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Protein

Centrosomal protein of 295 kDa

Gene

Cep295

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Centriole-enriched microtubule-binding protein involved in centriole biogenesis. Essential for the generation of the distal portion of new-born centrioles in a CENPJ- and CEP120-mediated elongation dependent manner during the cell cycle S/G2 phase after formation of the initiating cartwheel structure. Required for the recruitment of centriolar proteins, such as POC1B, POC5 and CEP135, into the distal portion of centrioles. Also required for centriole-to-centrosome conversion during mitotic progression, but is dispensable for cartwheel removal or centriole disengagement. Binds to and stabilizes centriolar microtubule.By similarity

GO - Molecular functioni

GO - Biological processi

Names & Taxonomyi

Protein namesi
Recommended name:
Centrosomal protein of 295 kDaCurated
Gene namesi
Name:Cep295By similarity
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 8

Organism-specific databases

RGDi1311723 Cep295

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003245971 – 2395Centrosomal protein of 295 kDaAdd BLAST2395

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei13PhosphoserineBy similarity1
Modified residuei634PhosphoserineBy similarity1
Modified residuei1565PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiA4L9P8
PRIDEiA4L9P8

PTM databases

iPTMnetiA4L9P8
PhosphoSitePlusiA4L9P8

Expressioni

Gene expression databases

BgeeiENSRNOG00000010999 Expressed in 10 organ(s), highest expression level in testis
ExpressionAtlasiA4L9P8 baseline and differential
GenevisibleiA4L9P8 RN

Interactioni

Subunit structurei

Interacts (via ALMS motif) with microtubules; this interaction is direct.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000029431

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 540Necessary for centriole targeting and microtubule associationBy similarityAdd BLAST540
Regioni2329 – 2395ALMS motifAdd BLAST67

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili53 – 84Sequence analysisAdd BLAST32
Coiled coili114 – 148Sequence analysisAdd BLAST35
Coiled coili209 – 277Sequence analysisAdd BLAST69
Coiled coili488 – 538Sequence analysisAdd BLAST51
Coiled coili567 – 592Sequence analysisAdd BLAST26
Coiled coili817 – 848Sequence analysisAdd BLAST32
Coiled coili1444 – 1488Sequence analysisAdd BLAST45

Domaini

The N-terminal and the ALMS motif-containing C-terminal regions are essential for CEP295-mediated centriole elongation.By similarity

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IK6S Eukaryota
ENOG41124ZG LUCA
GeneTreeiENSGT00540000072076
HOGENOMiHOG000113176
HOVERGENiHBG108044
InParanoidiA4L9P8
PhylomeDBiA4L9P8
TreeFamiTF331536

Family and domain databases

InterProiView protein in InterPro
IPR029560 CEP295
PANTHERiPTHR21553:SF25 PTHR21553:SF25, 2 hits

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

A4L9P8-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKRKVMNGKL RLSPNEEAFI LKEDYERRRK LRLLQVREQE RGIAFQIRED
60 70 80 90 100
IKQRRNQQVS HLAEELRAEW EEAQSQKIQN LEKLYLASLR HMGDGHQQAK
110 120 130 140 150
ENEPDLDALS RRAAERKTKA EARHKEALKA QKKQKEMLMK QKTRHIKARK
160 170 180 190 200
EAVLVEKERS AKMARLPPPV PSPFENIDIN RIPSLKTNRS TYHHISAFVS
210 220 230 240 250
RQMGTKQPDA HLAAEEEARR VERLRKQAAQ ERMEQSERAH ARGSQAMKKI
260 270 280 290 300
HLAQNQERLM EELKQLQRED LACKRQTAAQ MPSQLLELPY RRSEMKEDWQ
310 320 330 340 350
RELEFAFEDV YSADRKVKGN LILHLKPEPL PTMSDQLQDE ELDLSMEQEN
360 370 380 390 400
EVPLATKTQQ IPSRILLKRL LNKIRNQKSL WTIKSFSEDD NQVTASIISE
410 420 430 440 450
IERKVPSTDS GTITTGETAV SFEQEQVMGS DRLMIESGPP SSEDKPLCYK
460 470 480 490 500
SVTGKEQAMG VSPPATTVAQ SSVLLHPQEE AARLRMSARH KQIMEIEEQK
510 520 530 540 550
QKQLELLEQI EQQKLRLETD CFQAQLEETR KQADHLEVRP APVSHAMISD
560 570 580 590 600
EERHRQMIRN YQYQLLQQNR LHKQTVETAR KRLLEYQTVL KERCPSMSAR
610 620 630 640 650
SLIPDSVVSE PPQQAQKPAV ASDYWDPSQR PKLSPSKYQP VQPSQIPALD
660 670 680 690 700
QNHIQVPRQG HIPQRQGETA RAKQSVESQE RQWQFSQVET QQRDYEFIFK
710 720 730 740 750
DSHSLSRTSS YVRPQTLQAA GEVSKPLRAI ICQTSDSQQI SSEDSENISS
760 770 780 790 800
KPTEPSSSLP LMPECSSSSL SVKLESETIQ KAFTTVNRSV ISQMHGQPLS
810 820 830 840 850
SSETGTTQQG DIRFLQGQLE LQKKVLQERQ EAQEKLLSCT QKELEEQTGI
860 870 880 890 900
PVFFPSPVGN MFSSLPSASA ESGNIQTSST KSDATVSSDS MDNPYSQPIS
910 920 930 940 950
LRQTNLEFLQ EQFSVEKDNL QARREAQEVS FTHTQSKLDK IVRSEQTGSS
960 970 980 990 1000
WPQLVALESF SSLTSADTQS RKIQKPPLPT NKKGLLPSQS EILSSQDGSS
1010 1020 1030 1040 1050
GFLQQTLPLQ NTLKLLQEQL TIQRGMIQPR LNAQETLLLH KERCSVDSKA
1060 1070 1080 1090 1100
GPVNSLSSAV AQHSEAGPQS LQELYSSKKE NTVLSSHLIT PEVQEESHGS
1110 1120 1130 1140 1150
PQHSLPRQEH FASLQEQAHI QRVILGARKQ IQEFAHKQNE FKKGLYSQQT
1160 1170 1180 1190 1200
GALSSPSQGT GWEISQESLS VRSDSTDPLS HFKIPGFQER LVRALQPTFP
1210 1220 1230 1240 1250
LRDNLQEHQE WVDPEKESFQ FSPQTQENRS SQQTGFSSFT PSLRQPSCVS
1260 1270 1280 1290 1300
LPSVDSGITQ HPLSTERDSK VTSSHLQIPE LQHRLLKISQ LIQPQQDSLK
1310 1320 1330 1340 1350
ALQEQLATSR TIIHSRQEAL EETLREWKEK IFPEQVGPFS PLMTQHSFAS
1360 1370 1380 1390 1400
FPVSDIERAQ ELCSTNSEGA ISSGYSEMLE LPDRALGLSC TALPQQGNLT
1410 1420 1430 1440 1450
VHPGHLHAQT NSFHSTEKAQ EKLVFPRPCK LEEISAEHSI QPPHDDLQAL
1460 1470 1480 1490 1500
QQQLDVHREA IRSCQDIQEE LLLQRLNKLE QRVSSKQISS SPLLSQVALP
1510 1520 1530 1540 1550
IANSEGTLQS SPAKNDDTEM LRSHSEYLNF SQPLQDNVTE QLDLEVVFHK
1560 1570 1580 1590 1600
ELLLHKQKSQ TKSESPEHAA PFNDAVIPRL QDRLLSYFQP ALTQQDSMSP
1610 1620 1630 1640 1650
QKQLNLQREA LYSRQKAQEE LLVQRQTALQ QQVQKHRETL KGFSNVSQTR
1660 1670 1680 1690 1700
AASGENDLEM QKTEQLTGWF PHIQGWPWGD SSQGSSSGDQ PGAAAVHAEH
1710 1720 1730 1740 1750
SGESLGKELS GRASKPPVSK VKCVFDLNQH ELSTIQEVES PTSGRTSMPG
1760 1770 1780 1790 1800
KAEFYRDRDP LRVSVSREQS YLGSPVAHDP FGCHQPSVQE NSKSHDTAKA
1810 1820 1830 1840 1850
VKVKKSDIED HALLSHAISE EEEEEEACTN LSPLMKPDDE VETQEISQEL
1860 1870 1880 1890 1900
LSSMTVSTGS FLSYEITDLS LTDPESFSEQ TEHQEQESSS KEEETGSLSC
1910 1920 1930 1940 1950
AVPSTQVTYQ QQHSLGAHNS LLPTEEENAS DQTHVHQIID KDINEANLIP
1960 1970 1980 1990 2000
DKRDFQVPAV DLDFPELEHL FPHLHRQLFK PLEPHLDLDL SSPGTSQEDR
2010 2020 2030 2040 2050
DFYQQNSESS SEKHVKALST STLCFTALTA GSHSPNSRLN QQLDVNLAHA
2060 2070 2080 2090 2100
TTEGSEQSFQ QLLPEFSSQE SQHTDLPSIY SIEARGTSQS MENQNYSEIL
2110 2120 2130 2140 2150
QNKKKSIYFQ PSTENLSPAC SSSDTTLFDQ LHPQHSTPCG SVSSEGSVKQ
2160 2170 2180 2190 2200
LEGREEMLGF EELSRRAVPM SQRLTEDENV VLPINPYVGT VEMETSIQGS
2210 2220 2230 2240 2250
NSLSIQNEKP IQNVIKTETT KAVRNVCQLA QEEHMLKSES CPFRRPIPVW
2260 2270 2280 2290 2300
ETETGYGIME EPDLTLLSTS DISITDTDLA NLTIEDNEAQ CSQAGAVQPS
2310 2320 2330 2340 2350
SSVETTFCGA ASEPWADQPT VASSAIPGSL GEAFMKRKKT FMERSYQRQR
2360 2370 2380 2390
EIWNKTPLPQ AKVSKEKLST SSSLSHLKEA VSGDETAKRN RSQCI
Length:2,395
Mass (Da):269,982
Last modified:March 18, 2008 - v2
Checksum:i4393391A96C9837B
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0G2K773A0A0G2K773_RAT
Centrosomal protein of 295 kDa
Cep295
2,473Annotation score:
A0A0G2K417A0A0G2K417_RAT
Centrosomal protein of 295 kDa
Cep295
2,342Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03062369 Genomic DNA No translation available.
EF460314 mRNA Translation: ABO47656.1
UniGeneiRn.160168

Genome annotation databases

EnsembliENSRNOT00000029634; ENSRNOP00000029431; ENSRNOG00000010999
UCSCiRGD:1311723 rat

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03062369 Genomic DNA No translation available.
EF460314 mRNA Translation: ABO47656.1
UniGeneiRn.160168

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000029431

PTM databases

iPTMnetiA4L9P8
PhosphoSitePlusiA4L9P8

Proteomic databases

PaxDbiA4L9P8
PRIDEiA4L9P8

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000029634; ENSRNOP00000029431; ENSRNOG00000010999
UCSCiRGD:1311723 rat

Organism-specific databases

RGDi1311723 Cep295

Phylogenomic databases

eggNOGiENOG410IK6S Eukaryota
ENOG41124ZG LUCA
GeneTreeiENSGT00540000072076
HOGENOMiHOG000113176
HOVERGENiHBG108044
InParanoidiA4L9P8
PhylomeDBiA4L9P8
TreeFamiTF331536

Miscellaneous databases

PROiPR:A4L9P8

Gene expression databases

BgeeiENSRNOG00000010999 Expressed in 10 organ(s), highest expression level in testis
ExpressionAtlasiA4L9P8 baseline and differential
GenevisibleiA4L9P8 RN

Family and domain databases

InterProiView protein in InterPro
IPR029560 CEP295
PANTHERiPTHR21553:SF25 PTHR21553:SF25, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiCE295_RAT
AccessioniPrimary (citable) accession number: A4L9P8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: March 18, 2008
Last modified: September 12, 2018
This is version 59 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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