UniProtKB - A4Q9E8 (TTLL6_MOUSE)
Protein
Tubulin polyglutamylase TTLL6
Gene
Ttll6
Organism
Mus musculus (Mouse)
Status
Functioni
Polyglutamylase which preferentially modifies alpha-tubulin (PubMed:17499049, PubMed:21074048). Mediates tubulin polyglutamylation in cilia (PubMed:22246503). Involved in the side-chain elongation step of the polyglutamylation reaction rather than in the initiation step (PubMed:17499049, PubMed:21074048). Generates long side-chains (PubMed:20530212). Generates polyglutamylation of CGAS, leading to impair the DNA-binding activity of CGAS (PubMed:26829768).4 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 215 | ATPBy similarity | 1 | |
Binding sitei | 217 | ATPBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 202 – 205 | ATP bindingBy similarity | 4 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- protein-glutamic acid ligase activity Source: UniProtKB
- tubulin binding Source: UniProtKB
- tubulin-glutamic acid ligase activity Source: GO_Central
GO - Biological processi
- microtubule bundle formation Source: MGI
- microtubule cytoskeleton organization Source: GO_Central
- microtubule severing Source: MGI
- positive regulation of cilium movement Source: MGI
- protein polyglutamylation Source: UniProtKB
Keywordsi
Molecular function | Ligase |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-8955332, Carboxyterminal post-translational modifications of tubulin |
Names & Taxonomyi
Protein namesi | Recommended name: Tubulin polyglutamylase TTLL6 (EC:6.-.-.-)Alternative name(s): Tubulin--tyrosine ligase-like protein 6 |
Gene namesi | Name:Ttll6Imported |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2683461, Ttll6 |
Subcellular locationi
Cytoskeleton
- cilium basal body 1 Publication
Other locations
- cilium 1 Publication
Note: CEP41 is required for its transport between the basal body and the cilium.By similarity
Cytoskeleton
- ciliary basal body Source: UniProtKB
- microtubule Source: UniProtKB-KW
Other locations
Keywords - Cellular componenti
Cell projection, Cilium, Cytoplasm, Cytoskeleton, MicrotubulePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000326161 | 1 – 822 | Tubulin polyglutamylase TTLL6Add BLAST | 822 |
Proteomic databases
PaxDbi | A4Q9E8 |
PRIDEi | A4Q9E8 |
PTM databases
iPTMneti | A4Q9E8 |
PhosphoSitePlusi | A4Q9E8 |
Expressioni
Tissue specificityi
Widely expressed with highest levels in testis.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000038756, Expressed in spermatid and 85 other tissues |
Genevisiblei | A4Q9E8, MM |
Interactioni
Subunit structurei
Found in a complex with CEP41.
By similarityGO - Molecular functioni
- tubulin binding Source: UniProtKB
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000127778 |
Miscellaneous databases
RNActi | A4Q9E8, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 57 – 400 | TTLPROSITE-ProRule annotationAdd BLAST | 344 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 371 – 450 | c-MTBD regionBy similarityAdd BLAST | 80 |
Domaini
The flexible c-MTBD (cationic microtubule binding domain) region mediates binding to microtubules. It is positively charged and becomes ordered when bound to microtubules: it interacts with a negatively charged patch on tubulin. The presence of positive charges in the c-MTBD region is essential for proper binding.By similarity
Sequence similaritiesi
Belongs to the tubulin--tyrosine ligase family.Sequence analysis
Phylogenomic databases
eggNOGi | KOG2158, Eukaryota |
GeneTreei | ENSGT00940000161434 |
HOGENOMi | CLU_010131_7_2_1 |
InParanoidi | A4Q9E8 |
OMAi | AYPIIKH |
OrthoDBi | 1251554at2759 |
PhylomeDBi | A4Q9E8 |
TreeFami | TF313087 |
Family and domain databases
Gene3Di | 3.30.1490.20, 1 hit |
InterProi | View protein in InterPro IPR013815, ATP_grasp_subdomain_1 IPR004344, TTL/TTLL_fam IPR027754, TTLL6 |
PANTHERi | PTHR12241:SF96, PTHR12241:SF96, 1 hit |
Pfami | View protein in Pfam PF03133, TTL, 1 hit |
PROSITEi | View protein in PROSITE PS51221, TTL, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform 11 Publication (identifier: A4Q9E8-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MLQCLTSESE EGAEEREESS TEDLEELKEF VTLAFVRENT QKRLQNAQQH
60 70 80 90 100
GKKKRKKKRL VINLSNCRYD SVRRAAQQYG LREAGDNDDW TLYWTDYSVS
110 120 130 140 150
LERVMEMKSY QKINHFPGMS EICRKDLLAR NMSRMLKLFP KDFHFFPRTW
160 170 180 190 200
CLPADWGDLQ TYSRTRKNKT YICKPDSGCQ GRGIFITRSV KEIKPGEDMI
210 220 230 240 250
CQLYISKPFI IDGFKFDLRV YVLVTSCDPL RVFVYNEGLA RFATTSYSHP
260 270 280 290 300
NLDNLDEICM HLTNYSINKH SSNFVQDAFS GSKRKLSTFN SYMKTHGYDV
310 320 330 340 350
EQIWRGIEDV IIKTLISAHP VIKHNYHTCF PSHTLNSACF EILGFDILLD
360 370 380 390 400
RKLKPWLLEV NHSPSFSTDS KLDKEVKDSL LYDALVLINL GNCDKKKVLE
410 420 430 440 450
EERQRGRFLQ QCPNREIRLE EVKGFQAMRL QKTEEYEKKN CGGFRLIYPG
460 470 480 490 500
LNLEKYDKFF QDNSSLFQNT VASRARELYA RQLIQELRQK QEKKVFLKKA
510 520 530 540 550
RKEETQGESA GEQARDKVVR LQRQRQQPKC KTVATCPPKQ SLHPVTLVSC
560 570 580 590 600
TSGLLLNIRG LKKGEISESL EQKDTKEAML IPCKPVSARN YSSVPDLRSA
610 620 630 640 650
NPSCFEPEFH VPNAKVKEVK SAFMVNIEST AQPITSVESS RDATAPISTS
660 670 680 690 700
LESLASMSLS TSPECSSPES VHMVSYNHKQ QKASFHKPMQ EKKSKPLMFS
710 720 730 740 750
KSRHLDLNCT SMKNDINRQY LMSEILQKVQ MKKKRPLFPA PKSQYPTLSK
760 770 780 790 800
ERCPHSRSSS RKKEMNSPSV FVLQASHSRA ESLNDLLVVA TQARLDPRPS
810 820
RSHSGTTTRD SSTQDPKHTA TA
Sequence cautioni
The sequence BAC36577 differs from that shown. Reason: Frameshift.Curated
The sequence CAM18274 differs from that shown. Reason: Erroneous gene model prediction.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 195 | P → A in BAC36577 (PubMed:16141072).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_052729 | 1 – 104 | Missing in isoform 2. 2 PublicationsAdd BLAST | 104 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AM690749 mRNA Translation: CAM84326.1 AK077033 mRNA Translation: BAC36577.1 Frameshift. AK145771 mRNA Translation: BAE26641.1 AL603682 Genomic DNA Translation: CAM18273.1 AL603682 Genomic DNA Translation: CAM18274.1 Sequence problems. BC132203 mRNA Translation: AAI32204.1 BC138058 mRNA Translation: AAI38059.1 BC145131 mRNA Translation: AAI45132.1 |
CCDSi | CCDS25290.2 [A4Q9E8-1] |
RefSeqi | NP_766387.2, NM_172799.4 [A4Q9E8-1] XP_017170019.1, XM_017314530.1 XP_017170020.1, XM_017314531.1 [A4Q9E8-2] |
Genome annotation databases
Ensembli | ENSMUST00000107680; ENSMUSP00000103307; ENSMUSG00000038756 [A4Q9E8-2] ENSMUST00000167258; ENSMUSP00000127778; ENSMUSG00000038756 [A4Q9E8-1] |
GeneIDi | 237930 |
KEGGi | mmu:237930 |
UCSCi | uc011ydd.1, mouse [A4Q9E8-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AM690749 mRNA Translation: CAM84326.1 AK077033 mRNA Translation: BAC36577.1 Frameshift. AK145771 mRNA Translation: BAE26641.1 AL603682 Genomic DNA Translation: CAM18273.1 AL603682 Genomic DNA Translation: CAM18274.1 Sequence problems. BC132203 mRNA Translation: AAI32204.1 BC138058 mRNA Translation: AAI38059.1 BC145131 mRNA Translation: AAI45132.1 |
CCDSi | CCDS25290.2 [A4Q9E8-1] |
RefSeqi | NP_766387.2, NM_172799.4 [A4Q9E8-1] XP_017170019.1, XM_017314530.1 XP_017170020.1, XM_017314531.1 [A4Q9E8-2] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6VZQ | X-ray | 3.08 | A/B/C/D | 51-503 | [»] | |
6VZR | X-ray | 2.60 | A/B/C/D | 51-503 | [»] | |
6VZS | X-ray | 2.66 | A/B/C/D | 51-503 | [»] | |
6VZT | X-ray | 2.18 | A/B | 51-503 | [»] | |
6VZU | X-ray | 1.98 | A/B/C/D | 51-503 | [»] | |
6VZV | X-ray | 2.33 | A/B/C/D | 51-503 | [»] | |
6VZW | X-ray | 2.50 | A/B/C/D | 51-503 | [»] | |
SMRi | A4Q9E8 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000127778 |
PTM databases
iPTMneti | A4Q9E8 |
PhosphoSitePlusi | A4Q9E8 |
Proteomic databases
PaxDbi | A4Q9E8 |
PRIDEi | A4Q9E8 |
Protocols and materials databases
Antibodypediai | 17865, 38 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000107680; ENSMUSP00000103307; ENSMUSG00000038756 [A4Q9E8-2] ENSMUST00000167258; ENSMUSP00000127778; ENSMUSG00000038756 [A4Q9E8-1] |
GeneIDi | 237930 |
KEGGi | mmu:237930 |
UCSCi | uc011ydd.1, mouse [A4Q9E8-1] |
Organism-specific databases
CTDi | 284076 |
MGIi | MGI:2683461, Ttll6 |
Phylogenomic databases
eggNOGi | KOG2158, Eukaryota |
GeneTreei | ENSGT00940000161434 |
HOGENOMi | CLU_010131_7_2_1 |
InParanoidi | A4Q9E8 |
OMAi | AYPIIKH |
OrthoDBi | 1251554at2759 |
PhylomeDBi | A4Q9E8 |
TreeFami | TF313087 |
Enzyme and pathway databases
Reactomei | R-MMU-8955332, Carboxyterminal post-translational modifications of tubulin |
Miscellaneous databases
BioGRID-ORCSi | 237930, 1 hit in 17 CRISPR screens |
PROi | PR:A4Q9E8 |
RNActi | A4Q9E8, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000038756, Expressed in spermatid and 85 other tissues |
Genevisiblei | A4Q9E8, MM |
Family and domain databases
Gene3Di | 3.30.1490.20, 1 hit |
InterProi | View protein in InterPro IPR013815, ATP_grasp_subdomain_1 IPR004344, TTL/TTLL_fam IPR027754, TTLL6 |
PANTHERi | PTHR12241:SF96, PTHR12241:SF96, 1 hit |
Pfami | View protein in Pfam PF03133, TTL, 1 hit |
PROSITEi | View protein in PROSITE PS51221, TTL, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TTLL6_MOUSE | |
Accessioni | A4Q9E8Primary (citable) accession number: A4Q9E8 Secondary accession number(s): A2A6M6 Q8BVQ1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 18, 2008 |
Last sequence update: | May 15, 2007 | |
Last modified: | December 2, 2020 | |
This is version 102 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families