UniProtKB - A4IGH4 (WDR4_DANRE)
tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit wdr4
wdr4
Functioni
Non-catalytic component of a methyltransferase complex required for the formation of N7-methylguanine in a subset of RNA species, such as tRNAs, mRNAs and microRNAs (miRNAs). In the methyltransferase complex, it is required to stabilize and induce conformational changes of the catalytic subunit. Required for the formation of N7-methylguanine at position 46 (m7G46) in tRNA. Also required for the formation of N7-methylguanine at internal sites in a subset of mRNAs. Also required for methylation of a specific subset of miRNAs.
UniRule annotation: N(7)-methylguanine-tRNA biosynthesis Pathwayi
This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotationView all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.
GO - Biological processi
- tRNA (guanine-N7)-methylation Source: GOC
- tRNA modification Source: UniProtKB
Keywordsi
Biological process | tRNA processing |
Enzyme and pathway databases
UniPathwayi | UPA00989 |
Names & Taxonomyi
Protein namesi | Recommended name: tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit wdr4UniRule annotationAlternative name(s): WD repeat-containing protein 4UniRule annotation |
Gene namesi | Name:wdr4 ORF Names:si:ch211-140m22.6 |
Organismi | Danio rerio (Zebrafish) (Brachydanio rerio) |
Taxonomic identifieri | 7955 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Danionidae › Danioninae › Danio |
Proteomesi |
|
Organism-specific databases
ZFINi | ZDB-GENE-060810-10, wdr4 |
Subcellular locationi
Nucleus
- Nucleus UniRule annotation
Cytosol
- cytosol Source: GO_Central
Nucleus
- nucleus Source: UniProtKB
Other locations
- tRNA methyltransferase complex Source: GO_Central
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000370533 | 1 – 413 | tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit wdr4Add BLAST | 413 |
Proteomic databases
PaxDbi | A4IGH4 |
PeptideAtlasi | A4IGH4 |
Interactioni
Subunit structurei
Forms a heterodimer with the catalytic subunit mettl1.
UniRule annotationProtein-protein interaction databases
STRINGi | 7955.ENSDARP00000103752 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 101 – 140 | WD 1Add BLAST | 40 | |
Repeati | 144 – 183 | WD 2Add BLAST | 40 | |
Repeati | 187 – 227 | WD 3Add BLAST | 41 | |
Repeati | 286 – 328 | WD 4Add BLAST | 43 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 386 – 413 | DisorderedSequence analysisAdd BLAST | 28 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 396 – 413 | Polar residuesSequence analysisAdd BLAST | 18 |
Sequence similaritiesi
Keywords - Domaini
Repeat, WD repeatPhylogenomic databases
eggNOGi | KOG3914, Eukaryota |
InParanoidi | A4IGH4 |
PhylomeDBi | A4IGH4 |
TreeFami | TF105877 |
Family and domain databases
Gene3Di | 2.130.10.10, 1 hit |
HAMAPi | MF_03056, TRM82, 1 hit |
InterProi | View protein in InterPro IPR028884, Trm82 IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR036322, WD40_repeat_dom_sf |
PANTHERi | PTHR16288, PTHR16288, 1 hit |
SMARTi | View protein in SMART SM00320, WD40, 3 hits |
SUPFAMi | SSF50978, SSF50978, 1 hit |
PROSITEi | View protein in PROSITE PS50082, WD_REPEATS_2, 1 hit PS50294, WD_REPEATS_REGION, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MAVVCSKADW FVTSCSTTLV AVNLKQSREP FVFDCSKAEK KPKEADVDNK
60 70 80 90 100
SAGEGSEEKD SDSILAFAIS ASGKHVALTD DHKRLVLFCT EPSWKCISTR
110 120 130 140 150
WVVRRCTSLA FTQAEDELYV ADKSGDVYSF SILEPHKAGE LKLGHLSMLL
160 170 180 190 200
DVALSPDDKY IITADRDEKI RVSFRRSPYN IQAFCLGHTE FVSSLLVPAG
210 220 230 240 250
HPDWLLSGSG DGTVNVWHYE TGRRLHSVDM RKFGLDSENT EKRFAVSRII
260 270 280 290 300
SSPDGQHVAV QCEGFPSVQL FTVDCGTEGL LKPADTLTLP LSPWDVTFDS
310 320 330 340 350
ENQLWVLLES EDMKVLLYRH SEQHWRLCDS ESPELKKVTN ALQTQWHLFK
360 370 380 390 400
GSVGLESQFK HLYKVNFDNM ASYLQKKQER LDLEHKKRAA AANGSKPNKK
410
SKTESGAVPQ STS
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketE7EZ59 | E7EZ59_DANRE | tRNA (guanine-N(7)-)-methyltransfer... | wdr4 WDR4 | 413 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 89 | C → G in AAI35102 (Ref. 2) Curated | 1 | |
Sequence conflicti | 279 | G → S in AAI35102 (Ref. 2) Curated | 1 | |
Sequence conflicti | 385 | H → N in AAI35102 (Ref. 2) Curated | 1 | |
Sequence conflicti | 407 | A → P in AAI35102 (Ref. 2) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CR361548 Genomic DNA Translation: CAQ13728.1 BC135101 mRNA Translation: AAI35102.1 Different initiation. |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CR361548 Genomic DNA Translation: CAQ13728.1 BC135101 mRNA Translation: AAI35102.1 Different initiation. |
3D structure databases
SMRi | A4IGH4 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 7955.ENSDARP00000103752 |
Proteomic databases
PaxDbi | A4IGH4 |
PeptideAtlasi | A4IGH4 |
Organism-specific databases
ZFINi | ZDB-GENE-060810-10, wdr4 |
Phylogenomic databases
eggNOGi | KOG3914, Eukaryota |
InParanoidi | A4IGH4 |
PhylomeDBi | A4IGH4 |
TreeFami | TF105877 |
Enzyme and pathway databases
UniPathwayi | UPA00989 |
Miscellaneous databases
PROi | PR:A4IGH4 |
Family and domain databases
Gene3Di | 2.130.10.10, 1 hit |
HAMAPi | MF_03056, TRM82, 1 hit |
InterProi | View protein in InterPro IPR028884, Trm82 IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR036322, WD40_repeat_dom_sf |
PANTHERi | PTHR16288, PTHR16288, 1 hit |
SMARTi | View protein in SMART SM00320, WD40, 3 hits |
SUPFAMi | SSF50978, SSF50978, 1 hit |
PROSITEi | View protein in PROSITE PS50082, WD_REPEATS_2, 1 hit PS50294, WD_REPEATS_REGION, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | WDR4_DANRE | |
Accessioni | A4IGH4Primary (citable) accession number: A4IGH4 Secondary accession number(s): B0UXG2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 5, 2009 |
Last sequence update: | May 5, 2009 | |
Last modified: | September 29, 2021 | |
This is version 88 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families