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Protein

S-adenosylmethionine synthase

Gene

metK

Organism
Francisella tularensis subsp. tularensis (strain WY96-3418)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of S-adenosylmethionine (AdoMet) from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme.UniRule annotation

Catalytic activityi

ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine.UniRule annotation

Cofactori

Protein has several cofactor binding sites:

Pathwayi: S-adenosyl-L-methionine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes S-adenosyl-L-methionine from L-methionine.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. S-adenosylmethionine synthase (metK)
This subpathway is part of the pathway S-adenosyl-L-methionine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes S-adenosyl-L-methionine from L-methionine, the pathway S-adenosyl-L-methionine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei16ATPUniRule annotation1
Metal bindingi18MagnesiumUniRule annotation1
Metal bindingi44PotassiumUniRule annotation1
Binding sitei57MethionineUniRule annotation1
Binding sitei100MethionineUniRule annotation1
Binding sitei240ATP; shared with neighboring subunitUniRule annotation1
Binding sitei240Methionine; shared with neighboring subunitUniRule annotation1
Binding sitei263ATP; via amide nitrogen; shared with neighboring subunitUniRule annotation1
Binding sitei267ATP; shared with neighboring subunitUniRule annotation1
Binding sitei271MethionineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi165 – 167ATPUniRule annotation3
Nucleotide bindingi246 – 247ATPUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processOne-carbon metabolism
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium

Enzyme and pathway databases

BioCyciFTUL418136:G1G8F-230-MONOMER
UniPathwayi
UPA00315;UER00080

Names & Taxonomyi

Protein namesi
Recommended name:
S-adenosylmethionine synthaseUniRule annotation (EC:2.5.1.6UniRule annotation)
Short name:
AdoMet synthaseUniRule annotation
Alternative name(s):
MATUniRule annotation
Methionine adenosyltransferaseUniRule annotation
Gene namesi
Name:metKUniRule annotation
Ordered Locus Names:FTW_0239
OrganismiFrancisella tularensis subsp. tularensis (strain WY96-3418)
Taxonomic identifieri418136 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaThiotrichalesFrancisellaceaeFrancisella

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003029171 – 386S-adenosylmethionine synthaseAdd BLAST386

Interactioni

Subunit structurei

Homotetramer; dimer of dimers.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA4IWA4
SMRiA4IWA4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni100 – 110Flexible loopUniRule annotationAdd BLAST11

Sequence similaritiesi

Belongs to the AdoMet synthase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000245710
KOiK00789
OMAiPGHFLFT

Family and domain databases

HAMAPiMF_00086 S_AdoMet_synth1, 1 hit
InterProiView protein in InterPro
IPR022631 ADOMET_SYNTHASE_CS
IPR022630 S-AdoMet_synt_C
IPR022629 S-AdoMet_synt_central
IPR022628 S-AdoMet_synt_N
IPR002133 S-AdoMet_synthetase
IPR022636 S-AdoMet_synthetase_sfam
PANTHERiPTHR11964 PTHR11964, 1 hit
PfamiView protein in Pfam
PF02773 S-AdoMet_synt_C, 1 hit
PF02772 S-AdoMet_synt_M, 1 hit
PF00438 S-AdoMet_synt_N, 1 hit
PIRSFiPIRSF000497 MAT, 1 hit
SUPFAMiSSF55973 SSF55973, 3 hits
TIGRFAMsiTIGR01034 metK, 1 hit
PROSITEiView protein in PROSITE
PS00376 ADOMET_SYNTHASE_1, 1 hit
PS00377 ADOMET_SYNTHASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

A4IWA4-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSKNYLFTSE SVSEGHPDKL ADQISDAILD EILKQDKNAR VACETLVKTG
60 70 80 90 100
MALVAGEITT SAWVDIKELV RNVITETGYD NASKGIDGRT CSVINAIDKQ
110 120 130 140 150
SRDITQGVDR GSLEDLGAGD QGLMFGFATN ETPTLMPSAI YYSHLLMRKQ
160 170 180 190 200
AELRKSGKLA WLRPDAKAQV TLAYENDKPK FIDTIVLSTQ HNESISQKEL
210 220 230 240 250
HDAVIEEIVK KVIPNELITK DTKYHINPTG VFLIGGPQGD CGLTGRKIIV
260 270 280 290 300
DTYGGAAHHG GGAFSGKDPS KVDRSGAYMG RYIAKNIVAA GLADKCEVQV
310 320 330 340 350
AYAIGVAKPV SLMVNTFGTG KITDNQIEKL VAEVFDLRVG KIIENLDLLR
360 370 380
PIYRKTSNYG HFGRELPEFT WEKIDKADIL KSAARI
Length:386
Mass (Da):42,190
Last modified:May 1, 2007 - v1
Checksum:iF3D7343FD5C72EA5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000608 Genomic DNA Translation: ABO46206.1
RefSeqiWP_003024819.1, NC_009257.1

Genome annotation databases

EnsemblBacteriaiABO46206; ABO46206; FTW_0239
KEGGiftw:FTW_0239

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000608 Genomic DNA Translation: ABO46206.1
RefSeqiWP_003024819.1, NC_009257.1

3D structure databases

ProteinModelPortaliA4IWA4
SMRiA4IWA4
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABO46206; ABO46206; FTW_0239
KEGGiftw:FTW_0239

Phylogenomic databases

HOGENOMiHOG000245710
KOiK00789
OMAiPGHFLFT

Enzyme and pathway databases

UniPathwayi
UPA00315;UER00080

BioCyciFTUL418136:G1G8F-230-MONOMER

Family and domain databases

HAMAPiMF_00086 S_AdoMet_synth1, 1 hit
InterProiView protein in InterPro
IPR022631 ADOMET_SYNTHASE_CS
IPR022630 S-AdoMet_synt_C
IPR022629 S-AdoMet_synt_central
IPR022628 S-AdoMet_synt_N
IPR002133 S-AdoMet_synthetase
IPR022636 S-AdoMet_synthetase_sfam
PANTHERiPTHR11964 PTHR11964, 1 hit
PfamiView protein in Pfam
PF02773 S-AdoMet_synt_C, 1 hit
PF02772 S-AdoMet_synt_M, 1 hit
PF00438 S-AdoMet_synt_N, 1 hit
PIRSFiPIRSF000497 MAT, 1 hit
SUPFAMiSSF55973 SSF55973, 3 hits
TIGRFAMsiTIGR01034 metK, 1 hit
PROSITEiView protein in PROSITE
PS00376 ADOMET_SYNTHASE_1, 1 hit
PS00377 ADOMET_SYNTHASE_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMETK_FRATW
AccessioniPrimary (citable) accession number: A4IWA4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: May 1, 2007
Last modified: October 10, 2018
This is version 70 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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