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Protein

tRNA dimethylallyltransferase

Gene

miaA

Organism
Geobacillus thermodenitrificans (strain NG80-2)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i6A).UniRule annotation

Catalytic activityi

Dimethylallyl diphosphate + adenine(37) in tRNA = diphosphate + N6-dimethylallyladenine(37) in tRNA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 17ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processtRNA processing
LigandATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BioCyciGTHE420246:G1G82-1266-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA dimethylallyltransferaseUniRule annotation (EC:2.5.1.75UniRule annotation)
Alternative name(s):
Dimethylallyl diphosphate:tRNA dimethylallyltransferaseUniRule annotation
Short name:
DMAPP:tRNA dimethylallyltransferaseUniRule annotation
Short name:
DMATaseUniRule annotation
Isopentenyl-diphosphate:tRNA isopentenyltransferaseUniRule annotation
Short name:
IPP transferaseUniRule annotation
Short name:
IPPTUniRule annotation
Short name:
IPTaseUniRule annotation
Gene namesi
Name:miaAUniRule annotation
Ordered Locus Names:GTNG_1167
OrganismiGeobacillus thermodenitrificans (strain NG80-2)
Taxonomic identifieri420246 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus
Proteomesi
  • UP000001578 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000206041 – 315tRNA dimethylallyltransferaseAdd BLAST315

Proteomic databases

PRIDEiA4IMI5

Interactioni

Subunit structurei

Monomer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei101Interaction with substrate tRNAUniRule annotation1
Sitei124Interaction with substrate tRNAUniRule annotation1

Protein-protein interaction databases

STRINGi420246.GTNG_1167

Structurei

3D structure databases

ProteinModelPortaliA4IMI5
SMRiA4IMI5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni12 – 17Substrate bindingUniRule annotation6
Regioni35 – 38Interaction with substrate tRNAUniRule annotation4

Sequence similaritiesi

Belongs to the IPP transferase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DKX Bacteria
COG0324 LUCA
HOGENOMiHOG000039995
KOiK00791
OMAiGTGLYIR
OrthoDBiPOG091H00NK

Family and domain databases

HAMAPiMF_00185 IPP_trans, 1 hit
InterProiView protein in InterPro
IPR018022 IPT
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF01715 IPPT, 1 hit
SUPFAMiSSF52540 SSF52540, 4 hits
TIGRFAMsiTIGR00174 miaA, 1 hit

Sequencei

Sequence statusi: Complete.

A4IMI5-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTEKIVVIVG PTEVGKTKLG IALAKKLGGE IISGDSMQIY KGMDIGTAKV
60 70 80 90 100
KPDEMEGVPH HLLDIKEPCE PFSVVEFQRL ARALITDISA RGRLPIIVGG
110 120 130 140 150
TGLYIQAAIY DYRFSDAPSD EAYRRALRQL AAEQGAEALH EQLKAVDPES
160 170 180 190 200
AARIHPNNVR RVIRALEVYH CTGKPFSEWQ RGQQRQLLYE TALIGLTAER
210 220 230 240 250
SVLYRRINER VDEMIAEGLI EEVRSLYDRG LRDCQAVQAI GYKELYDYFD
260 270 280 290 300
GRVSLDEAIE QLKQNSRRYA KRQLTWFRNQ MPVEWFDMTD PEKFAVKVEE
310
IFRYIAGKLR LEANI
Length:315
Mass (Da):35,934
Last modified:May 1, 2007 - v1
Checksum:iF5048CF85B76A97F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000557 Genomic DNA Translation: ABO66539.1
RefSeqiWP_011887177.1, NC_009328.1

Genome annotation databases

EnsemblBacteriaiABO66539; ABO66539; GTNG_1167
GeneIDi31760427
KEGGigtn:GTNG_1167

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000557 Genomic DNA Translation: ABO66539.1
RefSeqiWP_011887177.1, NC_009328.1

3D structure databases

ProteinModelPortaliA4IMI5
SMRiA4IMI5
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi420246.GTNG_1167

Proteomic databases

PRIDEiA4IMI5

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABO66539; ABO66539; GTNG_1167
GeneIDi31760427
KEGGigtn:GTNG_1167

Phylogenomic databases

eggNOGiENOG4105DKX Bacteria
COG0324 LUCA
HOGENOMiHOG000039995
KOiK00791
OMAiGTGLYIR
OrthoDBiPOG091H00NK

Enzyme and pathway databases

BioCyciGTHE420246:G1G82-1266-MONOMER

Family and domain databases

HAMAPiMF_00185 IPP_trans, 1 hit
InterProiView protein in InterPro
IPR018022 IPT
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF01715 IPPT, 1 hit
SUPFAMiSSF52540 SSF52540, 4 hits
TIGRFAMsiTIGR00174 miaA, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMIAA_GEOTN
AccessioniPrimary (citable) accession number: A4IMI5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 1, 2007
Last modified: October 10, 2018
This is version 76 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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