.1 JF518968 mRNA Translation: AFC78250.1 JF518969 mRNA Translation: AFC78251.1 JF518970 mRNA Translation: AFC78252.1 JF518971 mRNA Translation: AFC78253.1 JF518972 mRNA Translation: AFC78254.1 JF518974 mRNA Translation: AFC78256.1 |
RefSeqi | NP_001106097.1, NM_001112627.1 [A4UVI1-1] |
3D structure databases
AlphaFoldDBi | A4UVI1 |
SMRi | A4UVI1 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9555.ENSPANP00000011222 |
Genome annotation databases
GeneIDi | 100126707 |
KEGGi | panu:100126707 |
Organism-specific databases
CTDi | 3992 |
Phylogenomic databases
eggNOGi | KOG4232, Eukaryota |
OrthoDBi | 1060606at2759 |
Enzyme and pathway databases
UniPathwayi | UPA00658 |
Family and domain databases
Gene3Di | 3.10.120.10, 1 hit |
InterProi | View protein in InterPro IPR001199, Cyt_B5-like_heme/steroid-bd IPR036400, Cyt_B5-like_heme/steroid_sf IPR005804, FA_desaturase_dom IPR012171, Fatty_acid_desaturase |
PANTHERi | PTHR19353, PTHR19353, 1 hit |
Pfami | View protein in Pfam PF00173, Cyt-b5, 1 hit PF00487, FA_desaturase, 1 hit |
PIRSFi | PIRSF015921, FA_sphinglp_des, 1 hit |
PRINTSi | PR00363, CYTOCHROMEB5 |
SMARTi | View protein in SMART SM01117, Cyt-b5, 1 hit |
SUPFAMi | SSF55856, SSF55856, 1 hit |
PROSITEi | View protein in PROSITE PS50255, CYTOCHROME_B5_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | FADS1_PAPAN | |
Accessioni | A4UVI1Primary (citable) accession number: A4UVI1 Secondary accession number(s): F1CNE5 Q0PR60 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 2, 2007 |
Last sequence update: | May 15, 2007 | |
Last modified: | May 25, 2022 | |
This is version 69 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families