UniProtKB - A4IJG0 (MCSB_GEOTN)
Protein
Protein-arginine kinase
Gene
mcsB
Organism
Geobacillus thermodenitrificans (strain NG80-2)
Status
Functioni
Catalyzes the specific phosphorylation of arginine residues in a large number of proteins. Is part of the bacterial stress response system. Protein arginine phosphorylation has a physiologically important role and is involved in the regulation of many critical cellular processes, such as protein homeostasis, motility, competence, and stringent and stress responses, by regulating gene expression and protein activity.UniRule annotation
Catalytic activityi
- EC:2.7.14.1UniRule annotation
Activity regulationi
Appears to be allosterically activated by the binding of pArg-containing polypeptides to the pArg-binding pocket localized in the C-terminal domain of McsB.UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 92 | ATPUniRule annotation | 1 | |
Binding sitei | 126 | ATPUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 27 – 31 | ATPUniRule annotation | 5 | |
Nucleotide bindingi | 177 – 181 | ATPUniRule annotation | 5 | |
Nucleotide bindingi | 208 – 213 | ATPUniRule annotation | 6 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- creatine kinase activity Source: InterPro
- protein kinase activity Source: UniProtKB-UniRule
GO - Biological processi
- phosphocreatine biosynthetic process Source: InterPro
Keywordsi
Molecular function | Allosteric enzyme, Kinase, Transferase |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | GTHE420246:G1G82-101-MONOMER |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:mcsBUniRule annotation Ordered Locus Names:GTNG_0077 |
Organismi | Geobacillus thermodenitrificans (strain NG80-2) |
Taxonomic identifieri | 420246 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Geobacillus › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_1000025875 | 1 – 363 | Protein-arginine kinaseAdd BLAST | 363 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 24 – 255 | Phosphagen kinase C-terminalUniRule annotationAdd BLAST | 232 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 338 – 343 | RDXXRA motif of the pArg binding pocket involved in allosteric regulationUniRule annotation | 6 |
Sequence similaritiesi
Belongs to the ATP:guanido phosphotransferase family.UniRule annotation
Phylogenomic databases
eggNOGi | COG3869, Bacteria |
HOGENOMi | CLU_066591_1_0_9 |
OMAi | NMREVFR |
OrthoDBi | 904915at2 |
Family and domain databases
CDDi | cd07930, bacterial_phosphagen_kinase, 1 hit |
HAMAPi | MF_00602, Prot_Arg_kinase, 1 hit |
InterProi | View protein in InterPro IPR023660, Arg_Kinase IPR000749, ATP-guanido_PTrfase IPR022415, ATP-guanido_PTrfase_AS IPR022414, ATP-guanido_PTrfase_cat IPR014746, Gln_synth/guanido_kin_cat_dom |
PANTHERi | PTHR11547, PTHR11547, 1 hit |
Pfami | View protein in Pfam PF00217, ATP-gua_Ptrans, 1 hit |
SUPFAMi | SSF55931, SSF55931, 1 hit |
PROSITEi | View protein in PROSITE PS00112, PHOSPHAGEN_KINASE, 1 hit PS51510, PHOSPHAGEN_KINASE_C, 1 hit |
i Sequence
Sequence statusi: Complete.
A4IJG0-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSFEKFFNTA VSAWMSQEGP DSDIVLSSRI RLARNIVDFR FPTLFSSEEA
60 70 80 90 100
MQIVALFERT FAYRSYGGAG RFELLKMSEL QPIEKRVLVE KHLISPHLAE
110 120 130 140 150
DSPFGACLLS ENEEISIMIN EEDHIRIQCL FPGLQLAEAL EAASELDDWI
160 170 180 190 200
EGHVNYAFDE RLGYLTSCPT NVGTGLRASV MMHLPALVLT QQINRIIPAI
210 220 230 240 250
NQLGLVVRGT YGEGSEALGN IFQISNQLTL GKSEEDIIAD LHTIVQQLIA
260 270 280 290 300
QERAARQALV KTLGIQLEDK VFRSYGILAN CRVIESKEAA QCLSDVRLGI
310 320 330 340 350
DLGYIKNVSR NILNELMILT QPGFLQQYAG GALRPEERDV RRAALIRERL
360
KMEERRKMEG DER
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000557 Genomic DNA Translation: ABO65464.1 |
RefSeqi | WP_011886620.1, NC_009328.1 |
Genome annotation databases
EnsemblBacteriai | ABO65464; ABO65464; GTNG_0077 |
KEGGi | gtn:GTNG_0077 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000557 Genomic DNA Translation: ABO65464.1 |
RefSeqi | WP_011886620.1, NC_009328.1 |
3D structure databases
SMRi | A4IJG0 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 420246.GTNG_0077 |
Genome annotation databases
EnsemblBacteriai | ABO65464; ABO65464; GTNG_0077 |
KEGGi | gtn:GTNG_0077 |
Phylogenomic databases
eggNOGi | COG3869, Bacteria |
HOGENOMi | CLU_066591_1_0_9 |
OMAi | NMREVFR |
OrthoDBi | 904915at2 |
Enzyme and pathway databases
BioCyci | GTHE420246:G1G82-101-MONOMER |
Family and domain databases
CDDi | cd07930, bacterial_phosphagen_kinase, 1 hit |
HAMAPi | MF_00602, Prot_Arg_kinase, 1 hit |
InterProi | View protein in InterPro IPR023660, Arg_Kinase IPR000749, ATP-guanido_PTrfase IPR022415, ATP-guanido_PTrfase_AS IPR022414, ATP-guanido_PTrfase_cat IPR014746, Gln_synth/guanido_kin_cat_dom |
PANTHERi | PTHR11547, PTHR11547, 1 hit |
Pfami | View protein in Pfam PF00217, ATP-gua_Ptrans, 1 hit |
SUPFAMi | SSF55931, SSF55931, 1 hit |
PROSITEi | View protein in PROSITE PS00112, PHOSPHAGEN_KINASE, 1 hit PS51510, PHOSPHAGEN_KINASE_C, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MCSB_GEOTN | |
Accessioni | A4IJG0Primary (citable) accession number: A4IJG0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 15, 2008 |
Last sequence update: | May 1, 2007 | |
Last modified: | April 7, 2021 | |
This is version 85 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |