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Protein

Chaperone protein DnaJ

Gene

dnaJ

Organism
Geobacillus thermodenitrificans (strain NG80-2)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 2 Zn2+ ions per monomer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi149Zinc 1UniRule annotation1
Metal bindingi152Zinc 1UniRule annotation1
Metal bindingi166Zinc 2UniRule annotation1
Metal bindingi169Zinc 2UniRule annotation1
Metal bindingi192Zinc 2UniRule annotation1
Metal bindingi195Zinc 2UniRule annotation1
Metal bindingi206Zinc 1UniRule annotation1
Metal bindingi209Zinc 1UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri136 – 218CR-typeUniRule annotationAdd BLAST83

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChaperone
Biological processDNA replication, Stress response
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Chaperone protein DnaJUniRule annotation
Gene namesi
Name:dnaJUniRule annotation
Ordered Locus Names:GTNG_2439
OrganismiGeobacillus thermodenitrificans (strain NG80-2)
Taxonomic identifieri420246 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus
Proteomesi
  • UP000001578 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000851991 – 381Chaperone protein DnaJAdd BLAST381

Interactioni

Subunit structurei

Homodimer.UniRule annotation

GO - Molecular functioni

Protein-protein interaction databases

STRINGi420246.GTNG_2439

Structurei

3D structure databases

ProteinModelPortaliA4IR30
SMRiA4IR30
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 69JUniRule annotationAdd BLAST65
Repeati149 – 156CXXCXGXG motif8
Repeati166 – 173CXXCXGXG motif8
Repeati192 – 199CXXCXGXG motif8
Repeati206 – 213CXXCXGXG motif8

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi76 – 119Gly-richAdd BLAST44

Domaini

The J domain is necessary and sufficient to stimulate DnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, DnaK-independent chaperone activity of DnaJ. Zinc center 2 is essential for interaction with DnaK and for DnaJ activity.UniRule annotation

Sequence similaritiesi

Belongs to the DnaJ family.UniRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri136 – 218CR-typeUniRule annotationAdd BLAST83

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG4105BZ5 Bacteria
COG0484 LUCA
HOGENOMiHOG000226717
KOiK03686
OMAiDMGGFAD
OrthoDBiPOG091H00PT

Family and domain databases

CDDicd06257 DnaJ, 1 hit
cd10719 DnaJ_zf, 1 hit
Gene3Di1.10.287.110, 1 hit
HAMAPiMF_01152 DnaJ, 1 hit
InterProiView protein in InterPro
IPR012724 DnaJ
IPR002939 DnaJ_C
IPR001623 DnaJ_domain
IPR018253 DnaJ_domain_CS
IPR008971 HSP40/DnaJ_pept-bd
IPR001305 HSP_DnaJ_Cys-rich_dom
IPR036410 HSP_DnaJ_Cys-rich_dom_sf
IPR036869 J_dom_sf
PfamiView protein in Pfam
PF00226 DnaJ, 1 hit
PF01556 DnaJ_C, 1 hit
PF00684 DnaJ_CXXCXGXG, 1 hit
PRINTSiPR00625 JDOMAIN
SMARTiView protein in SMART
SM00271 DnaJ, 1 hit
SUPFAMiSSF46565 SSF46565, 1 hit
SSF49493 SSF49493, 3 hits
SSF57938 SSF57938, 1 hit
TIGRFAMsiTIGR02349 DnaJ_bact, 1 hit
PROSITEiView protein in PROSITE
PS00636 DNAJ_1, 1 hit
PS50076 DNAJ_2, 1 hit
PS51188 ZF_CR, 1 hit

Sequencei

Sequence statusi: Complete.

A4IR30-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAKRDYYEVL GVSKNATKDE IKKAYRKLSK QYHPDINKAP DAAEKFKEIK
60 70 80 90 100
EAYEVLSDDE KRARYDRFGH ADPNEAFGGG FQGGGFDFGG FSGFGGFEDI
110 120 130 140 150
FETFFGGGSR RRASGPRKGA DLEYMMTLTF EEAAFGKETE IEVPHEETCD
160 170 180 190 200
TCHGSGAKPG TSPQSCPHCH GSGQVTSEQA TPFGRIVNRR TCPVCGGTGR
210 220 230 240 250
HIPEKCPTCG GTGHVKKRKK IHVKIPAGVD DGQQLRVAGK GEPGVNGGPP
260 270 280 290 300
GDLYIIFRVQ PHEFFKRDGD DIYCEVPLSF AQAALGDEIE VPTLHGDVKL
310 320 330 340 350
KIPAGTQTGT RFRLKGKGVP NVRGYGQGDQ HVIVRVVTPT KLTEKQKQLL
360 370 380
REFDRLGGET MHDGSHGRFF EKVKKAFKGE T
Length:381
Mass (Da):41,758
Last modified:May 1, 2007 - v1
Checksum:i679FEAD0E5BDB6F4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000557 Genomic DNA Translation: ABO67784.1
RefSeqiWP_008879918.1, NC_009328.1

Genome annotation databases

EnsemblBacteriaiABO67784; ABO67784; GTNG_2439
GeneIDi31761683
KEGGigtn:GTNG_2439

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000557 Genomic DNA Translation: ABO67784.1
RefSeqiWP_008879918.1, NC_009328.1

3D structure databases

ProteinModelPortaliA4IR30
SMRiA4IR30
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi420246.GTNG_2439

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABO67784; ABO67784; GTNG_2439
GeneIDi31761683
KEGGigtn:GTNG_2439

Phylogenomic databases

eggNOGiENOG4105BZ5 Bacteria
COG0484 LUCA
HOGENOMiHOG000226717
KOiK03686
OMAiDMGGFAD
OrthoDBiPOG091H00PT

Family and domain databases

CDDicd06257 DnaJ, 1 hit
cd10719 DnaJ_zf, 1 hit
Gene3Di1.10.287.110, 1 hit
HAMAPiMF_01152 DnaJ, 1 hit
InterProiView protein in InterPro
IPR012724 DnaJ
IPR002939 DnaJ_C
IPR001623 DnaJ_domain
IPR018253 DnaJ_domain_CS
IPR008971 HSP40/DnaJ_pept-bd
IPR001305 HSP_DnaJ_Cys-rich_dom
IPR036410 HSP_DnaJ_Cys-rich_dom_sf
IPR036869 J_dom_sf
PfamiView protein in Pfam
PF00226 DnaJ, 1 hit
PF01556 DnaJ_C, 1 hit
PF00684 DnaJ_CXXCXGXG, 1 hit
PRINTSiPR00625 JDOMAIN
SMARTiView protein in SMART
SM00271 DnaJ, 1 hit
SUPFAMiSSF46565 SSF46565, 1 hit
SSF49493 SSF49493, 3 hits
SSF57938 SSF57938, 1 hit
TIGRFAMsiTIGR02349 DnaJ_bact, 1 hit
PROSITEiView protein in PROSITE
PS00636 DNAJ_1, 1 hit
PS50076 DNAJ_2, 1 hit
PS51188 ZF_CR, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDNAJ_GEOTN
AccessioniPrimary (citable) accession number: A4IR30
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 1, 2007
Last modified: October 10, 2018
This is version 81 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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