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Protein

Acetate kinase

Gene

ackA

Organism
Mycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 / PYR-GCK))
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of acetyl phosphate from acetate and ATP. Can also catalyze the reverse reaction.UniRule annotation

Catalytic activityi

ATP + acetate = ADP + acetyl phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotationNote: Mg2+. Can also accept Mn2+.UniRule annotation

Pathwayi: acetyl-CoA biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes acetyl-CoA from acetate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Acetate kinase (ackA)
  2. Phosphate acetyltransferase (Mflv_0205)
This subpathway is part of the pathway acetyl-CoA biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes acetyl-CoA from acetate, the pathway acetyl-CoA biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi9MagnesiumUniRule annotation1
Binding sitei16ATPUniRule annotation1
Binding sitei74SubstrateUniRule annotation1
Active sitei131Proton donor/acceptorUniRule annotation1
Sitei163Transition state stabilizerUniRule annotation1
Sitei224Transition state stabilizerUniRule annotation1
Metal bindingi367MagnesiumUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi191 – 195ATPUniRule annotation5
Nucleotide bindingi265 – 267ATPUniRule annotation3
Nucleotide bindingi313 – 317ATPUniRule annotation5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMGIL350054:G1G8L-207-MONOMER
UniPathwayiUPA00340; UER00458

Names & Taxonomyi

Protein namesi
Recommended name:
Acetate kinaseUniRule annotation (EC:2.7.2.1UniRule annotation)
Alternative name(s):
AcetokinaseUniRule annotation
Gene namesi
Name:ackAUniRule annotation
Ordered Locus Names:Mflv_0204
OrganismiMycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 / PYR-GCK))
Taxonomic identifieri350054 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycolicibacterium
Proteomesi
  • UP000000232 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000899821 – 386Acetate kinaseAdd BLAST386

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi350054.Mflv_0204

Structurei

3D structure databases

ProteinModelPortaliA4T166
SMRiA4T166
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the acetokinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C6H Bacteria
COG0282 LUCA
HOGENOMiHOG000288399
KOiK00925
OMAiWKALTGT
OrthoDBiPOG091H02K4

Family and domain databases

HAMAPiMF_00020 Acetate_kinase, 1 hit
InterProiView protein in InterPro
IPR004372 Ac/propionate_kinase
IPR000890 Aliphatic_acid_kin_short-chain
IPR023865 Aliphatic_acid_kinase_CS
PANTHERiPTHR21060 PTHR21060, 1 hit
PfamiView protein in Pfam
PF00871 Acetate_kinase, 1 hit
PIRSFiPIRSF000722 Acetate_prop_kin, 1 hit
PRINTSiPR00471 ACETATEKNASE
TIGRFAMsiTIGR00016 ackA, 1 hit
PROSITEiView protein in PROSITE
PS01075 ACETATE_KINASE_1, 1 hit
PS01076 ACETATE_KINASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

A4T166-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTRTVLVLNS GSSSVKYAVL EPDSGVLIAD GIVERIGQEG GAHDHAAAMR
60 70 80 90 100
GVFDSLAADG HHLEDLGLVA VGHRVVHGGP DLYRPTIVDE TVIDRLKELG
110 120 130 140 150
PLAPLHNPPA VLGIEVARDA LPDLPHVAVF DTAFFHDLPA AAATYAIDAE
160 170 180 190 200
VARDWNIRRY GFHGTSHQYV SEQAAAFLDV PLESLSQIVL HLGNGASVSA
210 220 230 240 250
IVGGRPVETS MGLTPMEGLV MGTRSGDVDP GVIFYLWREA GMPVEDIESM
260 270 280 290 300
LNRRSGVRGL GGEIDFRELH RRIESGDDAA ELAYEVYIHR LRKYIGAYLA
310 320 330 340 350
ILGSADVITF TAGVGENDAV VRRDALTGLA AFGIEIDEHL NDSPGRGARR
360 370 380
ISADGAPTTV LVIPTDEELA IARACTGVLG ARPGDG
Length:386
Mass (Da):40,978
Last modified:May 15, 2007 - v1
Checksum:iCD75B3FA1174E968
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000656 Genomic DNA Translation: ABP42699.1
RefSeqiWP_011891206.1, NC_009338.1

Genome annotation databases

EnsemblBacteriaiABP42699; ABP42699; Mflv_0204
KEGGimgi:Mflv_0204

Similar proteinsi

Entry informationi

Entry nameiACKA_MYCGI
AccessioniPrimary (citable) accession number: A4T166
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: May 15, 2007
Last modified: June 20, 2018
This is version 70 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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